Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,036,444 T→G Y204D (TAT→GAT)  hchA → glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,4440TG98.9% 632.3 / NA 179Y204D (TAT→GAT) hchAglyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (76/100);  minor base C (1/1);  total (77/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

ACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAA  >  NC_000913/2036347‑2036541
                                                                                                 |                                                                                                 
aCAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGAt                                                                                                 <  2:3095762/100‑1 (MQ=255)
  aGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTc                                                                                               <  1:1810553/100‑1 (MQ=255)
   gTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTcc                                                                                              >  1:3577300/1‑100 (MQ=255)
        cAATCAAAAATGACTGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                         <  2:5030104/100‑1 (MQ=255)
        cAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCTGCTTTTCTGGCGCTTCGCTACGGCGATAACCCACTGAATGGTCATTCCATTTg                                                                                         <  2:5071225/100‑1 (MQ=255)
        cAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                         <  1:1263054/100‑1 (MQ=255)
        cAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                         <  1:3556219/100‑1 (MQ=255)
        cAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                         <  2:8080/100‑1 (MQ=255)
        cAATCAAAAATGACCGGTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                         <  2:4465540/100‑1 (MQ=255)
         aaTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgc                                                                                        >  2:4727109/1‑100 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                       <  1:3066634/100‑1 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                       >  2:4718295/1‑100 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCAGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                       >  1:4248677/1‑100 (MQ=255)
           tCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcgc                                                                                      >  1:943208/1‑100 (MQ=255)
           tCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcgc                                                                                      >  2:3911112/1‑100 (MQ=255)
              aaaaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCAtt                                                                                   <  1:3017688/100‑1 (MQ=255)
               aaaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTc                                                                                  <  2:1107936/100‑1 (MQ=255)
               aaaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTc                                                                                  <  2:5331149/100‑1 (MQ=255)
                aaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTcc                                                                                 <  1:4076203/100‑1 (MQ=255)
                 aTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTccc                                                                                <  2:3616907/100‑1 (MQ=255)
                 aTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTccc                                                                                <  2:1674733/100‑1 (MQ=255)
                  tGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCa                                                                               <  1:739749/100‑1 (MQ=255)
                  tGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCa                                                                               <  2:4539947/100‑1 (MQ=255)
                   ggccGTTTTGTTAGCTCCCTTTGCCACGGCCCAGCGGCTTTTCTGGCGTTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAg                                                                              <  2:2534951/98‑1 (MQ=255)
                   gACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAg                                                                              <  1:5391111/100‑1 (MQ=255)
                    aCCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGa                                                                             >  2:3404433/1‑100 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                            <  2:2840088/100‑1 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                            <  1:2844013/100‑1 (MQ=255)
                       gTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAcgc                                                                          >  1:5428748/1‑100 (MQ=255)
                          ttGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAcgccgc                                                                       <  2:2827708/100‑1 (MQ=255)
                           tGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCa                                                                      <  1:4290192/100‑1 (MQ=255)
                           tGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCa                                                                      >  1:4509427/1‑100 (MQ=255)
                             ttATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGa                                                                    <  1:987680/100‑1 (MQ=255)
                              tATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGac                                                                   >  1:2728705/1‑100 (MQ=255)
                              tATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGac                                                                   >  2:1260220/1‑100 (MQ=255)
                                 ctcCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaaa                                                                >  2:1255252/1‑100 (MQ=255)
                                  tcCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaaac                                                               >  1:5552901/1‑100 (MQ=255)
                                   cccTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaaaca                                                              <  2:1444342/100‑1 (MQ=255)
                                   cccTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaaaca                                                              <  2:1844825/100‑1 (MQ=255)
                                      tttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACaaacaaac                                                           <  2:4266766/100‑1 (MQ=255)
                                      tttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACaaacaaac                                                           <  1:696731/100‑1 (MQ=255)
                                         gCCACGGCCCGGCGGATTTTCTGGCGCTTCACCACGGCGATAACCAACTGAATGGTGATTCCATTTGCGCATTCCCGGACGCCGCAGACACACAAACGcc                                                        >  1:4849898/1‑100 (MQ=255)
                                          ccACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCCGACGCCGCAGACAAACAAACGCCa                                                       >  1:1868588/1‑100 (MQ=255)
                                          ccACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCa                                                       >  1:5234215/1‑100 (MQ=255)
                                          ccACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCa                                                       >  2:1398036/1‑100 (MQ=255)
                                           cACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCag                                                      <  1:3117988/100‑1 (MQ=255)
                                           cACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCag                                                      >  1:5408915/1‑100 (MQ=255)
                                            aCGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCaga                                                     >  1:774621/1‑100 (MQ=255)
                                            aCGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCaga                                                     <  1:4176705/100‑1 (MQ=255)
                                            aCGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCaga                                                     <  2:2993972/100‑1 (MQ=255)
                                             cGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCagag                                                    <  2:3442589/100‑1 (MQ=255)
                                             cGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCagag                                                    <  1:874784/100‑1 (MQ=255)
                                               gCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGAt                                                  <  1:5162143/100‑1 (MQ=255)
                                                  cggcggCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGTGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTgg                                               <  2:5174365/100‑1 (MQ=255)
                                                  cggcggCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTgg                                               <  1:5072592/100‑1 (MQ=255)
                                                  cggcggCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTgg                                               <  2:3841764/100‑1 (MQ=255)
                                                   ggcggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGc                                              <  2:2937030/100‑1 (MQ=255)
                                                   ggcggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGc                                              <  2:4998192/100‑1 (MQ=255)
                                                    tcggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCt                                             <  1:1743226/99‑1 (MQ=255)
                                                    gcggcTTTTCTGGCGCTTCGCCACGACGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCt                                             <  2:2334639/100‑1 (MQ=255)
                                                     cggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCta                                            >  2:4786053/1‑100 (MQ=255)
                                                     cggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCta                                            >  2:3425828/1‑100 (MQ=255)
                                                       gcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtata                                          >  2:681547/1‑100 (MQ=255)
                                                       gcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtata                                          >  2:693161/1‑100 (MQ=255)
                                                        cTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTatat                                         <  1:3651745/100‑1 (MQ=255)
                                                            tCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCg                                     <  2:4909810/100‑1 (MQ=255)
                                                             cTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCgg                                    <  2:868122/100‑1 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                   <  1:1495196/100‑1 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                   <  1:1273646/100‑1 (MQ=255)
                                                                gcgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTc                                 <  2:165537/100‑1 (MQ=255)
                                                                 ngcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCa                                >  1:2260262/2‑100 (MQ=255)
                                                                 cgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCa                                <  2:1862238/100‑1 (MQ=255)
                                                                 cgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCa                                <  2:5038786/100‑1 (MQ=255)
                                                                 cgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCa                                <  1:4886881/100‑1 (MQ=255)
                                                                   cTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtc                              <  1:2559922/100‑1 (MQ=255)
                                                                     tCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtctc                            <  2:4617276/100‑1 (MQ=255)
                                                                     tCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtctc                            <  1:1411329/100‑1 (MQ=255)
                                                                      cGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCa                           <  1:5057437/100‑1 (MQ=255)
                                                                      cGCAACGGCGATAACCCACTGAATGGTGATCCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCa                           >  2:2420686/1‑100 (MQ=255)
                                                                       gCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAc                          >  1:1210918/1‑100 (MQ=255)
                                                                        ccACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCGAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                         <  2:4076481/100‑1 (MQ=255)
                                                                        ccACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                         <  1:3820846/100‑1 (MQ=255)
                                                                        ccACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                         <  2:2024804/100‑1 (MQ=255)
                                                                         cACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCt                        >  2:1060014/1‑100 (MQ=255)
                                                                            ggCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGt                     <  1:4689931/100‑1 (MQ=255)
                                                                                aTAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACtt                 <  1:1041891/100‑1 (MQ=255)
                                                                                  aaCCTAATGAATGGTGAATCCATTTGCGCATTCCCAGACGGCGCAGACAAACAAAAGCCAGAGATTGGCTATATGTCGGGGAACCTCACCTATTACTTCg               >  2:172940/1‑100 (MQ=255)
                                                                                   aCCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCgg              >  2:4578814/1‑100 (MQ=255)
                                                                                   aCCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCgg              >  1:3497252/1‑100 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc             <  2:911110/100‑1 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc             >  2:2270018/1‑100 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc             <  2:2653690/100‑1 (MQ=255)
                                                                                     ccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCg            >  1:2922843/1‑100 (MQ=255)
                                                                                      cACGGAATGATGATTCCATTTGCGCATTCCCAGACGCCGCTGACAAACAAACGCAAGAGCTTCGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCga           >  2:1419908/1‑100 (MQ=255)
                                                                                          gAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCgaagaa       >  2:3912088/1‑100 (MQ=255)
                                                                                          gAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACACCAGAGATTTGCTAGATGCCGGGTCATCTCACCTGGTACTTCGGCgcagaa       >  2:4469834/1‑100 (MQ=255)
                                                                                           aaTGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAAc      <  2:2090233/100‑1 (MQ=255)
                                                                                           aaTGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAAc      <  1:3929129/100‑1 (MQ=255)
                                                                                              ggTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTga   >  1:4531326/1‑100 (MQ=255)
                                                                                               gTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTgaa  <  1:2401746/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
ACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAA  >  NC_000913/2036347‑2036541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: