Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,021,050 A→C N322H (AAC→CAC)  fliM → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,021,0500AC98.7% 250.1 / NA 76N322H (AAC→CAC) fliMflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (35/40);  minor base T (1/0);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTG  >  NC_000913/2020818‑2021279
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     
gAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCAcc                                                                                                                                                                                                                                    >  1:158605/1‑236 (MQ=255)
           aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGACCATTTGATTCACCCGATTTTaaa                                                                                                                                                                                                                          >  1:307592/1‑235 (MQ=255)
           aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTTCACCGATTTTCAAt                                                                                                                                                                                                                         >  2:671898/1‑236 (MQ=255)
           aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTaaa                                                                                                                                                                                                                          >  1:302345/1‑235 (MQ=255)
               cctgggccccaaGGTGCAGCATTCCCAGCTGGAGCGGGTCGCCAACTTGGCAGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATtct                                                                                                                                                                                                                      <  2:307592/224‑1 (MQ=255)
               ccTGGTGCCCCAGGTTCAGCATTCCCAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATtct                                                                                                                                                                                                                      <  1:610566/235‑1 (MQ=255)
                cTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGACGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTctct                                                                                                                                                                                                                    <  1:379942/236‑1 (MQ=255)
                         cAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTAAGTATGCGTTACGGTATGACCATTTGTTTCACACGCATTTAAACTCTCTGAACGAgg                                                                                                                                                                                                            >  2:386158/1‑235 (MQ=255)
                                agcatgcaactctggaggtggTCGCCAACTTTGCCAATATCTCGCTACGCCTGTCGCAGATTTTAAACCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGcc                                                                                                                                                                                                     <  2:158605/224‑1 (MQ=255)
                                agcaTTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGccc                                                                                                                                                                                                    >  1:272411/1‑236 (MQ=255)
                                  atttcgcggtaggagctggTCGCCAACTTTGCCTATATCTGCCTCCGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCa                                                                                                                                                                                                   <  2:555867/224‑1 (MQ=255)
                                      cacaGCTGGAGCTGGTCGCCAATTTGGCCGATTCCCCGCTACGCCTGTCGCAGATTTGAAAACTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGa                                                                                                                                                                                              <  2:359212/236‑1 (MQ=255)
                                       acaGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATgcg                                                                                                                                                                                             >  1:223940/1‑234 (MQ=255)
                                        caGTTGGAGCTGGTCGCCAACTTTGCCGATACCTCGCTACGCCTGTCGAAGATTTTAAACCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTTAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGt                                                                                                                                                                                            <  2:64876/236‑1 (MQ=255)
                                            tggagctggTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATTAGTGACa                                                                                                                                                                                        >  1:565054/1‑236 (MQ=255)
                                              gagctggTCACCAACTTAGCCGATACCCCGCTACGCCTGCCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGa                                                                                                                                                                                          <  2:245677/232‑1 (MQ=255)
                                               agctggTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGAc                                                                                                                                                                                         <  1:81346/232‑1 (MQ=255)
                                                gctggTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACTAGGAACAGCCCAAATGAGTGACATGa                                                                                                                                                                                     >  2:607341/1‑235 (MQ=255)
                                                     tCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGaat                                                                                                                                                                                   >  1:245677/1‑232 (MQ=255)
                                                      ccccactttcgCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATc                                                                                                                                                                               <  2:302345/225‑1 (MQ=255)
                                                         cAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCgg                                                                                                                                                                            <  1:380816/235‑1 (MQ=255)
                                                              ttGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATg                                                                                                                                                                      >  2:17761/1‑236 (MQ=255)
                                                                     tttcgcgttACGCCTGTCGGAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGGCGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGAcaacaa                                                                                                                                                               <  1:671898/228‑1 (MQ=255)
                                                                       tctcGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACg                                                                                                                                                             <  1:401654/236‑1 (MQ=255)
                                                                               cGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGGGACATGAATAATCCGGCCGATGCCAACAAAGGCGCACTg                                                                                                                                                     >  2:159081/1‑236 (MQ=255)
                                                                                  cTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGAc                                                                                                                                                  <  1:221702/236‑1 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGcc                                                                                                                                                                                                     >  1:767307/1‑182 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGcc                                                                                                                                                                                                     <  2:767307/182‑1 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACCACAACGGCGCAATGGa                                                                                                                                                   >  1:366236/1‑232 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGa                                                                                                                                                   >  1:306580/1‑232 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGAt                                                                                                                                               >  2:637823/1‑236 (MQ=255)
                                                                                     tCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAAAAGCCCAAATGAGTGACATGAATAATCCTGCCGATGACAACAACGGCGGAATGGACGaa                                                                                                                                               >  2:489837/1‑235 (MQ=255)
                                                                                       gCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCGGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCt                                                                                                                                             <  1:548678/236‑1 (MQ=255)
                                                                                                     tGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTTGCGGCTGAATCGt                                                                                                                               >  2:588647/1‑236 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATc                                                                                                                                                                               <  2:755590/186‑1 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATc                                                                                                                                                                               >  1:755590/1‑186 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCt                                                                                                                                      >  1:692838/1‑227 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTg                                                                                                                             >  2:509467/1‑236 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGAAATGGACGATTGGTGGGCTGAAGCGTTg                                                                                                                             >  1:115580/1‑236 (MQ=255)
                                                                                                       aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAATGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCt                                                                                                                                      <  2:692838/227‑1 (MQ=255)
                                                                                                             ggCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGaa                                                                                                                       <  1:489837/236‑1 (MQ=255)
                                                                                                                 gcGTCCTGCCGATAGAAAAACCCGATCGCATCAGCGCCCATGTTGACGGCGTCCCGGTGCTGACCATTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACaaa                                                                                                                   <  2:375771/235‑1 (MQ=255)
                                                                                                                    tCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATACGGCCGATGACAACAACGGCGCAATGGACGATCTTTGGGCTGAAGCGTTGAGTGAAGaaaaa                                                                                                                 >  2:114943/1‑235 (MQ=255)
                                                                                                                    tCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGAAATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGAACTGTGGGCTGAACCGTTTAGCGAATcaaaa                                                                                                                 >  2:54782/1‑235 (MQ=255)
                                                                                                                              aGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGCCAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACcagcag                                                                                                      >  2:207928/1‑236 (MQ=255)
                                                                                                                                       cGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAgcgc                                                                                             <  1:114943/236‑1 (MQ=255)
                                                                                                                                               tcatcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCgaga                                                                                     <  1:637823/236‑1 (MQ=255)
                                                                                                                                                catcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGAc                                                                                    <  2:306580/236‑1 (MQ=255)
                                                                                                                                                         tGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCa                                                                           >  2:32644/1‑236 (MQ=255)
                                                                                                                                                          gTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCTCCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCCCCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAg                                                                          <  1:470481/236‑1 (MQ=255)
                                                                                                                                                                                      agtATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGaa                                              <  1:54782/236‑1 (MQ=255)
                                                                                                                                                                                        tatgcgaCCCTAAACGGTCAGTATGCGTTACGGATAGAACCTTGGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCgg                                                <  2:565054/226‑1 (MQ=255)
                                                                                                                                                                                        tATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCgg                                                <  1:17761/232‑1 (MQ=255)
                                                                                                                                                                                         aTGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGt                                           >  1:393402/1‑236 (MQ=255)
                                                                                                                                                                                             cACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCa                                       <  1:32644/236‑1 (MQ=255)
                                                                                                                                                                                             cACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGCCGGGGGGGATGTCGACGGACCGtatc                                        >  2:547699/1‑232 (MQ=255)
                                                                                                                                                                                                  tCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAg                                                                                                                           >  2:225215/1‑147 (MQ=255)
                                                                                                                                                                                                  tCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAg                                                                                                                           <  1:225215/147‑1 (MQ=255)
                                                                                                                                                                                                  tCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGGGTTCCAGCAATTTGGCGGTGGGGATGTCAGCGGAAAGTTGCAGGag                                   >  2:51055/1‑234 (MQ=255)
                                                                                                                                                                                                  tCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGAAAAAGGGCTGCGGGGACGGGGTTCCAGCAATTTGCCGGCGGGGATGTTAGCGGACAATTGCAGGata                                  >  2:693796/1‑236 (MQ=255)
                                                                                                                                                                                                       ggTCAGTATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGAc                             <  2:366236/236‑1 (MQ=255)
                                                                                                                                                                                                            atatgacttAAGGACAGAACGTTTGAGTCACCCGATTGTAAATTCTCTGAACGGGGAACAGCCCAACGGAGTGACATGAATAATCCGGCCGATGACAACAAAGGCGCAATGGACGAGCTGTGGGCTGAAGCGTTGAGCGGACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGAcc                            <  2:93443/225‑1 (MQ=255)
                                                                                                                                                                                                             tATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACTAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGAt                        <  1:38620/235‑1 (MQ=255)
                                                                                                                                                                                                             tATGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCAGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCt                           <  1:207928/232‑1 (MQ=255)
                                                                                                                                                                                                              aTGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAAc                                                                                                                      >  2:338595/1‑140 (MQ=255)
                                                                                                                                                                                                              aTGCGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAAc                                                                                                                      <  1:338595/140‑1 (MQ=255)
                                                                                                                                                                                                                 cGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGAt                        <  1:693796/231‑1 (MQ=255)
                                                                                                                                                                                                                 cGTTACGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTAt                     <  2:393402/234‑1 (MQ=255)
                                                                                                                                                                                                                     caggATAGAACATTGGATCCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAAGCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGatat               <  2:380155/234‑1 (MQ=255)
                                                                                                                                                                                                                     aCGGATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGatat               <  1:402943/236‑1 (MQ=255)
                                                                                                                                                                                                                       ggATAGAACATTTGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTTGTGAGTGCAGCGGAACGTTGCAGGGAGTCGCACTGATAATGGac                 >  2:738500/1‑231 (MQ=255)
                                                                                                                                                                                                                             aaCATTTGATTCACCCGATTTTAAATTCTCTGAACGAgg                                                                                                                                                                                                            >  1:42637/1‑39 (MQ=255)
                                                                                                                                                                                                                             aaCATTTGATTCACCCGATTTTAAATTCTCTGAACGAgg                                                                                                                                                                                                            <  2:42637/39‑1 (MQ=255)
                                                                                                                                                                                                                                  ttGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGAt                        >  2:450784/1‑214 (MQ=255)
                                                                                                                                                                                                                                  ttGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGAt                        <  1:450784/214‑1 (MQ=255)
                                                                                                                                                                                                                                  ttGATTCACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGGGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTg  >  2:519224/1‑236 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     
GAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAACAAAAATCAACCAGCAGCAAAAGCGCTGCCGAGACGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTG  >  NC_000913/2020818‑2021279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: