Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,562,611 A→C F156V (TTT→GTT)  ddpX ← D‑ala‑D‑ala dipeptidase, Zn‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,562,6110AC98.2% 151.3 / ‑4.8 57F156V (TTT→GTT) ddpXD‑ala‑D‑ala dipeptidase, Zn‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (27/29);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

CACCAGCATATCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAAAACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACATCAACAACATATTGCGGGTCTGGGCAGGCTTGCC  >  NC_000913/1562383‑1562838
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   
cACCAGCATATCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAAAACCGcca                                                                                                                                                                                                                              >  1:701981/1‑236 (MQ=255)
 ccaaGCATTTCCTTGTGTGCGGCGGCGTGCGACACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGccac                                                                                                                                                                                                                             <  2:111631/233‑1 (MQ=255)
    aGCATATCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCCTTCGCTGGGGATACCGGCCACACCGCAACCAg                                                                                                                                                                                                                          >  2:382642/1‑236 (MQ=255)
    aGCATATCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGGAATTCGCAGTGCCACCATTCGCTGGAGATACCGACAACACAGCCACCAg                                                                                                                                                                                                                          >  1:710400/1‑236 (MQ=255)
       atatCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGccacca                                                                                                                                                                                                                           <  2:318527/232‑1 (MQ=255)
         atCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCAtt                                                                                                                                                                                                                     >  1:563295/1‑236 (MQ=255)
               tGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGATGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACCTGCTGCCTGGGGGAATTCGAAGCGCACCCAGTTGCTGGGGATACGGGCACCACGGCCACCAGGAATTATAGc                                                                                                                                                                                                                >  2:657805/1‑235 (MQ=255)
                    cggcggCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCa                                                                                                                                                                                                           >  1:245931/1‑235 (MQ=255)
                     ggcggcGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGc                                                                                                                                                                                                         >  2:38686/1‑236 (MQ=255)
                               gcAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGa                                                                                                                                                                                                <  2:563295/235‑1 (MQ=255)
                                             tgtgGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAgcggcg                                                                                                                                                                                 >  1:753034/1‑236 (MQ=255)
                                             tgtgGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAAAAGCCGATTGCGCTGAgcggcg                                                                                                                                                                                 >  1:731902/1‑236 (MQ=255)
                                               ggccAAGGACGTGCGGGAGCAATTGGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCggcggg                                                                                                                                                                               <  1:382642/233‑1 (MQ=255)
                                                 gCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCggcgggcg                                                                                                                                                                             <  1:532397/236‑1 (MQ=255)
                                                                aGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTg                                                                                                                                                                <  1:435013/234‑1 (MQ=255)
                                                                 caaagtggggACGAACCGAATCCAATCTCTTCATGACAGTTTCCGGATTAACTGACGTGCTGGTGGCCAGGGGATAAAAACCAAGAGAATTGACCGGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACCCCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGAt                                                                                                                                                              <  2:305691/228‑1 (MQ=255)
                                                                           aCGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGGCGGGAGGGGGATAGGGCTGGGa                                                                                                                                                    >  2:8571/1‑235 (MQ=255)
                                                                           aCGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAg                                                                                                                                                   <  1:504562/236‑1 (MQ=255)
                                                                               aaCGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACATCCGATTGCGCTGAGCGGCGGGCGGGATGGAAAGGTGATAAGCATGGGAgcgac                                                                                                                                               >  2:289014/1‑234 (MQ=255)
                                                                               aaCGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAACAGGGGAAGGCATGGGAgcgcc                                                                                                                                               >  2:88568/1‑235 (MQ=255)
                                                                                     atCGTTCTCTTCATGACAGTTTCCTGATTAACTGACTGGCTGTGTGCCAGGGAATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTACCTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCa                                                                                                                                         <  2:731902/236‑1 (MQ=255)
                                                                                       cgttctcTGAATGACAGTTTCCTGATTAACTGACGTGCTGGGTGCAAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGGGGGTAACTCGCTGCCGGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCa                                                                                                                                         <  2:36176/231‑1 (MQ=255)
                                                                                         aTCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGGGATAGGCATGGGAGCGCTCGTGCATTTc                                                                                                                                     >  1:467232/1‑236 (MQ=255)
                                                                                           ctctTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAAGGTGATAGGCATGGGAGCGCTCGTGCAGTTCAt                                                                                                                                   >  1:36176/1‑236 (MQ=255)
                                                                                              ttCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGGGCATTTCATCg                                                                                                                                 >  1:64234/1‑235 (MQ=255)
                                                                                                    acctgttCCTGATTAACGGACGGTGTGGGGGCCAGGGGAAAAAAAACAAGAAAATGGATCAGCGAGCAGGGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCCCCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTg                                                                                                                          <  2:753034/231‑1 (MQ=255)
                                                                                                        tttCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCt                                                                                                                                                                                         <  2:505896/169‑1 (MQ=255)
                                                                                                        tttCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCt                                                                                                                                                                                         >  1:505896/1‑169 (MQ=255)
                                                                                                        tttCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACgg                                                                                                                                                                       <  2:698372/187‑1 (MQ=255)
                                                                                                        tttCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACgg                                                                                                                                                                       >  1:698372/1‑187 (MQ=255)
                                                                                                            cgtaTTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGcc                                                                                                                      <  1:479247/229‑1 (MQ=255)
                                                                                                            cTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTgcg                                                                                                                        <  1:38686/230‑1 (MQ=255)
                                                                                                                ttAACGGACGTGCTTGTGGCCGGGGAATAAAAAGCAAGGAAGTGTGTCAGCGAGCGGAGGGAAACTCGCTTCTTGGGGTAATTCGAAGTGCCACCATTCGCGGGAGATACCAAAAGCACCGCCCCCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGa                                                                                                              <  2:245931/236‑1 (MQ=255)
                                                                                                                   acgagcGTGCTGGGGGCAGGGGGATATAAAACAAGAGAATTAGCCAGCGAGCAGGGGGAAACCCGCAGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACCCCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGa                                                                                                           <  2:467232/231‑1 (MQ=255)
                                                                                                                               tgtgCCAGGGGATATAAAACAAGAGAATGGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGc                                                                                                <  1:643573/235‑1 (MQ=255)
                                                                                                                                          gATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGGCGGAAGGGGGATAGGCATGGTAGCGTCCGTGCATCTCATCGAACCCTTCGCCCCATTCGAGGATGTTCCCCTGCtcagtctcta                                                                                      >  2:445874/1‑228 (MQ=255)
                                                                                                                                          gATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCCCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGGGCTCATCACGaa                                                                                      >  1:602924/1‑235 (MQ=255)
                                                                                                                                            tataAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGGGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGCGGATGTTCCCGTGCTCATCACGAAGCg                                                                                   >  1:92189/1‑236 (MQ=255)
                                                                                                                                            tataAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGGGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGACCATATCCAGGATGTTCCCGTGGTCATCACGacacg                                                                                   >  2:474861/1‑232 (MQ=255)
                                                                                                                                               aaaaCAAGAGAATGGATCCGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTc                                                                                 <  1:88568/235‑1 (MQ=255)
                                                                                                                                                      gagaATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACTGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCCCGAAGCGTCAGGTCGa                                                                          >  1:42048/1‑235 (MQ=255)
                                                                                                                                                        gaATTGGACAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCg                                                                       <  1:85644/236‑1 (MQ=255)
                                                                                                                                                               aTCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCAt                                                                                                                                                        <  1:128816/149‑1 (MQ=255)
                                                                                                                                                               aTCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCAt                                                                                                                                                        >  2:128816/1‑149 (MQ=255)
                                                                                                                                                                 cAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGGGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATTGCCCTGcc                                                                >  1:456393/1‑235 (MQ=255)
                                                                                                                                                                      aGCAGAGGGTAACCTGGTGCCTGGGTTAATTCGAAGTTCCCCCATTCGCTGGAGATCCCGACAACCCCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCt                                                          <  1:289014/236‑1 (MQ=255)
                                                                                                                                                                           agGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGGGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGGGGCCCTAATCAGGATGTTCCCCGGCTCATCACGAAACTTCAGGTGCGTAGCCGTTCCACGGCCGTGag                                                     >  2:357534/1‑235 (MQ=255)
                                                                                                                                                                                 aCTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGGGCATTTCATCGAACCCTGCGCCCCTATCGAGGATGTTCCCGTGCTCCTCACGAAACGGACGGTCGAACGCCCGTCCACGGcctggatttgaa                                                >  2:115057/1‑224 (MQ=255)
                                                                                                                                                                                  cTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAGCCCTGCGCCCATATCGAGGAGGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGGGCCACGGCTGGGATTAGAac                                               >  1:668883/1‑235 (MQ=255)
                                                                                                                                                                                        ccatGGCGTAATTCGAAGACACACCATTCGCTGGAGGTCCCGAACACACCGCCCACAGTCATTACCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGt                                        <  2:456393/233‑1 (MQ=255)
                                                                                                                                                                                          cTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATCCCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTc                                       <  1:474861/235‑1 (MQ=255)
                                                                                                                                                                                            ggggTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCg                                                                                                                                 <  1:649965/143‑1 (MQ=255)
                                                                                                                                                                                             gggAAATTCAAAGTCCCACCATTCGCTGGAGATACCGACAACCCCGCCACCGGTCATTAGCGCAGTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACAt                                   <  2:602924/236‑1 (MQ=255)
                                                                                                                                                                                                   ttCGAAGTGCCACCATTCGCTGGAGATACCGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGGAGGGTGATAGGCCTGGGAGCGCTCGTGCATTTCATCCAACCCTg                                                                                                                          >  2:649965/1‑143 (MQ=255)
                                                                                                                                                                                                           gcaaccaTTCGCTGGAGTTCCTGCAAACCCCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACATCAACAACATATTg                      <  2:42048/232‑1 (MQ=255)
                                                                                                                                                                                                               acaTTCGGTGGAAATCCGGACCACCCCGCCACCAGTCATTATCGAATTAAGCAACGGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACATCAACAACATATTGCggg                  <  2:64234/234‑1 (MQ=255)
                                                                                                                                                                                                                               ccGACAACACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACATCAACAACATATTGCGGGTCTGGGCAGGCTTGcc  <  1:115057/235‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   
CACCAGCATATCTTTTGGTACGGCGGCGTGCGCAACCGGGAAATTTGTGGCAAGGACGAGCGCGAGCAATGTGGGACGAAACGATATCGATCTCTTCATGACAGTTTCCTGATTAACTGACGTGCTGTGTGCCAGGGGATATAAAACAAGAGAATTGATCAGCGAGCAGAGGGTAACTCGCTGCCTGGGGTAATTCGAAGTGCCACCATTCGCTGGAGATACCGACAAAACCGCCACCAGTCATTATCGCATTCAGCAACAGCCGATTGCGCTGAGCGGCGGGCGGGACGGAAGGGTGATAGGCATGGGAGCGCTCGTGCATTTCATCGAACCCTGCGCCCATATCGAGGATGTTCCCGTGCTCATCACGAAGCGTCAGGTCGATCGCCGTGCCACGGCTGTGATTAGAACCGACCGTCACATCAACAACATATTGCGGGTCTGGGCAGGCTTGCC  >  NC_000913/1562383‑1562838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: