Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,705	T→G	N639H (AAC→CAC)	yddB ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,705	0	T	G	100.0%	151.8 / NA	45	N639H (AAC→CAC)	yddB	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/24);  total (21/24)

GCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAAT  >  NC_000913/1575475‑1575936                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        gCTGATGTATTGACCATTTATCTTACATAGGATATGGCAAGCTTTACGCGATTCATGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACg                                                                                                                                                                                                                                    <  2:47165/236‑1 (MQ=255)            gcgcATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGccc                                                                                                                                                                                                                         <  1:68674/236‑1 (MQ=255)             cgcATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGTTTTCCTCATAACCATTGTGTACCGACAAGTTGcc                                                                                                                                                                                                                          >  2:175627/1‑234 (MQ=255)             cgcATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGcc                                                                                                                                                                                                                          >  1:153656/1‑234 (MQ=255)             cgcATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGcc                                                                                                                                                                                                                          >  1:251987/1‑234 (MQ=255)             cgcATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGAATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCt                                                                                                                                                                                                                        >  1:42179/1‑236 (MQ=255)               cATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGt                                                                                                                                                                                                                              >  2:719690/1‑228 (MQ=255)                aTTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGc                                                                                                                                                                                                                     <  2:251987/236‑1 (MQ=255)                                   aTGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGAc                                                                                                                                                                                                  >  1:408785/1‑236 (MQ=255)                                    tGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCACACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACa                                                                                                                                                                                                 <  1:175627/236‑1 (MQ=255)                                          cTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                                                                                                                                                                            <  2:153656/235‑1 (MQ=255)                                          cTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                                                                                                                                                                            <  1:478267/235‑1 (MQ=255)                                          cTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                                                                                                                                                                            <  1:719690/235‑1 (MQ=255)                                          cTTGACGCGCTTCTTGCCAGGACAGCGTATTCGCCCACGCTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                                                                                                                                                                            <  2:668757/235‑1 (MQ=255)                                               cggcattcttGCCATGCCAGCGTATTCGCCCACACTAACCCGATCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGt                                                                                                                                                                                       <  2:583812/230‑1 (MQ=255)                                                                   cGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACTATAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGatat                                                                                                                                                                  >  2:454940/1‑236 (MQ=255)                                                                                    aCCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAACGACAATTTGTGGGTTAATATCTACCTGGCGGCTCTGCATGGGTTCGGCCAGt                                                                                                                                                 >  1:539092/1‑236 (MQ=255)                                                                                         cTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTc                                                                                                                                               >  2:736319/1‑233 (MQ=255)                                                                                                                 tttAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa                                                                                                                                                               >  1:224096/1‑193 (MQ=255)                                                                                                                 tttAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa                                                                                                                                                               <  2:224096/193‑1 (MQ=255)                                                                                                                                      ttagtaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTtata                                                                                               <  2:178919/236‑1 (MQ=255)                                                                                                                                              aTCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAGTTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTaa                                                                                       >  1:24203/1‑236 (MQ=255)                                                                                                                                                cTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAGTTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAAtg                                                                                     <  2:24203/236‑1 (MQ=255)                                                                                                                                                   ccACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAAtggtg                                                                                  >  1:236508/1‑236 (MQ=255)                                                                                                                                                   ccACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGCAAtggtg                                                                                  >  1:527628/1‑236 (MQ=255)                                                                                                                                                              cTATCGTAAGAGCCCAGATTACCGTTATAACCCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTcg                                                                        <  2:42179/235‑1 (MQ=255)                                                                                                                                                               tATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTcgcg                                                                      <  1:736319/236‑1 (MQ=255)                                                                                                                                                                           ccAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtgt                                                                                                                                                                                           >  2:702625/1‑107 (MQ=255)                                                                                                                                                                           ccAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtgt                                                                                                                                                                                           <  1:702625/107‑1 (MQ=255)                                                                                                                                                                               aTTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTtata                                                                                               >  1:424865/1‑195 (MQ=255)                                                                                                                                                                               aTTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTtata                                                                                               <  2:424865/195‑1 (MQ=255)                                                                                                                                                                                      ttATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTtata                                                                                               <  2:469584/188‑1 (MQ=255)                                                                                                                                                                                      ttATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTtata                                                                                               >  1:469584/1‑188 (MQ=255)                                                                                                                                                                                             ccaccTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGa                                        >  1:407643/1‑236 (MQ=255)                                                                                                                                                                                                      tATCAACGGTATGGCTCACAGAATAACCATTGTGTAACGACAAGTTGCCCTGGCTCTTGATATAGCTAAAGACAATTTGGGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCt                               <  2:408785/236‑1 (MQ=255)                                                                                                                                                                                                          aCCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCAc                           <  1:454940/236‑1 (MQ=255)                                                                                                                                                                                                          aCCGGTATTGCTCTCCTCATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTGGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCAc                           <  1:636768/236‑1 (MQ=255)                                                                                                                                                                                                                      ctcctcATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAg                >  1:79797/1‑235 (MQ=255)                                                                                                                                                                                                                         ctcATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTg                                                 <  1:269638/199‑1 (MQ=255)                                                                                                                                                                                                                         ctcATAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTg                                                 >  2:269638/1‑199 (MQ=255)                                                                                                                                                                                                                             tAACCATTTGGTAACGAAAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCAcg        <  2:527628/236‑1 (MQ=255)                                                                                                                                                                                                                             tAACCATTGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCAcg        <  2:236508/236‑1 (MQ=255)                                                                                                                                                                                                                                   ttGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCt                                                                    <  2:310877/170‑1 (MQ=255)                                                                                                                                                                                                                                   ttGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCt                                                                    >  1:310877/1‑170 (MQ=255)                                                                                                                                                                                                                                   ttGTGTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACTCGCAAt  >  1:669524/1‑236 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        GCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGTTCGGCCAGTTCAAAACTGAGGCTGAACGAATGCGTTTTGGTTTTGCCATCGTTGTTATATTCAGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAAT  >  NC_000913/1575475‑1575936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑