Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,288,707 T→G pseudogene (208/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,288,7070TG99.0% 670.0 / ‑2.8 193pseudogene (208/2525 nt)yejOpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (105/86);  total (107/86)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 194 total aligned reads displayed.

TGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGAT  >  NC_000913/2288610‑2288803
                                                                                                 |                                                                                                
tGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCt                                                                                                <  2:4028525/100‑1 (MQ=255)
 gcgcCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTg                                                                                               >  1:3851408/1‑100 (MQ=255)
  cgcCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTAtgctgc                                                                                              >  2:952177/1‑100 (MQ=255)
   gcCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTgcg                                                                                             >  2:3074050/1‑100 (MQ=255)
    ccATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTgcgg                                                                                            >  2:978294/1‑100 (MQ=255)
    ccATAAAACTGTAGCACACCATTATTGAGCGTAGTGTGGTTTGCGGCGTCACCCCCTTGCACAATATGATAAGCGTCATTTAATACTGAGTATGCTgtgg                                                                                            >  2:5246282/1‑100 (MQ=255)
     cATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTgcggc                                                                                           >  1:1141772/1‑100 (MQ=255)
       tAAAGCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCAGCGCCACACATTTGAACAATTTGATAACGGTGATTTACTACTGCGTATGCTGCGgcgc                                                                                         >  2:5227894/1‑100 (MQ=255)
        aaaCCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGcc                                                                                        >  1:10526/1‑100 (MQ=255)
        aaaCCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGcc                                                                                        <  2:3297769/100‑1 (MQ=255)
         aaCCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCca                                                                                       <  1:2560911/100‑1 (MQ=255)
             tGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCcattca                                                                                   <  1:4034342/100‑1 (MQ=255)
             tGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCcattca                                                                                   <  2:5358435/100‑1 (MQ=255)
              gTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCcattcat                                                                                  >  1:3645976/1‑100 (MQ=255)
                aGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCcattcattn                                                                                <  1:4416168/100‑2 (MQ=255)
                 gCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTc                                                                               <  2:115184/100‑1 (MQ=255)
                 gCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTc                                                                               >  2:3247247/1‑100 (MQ=255)
                   acacCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCaa                                                                             <  1:4780416/100‑1 (MQ=255)
                    cacCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGCTAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAAt                                                                            <  1:4978020/100‑1 (MQ=255)
                    cacCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAAt                                                                            <  2:4487021/100‑1 (MQ=255)
                     acCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATg                                                                           >  2:267034/1‑100 (MQ=255)
                      ccATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGt                                                                          >  2:4985600/1‑100 (MQ=255)
                      ccATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGt                                                                          <  2:1508538/100‑1 (MQ=255)
                      ccATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGt                                                                          >  1:2513749/1‑100 (MQ=255)
                      ccAGTATTGAGCGTAGTCTGGGTAGCCGCGCCACCCATTTGAACAATTTGATGACCGTCAATTAATACTGAGTATGCTGCGGCGCCAGTCAATTCAATGt                                                                          >  2:389306/1‑100 (MQ=255)
                            ttGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGAt                                                                    >  2:2606377/1‑100 (MQ=255)
                             tGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATa                                                                   >  2:2985296/1‑100 (MQ=255)
                              gAGCGTAGTCTGGTTTGGCGCGCCACACATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCAATTCATTTCAATGTACTGATat                                                                  >  2:1043759/1‑99 (MQ=255)
                              gAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATAc                                                                  <  2:4541418/100‑1 (MQ=255)
                              gAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATAc                                                                  >  2:2734302/1‑100 (MQ=255)
                               ngCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATAcc                                                                 >  1:4448318/2‑100 (MQ=255)
                                gCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATAccc                                                                <  1:2079497/100‑1 (MQ=255)
                                gCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATAccc                                                                >  2:4435311/1‑100 (MQ=255)
                                     gTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGt                                                           >  1:4249394/1‑100 (MQ=255)
                                     gTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGt                                                           >  2:86705/1‑100 (MQ=255)
                                     gTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGt                                                           <  1:4300087/100‑1 (MQ=255)
                                      tCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGtt                                                          <  1:2632035/100‑1 (MQ=255)
                                       cTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCACTGTACTGATACCCTCCGTTa                                                         >  1:525494/1‑100 (MQ=255)
                                       cTGGTCTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCTATTCATTTCAATGTACAGATACCCTCCGTTa                                                         >  2:4844839/1‑100 (MQ=255)
                                         ggTTTGCCTCGCCACCCATTTGAACAAGTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAt                                                       <  2:677283/100‑1 (MQ=255)
                                         ggTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAt                                                       >  1:698519/1‑100 (MQ=255)
                                         ggTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAt                                                       >  2:1928402/1‑100 (MQ=255)
                                          gTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAtt                                                      >  1:997752/1‑100 (MQ=255)
                                          gTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAtt                                                      >  2:4878908/1‑100 (MQ=255)
                                           tttGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAAttt                                                     >  1:1083929/1‑100 (MQ=255)
                                            ttGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCGTTTCAATGTACTGATACCCTCCGTTAATTTc                                                    <  1:2725615/100‑1 (MQ=255)
                                            ttGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTc                                                    <  2:4088997/100‑1 (MQ=255)
                                            ttGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTc                                                    <  2:1677608/100‑1 (MQ=255)
                                             tGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCa                                                   <  1:3001210/100‑1 (MQ=255)
                                             tGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCa                                                   >  1:4281605/1‑100 (MQ=255)
                                              gCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAg                                                  <  1:879391/100‑1 (MQ=255)
                                               ccGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGt                                                 <  1:1627380/100‑1 (MQ=255)
                                               ccGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGt                                                 <  2:338488/100‑1 (MQ=255)
                                               ccGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGt                                                 >  2:2693173/1‑100 (MQ=255)
                                               ccGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGt                                                 >  1:4616074/1‑100 (MQ=255)
                                                cgcgCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGta                                                >  1:56033/1‑100 (MQ=255)
                                                cgcgCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGta                                                <  2:1584916/100‑1 (MQ=255)
                                                   gcCACCCATTTGAACAATTTGATCACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCAGTTAATTTCAGtatta                                             >  2:4603345/1‑100 (MQ=255)
                                                   gcCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGtatta                                             >  1:2283398/1‑100 (MQ=255)
                                                    ccACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGtattat                                            <  1:4207722/100‑1 (MQ=255)
                                                    ccACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGATAATTTCAGtattat                                            <  2:4187882/100‑1 (MQ=255)
                                                        ccATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTAttact                                        <  1:3456549/100‑1 (MQ=255)
                                                           tttGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTAttacttac                                     >  2:3305664/1‑100 (MQ=255)
                                                            tttaaCAATTTGATAACCGTCCTTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTCCTTTCAGTATTATTACTTaca                                    >  2:3510038/4‑100 (MQ=255)
                                                            ttGAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTaca                                    <  1:5091361/100‑1 (MQ=255)
                                                              gAACAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACAcc                                  >  2:2397760/1‑100 (MQ=255)
                                                              gAACAATTTGATAACCGTCATTTAATACCGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACAcc                                  <  1:5573593/100‑1 (MQ=255)
                                                              gAACAATTTGATAACCCTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTCCAGTATTATTACTTCCAcc                                  <  2:1346440/100‑1 (MQ=255)
                                                               aaCGATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCa                                 <  2:2476378/100‑1 (MQ=255)
                                                               aaCAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCa                                 >  1:5302353/1‑100 (MQ=255)
                                                               aaCAATTTGATAACCGGCATTTAATAGTGAGTATGCTGCGGCGCCATTCATTTCAATGGACTGATACCCTCCGTTACTTTCATTATTATTACTTACACCa                                 >  2:2512230/1‑100 (MQ=255)
                                                                aCAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCaa                                >  1:3735728/1‑100 (MQ=255)
                                                                 cAATTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCaaa                               >  1:2596257/1‑100 (MQ=255)
                                                                  aaattGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAAt                              <  1:330955/97‑1 (MQ=255)
                                                                   aTTTGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAAtt                             >  1:4495819/1‑100 (MQ=255)
                                                                     ttGATAACCCTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATCCt                           >  1:5434368/1‑100 (MQ=255)
                                                                      tGATAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCtt                          >  1:1792882/1‑100 (MQ=255)
                                                                         tAACCGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTta                       >  1:5155408/1‑100 (MQ=255)
                                                                            ccGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTatat                    >  2:4993409/1‑100 (MQ=255)
                                                                             cGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATg                   >  1:2909278/1‑100 (MQ=255)
                                                                             cGTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATg                   <  1:3213520/100‑1 (MQ=255)
                                                                              gTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGc                  <  2:5090326/100‑1 (MQ=255)
                                                                              gTCATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGc                  <  2:4737682/100‑1 (MQ=255)
                                                                                cATTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTg                <  1:4478857/100‑1 (MQ=255)
                                                                                 aTTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGt               >  1:2175322/1‑100 (MQ=255)
                                                                                 aTTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGt               >  2:2786989/1‑100 (MQ=255)
                                                                                 aTTTAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCCCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGt               >  2:1011389/1‑100 (MQ=255)
                                                                                    tAATACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCACATTCTTTTATATGCTGTtct            <  1:4338776/100‑1 (MQ=255)
                                                                                       tACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCg         <  1:3183875/100‑1 (MQ=255)
                                                                                       tACTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCg         >  2:4247492/1‑100 (MQ=255)
                                                                                         cTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGcc       >  2:2180650/1‑100 (MQ=255)
                                                                                         cTGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGcc       >  2:1437081/1‑100 (MQ=255)
                                                                                          tGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCa      >  2:2136035/1‑100 (MQ=255)
                                                                                          tGAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCa      >  1:1886827/1‑100 (MQ=255)
                                                                                           gAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAg     <  1:613712/100‑1 (MQ=255)
                                                                                           gAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAg     >  1:5407956/1‑100 (MQ=255)
                                                                                           gAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAg     <  2:3816134/100‑1 (MQ=255)
                                                                                           gAGTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAg     <  2:3174912/100‑1 (MQ=255)
                                                                                             gTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAgg  >  1:1727994/1‑99 (MQ=255)
                                                                                             gTATGCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGga   >  1:2189242/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGAT  >  NC_000913/2288610‑2288803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: