Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 6,809 T→G K384T (AAA→ACA)  yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009136,8090TG98.9% 638.9 / ‑5.0 181K384T (AAA→ACA) yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (95/84);  total (96/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 181 total aligned reads displayed.

CATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAA  >  NC_000913/6712‑6905
                                                                                                 |                                                                                                
cATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTa                                                                                                >  1:2305488/1‑100 (MQ=255)
  ttATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAgg                                                                                              >  2:1372557/1‑100 (MQ=255)
    aTGATATCTGCCAGTTGCTACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGgt                                                                                            >  2:2458765/1‑100 (MQ=255)
    aTGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGgt                                                                                            >  2:206176/1‑100 (MQ=255)
     tGATATCTGCCAGTTGCCACATCCGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCCGAACGCTGCAgggct                                                                                           >  2:4036843/1‑98 (MQ=255)
         atCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTc                                                                                       <  1:1459132/100‑1 (MQ=255)
          tCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCa                                                                                      >  2:3560997/1‑100 (MQ=255)
          tCTGCCAGTTGCCACAGCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGTAAACACCAGGTCGCTGTAGGGTTGTTCa                                                                                      <  2:4743801/100‑1 (MQ=255)
            tGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAgg                                                                                    <  2:1867226/100‑1 (MQ=255)
             gCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGc                                                                                   <  1:3736056/100‑1 (MQ=255)
             gCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGc                                                                                   >  2:2535861/1‑100 (MQ=255)
             gCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGc                                                                                   >  1:351031/1‑100 (MQ=255)
             gCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGc                                                                                   >  1:14212/1‑100 (MQ=255)
              ccAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCg                                                                                  <  1:4383466/100‑1 (MQ=255)
              ccAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCg                                                                                  <  1:3275964/100‑1 (MQ=255)
                aGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTa                                                                                <  2:1903567/100‑1 (MQ=255)
                   tGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaa                                                                             <  1:28699/100‑1 (MQ=255)
                    gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTaaaag                                                                            >  2:2303484/1‑99 (MQ=255)
                    gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaaa                                                                            >  1:1383077/1‑100 (MQ=255)
                    gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaaa                                                                            >  2:1937218/1‑100 (MQ=255)
                    gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaaa                                                                            >  1:1007912/1‑100 (MQ=255)
                    gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaaa                                                                            >  1:1694763/1‑100 (MQ=255)
                     ccACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaag                                                                           >  2:381834/1‑100 (MQ=255)
                      cacaTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaaga                                                                          >  1:3479415/1‑100 (MQ=255)
                       acaTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaagaa                                                                         <  1:1926910/100‑1 (MQ=255)
                        caTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaagaag                                                                        >  1:5358491/1‑100 (MQ=255)
                        caTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaagaag                                                                        <  2:4970530/100‑1 (MQ=255)
                            aGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAt                                                                    >  1:4557134/1‑100 (MQ=255)
                             gCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAtt                                                                   >  1:4006943/1‑100 (MQ=255)
                             gCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAtt                                                                   <  1:1414451/100‑1 (MQ=255)
                              cGGAAGGCTTAGCAAGGTGTCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTg                                                                  <  2:4919270/100‑1 (MQ=255)
                              cGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTg                                                                  <  1:2542503/100‑1 (MQ=255)
                               ggAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGt                                                                 >  1:4127356/1‑100 (MQ=255)
                               ggAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGt                                                                 >  1:5002461/1‑100 (MQ=255)
                                gAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGtt                                                                <  2:5010155/100‑1 (MQ=255)
                                gAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGtt                                                                <  1:2338513/100‑1 (MQ=255)
                                   ggCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTc                                                             <  1:4909908/100‑1 (MQ=255)
                                   ggCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTc                                                             >  2:3450499/1‑100 (MQ=255)
                                   ggCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTc                                                             >  1:2803496/1‑100 (MQ=255)
                                    gCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCg                                                            >  1:1058633/1‑100 (MQ=255)
                                    gCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCg                                                            >  1:3115496/1‑100 (MQ=255)
                                      ttAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCTGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGc                                                          <  1:462334/100‑1 (MQ=255)
                                      ttAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGc                                                          >  1:3532009/1‑100 (MQ=255)
                                      ttAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGc                                                          >  2:4981537/1‑100 (MQ=255)
                                       tAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCa                                                         >  2:2904301/1‑100 (MQ=255)
                                         gCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATa                                                       >  1:2347830/1‑100 (MQ=255)
                                           aaGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATaaa                                                     <  2:3996897/100‑1 (MQ=255)
                                            aGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAAt                                                    >  1:547162/1‑100 (MQ=255)
                                             ggTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATg                                                   >  2:3281927/1‑100 (MQ=255)
                                              gTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGt                                                  >  2:3055732/1‑100 (MQ=255)
                                               tGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTa                                                 <  2:1998992/100‑1 (MQ=255)
                                                gccgccGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAg                                                >  2:1944366/1‑100 (MQ=255)
                                                gccgccGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAg                                                >  1:3078148/1‑100 (MQ=255)
                                                    ccgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTgg                                            >  1:73147/1‑100 (MQ=255)
                                                    ccgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTgg                                            >  1:4482676/1‑100 (MQ=255)
                                                        tGACCGTCGCGAAGGTGCAGATCCGCAAACATCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCacc                                        <  2:5102600/100‑3 (MQ=255)
                                                          aCCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGa                                      >  1:5574292/1‑100 (MQ=255)
                                                           ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAAcaat                                     >  1:1095910/1‑97 (MQ=255)
                                                           ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     >  1:1636204/1‑100 (MQ=255)
                                                           ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGGAAGAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     >  2:2078080/1‑100 (MQ=255)
                                                            cGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATg                                    >  2:1647447/1‑100 (MQ=255)
                                                               tGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAg                                 <  2:678169/100‑1 (MQ=255)
                                                                 cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  2:1829824/1‑100 (MQ=255)
                                                                 cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  1:771904/1‑100 (MQ=255)
                                                                 cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  1:2680505/1‑100 (MQ=255)
                                                                 cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  1:2457255/1‑100 (MQ=255)
                                                                    aGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTNGTTCAGGCGTAAAAAGAAGAGATTGTTTTNGGCANAAATGTAGTTGGCAACCATGGAGCTGaa                            <  2:2993848/100‑1 (MQ=255)
                                                                     ggTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           <  2:4168379/100‑1 (MQ=255)
                                                                     ggTGCAGAACCGCAAACACCAGATCGCTGTAGGGTAGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           <  2:2772001/100‑1 (MQ=255)
                                                                       tGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGc                         >  2:848968/1‑100 (MQ=255)
                                                                       tGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGc                         <  1:4874407/100‑1 (MQ=255)
                                                                       tGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTGGTCAACGATGGAGCTGAAGGc                         <  1:4753328/100‑1 (MQ=255)
                                                                        gCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCa                        <  1:4265511/100‑1 (MQ=255)
                                                                          aGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCaaa                      >  2:2028561/1‑100 (MQ=255)
                                                                          aGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCaaa                      >  1:1205667/1‑100 (MQ=255)
                                                                           gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     >  2:1267891/1‑100 (MQ=255)
                                                                           gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     <  1:3482838/100‑1 (MQ=255)
                                                                           gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCACGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     >  2:5356382/1‑100 (MQ=255)
                                                                            aTCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACa                    >  1:5144091/1‑100 (MQ=255)
                                                                            aTCCGCAAACACCAGATCGCTGTAGGGTTGGTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACa                    <  2:1225854/100‑1 (MQ=255)
                                                                             tCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAg                   <  1:208179/100‑1 (MQ=255)
                                                                             tCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAg                   >  1:3779714/1‑100 (MQ=255)
                                                                             tCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAg                   <  2:4312815/100‑1 (MQ=255)
                                                                             tCCGCAAACACCAGATCGCTGTAGGGTTGTTAAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAg                   <  2:328676/100‑1 (MQ=255)
                                                                              ccGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGa                  >  2:1434346/1‑100 (MQ=255)
                                                                                gCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAAt                >  2:389708/1‑100 (MQ=255)
                                                                                gCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAAt                <  1:807499/100‑1 (MQ=255)
                                                                                 cAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATa               >  1:2598531/1‑100 (MQ=255)
                                                                                  aaaCACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAGTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATaa              <  1:1551674/100‑1 (MQ=255)
                                                                                     cacCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACca           >  2:1003435/1‑100 (MQ=255)
                                                                                       ccAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcaca         >  2:3971396/1‑100 (MQ=255)
                                                                                       ccAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcaca         >  1:3813275/1‑100 (MQ=255)
                                                                                         aGATCGCTGTAGGGGTGTTCAGGCGTAAAAAGAAGAGATTGGTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAg       <  2:3919080/100‑1 (MQ=255)
                                                                                          gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  2:4215981/1‑100 (MQ=255)
                                                                                          gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  1:4887698/1‑100 (MQ=255)
                                                                                          gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  1:5503187/1‑100 (MQ=255)
                                                                                          gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  1:1592516/1‑100 (MQ=255)
                                                                                          gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGACAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  1:5317593/1‑100 (MQ=255)
                                                                                           aTCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAggg     <  2:2767817/100‑1 (MQ=255)
                                                                                             cGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTa   <  1:4389980/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAA  >  NC_000913/6712‑6905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: