BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,056,867	A→C	K4Q (AAA→CAA)	yeeN →	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,056,867	0	A	C	97.4%	650.1 / 5.0	196	K4Q (AAA→CAA)	yeeN	UPF0082 family protein
Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (106/82); total (111/85)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 198 total aligned reads displayed.

ATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAAC  >  NC_000913/2056770‑2056963

aTTTTTCCTGTTATGATATAATATGTTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTcaa                                                                                                <  2:3314570/100‑1 (MQ=255)

 tttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTcaan                                                                                               <  1:1037056/100‑2 (MQ=255)

 tttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAAt                                                                                               <  2:4192288/100‑1 (MQ=255)

 tttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAAt                                                                                               <  1:3045941/100‑1 (MQ=255)

 tttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAAt                                                                                               <  1:1886678/100‑1 (MQ=255)

  ttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATg                                                                                              <  2:217673/100‑1 (MQ=255)

   tttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATgg                                                                                             <  2:274059/100‑1 (MQ=255)

     tCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTGCCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGc                                                                                           <  2:5174940/100‑1 (MQ=255)

      ccTGTTATGATATAATATGCTGAATTAACACATGTTATCGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGcc                                                                                          <  2:4991824/100‑1 (MQ=255)

      ccTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGcc                                                                                          <  1:3356112/100‑1 (MQ=255)

       cTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCa                                                                                         >  2:1371906/1‑100 (MQ=255)

        tGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCaa                                                                                        >  2:488676/1‑100 (MQ=255)

         gTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAat                                                                                       >  2:3789894/1‑100 (MQ=255)

          ttATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAata                                                                                      <  2:2808748/100‑1 (MQ=255)

           tATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAatat                                                                                     >  2:112404/1‑100 (MQ=255)

           tATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAatat                                                                                     >  2:112571/1‑100 (MQ=255)

            ctggtataGTATGCGGAATTAACACAAGTTAAAGAGTTACCAGTAATGTAAATAAGTTTTACATGAACTCATACCAGTGCGACGGCAATGGGCCAATAtt                                                                                    >  2:2042986/5‑100 (MQ=255)

             tGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttg                                                                                   <  1:4849070/100‑1 (MQ=255)

             tGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttg                                                                                   >  1:673723/1‑100 (MQ=255)

               atatAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttgtt                                                                                 >  2:238917/1‑100 (MQ=255)

               atatAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttgtt                                                                                 <  1:2202148/100‑1 (MQ=255)

                tataATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttgttg                                                                                <  2:3150382/100‑1 (MQ=255)

                tataATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATAttgttg                                                                                <  2:5240749/100‑1 (MQ=255)

                  taataTGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCt                                                                              <  1:2444173/100‑1 (MQ=255)

                   aataTGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTa                                                                             >  1:2370489/1‑100 (MQ=255)

                    atatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaa                                                                            >  2:1307449/1‑100 (MQ=255)

                     tatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaa                                                                           <  1:1652835/100‑1 (MQ=255)

                     tatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaa                                                                           <  2:2346611/100‑1 (MQ=255)

                        gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaaaaa                                                                        >  1:3101827/1‑100 (MQ=255)

                        gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaaaaa                                                                        >  2:3047695/1‑100 (MQ=255)

                        gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaaaaa                                                                        >  1:2348730/1‑100 (MQ=255)

                        gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaaaaa                                                                        >  2:630520/1‑100 (MQ=255)

                         cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTaaaaaaa                                                                       >  1:3411751/1‑100 (MQ=255)

                          tGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAAc                                                                      >  1:2888289/1‑100 (MQ=255)

                          tGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAAc                                                                      >  1:5493286/1‑100 (MQ=255)

                           gAATTAACACATGTTAACGATTTACCAGTAATGTAATTAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCAAATATTGTTGCTAAAAAAACg                                                                     <  2:4008312/100‑1 (MQ=255)

                            aaTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACgg                                                                    <  1:3802468/100‑1 (MQ=255)

                            aaTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACgg                                                                    <  1:3646004/100‑1 (MQ=255)

                            aaTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAAAgg                                                                    >  2:508367/1‑100 (MQ=255)

                            aaTTAACACATGTCAACGATTTACCAGTAATGTAAATAAATTTTCGAGGTGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACgg                                                                    <  1:5023455/100‑1 (MQ=255)

                               tAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTa                                                                 <  1:494193/100‑1 (MQ=255)

                               tAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTa                                                                 <  1:2790115/100‑1 (MQ=255)

                                aaCACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTaa                                                                >  2:5370285/1‑100 (MQ=255)

                                  cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGATGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAg                                                              >  2:1725822/1‑100 (MQ=255)

                                  cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAg                                                              <  2:659829/100‑1 (MQ=255)

                                  cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAg                                                              <  1:1178046/100‑1 (MQ=255)

                                   acaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGa                                                             >  1:4302944/1‑100 (MQ=255)

                                     aTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACg                                                           >  1:4406669/1‑100 (MQ=255)

                                     aTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACg                                                           >  1:3568486/1‑100 (MQ=255)

                                      tGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACgg                                                          <  1:2462956/100‑1 (MQ=255)

                                         tAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGc                                                       <  2:2668288/100‑1 (MQ=255)

                                         naaCGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGc                                                       >  1:4415811/2‑100 (MQ=255)

                                            cGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCACTGGGCCAATATTGTTGCTAAAAAAACAGCTAAAGACGGTGCAAc                                                    >  1:5118328/1‑100 (MQ=255)

                                            cGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAAc                                                    <  1:86078/100‑1 (MQ=255)

                                             gATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACg                                                   >  1:550062/1‑100 (MQ=255)

                                               tttACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTc                                                 <  1:1324947/100‑1 (MQ=255)

                                                ttACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCt                                                <  1:3740746/100‑1 (MQ=255)

                                                 tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTa                                               >  2:1786284/1‑100 (MQ=255)

                                                 tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTa                                               <  1:2291065/100‑1 (MQ=255)

                                                     aGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAAt                                           >  2:1648981/1‑100 (MQ=255)

                                                      gTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAAtt                                          <  2:3519079/100‑1 (MQ=255)

                                                        aaTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTa                                        <  1:2564401/100‑1 (MQ=255)

                                                         aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTAt                                       <  1:2640587/100‑1 (MQ=255)

                                                          tGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATg                                      >  2:9498/1‑100 (MQ=255)

                                                          tGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATg                                      <  1:1278580/100‑1 (MQ=255)

                                                          tGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATg                                      <  1:3198517/100‑1 (MQ=255)

                                                           gTAAATAAATTTTCGAGGAGAGCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAGTACGGCTAAAGACGGTGCAACGTCTAAAATTTATGc                                     <  2:2967180/100‑1 (MQ=255)

                                                            taaataaaTTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCa                                    >  1:1670112/1‑100 (MQ=255)

                                                              aataaatTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaaa                                  >  1:5421388/1‑100 (MQ=255)

                                                               ataaatTTTCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaaaa                                 >  2:2710279/1‑100 (MQ=255)

                                                                    ttttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                            <  2:735770/100‑1 (MQ=255)

                                                                    ttttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                            <  2:4415160/100‑1 (MQ=255)

                                                                    ttttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                            <  1:4913676/100‑1 (MQ=255)

                                                                    ttttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                            <  1:738826/100‑1 (MQ=255)

                                                                     tttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGTAACGTCTAAAATTTATGCAAAATTCGgt                           <  1:1387431/100‑1 (MQ=255)

                                                                      ttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGgtg                          <  2:4374348/100‑1 (MQ=255)

                                                                      ttCGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAAGATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGgtg                          <  1:5321838/100‑1 (MQ=255)

                                                                        cGAGGAGATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTa                        >  2:2642299/1‑100 (MQ=255)

                                                                         gaggagATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAg                       >  2:3134019/1‑100 (MQ=255)

                                                                          aggagATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGa                      <  1:3385997/100‑1 (MQ=255)

                                                                           ggagATCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGaa                     >  1:459175/1‑100 (MQ=255)

                                                                             agaTCATTCCAGTTGGACGTCAATGGGCAAATATTGTTGCTAAAAAAACGGCTAAAGACCGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAAt                   >  1:4907226/1‑100 (MQ=255)

                                                                             agaTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAAt                   >  2:3877753/1‑100 (MQ=255)

                                                                              gaTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATc                  >  1:2842474/1‑100 (MQ=255)

                                                                              gaTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATc                  <  1:113577/100‑1 (MQ=255)

                                                                              gaTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATc                  <  1:5007963/100‑1 (MQ=255)

                                                                              gaTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATc                  >  2:1981319/1‑100 (MQ=255)

                                                                               aTCATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCt                 >  1:5530350/1‑100 (MQ=255)

                                                                                 cATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAt               >  2:2583907/1‑100 (MQ=255)

                                                                                 cATTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAt               >  2:2193274/1‑100 (MQ=255)

                                                                                  aTTCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtg              <  2:3535498/100‑1 (MQ=255)

                                                                                   ttCCAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgc             >  1:4117277/1‑100 (MQ=255)

                                                                                     ccAGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctg           >  2:4607391/1‑100 (MQ=255)

                                                                                       aGTGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgct         <  1:2004826/100‑1 (MQ=255)

                                                                                         tGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctgc       <  2:3356257/100‑1 (MQ=255)

                                                                                         tGGGACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctgc       <  1:1525244/100‑1 (MQ=255)

                                                                                          gggACGTTAATGGGGCAATATTGTTGCTAAAAAAACGGCTAAAGCCGGTGCAACGTTTAAAATTTATGCAAAATTCGGTGTAGAAATCTATgctgctgct      <  2:1228184/100‑1 (MQ=255)

                                                                                            gACGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTaa    >  1:3577558/1‑100 (MQ=255)

                                                                                              cGTCAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAAc  >  2:2462614/1‑100 (MQ=255)

                                                                                              cGTCAATGAGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATATATGCTGCTGCTAAAc  <  2:5470704/100‑1 (MQ=255)

ATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAAC  >  NC_000913/2056770‑2056963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`