Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,769,648 (C)6→7 intergenic (+162/‑55) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,6421.C95.6% 612.3 / 21.1 183intergenic (+156/‑61)yfjQ/yfjRCP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (5/3);  new base C (89/86);  total (94/89)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 212 total aligned reads displayed.

CTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGG  >  NC_000913/2769544‑2769736
                                                                                                   |                                                                                                  
cTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGc                                                                                                   <  1:5401794/100‑1 (MQ=255)
    tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc                                                                                              <  2:778635/100‑1 (MQ=255)
     gtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑c                                                                                          <  1:555061/100‑1 (MQ=255)
     gtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCATGTCCGccccc‑‑‑c                                                                                          >  1:136532/1‑100 (MQ=255)
      tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cc                                                                                         <  1:4699746/100‑2 (MQ=255)
      tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAAGAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cc                                                                                         >  1:1551618/1‑99 (MQ=255)
       gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cct                                                                                        >  2:1638213/1‑98 (MQ=255)
       gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cct                                                                                        >  1:2015230/1‑98 (MQ=255)
        ccTCAATCCCCCGGTTATAGCCTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTCCGTTGTCCATATCCTGTCCGccccc‑‑‑ccta                                                                                       >  1:684998/1‑97 (MQ=25)
         cTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cctct                                                                                      <  2:1807733/100‑5 (MQ=255)
          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cctctt                                                                                     <  1:3590431/100‑6 (MQ=255)
          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccc‑‑‑cctctt                                                                                     <  2:782290/100‑6 (MQ=255)
          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCC‑‑‑CTCttt                                                                                     <  2:4402322/100‑1 (MQ=255)
           cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCttt                                                                                     <  1:4277976/100‑1 (MQ=255)
           cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCttt                                                                                     <  2:1024902/100‑1 (MQ=255)
            aaTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTa                                                                                    <  2:273439/100‑1 (MQ=255)
            aaTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTa                                                                                    <  2:3258421/100‑1 (MQ=255)
             aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTaa                                                                                   <  2:354356/100‑1 (MQ=255)
             aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTaa                                                                                   <  1:1338669/100‑1 (MQ=255)
              tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTaaa                                                                                  <  1:1500436/100‑1 (MQ=255)
               cccccGGTTTTAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAg                                                                                 <  2:4766408/100‑1 (MQ=255)
                ccccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGt                                                                                <  2:1850819/100‑1 (MQ=255)
                ccccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGt                                                                                <  2:3639695/100‑1 (MQ=255)
                 cccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTa                                                                               >  1:2597833/1‑100 (MQ=255)
                  ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTaa                                                                              <  2:5094422/100‑1 (MQ=255)
                   cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAAt                                                                             <  2:2153741/100‑1 (MQ=255)
                    ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATc                                                                            <  1:2779692/100‑1 (MQ=255)
                    ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATc                                                                            <  1:1861383/100‑1 (MQ=255)
                     gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATca                                                                           >  2:4145499/1‑100 (MQ=255)
                         taGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCAcatc                                                                       >  2:5430801/1‑100 (MQ=255)
                          aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCAcatca                                                                      >  2:3974652/1‑100 (MQ=255)
                          aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCAcatca                                                                      >  1:970003/1‑100 (MQ=255)
                             ttttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCAttt                                                                   >  2:2385589/1‑100 (MQ=255)
                             ttttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCAttt                                                                   >  2:3883776/1‑100 (MQ=255)
                              tttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCAtttt                                                                  >  1:5026320/1‑100 (MQ=255)
                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:3381786/100‑1 (MQ=255)
                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:1315528/100‑1 (MQ=255)
                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:4526389/100‑1 (MQ=255)
                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTCTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  1:4980503/100‑1 (MQ=255)
                                 aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcag                                                               <  1:462775/100‑1 (MQ=255)
                                   cccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtc                                                             >  1:3200778/1‑100 (MQ=255)
                                   cccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtc                                                             >  1:4131306/1‑100 (MQ=255)
                                     cccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtcag                                                           >  1:3469479/1‑100 (MQ=255)
                                        gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                        >  2:3527953/1‑100 (MQ=255)
                                        gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                        <  2:718756/100‑1 (MQ=255)
                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  1:3818624/100‑1 (MQ=255)
                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  1:754451/100‑1 (MQ=255)
                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  2:3779433/100‑1 (MQ=255)
                                            naTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCGGCCCCCC‑‑‑CTCTGTAAAGTAATGACATCATATTCAGTCAGTTAACtt                                                    >  1:4455262/2‑100 (MQ=14)
                                            cATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                    >  1:4061265/1‑100 (MQ=255)
                                            cATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                    <  1:2333960/100‑1 (MQ=255)
                                              tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTc                                                  >  2:4625932/1‑100 (MQ=255)
                                              tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTc                                                  >  1:5230952/1‑100 (MQ=255)
                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 <  2:3707292/100‑1 (MQ=255)
                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 >  1:1593945/1‑100 (MQ=255)
                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 <  1:5004787/100‑1 (MQ=255)
                                                   cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGga                                             >  2:844475/1‑100 (MQ=255)
                                                    ttttGCAGAAATAAAACATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  2:939989/100‑1 (MQ=17)
                                                    ttttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  1:2426256/100‑1 (MQ=255)
                                                    ttttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  2:4024921/100‑1 (MQ=255)
                                                      ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                          <  1:3917363/100‑1 (MQ=255)
                                                       tGCACAAATAAAAAATAGTTTCTTCCTTCTACATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAATTAATCACATCATTTTCAGTCAGTTAACTTTCCTTGAGAAt                                         <  1:4826817/100‑1 (MQ=17)
                                                        gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtc                                        >  1:402955/1‑100 (MQ=255)
                                                         cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtct                                       <  2:4900141/100‑1 (MQ=255)
                                                          aGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGCAtctc                                      >  1:2234509/1‑100 (MQ=255)
                                                            aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                    <  1:1657954/100‑1 (MQ=255)
                                                            aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                    <  2:3318100/100‑1 (MQ=255)
                                                             aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCa                                   <  1:2155940/100‑1 (MQ=255)
                                                             aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCa                                   >  2:1960419/1‑100 (MQ=255)
                                                              aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTGCGCCCCCC‑‑‑CTCTTTAAAGAAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                  >  1:5171059/1‑100 (MQ=21)
                                                              aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCACCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                  >  2:4226150/1‑100 (MQ=255)
                                                                 aaaaaTAGTTTCTGCGTTATCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                               >  1:70271/1‑100 (MQ=255)
                                                                  aaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCGTTTGCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGaca                              <  2:5159506/100‑1 (MQ=18)
                                                                    aaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAcaca                            <  2:4093962/100‑1 (MQ=255)
                                                                       aGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGc                         <  2:1442675/100‑1 (MQ=255)
                                                                        ntttCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        >  1:271769/2‑100 (MQ=255)
                                                                        gTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        >  1:2960399/1‑100 (MQ=255)
                                                                        gTTTCTGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        <  1:4520581/100‑1 (MQ=255)
                                                                            ccacGTTGTCCATACCCGGTCCGCCCCCC‑‑‑CTCTTAAAATTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAc                    <  2:2523403/97‑1 (MQ=17)
                                                                             tGCGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcg                   <  1:3382082/100‑1 (MQ=255)
                                                                             tGCGTTGTCCATACCCTGTCCGCCCCCCCTCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGaa                     >  2:4854395/1‑100 (MQ=18)
                                                                               cGTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc                 >  2:3155110/1‑100 (MQ=255)
                                                                                gTTGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                >  2:851524/1‑100 (MQ=255)
                                                                                  tGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACCCAGGCAGAACgccgcc              >  2:2747963/1‑100 (MQ=255)
                                                                                  tGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              >  1:3705234/1‑100 (MQ=255)
                                                                                  tGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              <  1:2097062/100‑1 (MQ=255)
                                                                                  tGTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              <  2:3071686/100‑1 (MQ=255)
                                                                                   gTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCa             >  2:2988808/1‑100 (MQ=255)
                                                                                   gTCCATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCa             >  2:555973/1‑100 (MQ=255)
                                                                                    tCCATACCCTGTCCGCCCCCA‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCAt            >  1:4346874/1‑100 (MQ=255)
                                                                                     ccttgcccTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTGAGTTTACTTTCCTGGAGAAGCTCTCATGACACAGGCAGAACGCGGCCATg           >  2:5176639/6‑100 (MQ=17)
                                                                                     ccATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATg           >  2:2845233/1‑100 (MQ=255)
                                                                                      cATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          <  1:2310023/100‑1 (MQ=255)
                                                                                      cATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          >  1:1031872/1‑100 (MQ=255)
                                                                                      cATACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          >  2:4062060/1‑100 (MQ=255)
                                                                                       aTACCCTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAc         >  2:5033787/1‑100 (MQ=255)
                                                                                          cccTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg      >  2:2125575/1‑100 (MQ=255)
                                                                                            cTGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggct    <  1:3068128/100‑1 (MQ=255)
                                                                                             tGTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctg   <  2:2595116/100‑1 (MQ=255)
                                                                                              gTCCGCCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctgg  >  1:3310036/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGG  >  NC_000913/2769544‑2769736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: