Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,556	A→C	I1166L (ATC→CTC)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,556	0	A	C	100.0%	175.2 / NA	52	I1166L (ATC→CTC)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/24);  total (28/24)

CCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCA  >  NC_000913/1486328‑1486785                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       ccGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTcaacaa                                                                                                                                                                                                                                 <  2:555318/236‑1 (MQ=255)                   ggACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGAAATTGCGAAGCAGGTCGAGCAAATCCTTACGGCCGGGTTGAATCTCAACAAACGTCTGAAAAGGCGGGt                                                                                                                                                                                                               >  2:543173/1‑236 (MQ=255)                    gACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCggg                                                                                                                                                                                                                <  2:510532/234‑1 (MQ=255)                    gACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCgg                                                                                                                                                                                                                 <  1:550230/233‑1 (MQ=255)                        gTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGat                                                                                                                                                                                                           >  2:755338/1‑235 (MQ=255)                         tACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGata                                                                                                                                                                                                          >  1:97235/1‑235 (MQ=255)                         tACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGGGGata                                                                                                                                                                                                          >  1:541383/1‑235 (MQ=255)                              tAACCCGTATGGCAAAGCGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCa                                                                                                                                                                                                    <  2:491128/236‑1 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACTACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAgg                                                                                                                                                                                                                     >  1:72102/1‑214 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACTACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAgg                                                                                                                                                                                                                     <  2:72102/214‑1 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCgg                                                                                                                                                                                                                 >  2:233068/1‑218 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCgg                                                                                                                                                                                                                 <  1:233068/218‑1 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGatat                                                                                                                                                                                                         <  2:674952/226‑1 (MQ=255)                                   cGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGatat                                                                                                                                                                                                         >  1:674952/1‑226 (MQ=255)                                          cAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGc                                                                                                                                                                                        <  1:294121/236‑1 (MQ=255)                                                         gATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTCAAg                                                                                                                                                                         >  1:359420/1‑236 (MQ=255)                                                         gATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGGCTGAAAGGGCGGGGGGTATTGACCATGGGCCTGGGGCTTTCTGTCCTTaaat                                                                                                                                                                         >  2:676430/1‑235 (MQ=255)                                                                   tGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATggg                                                                                                                                                               <  1:161593/236‑1 (MQ=255)                                                                   tGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATggg                                                                                                                                                               <  1:730067/236‑1 (MQ=255)                                                                               ggTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTcaaca                                                                                                                                                                                                                                  >  2:632491/1‑157 (MQ=255)                                                                               ggTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTcaaca                                                                                                                                                                                                                                  <  1:632491/157‑1 (MQ=255)                                                                                 tgtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGTGTGGGTATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATc                                                                                                                                                 >  2:454829/1‑236 (MQ=255)                                                                                 tgtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATc                                                                                                                                                 >  1:207495/1‑236 (MQ=255)                                                                                  gtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATc                                                                                                                                                 <  1:612456/235‑1 (MQ=255)                                                                                   tgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGCAAGTGCGTATGGATACGACCATGGCGCTGGGGCTTCCTGACCTTAAAAGGACAATGGGCGGGTTGGGATATc                                                                                                                                                 >  2:428442/1‑234 (MQ=255)                                                                                   tgGATAAATTGATCGACGCCAATGGAGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATcgc                                                                                                                                               >  2:300429/1‑236 (MQ=255)                                                                                             gaccgccGCAAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                                                                                                                                     <  2:97235/230‑1 (MQ=255)                                                                                               tCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACt                                                                                                                                   >  2:196763/1‑236 (MQ=255)                                                                                                         tggtggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACgg                                                                                                                           <  1:543173/234‑1 (MQ=255)                                                                                                             ggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGAATATCGCGGTTTTGTCACTGGTAACGGCTTCaa                                                                                                                     >  1:194053/1‑236 (MQ=255)                                                                                                              gCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGGGGGTTGGATAATGGCGGGTTGGGACCTGGTACAGGCTTCaaa                                                                                                                    >  2:247013/1‑236 (MQ=255)                                                                                                              gCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCaa                                                                                                                     >  1:625523/1‑235 (MQ=255)                                                                                                                    tCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGCCATTAAAGCGCCGCTGGGGGGGTTGGTATATACCGGTTTTGTCAATGGTAACGGGTTCAAAAGGCTg                                                                                                              >  2:351498/1‑236 (MQ=255)                                                                                                                              agaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCTCGGTGTTGTCACTGGTAACGGCTTCAAACGGCTgggtggcaagc                                                                                                    >  1:767145/1‑228 (MQ=255)                                                                                                                              agaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGc                                                                                                   >  1:451223/1‑236 (MQ=255)                                                                                                                                 agGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGGTATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTg                                                                                                 <  1:454829/235‑1 (MQ=255)                                                                                                                                    cTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCg                                                                                               <  1:127920/234‑1 (MQ=255)                                                                                                                                                 cATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACgg                                                                                                                 >  2:423630/1‑204 (MQ=255)                                                                                                                                                 cATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACgg                                                                                                                 <  1:423630/204‑1 (MQ=255)                                                                                                                                                 cATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTTGGGGATAGCGC‑GCGAAGATTGCAGGCGAt                                                                                >  2:77268/1‑236 (MQ=255)                                                                                                                                                   tGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTTTGACATTAAAGCGCAGATGGGGGGGTTGGTATATTGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGGGCAA‑CGCTGCGAACTCTGCAGGGGATTg                                                                              >  2:674504/1‑236 (MQ=255)                                                                                                                                                           tgcgtgcCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGAtttacaa                                                                          >  2:532882/1‑227 (MQ=255)                                                                                                                                                             cgtgcCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGGTTTGTCACTGGTAACGGGTTCAAAAGGCTGGGCGACA‑CCGTGGGATACTTTCAGGCGATTGGAAAACGGcc                                                                    >  2:174381/1‑235 (MQ=255)                                                                                                                                                               tgcCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCt                                                                    <  2:416802/234‑1 (MQ=255)                                                                                                                                                                             acacGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGt                                                     <  1:674504/235‑1 (MQ=255)                                                                                                                                                                                     gtggATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                <  1:300429/232‑1 (MQ=255)                                                                                                                                                                                        gATATTGCGAGGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATc                                          >  1:428443/1‑235 (MQ=255)                                                                                                                                                                                            ttGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGa                                      <  1:755338/235‑1 (MQ=255)                                                                                                                                                                                                   gCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTgcg                               <  1:351498/235‑1 (MQ=255)                                                                                                                                                                                                       gTCGAGCAAATCCTTACGGCAGTGTTCAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACG‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCCCATCCCGAcccgggccg                             >  1:35682/1‑226 (MQ=255)                                                                                                                                                                                                                                 cAATCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcagca  <  1:174381/234‑1 (MQ=255)                                                                                                                                                                                                                                  aaTCTCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGa                                      <  1:108600/197‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       CCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACA‑CGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCA  >  NC_000913/1486328‑1486785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑