Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,461,931	A→C	N347H (AAC→CAC)	paaJ →	3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,461,931	0	A	C	100.0%	227.0 / NA	75	N347H (AAC→CAC)	paaJ	3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/38);  total (37/38)

CGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGC  >  NC_000913/1461727‑1462162                                                                                                                                                                                                              |                                                                                                                                                                                                                                         cgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGGGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCa                                                                                                                                                                                                           >  2:517612/1‑236 (MQ=255) cgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGGGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCGGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGGGCCATCGCCTTAGGCCa                                                                                                                                                                                                           >  1:669816/1‑236 (MQ=255)         tATCGTGGCCGTGCCAACCGCCGGGGTGGACCCGCGCCTGATGGGGCTTGGTCCGGTGCCGGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTg                                                                                                                                                                                                    <  2:386697/235‑1 (MQ=255)                  cATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGGGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTCGGGAATGAgt                                                                                                                                                                                           >  1:355401/1‑235 (MQ=255)                     ggCACCCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAgtggtg                                                                                                                                                                                       <  1:392713/236‑1 (MQ=255)                     ggCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCCTTGGGatatactggg                                                                                                                                                                                        >  2:2217/1‑226 (MQ=255)                                           cgcCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGGGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCGATCGCCTTAGGCCATCCGTTGGGACTGAGTGCTGGCCGCCGTGCACTGGCTgccag                                                                                                                                                                 >  2:19199/1‑236 (MQ=255)                                                gATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGTAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGTCCCCCTGGGCCTGGCTgcccgcca                                                                                                                                                              >  2:332444/1‑234 (MQ=255)                                                gATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTgccagccc                                                                                                                                                              >  1:151817/1‑233 (MQ=255)                                                  tGGCGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAg                                                                                                                                                          <  2:377499/236‑1 (MQ=255)                                                          ggTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCg                                                                                                                                                  >  1:758138/1‑236 (MQ=255)                                                                gTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAg                                                                                                                                                                                                                              >  1:514750/1‑154 (MQ=255)                                                                gTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAg                                                                                                                                                                                                                              <  2:514750/154‑1 (MQ=255)                                                                    cTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACgg                                                                                                                                         <  2:447824/235‑1 (MQ=255)                                                                    cTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACgg                                                                                                                                         <  1:710053/235‑1 (MQ=255)                                                                           tCGCCGGGTGCTGTAACGCGCAGGGGTG‑CATATTCAGGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGGTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTGAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                  <  2:509015/235‑1 (MQ=255)                                                                                 ggTGCAGGACCGTGCAGGGATGCAGTCTACA‑GATTTGGGCGTGATTGGACTGAACGAAGCGTTCGGGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCa                                                                                                                               <  2:722474/232‑1 (MQ=255)                                                                                       ggAACGCGCAGGGTTG‑AGTATTCACGATATGGACTTGATTGACCTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑cc                                                                                                                     <  2:39473/235‑1 (MQ=255)                                                                                       ggAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑cc                                                                                                                     <  1:2217/235‑1 (MQ=255)                                                                                         accgcgAAGGGCTG‑AGTATTCACGATAGGGACGTGATTGAACGGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCAtg                                                                                                                  <  2:760356/234‑1 (MQ=255)                                                                                             cgcAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCAt                                                                                                                                                                                                          >  2:298282/1‑145 (MQ=255)                                                                                             cgcAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCAt                                                                                                                                                                                                          <  1:298282/145‑1 (MQ=255)                                                                                             cgcAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCACGTGCa                                                                                                              >  2:47218/1‑236 (MQ=255)                                                                                                       gggTATTCACGATATGGACGTGATTGACCTGAACGAAGCGTTCGCGGCCAAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCATGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGgt                                                                                                         <  2:669816/230‑1 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                  >  2:686581/1‑202 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                  <  1:686581/202‑1 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCCATG‑GCATCGGTGTCCGTCatg                                                                                               >  2:115341/1‑234 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAgg                                                                                               >  2:544189/1‑236 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAgg                                                                                               >  2:629398/1‑236 (MQ=255)                                                                                                             tCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAgg                                                                                               >  1:597299/1‑236 (MQ=255)                                                                                                               aCGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGc                                                                                             >  1:214203/1‑236 (MQ=255)                                                                                                                    atGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAACTGCATCTGCGTAACGGTCGTTACGCATTGTGCA‑CCAGTGGCATCGGGGTCGGTCAGGGCATGGc                                                                                        >  2:421851/1‑236 (MQ=255)                                                                                                                       gACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                      <  1:517612/235‑1 (MQ=255)                                                                                                                       gACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                      <  2:758138/235‑1 (MQ=255)                                                                                                                             aTTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTgccagcca                                                                                                                                                              >  2:100064/1‑158 (MQ=255)                                                                                                                             aTTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTgccagcca                                                                                                                                                              <  1:100064/158‑1 (MQ=255)                                                                                                                             aTTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATTGCCATGATTCt                                                                               >  1:27281/1‑236 (MQ=255)                                                                                                                               tgaactgaacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCAt                                                                                                                              <  2:539097/188‑1 (MQ=255)                                                                                                                               tgaactgaacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCAt                                                                                                                              >  1:539097/1‑188 (MQ=255)                                                                                                                                 aactgaacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                           >  1:171484/1‑236 (MQ=255)                                                                                                                                 aactgaacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                           <  2:597299/236‑1 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCAt                                                                                                                                                    <  1:199668/159‑1 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCAt                                                                                                                                                    >  2:199668/1‑159 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCa                                                                                                                        <  1:690127/187‑1 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCa                                                                                                                        >  2:690127/1‑187 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCa                                                                                                 <  2:481862/209‑1 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCa                                                                                                 >  1:481862/1‑209 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                      >  1:233474/1‑236 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                      >  1:122859/1‑236 (MQ=255)                                                                                                                                      aacGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGACCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCCTCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                      >  1:527959/1‑236 (MQ=255)                                                                                                                                           aGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAACGCGATTGAg                                                                 >  2:622888/1‑236 (MQ=255)                                                                                                                                                 cgcgGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCatat                                                            <  1:421851/235‑1 (MQ=255)                                                                                                                                                             gTTGGGTGTACTCCGCGACTTGGGGCTGCCTTATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                               <  2:355401/236‑1 (MQ=255)                                                                                                                                                               tGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGa                                                  <  1:629398/231‑1 (MQ=255)                                                                                                                                                               tGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                 <  1:544189/232‑1 (MQ=255)                                                                                                                                                               tGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                               <  1:240386/234‑1 (MQ=255)                                                                                                                                                               tGGGTGTACTACGCGAATTGGGCCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                    <  1:332444/229‑1 (MQ=255)                                                                                                                                                                   tgtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCTGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTTCGCGTAcctttc                                         >  2:462148/1‑232 (MQ=255)                                                                                                                                                                     tactacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                      >  1:188105/1‑205 (MQ=255)                                                                                                                                                                     tactacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                      <  2:188105/205‑1 (MQ=255)                                                                                                                                                                        tacGCGATGTGGGGCGGCCGGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                               <  2:735861/225‑1 (MQ=255)                                                                                                                                                                        tacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATg                                     <  1:622888/235‑1 (MQ=255)                                                                                                                                                                           gcgAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCAt                                                                                                             <  1:304916/160‑1 (MQ=255)                                                                                                                                                                           gcgAATTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCAt                                                                                                             >  2:304916/1‑160 (MQ=255)                                                                                                                                                                               aTTGGGGCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGCATACCCTACa                                        >  1:735861/1‑225 (MQ=255)                                                                                                                                                                                    ggCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATAc                          >  1:443629/1‑234 (MQ=255)                                                                                                                                                                                    ggCTGCCTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACaa                        <  1:646281/236‑1 (MQ=255)                                                                                                                                                                                         ccTGATGATGCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCt                    <  1:462148/235‑1 (MQ=255)                                                                                                                                                                                             atgatgCCCCACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAcc               <  2:233474/236‑1 (MQ=255)                                                                                                                                                                                                   ccccACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                  <  1:749468/116‑1 (MQ=255)                                                                                                                                                                                                   ccccACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                  >  2:749468/1‑116 (MQ=255)                                                                                                                                                                                                   ccccACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATg                                     >  2:622189/1‑208 (MQ=255)                                                                                                                                                                                                   ccccACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATg                                     <  1:622189/208‑1 (MQ=255)                                                                                                                                                                                                   ccccACATGTTCACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGGACCCTACCATGATAACCAATACAAAGCTTGACCCGAtt          >  2:407370/1‑235 (MQ=255)                                                                                                                                                                                                          tGTTCACCCCAACGGAGGCGGTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgc  <  2:151817/236‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                         CGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTG‑AGTATTCACGATATGGACGTGATTGAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCA‑CCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGC  >  NC_000913/1461727‑1462162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑