Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,185,875 (G)7→8 intergenic (‑79/‑42) rcnR ← / → rcnA transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,8681.G90.2% 327.4 / 28.4 103intergenic (‑72/‑49)rcnR/rcnAtranscriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
Reads supporting (aligned to +/- strand):  ref base . (4/6);  new base G (88/4);  total (92/11)
Fisher's exact test for biased strand distribution p-value = 2.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCA  >  NC_000913/2185769‑2185965
                                                                                                    |                                                                                                     
tCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTGAACAAGAATTTGATAAATCATAGCACTATTAATCTACTGGGGGATAGTATCAGGTACt                                                                                                        <  1:4892414/100‑1 (MQ=255)
 cTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTg                                                                                                      <  1:1509585/100‑1 (MQ=255)
  tGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgg                                                                                                     >  2:4783653/1‑100 (MQ=255)
  tGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgg                                                                                                     <  2:2821435/100‑1 (MQ=255)
   gTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGCAAAATCATAGCACTATTGATCTACTGGGGGGTAGTATCAGGTACTggg                                                                                                   >  1:5304962/1‑100 (MQ=255)
   gTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg                                                                                                   <  2:3737835/100‑1 (MQ=255)
   gTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg                                                                                                   <  1:2436788/100‑1 (MQ=255)
   atttAACACGCATTGAATGAGACATGGCAACACCTTGTTAAAAAAAAAAAGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg                                                                                                   <  2:908339/99‑1 (MQ=255)
    tttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATGTGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggg                                                                                                 >  2:1557044/1‑100 (MQ=255)
    tttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAAAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggg                                                                                                 <  2:3538352/100‑1 (MQ=255)
     ttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggg                                                                                                >  1:1319076/1‑100 (MQ=255)
     ttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggg                                                                                                <  2:314367/100‑1 (MQ=255)
      tATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggggg                                                                                              >  2:415146/1‑100 (MQ=255)
      tATCACGGATTGTATGAGACATAGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggggg                                                                                              <  2:5015847/100‑1 (MQ=255)
      tATCACGGATTGTATGAGAAATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggggg                                                                                              <  1:4223620/100‑1 (MQ=255)
       aTCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggg                                                                                             >  2:5378584/1‑100 (MQ=255)
         cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggggggga                                                                                           >  2:5051191/1‑98 (MQ=255)
          aCGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggggag                                                                                          >  1:1522292/1‑97 (MQ=255)
           cGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggggagt                                                                                         >  1:5571285/1‑96 (MQ=255)
            ggATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTTCTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAg                                                                                       <  2:5290162/100‑1 (MQ=255)
            ggATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggggagta                                                                                        >  1:5015434/1‑95 (MQ=255)
             gATTGTATGGTACGTGACAACACCTTGTCAACAAGAAGATGAAAAATCATAGCACTATTAAGCTACTGGGGGGAAGTATCTGGTACTGGGGGGGGAGTAg                                                                                       <  2:3985126/100‑1 (MQ=255)
               ttGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGaa                                                                                     >  2:5480362/1‑100 (MQ=255)
                 gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATc                                                                                   >  2:3191704/1‑100 (MQ=255)
                 gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATc                                                                                   >  2:2243934/1‑100 (MQ=255)
                  tATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCa                                                                                  >  1:2665650/1‑100 (MQ=255)
                   aTGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAg                                                                                 >  1:962200/1‑100 (MQ=255)
                    tGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGCGTAGAATCAGa                                                                                >  1:2765929/1‑100 (MQ=255)
                    tGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGa                                                                                >  1:3806055/1‑100 (MQ=255)
                    tGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGa                                                                                >  1:2837413/1‑100 (MQ=255)
                     gagaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGAt                                                                               >  2:1985469/1‑100 (MQ=255)
                     gagaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGAt                                                                               >  2:4594636/1‑100 (MQ=255)
                     gagaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGAt                                                                               >  1:239261/1‑100 (MQ=255)
                      agaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGAtt                                                                              >  1:5168030/1‑100 (MQ=255)
                         cATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGcc                                                                           >  1:4009409/1‑100 (MQ=255)
                         cATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGcc                                                                           >  1:1128172/1‑100 (MQ=255)
                         cATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGcc                                                                           >  2:1401935/1‑100 (MQ=255)
                          aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCg                                                                          >  2:1387481/1‑100 (MQ=255)
                          aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGCAGAATCAGATTGCCg                                                                          >  1:5564019/1‑100 (MQ=255)
                           tGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGa                                                                         >  1:3714265/1‑100 (MQ=255)
                            agCAAAACCTGGTTAACAAGAGTATGAAAATTCATGCCACTATTAAGCTTCTGGGGGGTAGTATGTGGTAGTGGGGGGGAGTAGAATCAGATTGCCGAAt                                                                       <  1:3126276/99‑1 (MQ=255)
                             gCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAAt                                                                       >  1:1979973/1‑100 (MQ=255)
                             gCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAAt                                                                       >  1:3904357/1‑100 (MQ=255)
                                 cacCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAAt                                                                   >  1:2208095/1‑100 (MQ=255)
                                 cacCTGGTTAACAAGAAGATGAAAAATCATATCATTATTATTCTACTGGGGGATAGTATCAGGGACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAAt                                                                   <  2:4348656/100‑1 (MQ=255)
                                   ccTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATAc                                                                 >  2:1596671/1‑100 (MQ=255)
                                    cTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                >  2:4245086/1‑100 (MQ=255)
                                    cTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                >  1:2373237/1‑100 (MQ=255)
                                       gTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAg                                                             >  2:3946984/1‑100 (MQ=255)
                                           aCAAGAATATGAAACATCATAGAACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGAGGAAGAAGAATCAGATTGCCGAATTCCTGCTAAAAat                                                          >  2:530553/1‑100 (MQ=255)
                                            cAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                        >  1:5286936/1‑100 (MQ=255)
                                             aaGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattat                                                       >  1:3010819/1‑100 (MQ=255)
                                              aGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                                      >  2:1830506/1‑100 (MQ=255)
                                              aGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                                      >  1:994531/1‑100 (MQ=255)
                                              aGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                                      >  2:2902402/1‑100 (MQ=255)
                                              aGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                                      >  2:4292794/1‑100 (MQ=255)
                                                 agatGAAAAATCATAGTACTAGTAGCGGAAGTGGGGTTAGTATCAGGTACTAGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATc                                                   <  2:1545403/98‑1 (MQ=255)
                                                    tGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATg                                                <  1:843480/100‑1 (MQ=255)
                                                     gAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGa                                               >  1:1817286/1‑100 (MQ=255)
                                                      aaaaaTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                              >  2:3897017/1‑100 (MQ=255)
                                                             aTAGCACTATTAATCTACTGGGGGGTATTATCAGGTACTGGGGGGGGAGTAGAATCAGAGTGCCGAATTAATACTAAGAATTATTATCATGACCGAAttt                                       >  1:4914565/1‑100 (MQ=255)
                                                             aTAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAAttt                                       >  1:1964471/1‑100 (MQ=255)
                                                             aTAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAAttt                                       >  2:1980612/1‑100 (MQ=255)
                                                              tAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTa                                      >  2:670871/1‑100 (MQ=255)
                                                                gCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACa                                    >  1:3291937/1‑100 (MQ=255)
                                                                 cACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACaa                                   >  2:862681/1‑100 (MQ=255)
                                                                  aCTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                  >  2:1826497/1‑100 (MQ=255)
                                                                   cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAct                                 >  1:4041883/1‑100 (MQ=255)
                                                                   cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAct                                 >  2:49603/1‑100 (MQ=255)
                                                                    tATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                >  1:1084946/1‑100 (MQ=255)
                                                                     aTTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActct                               >  2:2170135/1‑100 (MQ=255)
                                                                         aTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                           >  2:5397912/1‑100 (MQ=255)
                                                                         aTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                           >  2:3989228/1‑100 (MQ=255)
                                                                         aTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                           >  1:1640877/1‑100 (MQ=255)
                                                                            tACTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAg                        >  2:3668071/1‑100 (MQ=255)
                                                                              cTGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCttcttcttgt                      >  1:1049311/1‑96 (MQ=255)
                                                                               tGGGGGGTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCaa                     >  1:675936/1‑100 (MQ=255)
                                                                                 gggggTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAgg                   >  1:4553254/1‑100 (MQ=255)
                                                                                  ggggTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGa                  >  2:3164629/1‑100 (MQ=255)
                                                                                  cggttagttTCAAGTACAGGGTGGTAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGa                  <  2:1243275/96‑1 (MQ=255)
                                                                                   gggTAGTATCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGaa                 >  2:1856844/1‑100 (MQ=255)
                                                                                   gggTAGTATCAGGTACTGGTGGGGGCGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCATCTTCAGCAAGGaa                 >  2:5024930/1‑100 (MQ=255)
                                                                                     gtagtaTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAAc               >  2:4012551/1‑100 (MQ=255)
                                                                                       agtaTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGc             >  2:2060869/1‑100 (MQ=255)
                                                                                        gtaTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGcc            >  2:1181638/1‑100 (MQ=255)
                                                                                        gtaTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGcc            >  1:1949084/1‑100 (MQ=255)
                                                                                         taTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCt           >  2:783683/1‑100 (MQ=255)
                                                                                          aTCATGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg          >  1:2718333/1‑100 (MQ=255)
                                                                                          aTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg          >  2:3501029/1‑100 (MQ=255)
                                                                                          aTCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg          >  2:3380239/1‑100 (MQ=255)
                                                                                           tCAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTgg         >  2:2412626/1‑100 (MQ=255)
                                                                                            cAGGTACTGGGGGGGGAGTAGAATCGGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt        >  2:74173/1‑100 (MQ=255)
                                                                                            cAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt        >  1:1222756/1‑100 (MQ=255)
                                                                                            cAGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt        >  2:2923108/1‑100 (MQ=255)
                                                                                             aGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGtt       >  2:2543883/1‑100 (MQ=255)
                                                                                             aGGTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGtt       >  2:4094335/1‑100 (MQ=255)
                                                                                              ggTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttc      >  1:321985/1‑100 (MQ=255)
                                                                                               gTACTGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttct     >  2:1638881/1‑100 (MQ=255)
                                                                                                 tgtGGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCa  <  1:2007141/98‑1 (MQ=255)
                                                                                                 gttGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCa  <  1:3511764/98‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCA  >  NC_000913/2185769‑2185965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: