Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,936	A→C	N267H (AAC→CAC)	ynbC →	putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,936	0	A	C	98.7%	269.9 / ‑6.4	76	N267H (AAC→CAC)	ynbC	putative esterase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (36/39);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGA  >  NC_000913/1479703‑1480167                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        aTCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAAc                                                                                                                                                                                                                                       >  1:206589/1‑236 (MQ=255)  tCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGcaca                                                                                                                                                                                                                                      <  1:639565/236‑1 (MQ=255)    cGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAg                                                                                                                                                                                                                                     >  2:45043/1‑235 (MQ=255)      cTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAg                                                                                                                                                                                                                                     >  1:597791/1‑233 (MQ=255)       tGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcg                                                                                                                                                                                                                                 <  2:73787/236‑1 (MQ=255)              acGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAt                                                                                                                                                                                                                          >  2:5560/1‑236 (MQ=255)                gggCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAttt                                                                                                                                                                                                                        >  1:48676/1‑236 (MQ=255)                           gTTAATCTCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             <  2:388631/236‑1 (MQ=255)                           gTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             <  1:464855/236‑1 (MQ=255)                             tAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaa                                                                                                                                                                                                            <  1:45043/235‑1 (MQ=255)                                   cTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTtta                                                                                                                                                                                                     >  1:97527/1‑236 (MQ=255)                                    ttGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTttat                                                                                                                                                                                                    >  1:109262/1‑236 (MQ=255)                                      gCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             <  1:681831/225‑1 (MQ=255)                                      gCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             >  2:681831/1‑225 (MQ=255)                                       cTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAg                                                                                                                                                                                                 <  2:454383/236‑1 (MQ=255)                                            tctcTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGtt                                                                                                                                                                                            <  2:48676/236‑1 (MQ=255)                                                tACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTtata                                                                                                                                                                                        <  1:764570/236‑1 (MQ=255)                                                 aCAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGCCGTTTatat                                                                                                                                                                                       >  1:579847/1‑236 (MQ=255)                                                     aaCGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGTTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                                                                                                                                                                   >  1:436410/1‑236 (MQ=255)                                                     aaCGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                                                                                                                                                                   >  1:485292/1‑236 (MQ=255)                                                          cTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGt                                                                                                                                                                                                <  2:256595/218‑1 (MQ=255)                                                          cTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGt                                                                                                                                                                                                >  1:256595/1‑218 (MQ=255)                                                                       taGAGTTGAGGGCGCGATTACGTTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                                                                                                                                                                 <  2:394918/236‑1 (MQ=255)                                                                           gaTGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTa                                                                                                                                                             >  1:407892/1‑236 (MQ=255)                                                                            aTGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACCAATCGCAACAATTTGATTa                                                                                                                                                             >  1:439817/1‑235 (MQ=255)                                                                            aTGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTa                                                                                                                                                             >  1:265329/1‑235 (MQ=255)                                                                              gcggcggcgATTACGCTCCCCACGCATCTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                                                                                                                                                           <  1:390637/235‑1 (MQ=255)                                                                              gcggcggcgATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             >  2:591680/1‑185 (MQ=255)                                                                              gcggcggcgATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             <  1:591680/185‑1 (MQ=255)                                                                              gcggcggcgATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                                                                                                                                                           <  2:206589/235‑1 (MQ=255)                                                                                ggcggcgATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGc                                                                                                                                                        >  2:766322/1‑236 (MQ=255)                                                                                   ggcgATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCAt                                                                                                                                                      <  1:602807/235‑1 (MQ=255)                                                                                        ttACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGa                                                                                                                                                 <  1:5560/235‑1 (MQ=255)                                                                                        ttACGATCCCCACGAAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGa                                                                                                                                                 <  1:386552/235‑1 (MQ=255)                                                                                           cGCTCCCCACGCATCTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTg                                                                                                                                              <  2:254422/235‑1 (MQ=255)                                                                                              tCCCAACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGAATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca                                                                                                                                          <  2:453669/236‑1 (MQ=255)                                                                                                cccACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             >  1:229742/1‑167 (MQ=255)                                                                                                cccACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                                                                                                                                                             <  2:229742/167‑1 (MQ=255)                                                                                                               atatCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCg                                                                                                                         <  1:766322/236‑1 (MQ=255)                                                                                                                         aTGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATggcg                                                                                                                   >  1:726385/1‑232 (MQ=255)                                                                                                                         aTGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTa                                                                                                               >  2:513182/1‑236 (MQ=255)                                                                                                                                tgtgGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGca                                                                                                                                            <  2:724226/200‑1 (MQ=255)                                                                                                                                tgtgGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGca                                                                                                                                            >  1:724226/1‑200 (MQ=255)                                                                                                                                     tgCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca                                                                                                                                          >  2:627584/1‑197 (MQ=255)                                                                                                                                     tgCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca                                                                                                                                          <  1:627584/197‑1 (MQ=255)                                                                                                                                      gCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGAc                                                                                                  <  2:726385/236‑1 (MQ=255)                                                                                                                                       cATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATggcgg                                                                                                                  >  1:469877/1‑219 (MQ=255)                                                                                                                                       cATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATggcgg                                                                                                                  <  2:469877/219‑1 (MQ=255)                                                                                                                                          cGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCg                                                                                               <  2:109262/235‑1 (MQ=255)                                                                                                                                          cGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGt                                                                                              >  2:275766/1‑236 (MQ=255)                                                                                                                                               aCCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCa                                                                                          >  2:265074/1‑235 (MQ=255)                                                                                                                                                    agtttgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCg                                                                                    <  2:97527/233‑1 (MQ=255)                                                                                                                                                    aGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCg                                                                                    <  1:234338/236‑1 (MQ=255)                                                                                                                                                      attgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCCGCTGCCAGGCTTTTATCACGACCCGTTGGGTGAAGAGCACAGGGCGCTTGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAATAATTTGATTATCATCATGAAGACTGCACAGGACCATCAACGGATCGATGTCGGCTACGTTCTCGCGGACACCTGCCCTTTACGcc                                                                                  >  2:571874/1‑236 (MQ=255)                                                                                                                                                                aTCCGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAGAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTa                                                                        <  2:513295/236‑1 (MQ=255)                                                                                                                                                                aTCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTa                                                                        >  2:45256/1‑236 (MQ=255)                                                                                                                                                                         tACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                                   <  1:265074/232‑1 (MQ=255)                                                                                                                                                                           cGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTa                                                                        <  2:452276/225‑1 (MQ=255)                                                                                                                                                                           cGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTa                                                                        >  1:452276/1‑225 (MQ=255)                                                                                                                                                                                cccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTACCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCACCGAATCGATGGCGGTCACTTTCTGGTGGACCCGTCCCATTATCGCCGGTTGATTAACCGCATCCCTTTAttc                                                        >  2:73363/1‑234 (MQ=255)                                                                                                                                                                                 ccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                                       <  2:597791/236‑1 (MQ=255)                                                                                                                                                                                 ccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                                       >  2:56669/1‑236 (MQ=255)                                                                                                                                                                                     tGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGATCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCgta                                                     >  2:118463/1‑232 (MQ=255)                                                                                                                                                                                     tGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                                   >  1:633605/1‑236 (MQ=255)                                                                                                                                                                                             cTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaa                                           >  1:125062/1‑236 (MQ=255)                                                                                                                                                                                             cTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaa                                           >  1:556023/1‑236 (MQ=255)                                                                                                                                                                                               gCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAt                                         >  2:265184/1‑236 (MQ=255)                                                                                                                                                                                                  tCTGCTTCCAGGCTTTTATCACGTATCGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAGTCTTTATTAGTCGGTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTTCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGt                                      <  2:584738/236‑1 (MQ=255)                                                                                                                                                                                                    tgctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                                    <  1:513182/236‑1 (MQ=255)                                                                                                                                                                                                        gcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                                 >  1:413424/1‑235 (MQ=255)                                                                                                                                                                                                                 ttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAACTGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCa                            <  2:413424/231‑1 (MQ=255)                                                                                                                                                                                                                 ttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACg                              <  1:159903/229‑1 (MQ=255)                                                                                                                                                                                                                 ttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCa                            <  1:265184/231‑1 (MQ=255)                                                                                                                                                                                                                 ttAGCACGACACGTTGGTTGAAGAGCACAGGGCGCTGGCAATTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACg                              <  2:265329/229‑1 (MQ=255)                                                                                                                                                                                                                                  gTGAAGTGCACAGGGCCTTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTAATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGa      <  2:439817/236‑1 (MQ=255)                                                                                                                                                                                                                                      agagCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcac                                                                                                                                           <  1:671834/99‑1 (MQ=255)                                                                                                                                                                                                                                      agagCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcac                                                                                                                                           >  2:671834/1‑99 (MQ=255)                                                                                                                                                                                                                                       gagcACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGa  >  1:13754/1‑235 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        ATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGA  >  NC_000913/1479703‑1480167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑