Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,751 T→G E188D (GAA→GAC gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,7510TG97.5% 261.7 / ‑4.2 81E188D (GAA→GACgmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (38/40);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGC  >  NC_000913/2127520‑2127982
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
cATTTCCACGATCTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCa                                                                                                                                                                                                                                       <  1:765957/235‑1 (MQ=255)
cATTTCCACGAAGTGCCGGAAGGACTACTGACCGCCGTCCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCa                                                                                                                                                                                                                                       <  2:323482/235‑1 (MQ=255)
   ttCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGCGATTTTGCGGGGAACGAAGGATTCGCCACGGAGCGGGGAGTCATGGt                                                                                                                                                                                                                                  >  2:391432/1‑236 (MQ=255)
    tCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGtt                                                                                                                                                                                                                                 <  1:93459/236‑1 (MQ=255)
      cACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGa                                                                                                                                                                                                                               >  1:669346/1‑236 (MQ=255)
       aggAACTGACGCCCGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGaa                                                                                                                                                                                                                              <  1:187464/234‑1 (MQ=255)
       aCGAACTGACGCAAGGAGTACGGACCGCCGGTCGCGATAACGAAAGCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCGTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGaa                                                                                                                                                                                                                              <  2:127153/236‑1 (MQ=255)
         gatcTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAaga                                                                                                                                                                                                                            <  1:765636/233‑1 (MQ=255)
               aCGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTc                                                                                                                                                                                                                      >  1:136561/1‑236 (MQ=255)
               aCGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTc                                                                                                                                                                                                                      >  2:249124/1‑236 (MQ=255)
                    cggaagaCTGAACCTCGTTCGCGTACACGAAATCTTCCGGCTTTTCCTGCTGCAACATCACCCACTGCATTGTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  2:226339/230‑1 (MQ=255)
                    cGGAGTACTGAACGCCGTTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  1:740265/236‑1 (MQ=255)
                    aggAATACTGAACGCCGGTCGCGATAACGAATGCTTCCGGCTGGTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  2:201221/235‑1 (MQ=255)
                      gAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               <  1:235039/236‑1 (MQ=255)
                          aCTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTGCGGGTAACGAAGGGTTCGCCGCGGCGCGGGGAGCATGGGTTTAAGAGAATTCCGtttaatgcg                                                                                                                                                                                                           >  2:137151/1‑229 (MQ=255)
                             gaccGCCGGTCGAGAAACCGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTAc                                                                                                                                                                                                        <  2:669346/233‑1 (MQ=255)
                              aaCGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                                                                                                                                                                       <  1:451119/236‑1 (MQ=255)
                                 gCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGc                                                                                                                                                                                                    >  1:579837/1‑236 (MQ=255)
                                   cGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTTCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCCTGGTTGAAGAGAATTCCGTGAAAGGCGTACATGCCg                                                                                                                                                                                                  >  2:200363/1‑236 (MQ=255)
                                             aaCGAATTCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTCCGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         <  1:371249/235‑1 (MQ=255)
                                             aaCGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                                                                                                                                                                        <  1:249124/236‑1 (MQ=255)
                                                   aTCTTCCGGCTCTTCCGGCTGCAGCTTCAGCCACGGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCAATATTGCCGAGGTACGGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGt                                                                                                                                                                                  <  2:579837/236‑1 (MQ=255)
                                                         cGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGGACAGTCCGTAGGATTTACGGTAGTTAACg                                                                                                                                                                             >  2:40408/1‑235 (MQ=255)
                                                                 ccTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                                                                                                                                                                    <  1:393438/236‑1 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATTTTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGttt                                                                                                                                                      >  2:711023/1‑235 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTg                                                                                                                                                     >  1:618081/1‑236 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACAGGCCGTAGGATTGACGGTAGTTAACGGTGATCCAGTAGCCGTACAGTTTg                                                                                                                                                     >  2:739357/1‑236 (MQ=255)
                                                                                     tGCATTGTTACGTAATCTTTGGCCGGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAc                                                                                                                                                <  2:693680/236‑1 (MQ=255)
                                                                                                 tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGgaga                                                                                                                                    >  1:678233/1‑236 (MQ=255)
                                                                                                      tttGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATcgc                                                                                                                                <  2:678233/235‑1 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                                                                                                <  1:556514/163‑1 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                                                                                                >  2:556514/1‑163 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                                                                                                                                                           >  2:83922/1‑184 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                                                                                                                                                           <  1:83922/184‑1 (MQ=255)
                                                                                                                          gCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACggac                                                                                                                   >  1:363079/1‑226 (MQ=255)
                                                                                                                          gCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTACCGGTAGTTAACGGTGATCCAGTAGGCGTACAGGTTGGCGAACGCATACGGAGATCTCGGGTAGAACGGCGTggagt                                                                                                             >  2:158409/1‑231 (MQ=255)
                                                                                                                              ggaaccctgtttGCCGAGGTCCAGGCCCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCt                                                                                                       <  2:136561/226‑1 (MQ=255)
                                                                                                                                  tCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGTGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCg                                                                                                    <  1:200363/235‑1 (MQ=255)
                                                                                                                                        ttGCCGAGGTACAGGCACGACCCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGCTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGaa                                                                                                 >  1:197814/1‑232 (MQ=255)
                                                                                                                                         tGCCGAGTTAAAGGCACGACCCCAGCCCGTGGGCGATGTTGGCGATGGCGCGGGTGATTTGGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  <  2:575351/230‑1 (MQ=255)
                                                                                                                                           ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTGTCTTTCTGCGGCATTTccg                                                                                           >  2:641651/1‑234 (MQ=255)
                                                                                                                                            cGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGCGATCGCGGGAAGAACGGCGGGGTTCCTTTTTGCGGAATTGCCTGc                                                                                         >  1:340178/1‑236 (MQ=255)
                                                                                                                                             gAGGTACGGGCCCGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:566527/236‑1 (MQ=255)
                                                                                                                                             gAGGTACAGGCACGACTCCAGCCCTTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:641651/236‑1 (MQ=255)
                                                                                                                                             gAGGTACAGGCACGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:137151/236‑1 (MQ=255)
                                                                                                                                              aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        >  1:287093/1‑235 (MQ=255)
                                                                                                                                              aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGGTTGGCGACCGCATACGGCGATCGCGGGTAGAACGGCGTGGTCTCGTTCTGCGGAATTTCCCGCa                                                                                        >  2:24182/1‑235 (MQ=255)
                                                                                                                                                gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt                                                                                           >  1:575351/1‑230 (MQ=255)
                                                                                                                                                   cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:301557/230‑1 (MQ=255)
                                                                                                                                                   cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        >  2:301557/1‑230 (MQ=255)
                                                                                                                                                        aCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc                                                                                                              >  2:489663/1‑203 (MQ=255)
                                                                                                                                                        aCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc                                                                                                              <  1:489663/203‑1 (MQ=255)
                                                                                                                                                          gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGGGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa                                                                              <  2:545234/233‑1 (MQ=255)
                                                                                                                                                          gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAg                                                                           >  1:456483/1‑236 (MQ=255)
                                                                                                                                                           tcTCCAGCCCCTGGGTGATGTTGGCGATGGGGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             <  2:256925/216‑1 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         <  2:649088/125‑1 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         >  1:649088/1‑125 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGt                                                                          >  1:604613/1‑236 (MQ=255)
                                                                                                                                                                  ccccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAcc                                                                                      >  1:256925/1‑217 (MQ=255)
                                                                                                                                                                    ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTg                                                                 >  1:101908/1‑236 (MQ=255)
                                                                                                                                                                      tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGt                                                                  <  2:618081/233‑1 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               >  2:220871/1‑91 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               <  1:220871/91‑1 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGaa                                                              >  1:762707/1‑236 (MQ=255)
                                                                                                                                                                           gaggTTGACGATGGCGCGGGTGATTTGGCGGGTAACGAAGGTTGCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTg                                                                 <  2:545885/226‑1 (MQ=255)
                                                                                                                                                                            aTGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGCTCCAGTAGGCGTACAGTTTGGCGACCGCATACGGGGATCGCGGGTAGAACGGCGTGGCATCTTCCTGCGGCATTTCCTGCACCCAACCATACAGTTCGTAGGTGCAAGCCTg                                                         >  2:72644/1‑236 (MQ=255)
                                                                                                                                                                               ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATa                                                      >  1:430830/1‑236 (MQ=255)
                                                                                                                                                                                tGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACCGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTTCGGAATTTCCTGCGCCAGACCATACAGTTCAGAGGGGGAAGCCTGTTa                                                      >  2:332401/1‑235 (MQ=255)
                                                                                                                                                                                  gcaaTTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGaa                                                   <  1:391432/233‑1 (MQ=255)
                                                                                                                                                                                  gCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGATGTGGAAGCCt                                                          >  1:545885/1‑229 (MQ=255)
                                                                                                                                                                                       tGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAg                                              >  1:247664/1‑236 (MQ=255)
                                                                                                                                                                                           cgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  <  2:636824/180‑1 (MQ=255)
                                                                                                                                                                                           cgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  >  1:636824/1‑180 (MQ=255)
                                                                                                                                                                                                        gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                                                                                                       <  2:207489/130‑1 (MQ=255)
                                                                                                                                                                                                        gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                                                                                                       >  1:207489/1‑130 (MQ=255)
                                                                                                                                                                                                                          cgcggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             <  2:234456/154‑1 (MQ=255)
                                                                                                                                                                                                                          cgcggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             >  1:234456/1‑154 (MQ=255)
                                                                                                                                                                                                                             ggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATc                                                                                                                                  >  2:312295/1‑114 (MQ=255)
                                                                                                                                                                                                                             ggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATc                                                                                                                                  <  1:312295/114‑1 (MQ=255)
                                                                                                                                                                                                                                   gggAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGgcgc  <  2:604613/236‑1 (MQ=255)
                                                                                                                                                                                                                                    gtaTTCATGGTTGACGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGgcgc  <  2:363079/233‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
CATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGC  >  NC_000913/2127520‑2127982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: