Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,288,561 (C)7→8 pseudogene (354/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,288,5541.C93.8% 526.3 / 28.1 160pseudogene (361/2525 nt)yejOpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base . (7/3);  new base C (80/70);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

GTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGT  >  NC_000913/2288455‑2288648
                                                                                                       |                                                                                                 
gttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACgg                                                                                                    <  2:4872847/100‑1 (MQ=255)
gttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACgg                                                                                                    <  1:148648/100‑1 (MQ=255)
     tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTATTCCTTCCTTTTCGATAGCGACAAAGACGG‑‑ccccc                                                                                           <  2:2251759/100‑1 (MQ=255)
     tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccc                                                                                           <  1:2624273/100‑1 (MQ=255)
     tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccc                                                                                           >  1:775580/1‑100 (MQ=255)
     tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAAACTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccc                                                                                           >  2:867832/1‑100 (MQ=255)
       tAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCGCCCTCTTTAACCTCCAGTAGTCCTCCGTTTTCGATAGCGACAAAGATGT‑‑ccccccc                                                                                         <  2:4532930/100‑1 (MQ=255)
       tAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccccn                                                                                         <  1:4440920/100‑2 (MQ=255)
       tAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccccc                                                                                         <  1:2972760/100‑1 (MQ=255)
        aaTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑cccccccc                                                                                        <  2:2301597/100‑2 (MQ=255)
        aaTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACATCCAGCAGTCCTCCCTTTACGATAGCGACAAAGACGG‑‑cccccccc                                                                                        >  1:5211187/1‑99 (MQ=255)
         aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTTTTTATCTTCAAAAACGG‑‑cccccccca                                                                                       >  2:791652/1‑98 (MQ=255)
           aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑ccccccccatc                                                                                     >  1:4262509/1‑96 (MQ=255)
            gCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTCGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑CCCCCCCATCtt                                                                                    >  2:1816110/1‑100 (MQ=255)
                cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCttttt                                                                                 >  1:4074504/1‑100 (MQ=255)
                 gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATCGCGACAAAGTCTGC‑‑CCCCCCCATCttcttc                                                                                >  2:1729296/1‑100 (MQ=255)
                 gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTc                                                                                <  1:638745/100‑1 (MQ=255)
                  ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCa                                                                               <  1:30694/100‑1 (MQ=255)
                   cTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCaa                                                                              >  1:5376551/1‑100 (MQ=255)
                    tGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAAc                                                                             <  2:4952889/100‑1 (MQ=255)
                    tGCTTTCCGATCTACCGCTATTGCAATTCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGTCAAATACGGCCCCCCCCATCTTTTTCAAc                                                                             <  1:5206797/100‑1 (MQ=255)
                    ngCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAAc                                                                             >  1:4447781/2‑100 (MQ=255)
                     gCTTTCTGTCCTCCCGCAAAGGAAATCCCCCCTCCTTTACCCTCCAGTATTCCCCCCTTTTCGATAGGGAAAAAGAGGGCCCCCCCCATCTTTTTCAACg                                                                            <  2:3619509/100‑1 (MQ=255)
                     gCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCACCg                                                                            >  1:968318/1‑100 (MQ=255)
                     gCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACg                                                                            >  2:2162118/1‑100 (MQ=255)
                       tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGAt                                                                          >  2:4574133/1‑100 (MQ=255)
                        ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGAtt                                                                         <  2:4416830/100‑1 (MQ=255)
                           tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAg                                                                      >  1:4853252/1‑100 (MQ=255)
                            gATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAgc                                                                     >  1:2247780/1‑100 (MQ=255)
                             aTCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAgcg                                                                    >  2:5021004/1‑100 (MQ=255)
                              tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAACGACGGCCCCCCCCATCTTTTTCAACGATTAAgcgc                                                                   >  2:1087805/1‑100 (MQ=255)
                               cTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAAcgcc                                                                  <  1:3880558/100‑1 (MQ=255)
                                 aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGcgcccg                                                                >  1:4126300/1‑100 (MQ=255)
                                  ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGcgcccgc                                                               >  2:4267306/1‑100 (MQ=255)
                                  ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACCATTACGcgcccgc                                                               >  2:4744901/1‑100 (MQ=255)
                                     caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                            >  2:2699439/1‑100 (MQ=255)
                                       aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTa                                                          >  2:3154424/1‑100 (MQ=255)
                                       aatgcaaatCCCCCCTCTTTAACCGCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCttt                                                           <  2:4852931/100‑1 (MQ=255)
                                          ncaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCGGCCGTTAATc                                                       >  1:4452904/2‑100 (MQ=255)
                                           caaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                      >  1:3598932/1‑100 (MQ=255)
                                             aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                    <  1:3294911/100‑1 (MQ=255)
                                                ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTa                                                 >  1:1140089/1‑100 (MQ=255)
                                                  ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATTTTTTTCAACGATTACGCGCCCGCCTTTAATCGTGGTAtg                                               >  1:3967757/1‑99 (MQ=255)
                                                  ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                               <  1:4654034/100‑1 (MQ=255)
                                                  ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                               >  1:4008810/1‑100 (MQ=255)
                                                   cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCa                                              >  1:3122597/1‑100 (MQ=255)
                                                      tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCTACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                           <  2:4634412/100‑1 (MQ=255)
                                                      tctTAACCCTCCAGTAGACCTCCCTTTTCGATAGCGAAAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                           <  2:2791369/100‑1 (MQ=255)
                                                        ctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                          >  1:4533395/1‑100 (MQ=255)
                                                         tttAAACTCCAGTAGTCCTCCTTTTTCAATAACGACAAAGTCGT‑‑CTCGCCCCATTTTTTTCAACGATTAAACGCCCGCCTTTAATCGTCGTATCATTCGc                                         >  2:327843/1‑100 (MQ=255)
                                                           tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                       >  1:1891603/1‑100 (MQ=255)
                                                            aaCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGAC‑‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                      <  2:4760059/100‑1 (MQ=255)
                                                            aaCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                      >  1:4776763/1‑100 (MQ=255)
                                                             aCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGGATCATTCGCTgcg                                     >  1:2136679/1‑100 (MQ=255)
                                                             aCCTCCAGTAGTCCCCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcg                                     <  2:3126199/100‑1 (MQ=255)
                                                              ccTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTACTCGTGGTATCATTCGCTgcgc                                    >  2:2108890/1‑100 (MQ=255)
                                                              ccTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcgc                                    >  1:1670656/1‑100 (MQ=255)
                                                              ccTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcgc                                    >  2:3693913/1‑100 (MQ=255)
                                                              ccTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGcc                                   <  1:1836764/100‑1 (MQ=255)
                                                               cTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGcc                                   <  1:2500096/100‑1 (MQ=255)
                                                               cTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATAATTCGCTGCGcc                                   <  2:3662837/100‑1 (MQ=255)
                                                                tCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                  <  1:1014818/100‑1 (MQ=255)
                                                                tCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑CCACACCCATCTTTTTCAACGATTAAGCGCCGGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                  <  2:4410500/100‑1 (MQ=255)
                                                                   agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                               <  2:1706479/100‑1 (MQ=255)
                                                                    gtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaaa                              <  2:3447882/100‑1 (MQ=255)
                                                                    gtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaaa                              <  2:2319491/100‑1 (MQ=255)
                                                                    gtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCAACTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaaa                              <  2:2985558/100‑1 (MQ=255)
                                                                      atctctccCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc                            <  1:3475252/96‑1 (MQ=255)
                                                                      agtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc                            <  1:1209988/100‑1 (MQ=255)
                                                                      agtCCTCCCTTTTCAATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAtttacc                            <  2:3482586/100‑6 (MQ=255)
                                                                         cctccCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGt                         <  1:2648791/100‑1 (MQ=255)
                                                                          ctccCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAccagat                        >  2:3442977/1‑96 (MQ=255)
                                                                            cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCGTTCGCTGCGCCATAAACCTGTAGc                      <  1:388531/100‑1 (MQ=255)
                                                                            cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGc                      >  1:1314074/1‑100 (MQ=255)
                                                                            cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGc                      <  1:1451637/100‑1 (MQ=255)
                                                                            cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGc                      >  1:4991283/1‑100 (MQ=255)
                                                                              cTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcac                    >  2:3398383/1‑100 (MQ=255)
                                                                              cTTTTCGATAGCGACAAAGACGG‑‑CCGCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTCTAGcac                    >  1:2902904/1‑100 (MQ=255)
                                                                                gtacGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                  <  2:3931599/97‑1 (MQ=255)
                                                                                 ttCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc                 <  1:1104169/100‑1 (MQ=255)
                                                                                  tCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                <  2:1309069/100‑1 (MQ=255)
                                                                                    gATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatt              >  2:2091433/1‑100 (MQ=255)
                                                                                    gATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatt              >  1:511450/1‑100 (MQ=255)
                                                                                     aTAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatta             <  1:3897894/100‑1 (MQ=255)
                                                                                     aTAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatta             >  1:5504952/1‑100 (MQ=255)
                                                                                        gCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTg          >  2:2698171/1‑100 (MQ=255)
                                                                                         cGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa         >  1:945374/1‑100 (MQ=255)
                                                                                          gACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTCGCACACCATTATTGAg        <  2:5312900/100‑1 (MQ=255)
                                                                                          gACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg        <  2:4710880/100‑1 (MQ=255)
                                                                                          gACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg        <  2:3845283/100‑1 (MQ=255)
                                                                                          gACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCATTAATCGTGGTATCATTTGCTGCGCCATAAACCTGTAGCACACCATTATTGAg        >  1:1603769/1‑100 (MQ=255)
                                                                                          gACAAAGACGGCCCCCCCCATCCTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg        <  2:248438/100‑1 (MQ=255)
                                                                                           aCAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGTTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGc       <  1:4887256/100‑1 (MQ=255)
                                                                                           aCAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGc       <  1:1315472/100‑1 (MQ=255)
                                                                                           aCAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGc       >  2:1503221/1‑100 (MQ=255)
                                                                                            cAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTTGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg      <  2:5100213/100‑1 (MQ=255)
                                                                                            cAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg      >  2:2573201/1‑100 (MQ=255)
                                                                                             aaaGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt     >  2:5335394/1‑100 (MQ=255)
                                                                                              aaGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTTGCACACCATTTTTGAGCGTa    >  1:201757/1‑100 (MQ=255)
                                                                                              aaGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa    <  2:5415722/100‑1 (MQ=255)
                                                                                                       |                                                                                                 
GTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGT  >  NC_000913/2288455‑2288648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: