Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,036,444 T→G Y204D (TAT→GAT)  hchA → glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,4440TG100.0% 233.4 / NA 71Y204D (TAT→GAT) hchAglyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/41);  total (30/41)

TGTTCCGCAATCCGAAGAAACTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCA  >  NC_000913/2036211‑2036671
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                   
tgttCCGCAATCCGAAGAAACTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGAt                                                                                                                                                                                                                                   <  1:719559/236‑1 (MQ=255)
       cAATCCGAAGAAACTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGTTAACCCACTGAATGGGGCTTCCAtt                                                                                                                                                                                                                             >  2:38243/1‑235 (MQ=255)
       cAATCCGAAGAAACTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAtt                                                                                                                                                                                                                             >  1:580404/1‑235 (MQ=255)
                    cTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTTTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGTTAACCACCTGAATGGCGATTCCATTTGGGGATTCCCAg                                                                                                                                                                                                                >  2:143215/1‑235 (MQ=255)
                                        ccTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAcgcc                                                                                                                                                                                                           >  1:248670/1‑220 (MQ=255)
                                        ccTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAcgcc                                                                                                                                                                                                           <  2:248670/220‑1 (MQ=255)
                                        ccTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGc                                                                                                                                                                                           >  1:644690/1‑236 (MQ=255)
                                            aaCGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGc                                                                                                                                                                                           <  1:451100/232‑1 (MQ=255)
                                            aaCGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCa                                                                                                                                                                                         <  1:464039/234‑1 (MQ=255)
                                               gCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAt                                                                                                                                                                                    <  2:702187/236‑1 (MQ=255)
                                               gCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAt                                                                                                                                                                                    <  1:713236/236‑1 (MQ=255)
                                                cTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAtt                                                                                                                                                                                   <  1:574322/236‑1 (MQ=255)
                                                   aTAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGCATGGTGATTCCATTTGCGCATTCCCCGACGCCGCAGACAAACAAACGCCAGAGAATGGc                                                                                                                                                                                >  1:285724/1‑236 (MQ=255)
                                                    tAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAtt                                                                                                                                                                                   <  2:285724/232‑1 (MQ=255)
                                                     aGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATCTGGATTTCATTTGCGCATTCCCAGACGGCTCAGACCAACAAACGCCAGACAtttgat                                                                                                                                                                               >  2:419662/1‑231 (MQ=255)
                                                        taaTATGCAGCAGTCTGTGGTCCTGGTGGTCATGGCGCACTTATGGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTatat                                                                                                                                                                           <  2:22106/235‑1 (MQ=255)
                                                         aaTATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                                                                                                                                                         <  2:299500/189‑1 (MQ=255)
                                                         aaTATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                                                                                                                                                         >  1:299500/1‑189 (MQ=255)
                                                         aaTATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATg                                                                                                                                                                          >  1:286140/1‑236 (MQ=255)
                                                             tgccacactcgttGTACCTGGTGCACATGGCCCACTTATTGGTTTGCTTGAAAGCCAGGACGTGGCTGACGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCgg                                                                                                                                                                      <  2:373614/225‑1 (MQ=255)
                                                             tGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCgg                                                                                                                                                                      >  1:22106/1‑236 (MQ=255)
                                                              gcagcaATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCa                                                                                                                                                                                         <  1:664887/216‑1 (MQ=255)
                                                                  caATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTTCCAGACCCCGCAGACAAACAATCCCCAGCGCTTGGCTATATGCCAGGGCAt                                                                                                                                                                 >  1:309161/1‑236 (MQ=255)
                                                                    aTCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCCATATGCCGGGTCAtc                                                                                                                                                                >  1:465905/1‑235 (MQ=255)
                                                                     tCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCCGACGCCGCCGACAACCAAACGCCCGAGAttt                                                                                                                                                                                  >  2:664887/1‑215 (MQ=255)
                                                                      cTTTTTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtc                                                                                                                                                                <  1:143215/233‑1 (MQ=255)
                                                                        ttGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                                                                                                                                                           <  1:443467/236‑1 (MQ=255)
                                                                          gTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                                                                                                                                                           <  1:100773/234‑1 (MQ=255)
                                                                               tggtggTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCCTTCCCCGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGACGGGTCATCTCACCTGGt                                                                                                                                                       >  2:579482/1‑233 (MQ=255)
                                                                                      cATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCga                                                                                                                                             >  2:137388/1‑236 (MQ=255)
                                                                                                   ttGGTTTACCTGAATGCCAGGACGTGGCTGCCGTTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGaaa                                                                                                                                <  1:44824/236‑1 (MQ=255)
                                                                                                   ttGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGaaa                                                                                                                                <  1:38243/236‑1 (MQ=255)
                                                                                                      gTTTCCCTGAATGCCAGTACGGTGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATg                                                                                                                             <  1:277848/236‑1 (MQ=255)
                                                                                                          aCCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCCGAGCTTGGCATAATGCCGGGTCATCTCACCTGGTACTTCGGCGAATTACTGCAGCAAATagggc                                                                                                                         >  2:440641/1‑234 (MQ=255)
                                                                                                          aCCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCa                                                                                                                         >  1:707367/1‑236 (MQ=255)
                                                                                                                      ggACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAGCGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTa                                                                                                             >  2:480628/1‑236 (MQ=255)
                                                                                                                           tGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAatat                                                                                                                  <  2:707367/226‑1 (MQ=255)
                                                                                                                                     tttACAGTGGCCAATCAAATATGACCGTGTTGTTATCTCCCTTGGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCg                                                                                              <  2:558125/236‑1 (MQ=255)
                                                                                                                                        aCAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAGCGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCggg                                                                                            <  1:480628/235‑1 (MQ=255)
                                                                                                                                         cAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCg                                                                                          <  1:745316/236‑1 (MQ=255)
                                                                                                                                              ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGCCAAACAAACGCCAGAGATTGGCTCTATGCCGGGTCATCTCAACTGGTACTTCGGCGAGGAACTGAGGAAATTGGGCATGAATATCATTCATGTCGACATCACCGAGCGAGtcc                                                                                     >  2:446423/1‑234 (MQ=255)
                                                                                                                                              ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAtt                                                                                                                                                                                   <  2:444627/142‑1 (MQ=255)
                                                                                                                                              ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAtt                                                                                                                                                                                   >  1:444627/1‑142 (MQ=255)
                                                                                                                                              ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTAc                                                                                     >  1:270249/1‑236 (MQ=255)
                                                                                                                                                cAATCAAAAATGACCGTTTTGTTAACTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCg                                                                                          <  1:330157/229‑1 (MQ=255)
                                                                                                                                                        atttaCCGTTTTGATAACCGCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAg                                                                                <  2:598194/227‑1 (MQ=255)
                                                                                                                                                              cGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                                                                                                                                                         >  2:720211/1‑88 (MQ=255)
                                                                                                                                                              cGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                                                                                                                                                         <  1:720211/88‑1 (MQ=255)
                                                                                                                                                                           cccTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGAc                                                        <  2:644690/236‑1 (MQ=255)
                                                                                                                                                                             cTTTGCCAGGGCCCGGCGGCTTGTCTGGCGCTTCGGCACGGCGATAACCCTCTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAg                                                      <  2:748475/236‑1 (MQ=255)
                                                                                                                                                                             cTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAg                                                      <  1:421991/236‑1 (MQ=255)
                                                                                                                                                                               ttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAg                                                                                        >  1:39905/1‑200 (MQ=255)
                                                                                                                                                                               ttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAg                                                                                        <  2:39905/200‑1 (MQ=255)
                                                                                                                                                                               ttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGAc                                                        >  1:592987/1‑232 (MQ=255)
                                                                                                                                                                                      ggCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGcc                                                                                                                                                                                          <  2:580753/95‑1 (MQ=255)
                                                                                                                                                                                      ggCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGcc                                                                                                                                                                                          >  1:580753/1‑95 (MQ=255)
                                                                                                                                                                                      ggCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACCTAAGGACCGGAAACTTCTCACCGGCGAAAGTCCTTTTGc                                             >  2:86503/1‑236 (MQ=255)
                                                                                                                                                                                      ggCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGc                                             <  1:440641/236‑1 (MQ=255)
                                                                                                                                                                                      ggCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGc                                             >  1:536673/1‑236 (MQ=255)
                                                                                                                                                                                            gctgcTTGTCTGGCGCTTCGCCACGCCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGa                                        <  2:572777/232‑1 (MQ=255)
                                                                                                                                                                                            gcggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGa                                        <  2:580404/235‑1 (MQ=255)
                                                                                                                                                                                            gcggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGa                                        <  2:270249/235‑1 (MQ=255)
                                                                                                                                                                                                 ttttCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCg                                   <  1:742251/235‑1 (MQ=255)
                                                                                                                                                                                                       ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCAACGGCGACAGGCCCTTTGCAGCGAATGCGTTtggg                             >  2:742251/1‑234 (MQ=255)
                                                                                                                                                                                                             tCGCCACGGCGATAACCCACTGAATGGTGATTCCATGTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTgg                      <  2:286140/236‑1 (MQ=255)
                                                                                                                                                                                                                 cACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                                                                                                                                                         <  1:355535/37‑1 (MQ=255)
                                                                                                                                                                                                                 cACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCAGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTggcggc                  <  1:508156/236‑1 (MQ=255)
                                                                                                                                                                                                                        aTAACCCACTGAATGGTGATTCCATTTGCGCATTccc                                                                                                                                                                                                                  >  2:355535/1‑37 (MQ=255)
                                                                                                                                                                                                                                 tGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCa  <  1:42402/236‑1 (MQ=255)
                                                                                                                                                                                                                                      ggTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCg                                                                                          <  2:405958/143‑1 (MQ=255)
                                                                                                                                                                                                                                      ggTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCg                                                                                          >  1:405958/1‑143 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                   
TGTTCCGCAATCCGAAGAAACTCGCGGATGTTGTTGCCAGCCTCAACGCTGATAGCGAATATGCAGCAATCTTTGTTCCTGGTGGTCATGGCGCACTTATTGGTTTACCTGAAAGCCAGGACGTGGCTGCCGCTTTACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCA  >  NC_000913/2036211‑2036671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: