Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,307,348 T→G Q423P (CAA→CCA)  yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,3480TG97.2% 749.1 / 5.2 218Q423P (CAA→CCA) yojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (93/119);  total (96/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 218 total aligned reads displayed.

TTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTT  >  NC_000913/2307251‑2307444
                                                                                                 |                                                                                                
tttCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGc                                                                                                <  1:1281654/100‑1 (MQ=255)
 ttCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCg                                                                                               >  1:3394337/1‑100 (MQ=255)
 ttCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCg                                                                                               >  1:2522629/1‑100 (MQ=255)
  tttGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAGCTCAATCTTATGAGCCATTTTCAGCTCCGCCAGACACTTCTCAACCAGTGGCgg                                                                                              <  2:5475692/98‑1 (MQ=255)
   cTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCggg                                                                                             <  1:855007/100‑1 (MQ=255)
    tGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGt                                                                                            >  2:2878607/1‑100 (MQ=255)
    tGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGt                                                                                            >  2:2531901/1‑100 (MQ=255)
     gCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGtt                                                                                           <  1:3194565/100‑1 (MQ=255)
       ccTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAg                                                                                         >  1:2991726/1‑100 (MQ=255)
        cTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGc                                                                                        <  1:5030325/100‑1 (MQ=255)
         ttttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCg                                                                                       <  1:1722877/100‑1 (MQ=255)
         ttttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCg                                                                                       <  2:2053260/100‑1 (MQ=255)
         ttttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCg                                                                                       >  2:4491410/1‑100 (MQ=255)
         ttttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCAATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCg                                                                                       <  1:5225042/100‑1 (MQ=255)
          tttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCgg                                                                                      <  1:5506489/100‑1 (MQ=255)
           ttGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCggg                                                                                     >  2:64680/1‑100 (MQ=255)
            tGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGt                                                                                    <  1:1171890/100‑1 (MQ=255)
            tGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGt                                                                                    <  1:4256912/100‑1 (MQ=255)
            tGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGt                                                                                    >  1:2466613/1‑100 (MQ=255)
             gATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGtt                                                                                   >  2:4897390/1‑100 (MQ=255)
              ttAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGttt                                                                                  <  1:1222151/99‑1 (MQ=255)
               tAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTa                                                                                 <  2:4408359/100‑1 (MQ=255)
               tAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTa                                                                                 >  1:4857798/1‑100 (MQ=255)
               tAACTTAAGGTTACCAATACGCCCGTTGCTTAACTAAAGCTTATGCGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTa                                                                                 <  2:1625485/100‑1 (MQ=255)
                aaCTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTAc                                                                                <  2:3009378/100‑1 (MQ=255)
                aaCTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTAc                                                                                <  1:3315740/100‑1 (MQ=255)
                  cTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTAccc                                                                              >  1:1399831/1‑100 (MQ=255)
                  cTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTAccc                                                                              >  1:997589/1‑100 (MQ=255)
                     cAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTcc                                                                           <  2:1920200/100‑1 (MQ=255)
                     cAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTcc                                                                           >  1:4399921/1‑100 (MQ=255)
                     cAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTcc                                                                           >  1:4628250/1‑100 (MQ=255)
                      aGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCg                                                                          >  2:3483205/1‑100 (MQ=255)
                        gTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGc                                                                        >  1:641807/1‑100 (MQ=255)
                        gTTAACAATACGCCCGTTGCTGAACGCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTGTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGc                                                                        <  2:5152746/100‑1 (MQ=255)
                          tAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGccc                                                                      >  2:3922859/1‑100 (MQ=255)
                            aCAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCca                                                                    <  2:1900453/100‑1 (MQ=255)
                            aCAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCca                                                                    >  2:1113018/1‑100 (MQ=255)
                              aaTACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCcagc                                                                  <  1:530324/100‑1 (MQ=255)
                               aTACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCcagca                                                                 >  2:1406654/1‑100 (MQ=255)
                                  cGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGtt                                                              >  2:1121326/1‑100 (MQ=255)
                                  cGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGtt                                                              >  1:3224522/1‑100 (MQ=255)
                                   gCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTg                                                             <  2:3939287/100‑1 (MQ=255)
                                     ccGTTGCTTAACTCGAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGAt                                                           <  2:86168/100‑1 (MQ=255)
                                     ccGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGNGGGTTTACCCTCCGGCCCCAGCANTTGAt                                                           >  1:659468/1‑100 (MQ=255)
                                     ccGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGAt                                                           <  2:2446600/100‑1 (MQ=255)
                                     ccGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGAt                                                           <  1:1766045/100‑1 (MQ=255)
                                      cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATc                                                          <  1:2418428/100‑1 (MQ=255)
                                      cGTGGCTTAACTCAAGGTTATGAGCCAGTTTCAGCGGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATc                                                          <  1:409294/100‑1 (MQ=255)
                                        ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaa                                                        <  1:5110874/100‑1 (MQ=255)
                                        ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaa                                                        <  2:38111/100‑1 (MQ=255)
                                        ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaa                                                        <  2:4962609/100‑1 (MQ=255)
                                         tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaaa                                                       >  2:4584833/1‑100 (MQ=255)
                                         tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaaa                                                       <  1:2400878/100‑1 (MQ=255)
                                            ttACCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAg                                                    <  1:3067921/100‑1 (MQ=255)
                                             tAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGc                                                   >  1:1121156/1‑100 (MQ=255)
                                              aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGcc                                                  <  1:4110970/100‑1 (MQ=255)
                                                cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAg                                                <  2:2143539/100‑1 (MQ=255)
                                                   aaGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACa                                             <  2:3757851/100‑1 (MQ=255)
                                                       ttATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCgg                                         <  2:3194462/100‑1 (MQ=255)
                                                        tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGt                                        <  2:3362819/100‑1 (MQ=255)
                                                        tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGt                                        >  2:4105248/1‑100 (MQ=255)
                                                         aTGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTa                                       <  1:655105/100‑1 (MQ=255)
                                                          tGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaa                                      >  1:5006415/1‑100 (MQ=255)
                                                           gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaaa                                     <  2:953138/100‑1 (MQ=255)
                                                           gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaaa                                     <  1:1463140/100‑1 (MQ=255)
                                                            aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAac                                    <  2:1783751/100‑1 (MQ=255)
                                                            aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAac                                    <  1:217631/100‑1 (MQ=255)
                                                             gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAaca                                   >  1:90445/1‑100 (MQ=255)
                                                               cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACt                                 >  1:2331435/1‑100 (MQ=255)
                                                                aTTTTCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTg                                >  1:399853/1‑100 (MQ=255)
                                                                   ttCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCg                             <  2:5042669/100‑1 (MQ=255)
                                                                    tCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGa                            <  1:2647193/100‑1 (MQ=255)
                                                                    tCAGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGa                            >  2:4351912/1‑100 (MQ=255)
                                                                      aGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaa                          <  2:2770986/100‑1 (MQ=255)
                                                                      aGCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaa                          >  2:4695219/1‑100 (MQ=255)
                                                                       gCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                         >  2:4080142/1‑100 (MQ=255)
                                                                       gCTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCACAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                         <  2:4789964/100‑1 (MQ=255)
                                                                        cTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                        >  1:1011273/1‑100 (MQ=255)
                                                                        cTGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                        <  1:106268/100‑1 (MQ=255)
                                                                         tGCGCCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACa                       >  1:1550963/1‑100 (MQ=255)
                                                                          gcgcCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAg                      <  2:4286979/100‑1 (MQ=255)
                                                                           cgcCAGCCACTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGt                     <  1:5155696/100‑1 (MQ=255)
                                                                            gccagccaCTTCTCAACCAGTGGCTGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt                    <  2:4139515/100‑1 (MQ=255)
                                                                               agccaCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                 <  1:3615902/100‑1 (MQ=255)
                                                                                gccaCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                >  1:2818526/1‑100 (MQ=255)
                                                                                gccaCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAACAGTTGATCAAACAACCAGACATCGGCACCCACTGCCGAAAACAGTTTGCg                >  2:3975227/1‑100 (MQ=255)
                                                                                 ccaCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa               >  2:3245899/1‑100 (MQ=255)
                                                                                  caCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt              <  1:4212450/100‑1 (MQ=255)
                                                                                  caCTTCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt              >  1:5163186/1‑100 (MQ=255)
                                                                                     ttCTCAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGaagat           >  2:120315/1‑96 (MQ=255)
                                                                                      tctcAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc          >  2:359156/1‑100 (MQ=255)
                                                                                         cAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc       >  2:2606715/1‑100 (MQ=255)
                                                                                         cAACCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc       >  2:2402710/1‑100 (MQ=255)
                                                                                          aaCCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc      <  1:4142674/100‑1 (MQ=255)
                                                                                           aCCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCg     >  1:2534713/1‑100 (MQ=255)
                                                                                           aCCAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCg     >  1:2087168/1‑100 (MQ=255)
                                                                                            ccAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg    >  1:2769423/1‑100 (MQ=255)
                                                                                            ccAGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg    <  2:1710676/100‑1 (MQ=255)
                                                                                             cAGTGGCGCGTTAGCGGGGTTACCCTCCGGCCCCAGCAGTTGCCCAAACACCCAGACATCGGGAAACCCTGCCCAAAACCGTTTGCGAGAACCTTCCgct   >  2:653444/1‑98 (MQ=255)
                                                                                              aGTGGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgtt  >  1:3754564/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTT  >  NC_000913/2307251‑2307444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑