Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,112 A→C I161L (ATC→CTC)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,1120AC98.2% 582.1 / ‑4.2 163I161L (ATC→CTC) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (77/83);  total (79/84)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAC  >  NC_000913/2281015‑2281208
                                                                                                 |                                                                                                
ccAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTc                                                                                                <  2:2975519/100‑1 (MQ=255)
 cAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGctct                                                                                               >  1:1685557/1‑100 (MQ=255)
  aGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTa                                                                                              <  2:383502/100‑1 (MQ=255)
   gTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAc                                                                                             >  2:5029450/1‑100 (MQ=255)
    tATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAcc                                                                                            <  1:1153774/100‑1 (MQ=255)
    tATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAcc                                                                                            <  1:170302/100‑1 (MQ=255)
     aTCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCa                                                                                           >  1:4622382/1‑100 (MQ=255)
      tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAg                                                                                          >  1:1889552/1‑100 (MQ=255)
      tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAg                                                                                          <  1:4897358/100‑1 (MQ=255)
      tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAg                                                                                          >  1:5088947/1‑100 (MQ=255)
       cAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGt                                                                                         >  1:1804123/1‑100 (MQ=255)
        aGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGtt                                                                                        >  2:3317040/1‑100 (MQ=255)
         gCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGttt                                                                                       >  1:3484941/1‑100 (MQ=255)
         gCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGttt                                                                                       >  1:389482/1‑100 (MQ=255)
           aaaTCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCa                                                                                     <  2:751222/100‑1 (MQ=255)
            aaTCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCAt                                                                                    <  1:871618/100‑1 (MQ=255)
              tCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTa                                                                                  <  2:1203896/100‑1 (MQ=255)
              tCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTa                                                                                  <  1:1904741/100‑1 (MQ=255)
               ccTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTAt                                                                                 >  2:105771/1‑100 (MQ=255)
                ctcactcaCCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATc                                                                                >  1:3102787/1‑100 (MQ=255)
                 tcgctcacctcacAAAAGTCAATCCCCACTTACCCCAGCGAGGGCTGATTGCCACACCTTTTCGATTGGACAAATGCCGGCTCTACCAGTTTCATTATCa                                                                               <  2:1310221/97‑1 (MQ=255)
                  cactcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCAc                                                                              <  2:6677/100‑1 (MQ=255)
                  cactcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCAc                                                                              <  2:6599/100‑1 (MQ=255)
                   actcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACg                                                                             <  2:2726251/100‑1 (MQ=255)
                    ctcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACgg                                                                            <  2:5429027/100‑1 (MQ=255)
                      cacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAACGGCTGGCTCTACCAGTTTCATTATCCCGGCa                                                                          >  2:4146284/1‑100 (MQ=255)
                      cacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCa                                                                          <  1:3587270/100‑1 (MQ=255)
                      cacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCa                                                                          <  1:2740216/100‑1 (MQ=255)
                       acCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCAt                                                                         <  2:4557438/100‑1 (MQ=255)
                       acCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCAt                                                                         >  2:3852885/1‑100 (MQ=255)
                       acCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCAt                                                                         <  2:4528308/100‑1 (MQ=255)
                        ccTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATg                                                                        >  2:1819204/1‑100 (MQ=255)
                          tGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAATGCTGGCTCTACCAGTTTCATTATCACGGCATGGt                                                                      >  2:4191177/1‑100 (MQ=255)
                          tGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGt                                                                      >  2:3331673/1‑100 (MQ=255)
                          tGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGt                                                                      <  1:3868114/100‑1 (MQ=255)
                           gACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTCCCAGTTTCATTATCACGGCATGGTa                                                                     >  2:1328228/1‑100 (MQ=255)
                           gACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTa                                                                     <  2:1772844/100‑1 (MQ=255)
                               aaaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcgcg                                                                 <  2:4531992/100‑1 (MQ=255)
                               aaaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcgcg                                                                 >  2:1979845/1‑100 (MQ=255)
                               aaaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcgcg                                                                 >  2:4621559/1‑100 (MQ=255)
                                aaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACgcgg                                                                >  2:1885789/1‑100 (MQ=255)
                                 aGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACgcggc                                                               >  2:3645758/1‑100 (MQ=255)
                                 aGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACgcggc                                                               <  1:2836890/100‑1 (MQ=255)
                                     aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAATGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATg                                                           <  1:4229653/100‑1 (MQ=255)
                                     aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATg                                                           <  2:3083329/100‑1 (MQ=255)
                                       tCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgag                                                         <  1:516548/100‑1 (MQ=255)
                                        ccccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgaga                                                        >  2:300151/1‑100 (MQ=255)
                                        ccccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgaga                                                        <  2:2329021/100‑1 (MQ=255)
                                         cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGCTGAGaa                                                       >  2:4703761/1‑100 (MQ=255)
                                         cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaa                                                       <  1:661787/100‑1 (MQ=255)
                                         cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaa                                                       <  1:3679648/100‑1 (MQ=255)
                                         cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaa                                                       <  2:1530369/100‑1 (MQ=255)
                                         cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaa                                                       <  1:1059988/100‑1 (MQ=255)
                                          ccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaaa                                                      >  1:784763/1‑100 (MQ=255)
                                           cACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAg                                                     <  2:1783658/100‑1 (MQ=255)
                                            aCTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc                                                    >  2:4269125/1‑100 (MQ=255)
                                             cTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGcc                                                   >  1:2383024/1‑100 (MQ=255)
                                              ttACGCCGGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGccc                                                  <  1:103016/100‑1 (MQ=255)
                                               tACCCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTGCGCGGCGATGAGAAAGCCCt                                                 >  2:5052332/1‑100 (MQ=255)
                                                aCGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGcccag                                                >  2:1506274/1‑98 (MQ=255)
                                                 cGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCttt                                               >  1:1735518/1‑100 (MQ=255)
                                                   ccggctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTc                                             <  2:4754078/97‑1 (MQ=255)
                                                   ccTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTc                                             >  1:3374120/1‑100 (MQ=255)
                                                    ctgctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTcc                                            >  1:558477/1‑100 (MQ=255)
                                                      gctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCgn                                          <  1:371588/100‑2 (MQ=255)
                                                       ctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTg                                         <  1:3728820/100‑1 (MQ=255)
                                                         ggggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAc                                       <  1:4273484/100‑1 (MQ=255)
                                                         ggggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAc                                       >  1:3832160/1‑100 (MQ=255)
                                                            gCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGc                                    >  1:1957215/1‑100 (MQ=255)
                                                            gCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGc                                    >  2:1870348/1‑100 (MQ=255)
                                                               gACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCAtt                                 >  2:1059778/1‑100 (MQ=255)
                                                                 cTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTa                               <  1:3994022/100‑1 (MQ=255)
                                                                 cTGCCACGCCTTTTCGATCGGGCAAAGGCTGGCTCTACCAGTTTCATTATTACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTa                               <  1:5291394/100‑1 (MQ=255)
                                                                   tccACGCGTTGTCGTTAGGGCAAACGCTGGCTCTACCATGTTCATTATCACGTCATGGTACGCGGCGATCAGAAAGCCCTTTTCCGTGACTCCATTTAtn                             <  2:5489182/99‑2 (MQ=255)
                                                                   gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGCCTGCCTGTATg                             >  1:4153640/1‑100 (MQ=255)
                                                                   gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCAtagatc                             >  1:4139762/1‑95 (MQ=255)
                                                                   gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATg                             <  2:5164955/100‑1 (MQ=255)
                                                                   gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATg                             >  1:4343343/1‑100 (MQ=255)
                                                                     cACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGTAAGCCCTTTTCCGTGACTGCATTTATGAg                           >  1:4782880/1‑100 (MQ=255)
                                                                          cTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGGTGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGcc                      >  1:1402113/1‑100 (MQ=255)
                                                                            tttCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGc                    <  1:1457465/100‑1 (MQ=255)
                                                                            tttCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGc                    >  1:3097983/1‑100 (MQ=255)
                                                                              tCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTg                  <  1:2568255/100‑1 (MQ=255)
                                                                               cGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGc                 >  1:2067784/1‑100 (MQ=255)
                                                                               cGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGc                 <  2:2282839/100‑1 (MQ=255)
                                                                                gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                <  2:6848/100‑1 (MQ=255)
                                                                                gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                <  2:3122784/100‑1 (MQ=255)
                                                                                gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                <  1:2609128/100‑1 (MQ=255)
                                                                                gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                <  1:3888441/100‑1 (MQ=255)
                                                                                gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                >  1:2268123/1‑100 (MQ=255)
                                                                                     ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata           >  2:2500623/1‑100 (MQ=255)
                                                                                     ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata           >  2:5363929/1‑100 (MQ=255)
                                                                                      gCAAAGGCTGGCTCTACCAGTTTCATTGTCGCGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAGTGCATTTATGCGCTGCCGCTGCGTTatat          <  1:1517244/100‑1 (MQ=255)
                                                                                      gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTatat          >  2:2817711/1‑100 (MQ=255)
                                                                                      gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTatat          >  1:2986773/1‑100 (MQ=255)
                                                                                        aaaGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGa        >  2:2056219/1‑100 (MQ=255)
                                                                                        aaaGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGa        >  1:5532984/1‑100 (MQ=255)
                                                                                          aGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGAtt      <  2:3824455/100‑1 (MQ=255)
                                                                                            gCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTaa    >  2:1401298/1‑100 (MQ=255)
                                                                                              tGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAac  >  2:2152513/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAC  >  NC_000913/2281015‑2281208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: