Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,020,656 T→G N190K (AAT→AAG)  fliM → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,020,6560TG98.0% 178.0 / NA 51N190K (AAT→AAG) fliMflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (29/21);  minor base C (1/0);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTG  >  NC_000913/2020432‑2020882
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                  
gtggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGCAATTTACCAAGATCACCACCTc                                                                                                                                                                                                                         >  1:719005/1‑236 (MQ=255)
gtggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTc                                                                                                                                                                                                                         >  1:666033/1‑236 (MQ=255)
gtggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTc                                                                                                                                                                                                                         >  1:42570/1‑236 (MQ=255)
     gtTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGGGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTACCCGa                                                                                                                                                                                                                    >  2:537360/1‑236 (MQ=255)
       tctgACCGAGTCTGGTGGTTACCGCCGTGGATAACCTGTTTTGCGGAGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGaa                                                                                                                                                                                                                   <  2:553116/235‑1 (MQ=255)
             cGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACAtt                                                                                                                                                                                                            <  2:615952/236‑1 (MQ=255)
                gTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGCAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCAATCTGCCGAACGACATtgtg                                                                                                                                                                                                         >  2:230102/1‑236 (MQ=255)
                gTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATtgtg                                                                                                                                                                                                         >  2:419280/1‑236 (MQ=255)
                      tgtTTAGCGCCGTGGAATACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGAATGTTGAAACTGGCGGTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGGGGTTaa                                                                                                                                                                                                    <  1:446401/235‑1 (MQ=255)
                       gtTTATCGCCGTGGATAACCTGTTGGGCGGCGATGGACGCTTCCCGGCCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAGCTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAaca                                                                                                                                                                                                  <  1:239543/236‑1 (MQ=255)
                          taccggtgTGGAAAACCTGTGTGGCGCCGATGGACGCTCCCCGACCAAAGGGGAAGGGCGCGAGTTTCCCCAACCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACg                                                                                                                                                                                                <  2:719005/228‑1 (MQ=255)
                             cGCCGGGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGt                                                                                                                                                                                            <  1:100805/236‑1 (MQ=255)
                                cGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTcc                                                                                                                                                                                         >  2:206489/1‑236 (MQ=255)
                                cGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTcc                                                                                                                                                                                         >  1:505453/1‑236 (MQ=255)
                                  tGGATAACCTGTTGGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAAATGGCGCTTGAAGGCCATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTATGTGCGTTCGGAAATGCAGTGTAAATTTCACAACATCACACACTCGCTGAACGAAATATAGGTTCACACCGCGTGCCAt                                                                                                                                                                                       >  2:470481/1‑236 (MQ=255)
                                                   ggcgATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGCAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACAAGCCGTTCCCTTCGGAGATTGGTAAct                                                                                                                                                                       >  2:401654/1‑234 (MQ=255)
                                                    gcgATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTg                                                                                                                                                                     >  1:179959/1‑236 (MQ=255)
                                                           aCGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGc                                                                                                                                                               <  1:419280/235‑1 (MQ=255)
                                                           aCGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGc                                                                                                                                                               <  1:206489/235‑1 (MQ=255)
                                                           aCGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCg                                                                                                                                                              <  1:110203/236‑1 (MQ=255)
                                                                         aaGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGa                                                                                                                                                                    >  1:13555/1‑216 (MQ=255)
                                                                         aaGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGa                                                                                                                                                                    <  2:13555/216‑1 (MQ=255)
                                                                                aGGTCGCGATTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCa                                                                                                                                          <  1:763385/235‑1 (MQ=255)
                                                                                aGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCa                                                                                                                                          <  1:537360/235‑1 (MQ=255)
                                                                                 ggTCGCGAGTTTCCCCATACCGGACAGCCCGTCATCAGCCGCTTTGTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACTACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCa                                                                                                                                          <  2:20840/234‑1 (MQ=255)
                                                                                                cATACGGAGCGGCGCGTCATCAACCGCATGTTGAACCTGGCGCTGGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTGGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGcc                                                                                                                         <  2:42570/236‑1 (MQ=255)
                                                                                                  tACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGc                                                                                                                       >  1:10056/1‑236 (MQ=255)
                                                                                                   aCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACg                                                                                                                                                                                                                 >  2:550041/1‑145 (MQ=255)
                                                                                                   aCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACg                                                                                                                                                                                                                 <  1:550041/145‑1 (MQ=255)
                                                                                                   aCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCt                                                                                                                      >  1:6820/1‑236 (MQ=255)
                                                                                                   aCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCt                                                                                                                      >  1:505861/1‑236 (MQ=255)
                                                                                                   aCCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCt                                                                                                                      >  1:450798/1‑236 (MQ=255)
                                                                                                    ccgaaacgagcgTCACCAACCGAATTTTGAAACTGGCGTTTGAAGGCTAAGGCGCCGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGGCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCCCCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTa                                                                                                                     <  2:10056/227‑1 (MQ=255)
                                                                                                    ccGAACAGCGCGTCATCAACCGCATGTTGAAGCTGGCGCTTGAAGGCTATAGCGACGCCGGGAAGGCGATTAATCCGCTGGAAGTGGAGTACGGGCGTTCGAAAATGCAGGTGAAATTTACCAAGATCACCACCTCTCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTa                                                                                                                     <  1:230102/236‑1 (MQ=255)
                                                                                                       aaCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTaa                                                                                                                                                       >  2:500610/1‑199 (MQ=255)
                                                                                                       aaCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTaa                                                                                                                                                       <  1:500610/199‑1 (MQ=255)
                                                                                                       aaCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAAAACGCCGTTCCAGGTGGAGATTGGCAACCTGACCGCCGAATTTAAAATCTGCCTGACCTTCAGCATGATCAAGAGGCTActg                                                                                                                  >  2:402943/1‑234 (MQ=255)
                                                                                                        aCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACtgg                                                                                                                 >  1:556042/1‑236 (MQ=255)
                                                                                                            cgcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAtt                                                                                                             >  1:350729/1‑236 (MQ=255)
                                                                                                             gcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACCGGAAttg                                                                                                            >  1:262125/1‑236 (MQ=255)
                                                                                                                tcatcaCCCGCATGTTGAAGCTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAt                                                                                                              <  1:1612/231‑1 (MQ=255)
                                                                                                                          cATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATct                                                                                                                                                  >  1:388198/1‑185 (MQ=255)
                                                                                                                          cATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATct                                                                                                                                                  <  2:388198/185‑1 (MQ=255)
                                                                                                                                     tGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAt                                                                                                              >  2:597718/1‑210 (MQ=255)
                                                                                                                                     tGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAAt                                                                                                              <  1:597718/210‑1 (MQ=255)
                                                                                                                                      ggCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGCAAACt                                                                                   >  1:374331/1‑236 (MQ=255)
                                                                                                                                                     taGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGAt                                                                     >  2:59753/1‑235 (MQ=255)
                                                                                                                                                            gCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATc                                                                    >  1:66566/1‑229 (MQ=255)
                                                                                                                                                             ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAAc                                                               >  2:753580/1‑233 (MQ=255)
                                                                                                                                                             ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTgg                                                            >  1:346258/1‑236 (MQ=255)
                                                                                                                                                                                                                       aTTTACCAAGATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGGGCAGCATTCACAGCTGGACCGGGTCGCCAACttta  >  2:548678/1‑235 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                  
GTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTG  >  NC_000913/2020432‑2020882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑