| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,192,380 | T→G | E84D (GAA→GAC) | hflD ← | putative lysogenization regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,192,380 | 0 | T | G | 95.2% | 126.4 / ‑4.2 | 42 | E84D (GAA→GAC) | hflD | putative lysogenization regulator |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (20/20); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTG > NC_000913/1192148‑1192576 | gtgGTCTTTGCAGTACAGCCGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCg < 2:9972/236‑1 (MQ=255) tgGGCTTTGCAGTTCAACAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCgg < 1:62191/236‑1 (MQ=255) cTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAACGGGTTAATGCGGCGtt > 2:632150/1‑236 (MQ=255) tGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTa > 2:457053/1‑235 (MQ=255) aGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGcc < 2:506297/236‑1 (MQ=255) cagcagGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGAACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGACCTg > 1:557990/1‑235 (MQ=255) cagGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCG‑TTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTg > 1:649254/1‑231 (MQ=255) aCCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGActgc < 2:401159/233‑1 (MQ=255) ggTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCAt > 1:477085/1‑236 (MQ=255) ccTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAg < 1:457053/235‑1 (MQ=255) aaTGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGc > 1:4244/1‑236 (MQ=255) gcgGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCg < 2:158059/211‑1 (MQ=255) gcgGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCg > 1:158059/1‑211 (MQ=255) ggCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAg < 2:4244/235‑1 (MQ=255) ggCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAg < 1:11568/235‑1 (MQ=255) cGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCa < 1:759726/236‑1 (MQ=255) cacaTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGttt > 1:283625/1‑181 (MQ=255) cacaTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGttt < 2:283625/181‑1 (MQ=255) acaTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGAcgcg < 1:49970/236‑1 (MQ=255) tCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAg > 1:213576/1‑236 (MQ=255) cATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCg < 1:215001/167‑1 (MQ=255) cATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCg > 2:215001/1‑167 (MQ=255) atagCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAgg > 1:436947/1‑228 (MQ=255) atagCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAgg < 2:436947/228‑1 (MQ=255) cagcCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCt > 1:121170/1‑235 (MQ=255) agcCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGa < 2:509538/197‑1 (MQ=255) agcCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGa > 1:509538/1‑197 (MQ=255) ctttgcGGACTGTAAGTCGATGTG‑TCGAGGCGGCGGTTGC‑GGGCGGTGATCCGGGTTCCCCGGGGT‑GTCGAGCCCGCCTTCCGCTGAGGGGAGTTTGCGCTAAAGCACCATCAAGCTGGGTGTGTGGCGGTTTAAGTCGGCGTTTAAGCCCTGGCGACTGCGGGCATTGAGCCCGCCGAGCGGGGTTCCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCg < 2:217722/231‑1 (MQ=255) cGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGCGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCTGCCCGACACGCAGGTTGGTTTCGCGACAGCCACAACACCACAGCGTCgtg > 2:667956/1‑234 (MQ=255) cGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGt > 1:217722/1‑232 (MQ=255) aacgcGAAGTGCTCGAGTGGGC‑GTCGCAGCCCGTGGATCC‑GG‑TGCCCAAGGTGGCCGGGCGCTCCTTCGGAGGGGGAGGTTTTGCGCGAAAGCACCATCAAGCTGGTGGTGTGGGGGGTTAAGGCGGGGTTTAAGCCCGGGGGACGGCTGGCATTGGGCACGCCGGGCGGGGTTTCCAGCCCGACGCGAAGGTTGGTTTCGCTCCCGCCAAAAACCGCCAGCGTCGAGCTGGGGtt < 2:693300/232‑1 (MQ=255) cGACGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCG‑GGTTGCCCAGGGT‑GCCGAGCGCCCTTGTCGCGGAGGAGAGTTTGCGCTAAAGCACAATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCGGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGTTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCat < 2:121170/235‑1 (MQ=255) aGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCGGACGCGCAGGTTGGCTTCGCTACCGCCAACAACCGCCAGCGCCGAGCTGCGGTGCCTATCa > 2:598337/1‑236 (MQ=255) tgtTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGActgc > 2:426035/1‑139 (MQ=255) tgtTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGActgc < 1:426035/139‑1 (MQ=255) c‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTtgag > 1:654481/1‑236 (MQ=255) gCCCAGGGT‑GTGGAGCGCGCTGTGCGCTGAGGAGAGTTTGCGCTAAAGCCCCATCAAGCGGGGTGTGTGGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATGGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGTTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaata < 2:474395/202‑1 (MQ=255) gt‑gtCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGt > 1:474395/1‑202 (MQ=255) gcCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAg > 1:430476/1‑236 (MQ=255) cAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTg > 1:453291/1‑231 (MQ=255) gggTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCg < 1:725296/185‑1 (MQ=255) gggTTAAGTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCg > 2:725296/1‑185 (MQ=255) | GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGC‑GTTGCAGGCCGTTGATCC‑GGTTGCCCAGAGT‑GTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTG > NC_000913/1192148‑1192576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |