Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,873 T→G F307V (TTC→GTC)  yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,8730TG98.5% 226.9 / NA 65F307V (TTC→GTC) yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (34/30);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATC  >  NC_000913/1869643‑1870101
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    
acacCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAATTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTa                                                                                                                                                                                                                                  >  2:472597/1‑235 (MQ=255)
 cacCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACt                                                                                                                                                                                                                                >  1:652648/1‑236 (MQ=255)
              cGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGaacccg                                                                                                                                                                                                                    >  1:338519/1‑231 (MQ=255)
               gTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAgg                                                                                                                                                                                                                   >  2:755277/1‑235 (MQ=255)
                        aCTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAACCAGGCGGCACCAtt                                                                                                                                                                                                         >  1:141593/1‑236 (MQ=255)
                        aCTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCAt                                                                                                                                                                                                          >  1:306533/1‑235 (MQ=255)
                                aaGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAg                                                                                                                                                                                                 <  2:652648/236‑1 (MQ=255)
                                   cGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAgcg                                                                                                                                                                                               <  2:222846/235‑1 (MQ=255)
                                     gCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGccc                                                                                                                                                                                            >  1:599015/1‑236 (MQ=255)
                                                   gccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTgatggat                                                                                                                                                                              <  1:584455/236‑1 (MQ=255)
                                                         cAGGGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGACATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAATAGGAGGCACCATTGTTTCAGGTGCCCTGAAACTGATTGATTAGGTa                                                                                                                                                                        >  2:255142/1‑236 (MQ=255)
                                                                ttttgGCCTGATGGACTTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGa                                                                                                                                                                    <  2:306533/232‑1 (MQ=255)
                                                                gTTTGGCGTGATGAACGTTACCGGTTCAATGGATCAATCCTCTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGa                                                                                                                                                                    <  2:392379/233‑1 (MQ=255)
                                                                       cTGATGGACTTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTta                                                                                                                                                          <  2:128231/236‑1 (MQ=255)
                                                                                       gTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTgg                                                                                                                                          <  1:397064/236‑1 (MQ=255)
                                                                                           aaTGGATCAATCCACATAGGATATGGCTAAGCGTTTTTATAGTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGGGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTgg                                                                                                                                          <  2:748547/232‑1 (MQ=255)
                                                                                                  cAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGc                                                                                                                               >  1:88252/1‑236 (MQ=255)
                                                                                                     tCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAggcgg                                                                                                                                                                                                                <  2:645310/152‑1 (MQ=255)
                                                                                                     tCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAggcgg                                                                                                                                                                                                                >  1:645310/1‑152 (MQ=255)
                                                                                                         cTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGca                                                                                                                                                <  1:358542/212‑1 (MQ=255)
                                                                                                         cTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGca                                                                                                                                                >  2:358542/1‑212 (MQ=255)
                                                                                                          tAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACaa                                                                                                                        >  1:5954/1‑235 (MQ=255)
                                                                                                                   ggCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAag                                                                                                                                                                 <  1:238196/185‑1 (MQ=255)
                                                                                                                   ggCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAag                                                                                                                                                                 >  2:238196/1‑185 (MQ=255)
                                                                                                                   ggCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGa                                                                                                                <  2:5954/234‑1 (MQ=255)
                                                                                                                     cTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGc                                                                                                            <  1:472597/236‑1 (MQ=255)
                                                                                                                              tttATATTCTGCTGTATCTGTTCCTAAGCAGAACGTATACGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCg                                                                                                                                                   >  1:467558/1‑188 (MQ=255)
                                                                                                                                    ttCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGTCGATAACTGGGCCGa                                                                                              >  1:594203/1‑235 (MQ=255)
                                                                                                                                       tgctgTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCg                                                                                                           <  2:446063/219‑1 (MQ=255)
                                                                                                                                       tgctgTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCg                                                                                                           >  1:446063/1‑219 (MQ=255)
                                                                                                                                       tgctgTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGa                                                                                           >  2:366494/1‑235 (MQ=255)
                                                                                                                                        gccttaccTGTTCCTCAGCAGAACGTAAAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGAct                                                                                         <  2:295648/229‑1 (MQ=255)
                                                                                                                                          tgTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGActct                                                                                       <  1:366494/236‑1 (MQ=255)
                                                                                                                                            tATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTta                                                                                                                                                          >  1:142816/1‑167 (MQ=255)
                                                                                                                                            tATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTta                                                                                                                                                          <  2:142816/167‑1 (MQ=255)
                                                                                                                                                        agcagAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGa                                                                                                                <  1:727484/197‑1 (MQ=255)
                                                                                                                                                        agcagAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGa                                                                                                                >  2:727484/1‑197 (MQ=255)
                                                                                                                                                          cagAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTATCCGCTTTGCCATGa                                                                         >  1:585365/1‑234 (MQ=255)
                                                                                                                                                            gAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGATGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGa                                                                         >  2:700126/1‑232 (MQ=255)
                                                                                                                                                            gAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGa                                                                                                                >  2:766998/1‑193 (MQ=255)
                                                                                                                                                            gAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGa                                                                                                                <  1:766998/193‑1 (MQ=255)
                                                                                                                                                                    aaGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTgg                                                                                                   <  2:202469/198‑1 (MQ=255)
                                                                                                                                                                    aaGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTgg                                                                                                   >  1:202469/1‑198 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTg                                                                                                                                                                                    <  2:155389/112‑1 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTg                                                                                                                                                                                    >  1:155389/1‑112 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGTCGATGACTCTCCGCTTTGCCATGAAAACCTGTGGAAAAAat                                                        >  2:568660/1‑236 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGCTGACTCTCCGCTTTGCCATGCAATCCTGGCGAACAAat                                                        >  1:438747/1‑236 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATcc                                                                    >  2:95766/1‑224 (MQ=255)
                                                                                                                                                                         cGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATcc                                                                    <  1:95766/224‑1 (MQ=255)
                                                                                                                                                                                     ataCATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCtgttg                                            <  1:755277/236‑1 (MQ=255)
                                                                                                                                                                                          tCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGt                                       <  2:88252/236‑1 (MQ=255)
                                                                                                                                                                                           ccGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCCTTGCCCTGAAATCCCGGCGAAAAAATTATTACCCGTTGTTCGtt                                      >  2:570713/1‑236 (MQ=255)
                                                                                                                                                                                             ggcatcatACCCAGGCGAAAGAAGTCGATGAACATTAGTTTGTCTACTCGCAGGGAACAGGCGGCACGATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGttat                                    <  1:333/234‑1 (MQ=255)
                                                                                                                                                                                              ccATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCTATAACTGGGCCGATGAATCTCCGCTTTGCCTTGAAATCCAGGCGAAACAATTATTACCTGTTGCTCGtgatt                                   >  2:645869/1‑236 (MQ=255)
                                                                                                                                                                                              ccATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGttat                                    >  2:754212/1‑235 (MQ=255)
                                                                                                                                                                                               catcatACCCAGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCtgttg                                            <  2:599015/226‑1 (MQ=255)
                                                                                                                                                                                                         aGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAg                                                                                                                                                                                                 <  2:629963/67‑1 (MQ=255)
                                                                                                                                                                                                         aGGCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAg                                                                                                                                                                                                 >  1:629963/1‑67 (MQ=255)
                                                                                                                                                                                                           gCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGa                      >  2:203948/1‑236 (MQ=255)
                                                                                                                                                                                                           gCGAAAGAAGTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGa                      >  1:519240/1‑236 (MQ=255)
                                                                                                                                                                                                               aagaagTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGaa                     <  1:203948/233‑1 (MQ=255)
                                                                                                                                                                                                                agaagTCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTa                 >  1:237436/1‑236 (MQ=255)
                                                                                                                                                                                                                     tCGATGAACATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCcgt            >  2:35453/1‑236 (MQ=255)
                                                                                                                                                                                                                            aCATGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTACTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGcac     <  2:65960/236‑1 (MQ=255)
                                                                                                                                                                                                                               tGAGTTTGTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATc  <  1:700126/236‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    
ACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATC  >  NC_000913/1869643‑1870101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: