Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,896,050	A→C	N416H (AAC→CAC)	pabB →	aminodeoxychorismate synthase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,896,050	0	A	C	100.0%	228.4 / NA	64	N416H (AAC→CAC)	pabB	aminodeoxychorismate synthase, subunit I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/25);  total (39/25)

TCTGGTCAGCACCATAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTAACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTG  >  NC_000913/1895821‑1896271                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              tcTGGTCAGCACCATAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAAAATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGAc                                                                                                                                                                                                                         >  1:694522/1‑236 (MQ=255)     gTCAGCCCCATACCGGCCCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGAc                                                                                                                                                                                                                         <  2:101499/232‑1 (MQ=255)        aGCACCATAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACcaa                                                                                                                                                                                                                      >  1:686977/1‑232 (MQ=255)             cATAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTtc                                                                                                                                                                                                                 >  1:72740/1‑232 (MQ=255)              aTAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTtctgc                                                                                                                                                                                                              <  2:184208/234‑1 (MQ=255)               tAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGCCTGCCATTCACGGACAAATTTtctgc                                                                                                                                                                                                              >  2:91092/1‑233 (MQ=255)                  cGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTgcg                                                                                                                                                                                                        <  1:91072/236‑1 (MQ=255)                    gcgcAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTgcggg                                                                                                                                                                                                      >  1:16409/1‑236 (MQ=255)                    gcgcAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTgcggg                                                                                                                                                                                                      >  2:585297/1‑236 (MQ=255)                    gcgcAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTgcggg                                                                                                                                                                                                      >  2:445925/1‑236 (MQ=255)                                gAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGt                                                                                                                                                                                          <  1:240401/236‑1 (MQ=255)                                      ttACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCg                                                                                                                                                                                     >  2:763099/1‑235 (MQ=255)                                       tACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGGGGAATTGTCCCCgt                                                                                                                                                                                    >  1:428018/1‑234 (MQ=255)                                          cagacAGCGATCTGCTGCGCTCAGCTTTGCCTGGTGGCTAAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCg                                                                                                                                                                                     <  2:202837/227‑1 (MQ=255)                                          acGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCg                                                                                                                                                                                     <  1:763099/231‑1 (MQ=255)                                           cGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGAGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAAATGTCGCCGATAGcc                                                                                                                                                                               >  2:502783/1‑236 (MQ=255)                                                gCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGAc                                                                                                                                                                                                                         >  1:533967/1‑189 (MQ=255)                                                gCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGAc                                                                                                                                                                                                                         <  2:533967/189‑1 (MQ=255)                                                                                  aaTAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTa                                                                                                                                        >  1:11038/1‑236 (MQ=255)                                                                                       ccGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGt                                                                                                                                   <  1:445925/236‑1 (MQ=255)                                                                                         ggggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCCGGAAGAAGCGGTATATCAGGACACTCTTGATACAGATAATCg                                                                                                                                    >  2:544227/1‑233 (MQ=255)                                                                                         ggggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGaagaag                                                                                                                                                                      <  1:609752/199‑1 (MQ=255)                                                                                         ggggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGaagaag                                                                                                                                                                      >  2:609752/1‑199 (MQ=255)                                                                                          gggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGTTAAAGTTAATCg                                                                                                                                    >  1:320771/1‑232 (MQ=255)                                                                                          gggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCg                                                                                                                                    >  1:543129/1‑232 (MQ=255)                                                                                          gggCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAAAATCAGGAACCTTTTGATAAAGTTAATCg                                                                                                                                    >  2:602787/1‑232 (MQ=255)                                                                                                 gAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGc                                                                                                                         <  2:16409/236‑1 (MQ=255)                                                                                                     gTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATc                                                                                                                                     >  1:485943/1‑220 (MQ=255)                                                                                                     gTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATc                                                                                                                                     <  2:485943/220‑1 (MQ=255)                                                                                                              aaggAATTTATCGACGAACTGGAGCCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGt                                                                                                             <  2:11038/233‑1 (MQ=255)                                                                                                               tGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTaa                                                                                                           >  1:489596/1‑236 (MQ=255)                                                                                                                ggAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGCGAAGTAAg                                                                                                          >  2:679269/1‑236 (MQ=255)                                                                                                                   aaTTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACg                                                                                                       <  2:72740/236‑1 (MQ=255)                                                                                                                       gtCGACTAACTGGACCCGAAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGa                                                                                                   <  2:668020/235‑1 (MQ=255)                                                                                                                       aTCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGa                                                                                                   <  1:679269/236‑1 (MQ=255)                                                                                                                       aTCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAAATTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTgg                                                                                                    >  2:536229/1‑235 (MQ=255)                                                                                                                                     aCCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGACGTAAGAAGTGGAATAACGTAGACt                                                                                       >  2:606119/1‑234 (MQ=255)                                                                                                                                                         ggTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCACCTGGAGAAGTAAGACGTGGAATACCGTATCCTGACGCTTGATGCATTTTTAt                                                                   >  2:234388/1‑234 (MQ=255)                                                                                                                                                          gTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATcgc                                                                >  2:584501/1‑236 (MQ=255)                                                                                                                                                           tGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGCATTTTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGGGAAGAAAGACGTGGAATACCGTAGTCTGACGCTTGATGAaatttttt                                                                   >  2:570294/1‑230 (MQ=255)                                                                                                                                                              ggCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGa                                                                                                   <  1:70686/197‑1 (MQ=255)                                                                                                                                                              ggCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGa                                                                                                   >  2:70686/1‑197 (MQ=255)                                                                                                                                                                cagcaTTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAAACGTGGAATACCGTAGCCTGACGCTTGTGGATTTTTTATCGCGCtt                                                            >  2:737808/1‑234 (MQ=255)                                                                                                                                                                        gCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACtttt                                                   >  1:230177/1‑235 (MQ=255)                                                                                                                                                                                            aaCATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGa                              <  1:585297/236‑1 (MQ=255)                                                                                                                                                                                            aaCATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGa                              <  1:91092/236‑1 (MQ=255)                                                                                                                                                                                                       cAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAatc                   >  2:739926/1‑236 (MQ=255)                                                                                                                                                                                                       cAGTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAat                    <  2:301699/235‑1 (MQ=255)                                                                                                                                                                                                         gTATTACTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGTGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGCAACCCTAAatca                  >  2:708576/1‑235 (MQ=255)                                                                                                                                                                                                              aCTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGt              >  1:521728/1‑234 (MQ=255)                                                                                                                                                                                                              aCTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCa            <  2:543129/236‑1 (MQ=255)                                                                                                                                                                                                               cTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGa                                                                                                                            <  2:704577/123‑1 (MQ=255)                                                                                                                                                                                                               cTATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGa                                                                                                                            >  1:704577/1‑123 (MQ=255)                                                                                                                                                                                                                tATCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAAGCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAgg          <  1:536229/236‑1 (MQ=255)                                                                                                                                                                                                                 aTCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAg           >  2:712224/1‑234 (MQ=255)                                                                                                                                                                                                                  tCCGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAg           >  2:132834/1‑233 (MQ=255)                                                                                                                                                                                                                    cGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTGAACTTTTGCGCCCGCCAATTAACCGGGAAACCCTAAATCATCGTCAGgctgc      >  2:304099/1‑236 (MQ=255)                                                                                                                                                                                                                    cGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGa                              >  1:317142/1‑212 (MQ=255)                                                                                                                                                                                                                    cGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGa                              <  2:317142/212‑1 (MQ=255)                                                                                                                                                                                                                    cGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAg           >  2:145940/1‑231 (MQ=255)                                                                                                                                                                                                                    cGCACGCTGACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAg           >  2:350565/1‑231 (MQ=255)                                                                                                                                                                                                                        cGCTGACTGCCATTCACGGACAAATGTTCTGCTCGGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCtgtg  <  2:596829/236‑1 (MQ=255)                                                                                                                                                                                                                            gACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAgg          <  1:728505/224‑1 (MQ=255)                                                                                                                                                                                                                            gACTGCCATTCACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAgg          >  2:728505/1‑224 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              TCTGGTCAGCACCATAACGGCGCAACTACCAGAACAGTTACACGCCAGCGATCTGCTGCGCGCAGCTTTTCCTGGTGGCTCAATAACCGGGGCTCCGAAAGTACGGGCTATGGAAATTATCGACGAACTGGAACCGCAGCGACGCAATGCCTGGTGCGGCAGCATTGGCTATTTGAGCTTTTGCGGCAACATGGATACCAGTATTACTATCCGCACGCTGACTGCCATTAACGGACAAATTTTCTGCTCTGCGGGCGGTGGAATTGTCGCCGATAGCCAGGAAGAAGCGGAATATCAGGAAACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTG  >  NC_000913/1895821‑1896271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑