BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,353	T→G	intergenic (+102/‑94)	atoC → / → atoD	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,353	0	T	G	98.0%	683.0 / 3.0	199	intergenic (+102/‑94)	atoC/atoD	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (2/2); new base G (85/108); total (89/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 200 total aligned reads displayed.

CAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATG  >  NC_000913/2323255‑2323449

cAAAGCGTGCTATGCAGAAATTTGCACAGTGCTCAATTTTCTGCATAGCCGCTCATTCTCCTTACACATCCCCATCCAATTTCTCCCTTCATATTCAAtt                                                                                                 <  2:4429202/100‑1 (MQ=255)

    agcggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGtt                                                                                             >  2:3424781/5‑100 (MQ=18)

     cgtGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            <  1:3813932/98‑1 (MQ=255)

     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            <  2:4363089/100‑1 (MQ=255)

        gCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAAt                                                                                         <  2:186179/100‑1 (MQ=255)

        gCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAAt                                                                                         <  2:3511093/100‑1 (MQ=255)

        gCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAAt                                                                                         >  1:267834/1‑100 (MQ=21)

         cTATGCAGAAATTTGCACAGTGCGCTATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        <  1:1420936/100‑1 (MQ=255)

         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        <  2:3040837/100‑1 (MQ=255)

         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        <  1:3065415/100‑1 (MQ=255)

          tATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATaa                                                                                       <  1:4179061/100‑1 (MQ=255)

          tATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATaa                                                                                       >  2:103193/1‑100 (MQ=255)

          tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATaa                                                                                       >  2:454384/1‑100 (MQ=25)

           aTGCAGAAATTTTCACCGTGCGCAATGTTCTGCATAGCCGCTCATTCTCCTTATAAATCCACAACCAATTTATCCCTTCATATTCAAGTAGTTAAATAAc                                                                                      <  2:1742273/100‑1 (MQ=255)

           aTGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAAc                                                                                      >  1:2221341/1‑100 (MQ=255)

           aTGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAAc                                                                                      >  1:1965514/1‑100 (MQ=25)

            tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACt                                                                                     >  1:749290/1‑100 (MQ=255)

             gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTa                                                                                    <  2:4146170/100‑1 (MQ=255)

             gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTa                                                                                    >  1:3111780/1‑100 (MQ=255)

             gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTa                                                                                    >  2:4776828/1‑100 (MQ=255)

             gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTa                                                                                    >  1:5321826/1‑100 (MQ=25)

              cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaa                                                                                   >  2:5313173/1‑100 (MQ=37)

               aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaaa                                                                                  >  2:92987/1‑100 (MQ=255)

               aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaaa                                                                                  <  1:4743000/100‑1 (MQ=255)

                 aaaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATc                                                                                <  1:3281776/100‑1 (MQ=255)

                  aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATcc                                                                               >  1:2404469/1‑100 (MQ=255)

                     ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaat                                                                            <  1:878305/100‑1 (MQ=255)

                      tGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaata                                                                           <  2:5105808/100‑1 (MQ=255)

                        gacaGTGCGCAGGTTTCTGCATAGCCGCTCGTTCTCCTTATAAATCCCCATCCTATTTATCCCTTCATACTCTAGTAGTTAAATAACTAAATCCaataat                                                                         <  1:4945237/99‑1 (MQ=255)

                        cacaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaataat                                                                         <  1:3616666/100‑1 (MQ=255)

                           aGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAAtctc                                                                      <  1:4062269/100‑1 (MQ=255)

                               cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTc                                                                  <  2:2770737/100‑1 (MQ=255)

                               cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTc                                                                  <  1:1472191/100‑1 (MQ=255)

                                gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCt                                                                 >  2:4752327/1‑100 (MQ=255)

                                   aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                                              <  1:231218/100‑1 (MQ=255)

                                   aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                                              <  1:3418373/100‑1 (MQ=255)

                                    ttttCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCCGGCa                                                             <  2:4672583/100‑1 (MQ=255)

                                     tttCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCAc                                                            <  2:1292536/100‑1 (MQ=255)

                                         tGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTccc                                                        <  1:2583751/100‑1 (MQ=255)

                                          gCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTcccc                                                       <  1:1901872/100‑1 (MQ=255)

                                          gCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTcccc                                                       >  2:2155753/1‑100 (MQ=255)

                                             tAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTg                                                    <  1:3567803/100‑1 (MQ=255)

                                             tAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTg                                                    >  2:1135384/1‑100 (MQ=255)

                                              aGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGc                                                   >  2:3669662/1‑100 (MQ=255)

                                               gCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCt                                                  <  2:208321/100‑1 (MQ=255)

                                                  gCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                                               <  2:1557370/100‑1 (MQ=255)

                                                  gCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                                               >  2:3499585/1‑100 (MQ=255)

                                                     cATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                                            <  2:1537441/100‑1 (MQ=255)

                                                      aTTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCt                                           >  2:3618076/1‑100 (MQ=255)

                                                      aTTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCt                                           <  2:1644811/100‑1 (MQ=255)

                                                       ttCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTg                                          <  1:1384513/100‑1 (MQ=255)

                                                        tctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                                         >  2:4790729/1‑100 (MQ=255)

                                                        tctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                                         >  2:4698918/1‑100 (MQ=255)

                                                        tctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                                         >  1:3963214/1‑100 (MQ=255)

                                                         ctcCTTATAAATCCCCATCCTATTTATCCCTTCATATTCAAATAGGTAAATAACTAAATCCAGTAATCTCATGCGGGCACTCCCCTTGCTATTTCTTGAc                                        >  2:1365579/1‑100 (MQ=255)

                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  1:4670841/100‑1 (MQ=255)

                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  1:3678962/100‑1 (MQ=255)

                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        >  1:4065890/1‑100 (MQ=255)

                                                          tcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACt                                       >  2:2567648/1‑100 (MQ=255)

                                                           ccTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg                                      >  2:385554/1‑100 (MQ=255)

                                                           ccTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg                                      <  2:2969072/100‑1 (MQ=255)

                                                             ttATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTa                                    >  1:4827170/1‑100 (MQ=255)

                                                              tataATTCCCCATCAAATTTACGCATTAATATTCAACTAGTTAATTAACTAAATCCAATAATCTCATTCTGGTACTCCCCCTGTTATTGCCTGACTGTAc                                   >  2:547129/1‑100 (MQ=255)

                                                              tataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                   <  2:834652/100‑1 (MQ=255)

                                                              tataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                   >  1:4861987/1‑100 (MQ=255)

                                                               ataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAcc                                  >  2:1581583/1‑100 (MQ=255)

                                                                taAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc                                 >  2:1400418/1‑100 (MQ=255)

                                                                taAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc                                 <  2:2352510/100‑1 (MQ=255)

                                                                taAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTCTAccc                                 <  2:3693413/100‑1 (MQ=255)

                                                                 agaTCCCCATCCAATTTATCCCTTGATATTCAAGTAGTTAAATAACTAAATCCGATAGTCTCATTCTGGCACTCCTCTTGCTATTGCGTTACTGTTcccc                                <  2:3790111/98‑2 (MQ=255)

                                                                 aaaTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca                                >  2:4875549/1‑100 (MQ=255)

                                                                 aaaTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca                                <  1:3016065/100‑1 (MQ=255)

                                                                 aaaTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca                                >  1:424308/1‑100 (MQ=255)

                                                                  aaTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcac                               <  1:1254145/100‑1 (MQ=255)

                                                                  aaTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcac                               >  1:2276378/1‑100 (MQ=255)

                                                                   aTCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcaca                              <  2:2666846/100‑1 (MQ=255)

                                                                    tCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                             >  2:1752680/1‑100 (MQ=255)

                                                                    tCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                             >  1:1115655/1‑100 (MQ=255)

                                                                     ccccATCCAATTTATGCCTTCATATTCAAGTAGTTAAATAAGTATATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACACACAAc                            <  2:5242569/100‑1 (MQ=255)

                                                                      cccATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACg                           >  2:4507401/1‑100 (MQ=255)

                                                                      cccATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACg                           >  2:1422176/1‑100 (MQ=255)

                                                                        cATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgt                         <  1:969019/100‑1 (MQ=255)

                                                                           ccAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTa                      <  1:822684/100‑1 (MQ=255)

                                                                           ccAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTa                      <  2:2380524/100‑1 (MQ=255)

                                                                            cAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTAt                     >  1:3464457/1‑100 (MQ=255)

                                                                             aaTTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATg                    <  1:2496334/100‑1 (MQ=255)

                                                                              aTTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGc                   <  2:3567184/100‑1 (MQ=255)

                                                                               cttATCCCTTCATATTCAAGTAGTTCAATCACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCa                  <  2:1232611/99‑1 (MQ=255)

                                                                                  aTCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAaga               <  1:5105764/100‑1 (MQ=255)

                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg            >  1:4481578/1‑100 (MQ=255)

                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg            <  1:3166912/100‑1 (MQ=255)

                                                                                      cTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGa           >  2:685567/1‑100 (MQ=255)

                                                                                      cTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGa           >  1:1662518/1‑100 (MQ=255)

                                                                                       ttCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGAt          >  1:2797804/1‑100 (MQ=255)

                                                                                        tCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATa         <  2:2368668/100‑1 (MQ=255)

                                                                                           tatTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa      >  1:5149561/1‑100 (MQ=255)

                                                                                            atTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaaa     <  2:2239172/100‑1 (MQ=255)

                                                                                            atTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaaa     <  2:739740/100‑1 (MQ=255)

                                                                                              tCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAAt   <  2:3974462/100‑1 (MQ=255)

                                                                                              tCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACCGTACCCACAACGGTCTATGCAAGAGGGATAAAAAAt   >  2:5200920/1‑100 (MQ=255)

CAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATG  >  NC_000913/2323255‑2323449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`