Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,256,574 T→G intergenic (‑622/+147) ycgV ← / ← ychF putative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,256,5740TG98.1% 185.4 / NA 53intergenic (‑622/+147)ycgV/ychFputative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (27/25);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGC  >  NC_000913/1256344‑1256807
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
taaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACta                                                                                                                                                                                                                                      <  2:351149/236‑1 (MQ=255)
                        atTCATTTGTGGTCAGTGGGTTTTCTGGCCTATCGTTGTTATTGTGCGACGGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAgtgt                                                                                                                                                                                                                <  2:202431/234‑1 (MQ=255)
                         ttCATTGTTGGTCAATGGGTTTTCTGGCCTAGCGTTGGTATTGTGCTACGGGTTAGCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAgg                                                                                                                                                                                                             <  2:502358/236‑1 (MQ=255)
                          tCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGa                                                                                                                                                                                                            <  1:61174/236‑1 (MQ=255)
                                   gTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACaa                                                                                                                                                                                                                      >  2:458012/1‑217 (MQ=255)
                                   gTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACaa                                                                                                                                                                                                                      <  1:458012/217‑1 (MQ=255)
                                                  ggCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCa                                                                                                                                                                                                                                         >  2:434339/1‑183 (MQ=255)
                                                  ggCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCa                                                                                                                                                                                                                                         <  1:434339/183‑1 (MQ=255)
                                                                                 cATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGa                                                                                                                                                     <  2:25191/236‑1 (MQ=255)
                                                                                 cATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATACCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCg                                                                                                                                                         <  2:645077/232‑1 (MQ=255)
                                                                                 cATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATACCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCg                                                                                                                                                         >  1:645077/1‑232 (MQ=255)
                                                                                        aTCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTtata                                                                                                                                              <  2:443742/236‑1 (MQ=255)
                                                                                                            cGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGAt                                                                                                                                                                                             >  2:581230/1‑169 (MQ=255)
                                                                                                            cGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGAt                                                                                                                                                                                             <  1:581230/169‑1 (MQ=255)
                                                                                                            cGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTgg                                                                                                                                                      <  2:331901/208‑1 (MQ=255)
                                                                                                            cGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTgg                                                                                                                                                      >  1:331901/1‑208 (MQ=255)
                                                                                                            cGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATga                                                                                                                          >  1:90647/1‑236 (MQ=255)
                                                                                                              tGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATgaga                                                                                                                        >  1:229414/1‑236 (MQ=255)
                                                                                                               gATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGAt                                                                                                                       >  1:546266/1‑236 (MQ=255)
                                                                                                                      tttGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGa                                                                                                                <  2:546266/236‑1 (MQ=255)
                                                                                                                      tttGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGa                                                                                                                <  2:128203/236‑1 (MQ=255)
                                                                                                                                         ggCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATcc                                                                                                                                                                    <  1:723946/165‑1 (MQ=255)
                                                                                                                                         ggCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATcc                                                                                                                                                                    >  2:723946/1‑165 (MQ=255)
                                                                                                                                          gCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAACACTCATGAGACAATAaat                                                                                            >  2:701852/1‑236 (MQ=255)
                                                                                                                                            tGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaatta                                                                                          >  1:175007/1‑236 (MQ=255)
                                                                                                                                            tGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCAGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaatta                                                                                          >  1:481872/1‑236 (MQ=255)
                                                                                                                                                   tAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGAc                                                                                   >  1:440141/1‑236 (MQ=255)
                                                                                                                                                        aTACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGtga                                                                                                                                                                                <  1:246444/138‑1 (MQ=255)
                                                                                                                                                        aTACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGtga                                                                                                                                                                                >  2:246444/1‑138 (MQ=255)
                                                                                                                                                        aTACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGCAACCTCATGAGACAATAAATTAATTTGACGTTGa                                                                              >  2:592649/1‑236 (MQ=255)
                                                                                                                                                        aTACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTACGTGGATTTTATATACTTTTGCGCTATTAATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGa                                                                              >  2:183678/1‑236 (MQ=255)
                                                                                                                                                           cAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGcaca                                                                                                                                                                                                                       >  1:547805/1‑96 (MQ=255)
                                                                                                                                                           cAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGcaca                                                                                                                                                                                                                       <  2:547805/96‑1 (MQ=255)
                                                                                                                                                           cAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGCATGATTAGGACTCGGGGAAATGAAAAATCCACGCAATTGCGCGGATTTTATATACTTTTTCGCTCTTCATGAGAATTATCGACAACTCCTTAGACAAAACATTACATAGAAGTTGaaaa                                                                           >  2:389805/1‑236 (MQ=255)
                                                                                                                                                           cAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGct                                                                                                                                  <  1:156091/181‑1 (MQ=255)
                                                                                                                                                           cAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGct                                                                                                                                  >  2:156091/1‑181 (MQ=255)
                                                                                                                                                             aaaaCTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAgg                                                                         <  1:304246/236‑1 (MQ=255)
                                                                                                                                                                              aCTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGcc                                                        <  2:175007/236‑1 (MQ=255)
                                                                                                                                                                              aCTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGcc                                                        <  2:229414/236‑1 (MQ=255)
                                                                                                                                                                                tGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACaa                                                                                                                                                                                                                      <  2:337260/76‑1 (MQ=255)
                                                                                                                                                                                tGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACaa                                                                                                                                                                                                                      >  1:337260/1‑76 (MQ=255)
                                                                                                                                                                                  cTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCt                                                    >  1:495526/1‑236 (MQ=255)
                                                                                                                                                                                          atatCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGAt                                            >  2:436544/1‑236 (MQ=255)
                                                                                                                                                                                          atatCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGAt                                            >  2:531062/1‑236 (MQ=255)
                                                                                                                                                                                            atCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTCATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATg                                           >  1:728390/1‑235 (MQ=255)
                                                                                                                                                                                            atCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCa                                                >  2:468504/1‑230 (MQ=255)
                                                                                                                                                                                            atCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCa                                                <  1:468504/230‑1 (MQ=255)
                                                                                                                                                                                              ccGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGAt                                            <  2:90647/232‑1 (MQ=255)
                                                                                                                                                                                                  cATCTGAACACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATc                                      >  1:458307/1‑234 (MQ=255)
                                                                                                                                                                                                        aaCACTTATGTCTTTAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCtt                              <  1:436544/236‑1 (MQ=255)
                                                                                                                                                                                                                     ttAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACtt                                                                                                                                         <  2:375630/116‑1 (MQ=255)
                                                                                                                                                                                                                     ttAATTATTCTCGTGGTGCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACtt                                                                                                                                         >  1:375630/1‑116 (MQ=255)
                                                                                                                                                                                                                                    gTTCCCTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATTCTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTcgc  <  2:753141/236‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
TAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGC  >  NC_000913/1256344‑1256807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑