Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,999,680 (G)7→8 coding (212/756 nt) ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,999,6731.G93.1% 577.9 / 35.9 176coding (219/756 nt)ygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (4/8);  new base G (73/90);  total (78/98)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGT  >  NC_000913/2999574‑2999767
                                                                                                    |                                                                                                 
cGATGCGTTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTaa                                                                                                    <  2:1116118/100‑1 (MQ=255)
 gATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGCCCAACTTCAATGGTGTAA‑‑g                                                                                                 >  1:3979357/1‑100 (MQ=255)
 gATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑g                                                                                                 >  2:2464090/1‑100 (MQ=255)
  aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                <  1:1099399/100‑1 (MQ=255)
  aTGCGGTTTTGGTCGTTGATTTGACGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                <  2:2156691/100‑1 (MQ=255)
   tGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                              <  2:3937558/100‑1 (MQ=255)
   tGCGGTTTTGGTCGATGATTTGGCGGCTGATTTACGTGTAATACTGCCACTTTTAGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                              >  2:5141461/1‑100 (MQ=255)
     cGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggggg                                                                                           >  2:1788729/1‑100 (MQ=255)
       gTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggggggg                                                                                         <  2:1852082/100‑1 (MQ=255)
        ttttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gggggggg                                                                                        <  1:1880567/100‑2 (MQ=255)
        ttttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gggggggg                                                                                        >  1:4637377/1‑99 (MQ=255)
         tttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gggggggga                                                                                       <  1:3797199/100‑3 (MQ=255)
           tGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGgaaa                                                                                     <  1:5043133/100‑2 (MQ=255)
            ggTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGgaaat                                                                                    <  2:3348220/100‑3 (MQ=255)
            ggTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGgaaat                                                                                    <  1:1362576/100‑3 (MQ=255)
             gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATg                                                                                    >  2:1723733/1‑100 (MQ=255)
             gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTGCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATg                                                                                    <  1:5358870/100‑1 (MQ=255)
              tCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGc                                                                                   <  2:1476579/100‑1 (MQ=255)
                gTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCg                                                                                 <  2:2666283/100‑1 (MQ=255)
                gTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCg                                                                                 >  1:40205/1‑100 (MQ=255)
                gTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCg                                                                                 >  1:3584977/1‑100 (MQ=255)
                gTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCg                                                                                 >  2:720562/1‑100 (MQ=255)
                 ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGt                                                                                <  1:3661412/100‑1 (MQ=255)
                 ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGt                                                                                <  1:3009509/100‑1 (MQ=255)
                 ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGt                                                                                <  2:1761745/100‑1 (MQ=255)
                  tGATTTGGCGGTTTATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGtt                                                                               <  2:3856185/100‑1 (MQ=255)
                   gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTc                                                                              <  1:5503338/100‑1 (MQ=255)
                   gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTc                                                                              <  1:2482115/100‑1 (MQ=255)
                      ttGGCGGTTGATTTACGTGTGATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGCAATGCCGTTCAGt                                                                           >  1:4339927/1‑100 (MQ=255)
                      ttGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGt                                                                           <  2:521198/100‑1 (MQ=255)
                       tGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTc                                                                          >  1:1957577/1‑100 (MQ=255)
                        ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTcg                                                                         >  2:2270936/1‑100 (MQ=255)
                          cGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTcgcg                                                                       <  2:1048986/100‑1 (MQ=255)
                          cGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTcgcg                                                                       <  2:4232950/100‑1 (MQ=255)
                           ggTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGGCGTTCAGTCgcgc                                                                      >  2:4316232/1‑100 (MQ=255)
                            gTTGATTTACGTGTAATACTGCTACTTTTCGCCCCCCCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGcc                                                                     <  1:3155561/100‑1 (MQ=255)
                               gATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGt                                                                  >  2:1443387/1‑100 (MQ=255)
                                aTTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGtt                                                                 >  1:1354069/1‑100 (MQ=255)
                                aTTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTGCAATGGTGTAATGGGGGGGAAATGCCGGTCAGTCGCGCCAggt                                                                 >  2:249890/1‑98 (MQ=255)
                                  ttACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCt                                                               >  1:2367404/1‑100 (MQ=255)
                                     cGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCtttt                                                            <  1:4511954/100‑1 (MQ=255)
                                      gtgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                           <  2:1039131/100‑1 (MQ=255)
                                      gtgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                           >  2:845705/1‑100 (MQ=255)
                                      gtgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                           >  2:5020078/1‑100 (MQ=255)
                                      ggttAATACGTCTACTCTTCGCCCCGCTCATTTTTTGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                           <  2:2753858/97‑1 (MQ=255)
                                       tgtAATACTGCTGCTGTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTGCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTAc                                                          <  2:231780/100‑1 (MQ=255)
                                       tgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                           >  1:4166623/1‑100 (MQ=255)
                                        gtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGCAATGCCGTTCAGTCGCGCCAGTTCTTTTACg                                                         >  1:1849213/1‑100 (MQ=255)
                                         tAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGc                                                        <  1:5329199/100‑1 (MQ=255)
                                           aTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTg                                                      <  1:4556630/100‑1 (MQ=255)
                                           aTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTg                                                      <  1:5291042/100‑1 (MQ=255)
                                            tACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCTGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                     >  1:4732846/1‑100 (MQ=255)
                                            tACTGCTACTTTTCGCCCCACCCAGTTTTAGCTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                     <  1:259980/100‑1 (MQ=255)
                                              cTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGtt                                                   >  2:2598903/1‑100 (MQ=255)
                                                gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTcc                                                 >  2:3173887/1‑100 (MQ=255)
                                                 cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTccc                                                >  2:1554640/1‑100 (MQ=255)
                                                   aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgt                                              >  1:5168219/1‑100 (MQ=255)
                                                    cTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtg                                             <  2:1936175/100‑1 (MQ=255)
                                                    cTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtg                                             >  1:3259666/1‑100 (MQ=255)
                                                     ttttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtgg                                            >  1:2542407/1‑100 (MQ=255)
                                                      tttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtggt                                           >  2:2823691/1‑100 (MQ=255)
                                                       ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtggtg                                          <  2:2345623/100‑1 (MQ=255)
                                                         cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                        <  1:4387068/100‑1 (MQ=255)
                                                         cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                        <  2:39820/100‑1 (MQ=255)
                                                          accccACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcgc                                       <  1:1345842/99‑1 (MQ=255)
                                                           ccccACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg                                      >  2:464097/1‑100 (MQ=255)
                                                           ccccACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg                                      <  1:943915/100‑1 (MQ=255)
                                                              cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa                                   >  1:1053953/1‑100 (MQ=255)
                                                               acccaGTTTTAGTTTCTGACCAACTTCCATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAAt                                  <  2:3512990/100‑1 (MQ=255)
                                                               acccaGTTTTAGTTTCTGACCAACCGCCAAGGTGCAA‑‑GGTGGGGGAAATGACGGTCAGACGCGCCAGTGATTATACGCTGGTTCCCGTGGTGCGCGaaag                                  <  2:3713216/100‑2 (MQ=255)
                                                                cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATAc                                <  1:4013859/100‑1 (MQ=255)
                                                                  caGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACg                               <  1:593908/100‑1 (MQ=255)
                                                                   aGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGa                              >  1:4943611/1‑100 (MQ=255)
                                                                     ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGat                             <  2:4129024/100‑1 (MQ=255)
                                                                     ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGata                            <  1:1738365/100‑1 (MQ=255)
                                                                     ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGata                            >  1:4856109/1‑100 (MQ=255)
                                                                     ttttAGTTTCTGACCAACTTCAATGGAGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGata                            >  2:2829111/1‑100 (MQ=255)
                                                                       ttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGAtata                          <  1:4256041/100‑1 (MQ=255)
                                                                         aGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCg                       >  2:4466796/1‑100 (MQ=255)
                                                                          gTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCg                       <  1:5007945/100‑1 (MQ=255)
                                                                          gTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCg                       <  1:859808/100‑1 (MQ=255)
                                                                            ttCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTa                     <  1:3292037/100‑1 (MQ=255)
                                                                             tCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAt                    <  2:3633802/100‑1 (MQ=255)
                                                                              cTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATc                   <  1:2620354/100‑1 (MQ=255)
                                                                               tGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc                  >  1:5389921/1‑100 (MQ=255)
                                                                                gACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccc                 >  2:5474289/1‑100 (MQ=255)
                                                                                gACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccc                 >  2:5397616/1‑100 (MQ=255)
                                                                                gACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccc                 >  2:2426427/1‑100 (MQ=255)
                                                                                  ccAACTTTAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc               <  1:283113/100‑1 (MQ=255)
                                                                                  ccAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc               >  1:5414656/1‑100 (MQ=255)
                                                                                   cAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccccc              <  2:2517340/100‑1 (MQ=255)
                                                                                   cAACATCAATGGTGTAAGGGGGGGGAAATGCCGTTGAGTCGCGCCAGTTCTTTTACGATGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccccc              >  2:4767888/1‑100 (MQ=255)
                                                                                    aaCTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCg             <  1:1033662/100‑1 (MQ=255)
                                                                                       ttCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGAGATAGCGTATCCCCCCGttt          >  2:4909876/1‑100 (MQ=255)
                                                                                        tCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTGTCCCCCCGTTTc         <  1:4803092/100‑1 (MQ=255)
                                                                                         cAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc       >  2:1396374/1‑100 (MQ=255)
                                                                                          aaTGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc       <  1:83383/100‑1 (MQ=255)
                                                                                          aaTGGTGTAA‑‑GGGGCGGGAAATGCCGTTCAGTCGCGCCCGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGGTATAGCGTATCCCCCCGTTacac       >  2:29117/1‑100 (MQ=255)
                                                                                              gtgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGgtg   <  1:3203492/100‑1 (MQ=255)
                                                                                              gtgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGgtg   <  2:2705408/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
CGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGT  >  NC_000913/2999574‑2999767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: