Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,008,526	A→C	I84L (ATC→CTC)	yedE →	UPF0394 family sulphur transport domain‑containing inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,008,526	0	A	C	100.0%	163.6 / NA	48	I84L (ATC→CTC)	yedE	UPF0394 family sulphur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/21);  total (27/21)

TTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGATCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGT  >  NC_000913/2008311‑2008757                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         ttAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTtgcg                                                                                                                                                                                                                        >  1:409266/1‑234 (MQ=255)                           gTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCcaa                                                                                                                                                                                            <  1:193373/236‑1 (MQ=255)                                                  tacttactATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCTGCTGCTTTGCCGCAGCGCTGTGGGCCACCAATGTCAAACTGCGACTGCcgc                                                                                                                                                                     >  1:227301/1‑236 (MQ=255)                                                  tacttactATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCcg                                                                                                                                                                      >  2:405537/1‑235 (MQ=255)                                                   acttactATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCcaa                                                                                                                                                                                            <  2:550563/212‑1 (MQ=255)                                                   acttactATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCcaa                                                                                                                                                                                            >  1:550563/1‑212 (MQ=255)                                                       actATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGACGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGc                                                                                                                                                                >  2:28404/1‑236 (MQ=255)                                                            tttGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTa                                                                                                                                                            >  1:380095/1‑235 (MQ=255)                                                            tttGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTCCTAATGCCCTGCAGCCgaa                                                                                                                                                            >  2:592841/1‑233 (MQ=255)                                                              tGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTAt                                                                                                                                                           >  2:618391/1‑234 (MQ=255)                                                                     aCTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCACAAATGTCAAACTGCGAAGGCCGCGCAGCCGTATCCGCATTAt                                                                                                                                                  >  2:581842/1‑236 (MQ=255)                                                                      cTGGCCCCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATg                                                                                                                                                 <  1:581842/236‑1 (MQ=255)                                                                       tGGCCCCTTTTGGGTTGTCACGGGTGAATTTACCCGTTGGGCCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATg                                                                                                                                                 <  2:409266/235‑1 (MQ=255)                                                                          cACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGc                                                                                                                                                                >  1:347093/1‑217 (MQ=255)                                                                          cACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTACTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGc                                                                                                                                                                <  2:347093/217‑1 (MQ=255)                                                                             ctttgaggCTGGCCCGGGTGAATTGACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGcc                                                                                                                                           <  2:90476/229‑1 (MQ=255)                                                                                        tCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGGGCAGCCGTATCGGCATTATGCAGGCCATGATTggcggc                                                                                                                               >  1:182155/1‑236 (MQ=255)                                                                                        tCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTggcggc                                                                                                                               >  2:442786/1‑236 (MQ=255)                                                                                        tCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTg                                                                                                                                    >  1:90476/1‑231 (MQ=255)                                                                                        tCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCACCATTggcggc                                                                                                                               >  1:643603/1‑236 (MQ=255)                                                                                                 aaTTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGACGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCACTATCGc                                                                                                                       <  1:28404/235‑1 (MQ=255)                                                                                                  aTTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCAGTATCTCCg                                                                                                                     >  2:143778/1‑236 (MQ=255)                                                                                                                            tgaaaCTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATggg                                                                                           <  1:591241/232‑1 (MQ=255)                                                                                                                            tGCATCTGGTCGGCTGCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATGATTGGCGGCATTATCGCCGGTCTTGGCGCGCGTC‑TGGCAATg                                                                                             <  2:182155/233‑1 (MQ=255)                                                                                                                             gCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGc                                                                                          >  1:400455/1‑235 (MQ=255)                                                                                                                              cAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTg                                                                                        <  2:380095/236‑1 (MQ=255)                                                                                                                                     tCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTTGCGCGCGTC‑TTGCAATTGGCTGCAACCTg                                                                                 >  2:405966/1‑236 (MQ=255)                                                                                                                                       agCGTCCATGCTAAAGAGGTGGGTTACTTTAAAATTAGCCATCTGGAAGGATCGCCATTACCCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCa                                                                                                <  2:299808/218‑1 (MQ=255)                                                                                                                                        gCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGTTCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcg                                                                              <  1:421078/236‑1 (MQ=255)                                                                                                                                        gCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcg                                                                              <  1:618391/236‑1 (MQ=255)                                                                                                                                         cGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcgg                                                                             >  1:332060/1‑236 (MQ=255)                                                                                                                                         cGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcg                                                                              <  1:417463/235‑1 (MQ=255)                                                                                                                                              aTGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCGGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAACGGGCt                                                                                         >  1:299808/1‑219 (MQ=255)                                                                                                                                                         tGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGTGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGC‑CGCGGCTTGGCAAGTGGCGGTAACCTGCCGGCGTCCTTTACCGGt                                                               >  2:710307/1‑234 (MQ=255)                                                                                                                                                         tGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGGAACCTGGCTGCGTTCTTTACCgtgc                                                              >  2:687313/1‑232 (MQ=255)                                                                                                                                                             tttACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTcc                                                          <  1:687313/234‑1 (MQ=255)                                                                                                                                                             gTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTc                                                           <  1:405537/234‑1 (MQ=255)                                                                                                                                                                aCTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGTCGGCGTTCTTTACCGGGATTACTCa                                                       >  2:434996/1‑235 (MQ=255)                                                                                                                                                                               aTCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGcc                                       <  1:442786/236‑1 (MQ=255)                                                                                                                                                                                gcgggAAGGATCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCt                                      <  1:166245/233‑1 (MQ=255)                                                                                                                                                                                         aTCGCCATTAACCCGCATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCttt                              >  1:506980/1‑235 (MQ=255)                                                                                                                                                                                                   aCCCGCCTCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCCTTATGCAGGCCATCATTGGCGGCATTATCGCCGGCTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGTGGTCTTTACCGGTCTTCCTAAGTTCTCGCTGCATGCCCGGTTCTTTGctcat                        >  2:68657/1‑227 (MQ=255)                                                                                                                                                                                                        cATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcg                                                                              >  1:71952/1‑172 (MQ=255)                                                                                                                                                                                                        cATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTggcg                                                                              <  2:71952/172‑1 (MQ=255)                                                                                                                                                                                                        cATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCa              >  1:127846/1‑236 (MQ=255)                                                                                                                                                                                                        cATCGACGGGATGATGCTCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGGTCTCGCTGCATTCCTGGTTCCTTGCCATCGCCACTGCCa              >  2:125648/1‑236 (MQ=255)                                                                                                                                                                                                         aTCGACGGGATGATCCTCATCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCAt             <  2:81913/236‑1 (MQ=255)                                                                                                                                                                                                               gggATGATGCTGCTGGTTATGTGTGGCGGTTGCTTTGCCGCAGCGCTGGGGGCAAACAATGTCAAACTGCGAATGCCGCGCAGCCGGATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTc       <  2:227301/236‑1 (MQ=255)                                                                                                                                                                                                                     atgctCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGt  >  1:246990/1‑235 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         TTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGCCATTAACCCGCATCGACGGGATGATGATCCTCGGTATGTTTGGCGGCTGCTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTC‑TGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGT  >  NC_000913/2008311‑2008757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑