Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,420,409 A→C L26W (TTG→TGG)  yfcF ← glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,420,4090AC98.4% 632.5 / 0.4 185L26W (TTG→TGG) yfcFglutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base C (92/88);  total (94/91)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 186 total aligned reads displayed.

CGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCAACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATAAAGG  >  NC_000913/2420313‑2420505
                                                                                                |                                                                                                
cGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACg                                                                                               <  1:755087/100‑1 (MQ=255)
  gCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGcc                                                                                             >  1:337018/1‑100 (MQ=255)
    gtgtCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTgccacgccac                                                                                           >  2:3884145/1‑100 (MQ=255)
    gtgtCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCATGCCAc                                                                                           >  2:3722511/1‑100 (MQ=255)
     tgtCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCAcc                                                                                          <  2:2982872/100‑1 (MQ=255)
      gtCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCAccc                                                                                         >  2:3768131/1‑100 (MQ=255)
       tCTGACCGTAACCTTGCCCCGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCa                                                                                        >  2:4002680/1‑100 (MQ=255)
       tCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCa                                                                                        >  2:3129063/1‑100 (MQ=255)
       tCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCa                                                                                        >  2:2277642/1‑100 (MQ=255)
          gACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGc                                                                                     >  2:4440763/1‑100 (MQ=255)
          gACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGc                                                                                     <  2:5274660/100‑1 (MQ=255)
           aCCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCg                                                                                    >  2:1713998/1‑100 (MQ=255)
            ccGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCgg                                                                                   >  1:4276287/1‑100 (MQ=255)
            ccGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCgg                                                                                   >  1:5018204/1‑100 (MQ=255)
             cGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGa                                                                                  >  2:209164/1‑100 (MQ=255)
              gTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGAt                                                                                 <  1:2868318/100‑1 (MQ=255)
               tAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATa                                                                                <  1:2914362/100‑1 (MQ=255)
               tAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATa                                                                                >  1:1053028/1‑100 (MQ=255)
                aaCCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATaa                                                                               >  1:4707895/1‑100 (MQ=255)
                 aCCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTATTCCTGCCACGCCACCCAGGCGGATAac                                                                              <  1:4916443/100‑1 (MQ=255)
                 aCCTTGCCACGTCGGCTGCAAATGTTCACCGCAGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAac                                                                              <  2:843200/100‑1 (MQ=255)
                  ccTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAaca                                                                             >  1:3211858/1‑100 (MQ=255)
                  ccTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAaca                                                                             >  2:1165432/1‑100 (MQ=255)
                  ccTTGCCACGTCCGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAaca                                                                             <  1:4584657/100‑1 (MQ=255)
                   cTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTTATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAacac                                                                            <  2:4948130/100‑1 (MQ=255)
                      gCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATa                                                                         <  2:3808530/100‑1 (MQ=255)
                      gCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATa                                                                         >  2:3784807/1‑100 (MQ=255)
                       ccACGTCGGCTGGAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATaa                                                                        >  1:4786117/1‑100 (MQ=255)
                        cACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACTCATAAg                                                                       >  1:2900229/1‑100 (MQ=255)
                        cACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAg                                                                       <  2:1871802/100‑1 (MQ=255)
                          cGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAggg                                                                     <  1:140466/100‑1 (MQ=255)
                          cGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAggg                                                                     <  2:505789/100‑1 (MQ=255)
                           gTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAgggg                                                                    >  1:2013606/1‑100 (MQ=255)
                           gTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAgggg                                                                    <  1:12338/100‑1 (MQ=255)
                           gTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAgggg                                                                    <  1:5356182/100‑1 (MQ=255)
                           gTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAgggg                                                                    <  2:2788621/100‑1 (MQ=255)
                             ctgCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGgag                                                                  <  1:5089901/98‑1 (MQ=255)
                              ggCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGgaga                                                                 >  2:3685394/1‑100 (MQ=255)
                               gCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGaa                                                                >  1:1674198/1‑100 (MQ=255)
                                 tGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGTCGGATAACACATAAGGGGAGaaaa                                                              >  2:4136624/1‑100 (MQ=255)
                                 tGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGaaaa                                                              <  1:3819837/100‑1 (MQ=255)
                                    aaaTGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGt                                                           <  1:3047687/100‑1 (MQ=255)
                                      aTGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTgg                                                         <  1:1005641/100‑1 (MQ=255)
                                      aTGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTgg                                                         <  1:211198/100‑1 (MQ=255)
                                       tGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTggg                                                        <  1:3398247/100‑1 (MQ=255)
                                       tGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTggg                                                        >  1:4470611/1‑100 (MQ=255)
                                        gataaCCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAAAAAAAAAAAAAAAAAAAAGTGGGc                                                       <  2:2239975/96‑1 (MQ=255)
                                        gTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGc                                                       >  1:944140/1‑100 (MQ=255)
                                         ttCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCa                                                      <  2:416646/100‑1 (MQ=255)
                                           ctccGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATc                                                    <  1:832921/98‑1 (MQ=255)
                                           cACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATc                                                    >  2:3945227/1‑100 (MQ=255)
                                           cACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATc                                                    <  1:612983/100‑1 (MQ=255)
                                           cACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATc                                                    >  1:3388778/1‑100 (MQ=255)
                                           cACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGAATc                                                    >  2:696339/1‑100 (MQ=255)
                                             ccGCTGTCGAGGTCGATGGTCTTGGTATGAAACGACAGGCCTTTTTCCTGCCACACCACCCAGGCGGATAACAAATAAGTGGAGAAAAAGTGGGCATCTg                                                  >  1:5205022/1‑100 (MQ=255)
                                             ccGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCAACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTg                                                  <  1:1001071/100‑1 (MQ=255)
                                               gCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGAc                                                >  1:2998889/1‑100 (MQ=255)
                                                cTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGAcc                                               <  1:3803030/100‑1 (MQ=255)
                                                 tGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCa                                              <  2:5385473/100‑1 (MQ=255)
                                                  gTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCaa                                             >  1:688531/1‑100 (MQ=255)
                                                    cGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAg                                           >  1:4534971/1‑100 (MQ=255)
                                                    cGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAg                                           >  1:1933494/1‑100 (MQ=255)
                                                       ggTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCATCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGt                                        <  1:2728364/100‑1 (MQ=255)
                                                       ggTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGt                                        <  2:4948419/100‑1 (MQ=255)
                                                       ggTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGt                                        >  1:4034816/1‑100 (MQ=255)
                                                          cGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGAt                                     >  1:3800012/1‑100 (MQ=255)
                                                          cGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGAt                                     <  1:2677486/100‑1 (MQ=255)
                                                           gATGGTCTTGATATGAAACGACAGGCCTTTTTTTTTTTTTTTTACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATa                                    >  2:258527/1‑100 (MQ=255)
                                                           gATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATa                                    <  1:345256/100‑1 (MQ=255)
                                                            aTGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACAAAAAAAAAAAAAAAAAGTGGGCATCTGACCAAAGCGTGATAg                                   <  2:111733/100‑1 (MQ=255)
                                                              ggccTTGATATGAAACGACAGGCTTTTTTTCTGCCACGCAACCCAGGCCGAGAACACATAAGGGCAGAAAAAGTGGGCCTCTGTCTAAAGCGGGATcgcg                                 >  2:1239577/4‑100 (MQ=255)
                                                              ggTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCg                                 >  2:432507/1‑100 (MQ=255)
                                                              ggTCTTGATATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCg                                 <  1:3648851/100‑1 (MQ=255)
                                                              ggTCTTGAGATGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCg                                 <  1:2235302/100‑1 (MQ=255)
                                                              ggTCGTGATAGGAAACGACAGGCCTATTTTGTGACACGGTACCCAGGCGGATAACAGAAAAAAAAAAAAAAAGTGGGCATCTGACCAAAGCGTGATAGCg                                 <  2:4425844/100‑1 (MQ=255)
                                                                      tatGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACt                         >  2:3483953/1‑100 (MQ=255)
                                                                       atGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTc                        <  2:1039088/100‑1 (MQ=255)
                                                                       atGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTc                        <  2:1372481/100‑1 (MQ=255)
                                                                        tGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCa                       <  1:3924475/100‑1 (MQ=255)
                                                                        tGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCa                       >  1:5546614/1‑100 (MQ=255)
                                                                        tGAAACGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCa                       >  1:4218402/1‑100 (MQ=255)
                                                                         gAAACGACAGGCCTTTTTCCTGCCACGCCACCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCAtt                     <  2:2745824/100‑1 (MQ=255)
                                                                          aaaCGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCAtt                     <  2:2838393/100‑1 (MQ=255)
                                                                          aaaCGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAAAGAAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCAtt                     <  2:293746/100‑1 (MQ=255)
                                                                           aaCGACAGGCCTTTTTCCTGCCACGCCACCCAGGCGTATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTa                    <  1:5249733/100‑1 (MQ=255)
                                                                           aaCGACAGGCCTTTTTCCTGCCACACCAACCAAGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTa                    <  2:2133894/100‑1 (MQ=255)
                                                                           aaCGACACGCCGTGTTCCTGCCACGCCAGCCAAGCGGAGAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTa                    <  2:4887090/100‑1 (MQ=255)
                                                                                cAGGCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTcc               >  1:5153645/1‑100 (MQ=255)
                                                                                  ggCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTc             >  1:4737217/1‑100 (MQ=255)
                                                                                   gCCTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCg            >  2:3367366/1‑100 (MQ=255)
                                                                                     cTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGc          <  1:3259507/100‑1 (MQ=255)
                                                                                     cTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGc          >  2:2913963/1‑100 (MQ=255)
                                                                                     cTTTTTCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGc          >  1:4094621/1‑100 (MQ=255)
                                                                                         ttCCTGCCACGCCACCCAGTCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATa      <  1:4174279/100‑1 (MQ=255)
                                                                                         ttCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATa      <  1:4511788/100‑1 (MQ=255)
                                                                                          tCCTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATaa     >  2:5361641/1‑100 (MQ=255)
                                                                                           ccTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATaaa    >  2:3965616/1‑100 (MQ=255)
                                                                                            cTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATAAAg   <  2:4481378/100‑1 (MQ=255)
                                                                                            cTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATAAAg   >  1:1973473/1‑100 (MQ=255)
                                                                                            cTGCCACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATAAAg   >  2:1473903/1‑100 (MQ=255)
                                                                                                |                                                                                                
CGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTTCACCGCTGTCGAGGTCGATGGTCTTGATATGAAACGACAGGCCTTTTTCCTGCAACGCCACCCAGGCGGATAACACATAAGGGGAGAAAAAGTGGGCATCTGACCAAAGCGTGATAGCGGGTTTACTCATTATGTCCTCGGCAATAAAGG  >  NC_000913/2420313‑2420505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: