Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 301,463 T→G E142A (GAA→GCA)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913301,4630TG99.2% 941.5 / ‑2.6 262E142A (GAA→GCA) paoBPaoABC aldehyde oxidoreductase, FAD‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (144/116);  total (146/116)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 262 total aligned reads displayed.

CGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTG  >  NC_000913/301366‑301559
                                                                                                 |                                                                                                
cGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCa                                                                                                <  1:100181/100‑1 (MQ=255)
cGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCa                                                                                                <  2:3662106/100‑1 (MQ=255)
 gCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCaa                                                                                               >  1:1935762/1‑100 (MQ=255)
 gCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTACTGACGGCTAAAGCCTGCaa                                                                                               >  2:4084245/1‑100 (MQ=255)
  cATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAg                                                                                              <  1:2522328/100‑1 (MQ=255)
   aTCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgc                                                                                             >  2:4157038/1‑100 (MQ=255)
   aTCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgc                                                                                             >  2:3750638/1‑100 (MQ=255)
    tCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcg                                                                                            <  2:4870987/100‑1 (MQ=255)
    tCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcg                                                                                            >  2:4927547/1‑100 (MQ=255)
     ccAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcgc                                                                                           >  2:3373075/1‑100 (MQ=255)
      cAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGcgcc                                                                                          <  1:1393474/100‑1 (MQ=255)
       aGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGcgccg                                                                                         <  1:2779036/100‑1 (MQ=255)
         cAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGGCGGCTAAAGCCTGCAAGCGCcgcg                                                                                       >  2:1350090/1‑100 (MQ=255)
          aaCCGCATTGCGACCGCCATATCACTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCgcgc                                                                                      >  2:2879290/1‑100 (MQ=255)
             cGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGc                                                                                   <  2:3740857/100‑1 (MQ=255)
             cGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGc                                                                                   <  1:558113/100‑1 (MQ=255)
              gCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGcc                                                                                  <  2:2379070/100‑1 (MQ=255)
               cATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCg                                                                                 <  1:2925803/100‑1 (MQ=255)
                    cGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTccc                                                                            <  1:5534968/100‑1 (MQ=255)
                    cGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTccc                                                                            <  2:5076247/100‑1 (MQ=255)
                      aCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCgg                                                                          <  1:1740312/100‑1 (MQ=255)
                       ccGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCggg                                                                         <  2:3530728/100‑1 (MQ=255)
                         gCCATATCGCTCGGATGGATGGCACTGCAGGCTTGGCTTACGCCTGCCACCGCGTGCTGCCGGCTAAAGCCTGCACGCGCCGCGCAGCCGCGCCCGAGCa                                                                       >  2:199235/1‑100 (MQ=255)
                          ccATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAg                                                                      >  2:3244290/1‑100 (MQ=255)
                           cATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAgg                                                                     >  2:3935720/1‑100 (MQ=255)
                            atatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgc                                                                    >  1:987226/1‑100 (MQ=255)
                             tcgtgCTCGGATGGGTGACAGTGAAGGTATCGCTTACGGCTACCACAGCGCGCTGACGGCTAAAGCCTGCAAGCGCCGCGCACCCGCTCCCGGACAGgcg                                                                   >  2:637883/5‑100 (MQ=255)
                             tatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgcg                                                                   >  1:3803985/1‑100 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCTCCGCGTGCTGACGGATAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 <  2:1231845/100‑1 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 >  2:1279716/1‑100 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 <  1:1554274/100‑1 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 <  2:1402967/100‑1 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 >  1:312068/1‑100 (MQ=255)
                                cGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCtt                                                                >  1:2072203/1‑100 (MQ=255)
                                cGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCtt                                                                >  2:798341/1‑100 (MQ=255)
                                  cTCGGATGGGTGACAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCATGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTAt                                                              >  2:5299182/1‑100 (MQ=255)
                                   tCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTAtt                                                             <  2:75123/100‑1 (MQ=255)
                                     ggATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGc                                                           <  1:3190994/100‑1 (MQ=255)
                                        tGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAgg                                                        >  2:2762096/1‑100 (MQ=255)
                                         tggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAggg                                                       <  2:2192090/99‑1 (MQ=255)
                                          ggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAggga                                                      >  1:2214983/1‑99 (MQ=255)
                                          ggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGc                                                      >  1:4913192/1‑100 (MQ=255)
                                           gTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCt                                                     >  1:4604387/1‑100 (MQ=255)
                                             ggCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGa                                                   >  1:1304865/1‑100 (MQ=255)
                                             ggCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGa                                                   >  1:2320583/1‑100 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 >  1:3446806/1‑100 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 >  2:1064770/1‑100 (MQ=255)
                                                aaTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTg                                                <  2:277388/100‑1 (MQ=255)
                                                aaTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTg                                                <  1:1136446/100‑1 (MQ=255)
                                                 aTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTgg                                               <  2:3220176/100‑1 (MQ=255)
                                                 aTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTgg                                               >  1:3032876/1‑100 (MQ=255)
                                                 aTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTgg                                               >  2:2543556/1‑100 (MQ=255)
                                                    cAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGgtgt                                            <  2:4621543/100‑1 (MQ=255)
                                                    cAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGgtgt                                            <  2:3769081/100‑1 (MQ=255)
                                                    cAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGgtgt                                            <  1:4244062/100‑1 (MQ=255)
                                                     aGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTc                                           <  2:1157726/100‑1 (MQ=255)
                                                      ggCTTCGCTTGCGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          >  2:5388776/1‑100 (MQ=255)
                                                      ggCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          <  1:454744/100‑1 (MQ=255)
                                                      ggCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          <  2:3004591/100‑1 (MQ=255)
                                                      ggCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          >  1:3476098/1‑100 (MQ=255)
                                                      ggCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCATGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          >  1:3775710/1‑100 (MQ=255)
                                                        cTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTa                                        >  1:744919/1‑100 (MQ=255)
                                                         ttCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTaa                                       >  1:2801192/1‑100 (MQ=255)
                                                           cGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTaaaa                                     >  1:2157228/1‑100 (MQ=255)
                                                            gCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTagatc                                    >  2:4971976/1‑96 (MQ=255)
                                                            gCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCCCCGCGCAGCCGCTCCCGCGCAGGCGCTCATTGCAGGGCCGACTGGTGTCGTaaaaa                                    >  2:2471638/1‑100 (MQ=255)
                                                             cTTACGCCTACCACCGCGTGCTGACGGCTCAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGAGCTGATTGGTGTCGTAAAAAt                                   >  1:5193614/1‑100 (MQ=255)
                                                             cTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAAt                                   <  1:4141741/100‑1 (MQ=255)
                                                              ttACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATa                                  <  1:1447228/100‑1 (MQ=255)
                                                              atACGCGTTCGACCGCGTGCTGACGGCTAAAGGCGGAAAGCGCCGCGCGGCAGCTCGCGGGCATGCGCTTTTTGCAGGGTTGATTGGTGTCGGAAAAATa                                  <  2:4883615/99‑1 (MQ=255)
                                                                aCGCCTACGACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgg                                >  1:5373462/1‑100 (MQ=255)
                                                                aCGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgg                                <  2:5445215/100‑1 (MQ=255)
                                                                aCGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgg                                <  2:1120124/100‑1 (MQ=255)
                                                                 cGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAggg                               >  1:4372782/1‑100 (MQ=255)
                                                                 cGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAggg                               >  2:2224339/1‑100 (MQ=255)
                                                                 cGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAggg                               <  1:2614070/100‑1 (MQ=255)
                                                                  gCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgggg                              <  1:1029076/100‑1 (MQ=255)
                                                                  gCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgggg                              <  2:156133/100‑1 (MQ=255)
                                                                   ccTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGc                             <  1:2441518/100‑1 (MQ=255)
                                                                   ccTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGc                             <  1:407811/100‑1 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         >  1:3332158/1‑100 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         <  1:1165011/100‑1 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         <  2:4398745/100‑1 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCAGATTGCTGTCGTAAAAATAGGGGCAgcg                         >  1:5070696/1‑100 (MQ=255)
                                                                        caccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcgc                        >  1:3717133/1‑100 (MQ=255)
                                                                         accGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGcgcg                       >  1:4394054/1‑100 (MQ=255)
                                                                          ccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGt                      >  2:920605/1‑100 (MQ=255)
                                                                          ccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGt                      >  1:3623762/1‑100 (MQ=255)
                                                                           cgcgTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTg                     >  1:2695252/1‑100 (MQ=255)
                                                                             cgTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTgcg                   >  1:2960469/1‑100 (MQ=255)
                                                                                gCTGACGGCTAAACCCTGCAAGCGCCGAACAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCATCGCGTGCGCTg                >  1:4903677/1‑100 (MQ=255)
                                                                                  tGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGa              >  1:3099388/1‑100 (MQ=255)
                                                                                   gACGGCTAAAGCCTGCAAGGGCCGCGCAGCCGCGCCCGGGGAGGCGCTTATTGCAGGGCTGATTGGGGTCGTAAAAAGAGGGGCAGCGCGTGCGCTgggg             >  2:831330/1‑98 (MQ=255)
                                                                                   gACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAg             <  2:649776/100‑1 (MQ=255)
                                                                                     cGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCa           <  2:1914498/100‑1 (MQ=255)
                                                                                       gCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGa         >  2:30719/1‑100 (MQ=255)
                                                                                        cTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAt        >  1:1292113/1‑100 (MQ=255)
                                                                                         tAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAtt       >  1:842173/1‑100 (MQ=255)
                                                                                          aaaGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTa      >  2:4549119/1‑100 (MQ=255)
                                                                                              ccTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTg  >  1:4673087/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTG  >  NC_000913/301366‑301559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑