Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,411,354 A→C intergenic (‑2/+85) yfbS ← / ← yfbT putative transporter/sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,3540AC98.7% 799.5 / ‑2.2 228intergenic (‑2/+85)yfbS/yfbTputative transporter/sugar phosphatas
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (102/122);  total (104/124)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

AAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAA  >  NC_000913/2411257‑2411449
                                                                                                 |                                                                                               
 aacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCtt                                                                                              >  2:207852/1‑100 (MQ=255)
 aacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCtt                                                                                              >  1:2972921/1‑100 (MQ=255)
    aaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATc                                                                                           <  1:4817301/100‑1 (MQ=255)
      aGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCt                                                                                         <  1:2625752/100‑1 (MQ=255)
         gACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGt                                                                                      <  2:3769424/100‑1 (MQ=255)
          aCCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGtt                                                                                     >  2:3862138/1‑100 (MQ=255)
           ccGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTa                                                                                    <  2:5069779/100‑1 (MQ=255)
             gcatgcatACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAAt                                                                                  >  2:4682568/1‑100 (MQ=255)
             gCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAAt                                                                                  >  1:5417912/1‑100 (MQ=255)
             gCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAAt                                                                                  <  1:2772864/100‑1 (MQ=255)
             gCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAAt                                                                                  <  2:1413201/100‑1 (MQ=255)
                tCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGa                                                                               >  2:447463/1‑100 (MQ=255)
                 ccATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAg                                                                              <  1:3488041/100‑1 (MQ=255)
                  cATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAgg                                                                             <  1:3864937/100‑1 (MQ=255)
                   aTACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAggg                                                                            <  2:2886291/100‑1 (MQ=255)
                   aTACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAggg                                                                            <  1:2655092/100‑1 (MQ=255)
                   aTACGCACTCTGCCCGTCGCAAACAAGACAATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAggg                                                                            <  2:3606518/100‑1 (MQ=255)
                    tACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAgggg                                                                           <  2:3532830/100‑1 (MQ=255)
                       gCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGAt                                                                        >  1:5379219/1‑100 (MQ=255)
                       gCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGAt                                                                        <  1:2120334/100‑1 (MQ=255)
                        cACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATg                                                                       <  2:5113730/100‑1 (MQ=255)
                         aCTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGa                                                                      >  2:3906576/1‑100 (MQ=255)
                         aCTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGa                                                                      <  1:1960512/100‑1 (MQ=255)
                          ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGAc                                                                     <  2:4177843/100‑1 (MQ=255)
                          ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGAc                                                                     >  2:1501242/1‑100 (MQ=255)
                           tctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACt                                                                    <  2:5332146/100‑1 (MQ=255)
                            ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACtt                                                                   >  2:853443/1‑100 (MQ=255)
                             tGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTg                                                                  >  1:3682/1‑100 (MQ=255)
                               cccGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                                <  1:5026004/100‑1 (MQ=255)
                               cccGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                                >  1:1344660/1‑100 (MQ=255)
                               cccGTCGCAAACAAGACGAGGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                                <  1:2194116/100‑1 (MQ=255)
                                ccGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                               >  1:2861260/1‑100 (MQ=255)
                                    cGCAAACAAGACGATGGCGACGGCGAGTAATGAAAGAACCGAAATCAGTTGTGCGTGCACATCTTAGCCTTGTTAATTGCGGGGGATGACTTGATTCTGc                                                           <  2:1031629/100‑1 (MQ=255)
                                    cGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                                                           <  2:65794/100‑1 (MQ=255)
                                    cGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                                                           <  1:2540078/100‑1 (MQ=255)
                                     gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGcc                                                          >  1:1967412/1‑100 (MQ=255)
                                     gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGcc                                                          >  1:3706215/1‑100 (MQ=255)
                                     gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGcc                                                          >  1:1210347/1‑100 (MQ=255)
                                     gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGcc                                                          <  2:4305866/100‑1 (MQ=255)
                                      cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                                                         >  2:3075670/1‑100 (MQ=255)
                                      cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                                                         <  2:257124/100‑1 (MQ=255)
                                      cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                                                         >  1:2726094/1‑100 (MQ=255)
                                       aaaCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCGATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCAt                                                        <  2:4977463/100‑1 (MQ=255)
                                         aCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                                      >  1:4929335/1‑100 (MQ=255)
                                          cAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaa                                                     <  2:2918391/100‑1 (MQ=255)
                                              aCGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                                 <  1:4746479/100‑1 (MQ=255)
                                                  tGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcccc                                             <  1:1842561/100‑1 (MQ=255)
                                                  tGGCAAAGGTAAGTCATGCAAGAAACGAACTAAATTAACCGTTCACACTTTATAATTGTTAATTGTGGGGGATGATTTGATTTTGCCATAAAAAAGcccc                                             >  2:1104214/1‑100 (MQ=255)
                                                    gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTTAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcga                                           >  2:2284505/1‑100 (MQ=255)
                                                    gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcga                                           >  2:2348560/1‑100 (MQ=255)
                                                     cAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac                                          >  1:1763484/1‑100 (MQ=255)
                                                      aaCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                                         <  2:1836020/100‑1 (MQ=255)
                                                        cGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                                       >  2:5089633/1‑100 (MQ=255)
                                                           ccAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                                    >  1:757599/1‑100 (MQ=255)
                                                             aGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTGATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                                  <  1:3840039/100‑1 (MQ=255)
                                                              gTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                                 >  1:2221138/1‑100 (MQ=255)
                                                               tAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGc                                <  2:1743494/100‑1 (MQ=255)
                                                                aaTGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCATGTTCATTGAGGGGGATGACCTGATTCTGCCCTAAAAACGCCCCGACGAGACGGGGCt                               >  2:5228933/1‑100 (MQ=255)
                                                                 aTGAAAGAACCCAAAGCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                              <  2:1991527/100‑1 (MQ=255)
                                                                  tGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaa                             >  1:3575914/1‑100 (MQ=255)
                                                                  tGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaa                             >  2:5482367/1‑100 (MQ=255)
                                                                   gAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                            >  2:2176543/1‑100 (MQ=255)
                                                                    aaaGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAt                           >  1:1422094/1‑100 (MQ=255)
                                                                       gAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAt                        <  2:1592657/100‑1 (MQ=255)
                                                                       gAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAt                        <  1:1051016/100‑1 (MQ=255)
                                                                        aaCCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg                       <  1:1788090/100‑1 (MQ=255)
                                                                         aCCCAAATCAATTCACCGTTCACACCTCATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGCGACGGGGCTAAATCATGa                      >  2:5463868/1‑100 (MQ=255)
                                                                          cccAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                     >  2:586493/1‑100 (MQ=255)
                                                                          cccAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                     >  1:2096388/1‑100 (MQ=255)
                                                                          cccAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                     <  2:711854/100‑1 (MQ=255)
                                                                          cccAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                     >  2:3279055/1‑100 (MQ=255)
                                                                          accAAATCAATTCACCGTTCACACCTTATCCGTGGTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                     <  2:1268582/99‑1 (MQ=255)
                                                                           ccAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc                    >  2:2977936/1‑100 (MQ=255)
                                                                             aaaTCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAg                  >  1:925596/1‑100 (MQ=255)
                                                                              aaTCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAGAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                 >  2:5413336/1‑100 (MQ=255)
                                                                              aaTCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                 <  2:1928049/100‑1 (MQ=255)
                                                                               aTCAATTCACCGTTCACACCTTCTCCTTGATAATTGAGGGGGATGACATGCTTCTGCCATAAAAAAGACCCGACGAGACGGGGCTAAATCATGTTTAGgt                >  2:5381770/1‑100 (MQ=255)
                                                                               aTCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgt                <  1:3304826/100‑1 (MQ=255)
                                                                                  aaTTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt             >  1:303077/1‑100 (MQ=255)
                                                                                  aaTTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt             <  2:1194220/100‑1 (MQ=255)
                                                                                    ttCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc           <  1:1520427/100‑1 (MQ=255)
                                                                                     tCACCGTTCACACCTTATCCATGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTCAATCATGATCAGGTGTTTCa          >  1:4345867/1‑100 (MQ=255)
                                                                                      cACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCAc         <  1:1116541/100‑1 (MQ=255)
                                                                                      cACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCAc         <  1:3193655/100‑1 (MQ=255)
                                                                                      cACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCAc         >  2:434028/1‑100 (MQ=255)
                                                                                      cACCCTTCAAACCATAGCCTCGGTAATTGAGGGGGATGACTTGATCCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCAc         <  2:3663467/100‑1 (MQ=255)
                                                                                       aCCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACt        >  1:4710088/1‑100 (MQ=255)
                                                                                        ccGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg       <  1:5486681/100‑1 (MQ=255)
                                                                                         cGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGa      >  1:449615/1‑100 (MQ=255)
                                                                                          gTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaa     <  2:5172356/100‑1 (MQ=255)
                                                                                          gTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaa     >  2:3921416/1‑100 (MQ=255)
                                                                                          gTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaa     >  1:1180428/1‑100 (MQ=255)
                                                                                          gTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGGTTCACTGaa     >  2:3455430/1‑100 (MQ=255)
                                                                                           ttCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat    <  1:419546/100‑1 (MQ=255)
                                                                                           ttCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat    <  2:4850882/100‑1 (MQ=255)
                                                                                           ttCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat    <  1:863098/100‑1 (MQ=255)
                                                                                           ttCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat    <  2:402451/100‑1 (MQ=255)
                                                                                           ttCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat    <  2:2699915/100‑1 (MQ=255)
                                                                                            tCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaata   <  1:1493785/100‑1 (MQ=255)
                                                                                             cacaCCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaataa  >  2:2450560/1‑100 (MQ=255)
                                                                                                 |                                                                                               
AAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAA  >  NC_000913/2411257‑2411449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: