Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,575,120 T→G K113T (AAG→ACG)  eutP ← putative P‑loop NTPase ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,575,1200TG98.1% 744.8 / ‑1.9 212K113T (AAG→ACG) eutPputative P‑loop NTPase ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (93/115);  total (96/116)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

AGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCGGCAG  >  NC_000913/2575024‑2575213
                                                                                                |                                                                                             
    aTGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGt                                                                                        >  1:4098846/1‑100 (MQ=255)
    aTGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGt                                                                                        >  1:2951081/1‑100 (MQ=255)
     tGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGtt                                                                                       <  1:4869071/100‑1 (MQ=255)
     tGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGtt                                                                                       >  1:1026117/1‑100 (MQ=255)
      gCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCCAGCAATAACGTTCGCGTTg                                                                                      >  2:2937078/1‑100 (MQ=255)
       ccAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgc                                                                                     >  1:1372502/1‑100 (MQ=255)
        cAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgcg                                                                                    <  1:3944950/100‑1 (MQ=255)
        cAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgcg                                                                                    >  1:3840379/1‑100 (MQ=255)
         aGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgcgg                                                                                   >  1:1263314/1‑100 (MQ=255)
          gATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgcggn                                                                                  <  1:2472866/100‑2 (MQ=255)
          gATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTgcggc                                                                                  <  2:171667/100‑1 (MQ=255)
            tAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGa                                                                                >  2:1601413/1‑100 (MQ=255)
             aaTCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAATATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGAc                                                                               <  1:5395366/100‑1 (MQ=255)
             aaTCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGAc                                                                               <  1:5562846/100‑1 (MQ=255)
             aaTCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGAc                                                                               <  2:4061732/100‑1 (MQ=255)
             aaTCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGAc                                                                               <  2:4015223/100‑1 (MQ=255)
              aTCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACg                                                                              <  1:263691/100‑1 (MQ=255)
                ccaccaGTTGCTGCACGCTTTGTGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTc                                                                            <  2:4228908/100‑1 (MQ=255)
                ccaccaGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTc                                                                            <  2:3144159/100‑1 (MQ=255)
                ccaccaGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCCCGGTGCGGCGACGTc                                                                            >  2:1283267/1‑100 (MQ=255)
                 caccaGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCa                                                                           <  1:1884625/100‑1 (MQ=255)
                  accaGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAg                                                                          >  1:4320374/1‑100 (MQ=255)
                   ccaGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGc                                                                         <  1:5006145/100‑1 (MQ=255)
                    caGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCa                                                                        <  2:3086514/100‑1 (MQ=255)
                     aGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCAt                                                                       >  1:908004/1‑100 (MQ=255)
                       ttGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCt                                                                     <  2:599771/100‑1 (MQ=255)
                       ttGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCt                                                                     <  2:3035760/100‑1 (MQ=255)
                        tgctgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTg                                                                    >  1:3445591/1‑100 (MQ=255)
                         gctgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCGTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTgg                                                                   >  2:4129928/1‑100 (MQ=255)
                          ctgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGc                                                                  >  2:4731658/1‑100 (MQ=255)
                          ctgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCGCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGc                                                                  >  2:3312510/1‑100 (MQ=255)
                          ctgcACGCTTTGCGGGTCGTGGCTGTATAGCTCGAACATCGGCTCTTCGAAACTCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGc                                                                  <  1:1105536/100‑1 (MQ=255)
                           tgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCa                                                                 <  1:63542/100‑1 (MQ=255)
                           tgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCa                                                                 <  1:2186838/100‑1 (MQ=255)
                           tgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCa                                                                 >  1:2746795/1‑100 (MQ=255)
                           tgcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCa                                                                 <  2:4651345/100‑1 (MQ=255)
                            gcACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCAt                                                                >  1:5404325/1‑100 (MQ=255)
                             cACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATg                                                               <  1:3839643/100‑1 (MQ=255)
                             cACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATg                                                               >  1:4761660/1‑100 (MQ=255)
                                   ttGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGtt                                                         <  2:2720490/100‑1 (MQ=255)
                                     gCGGGTCGTGGGTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGGCGTCAGCATCTGGCATGTCCGtttt                                                       <  2:4387070/100‑1 (MQ=255)
                                       gggTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTAc                                                     <  1:3271983/100‑1 (MQ=255)
                                       gggTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTAc                                                     >  1:4900456/1‑100 (MQ=255)
                                       gggTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTAc                                                     <  2:3669444/100‑1 (MQ=255)
                                         gTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtg                                                   >  2:4619189/1‑100 (MQ=255)
                                           cGTGGCTGTTTCGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgat                                                 <  2:2420251/100‑1 (MQ=255)
                                           cGTGGCTGTTTAGCTCGAACATGGGCTCTTCGAAACCCGTCTCAAGAAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgat                                                 >  2:4735370/1‑100 (MQ=255)
                                           cGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgat                                                 >  2:1099124/1‑100 (MQ=255)
                                            gTGGCTGTTTAGCTCGAACATCGGCTCTTTGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatg                                                <  1:1717048/100‑1 (MQ=255)
                                            gTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatn                                                <  2:2037589/100‑2 (MQ=255)
                                            gTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatg                                                <  1:2971304/100‑1 (MQ=255)
                                            gTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatg                                                <  1:3248721/100‑1 (MQ=255)
                                             tGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatga                                               <  2:1012497/100‑1 (MQ=255)
                                             tGGCTGTTTAACTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACtgatga                                               >  2:5063403/1‑100 (MQ=255)
                                              ggCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGAc                                              >  2:617491/1‑100 (MQ=255)
                                                cTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACgg                                            <  1:3777632/100‑1 (MQ=255)
                                                 tGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGc                                           >  1:3656476/1‑100 (MQ=255)
                                                 tGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATATGGCATGTCCGTTTTACTGATGACGGc                                           <  1:596249/100‑1 (MQ=255)
                                                  gTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCg                                          >  2:2556927/1‑100 (MQ=255)
                                                     tAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGAtt                                       <  1:777097/100‑1 (MQ=255)
                                                     tAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGAtt                                       <  2:138767/100‑1 (MQ=255)
                                                     tAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGAtt                                       >  1:5049587/1‑100 (MQ=255)
                                                      aGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGAttt                                      <  2:1523628/100‑1 (MQ=255)
                                                       gCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTg                                     <  2:5433031/100‑1 (MQ=255)
                                                       gCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTg                                     >  1:5452677/1‑100 (MQ=255)
                                                       gCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTg                                     >  1:3606077/1‑100 (MQ=255)
                                                         tCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGcc                                   <  2:3413537/100‑1 (MQ=255)
                                                         tCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAACAACGTTCGCGTTGATGATACGTCAGCATCTGGCATGTGCGATTTACTGATGAGGGCGATTTGcc                                   >  1:5414568/1‑100 (MQ=255)
                                                          cGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCg                                  <  1:1980101/100‑1 (MQ=255)
                                                          cGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCg                                  <  1:3114997/100‑1 (MQ=255)
                                                          cGAACATCGGCTCTTCGAAACCCGTCTCAAGAAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCg                                  >  1:4461236/1‑100 (MQ=255)
                                                           gAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGt                                 >  2:2198578/1‑100 (MQ=255)
                                                             aCATCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGACATGTCCGTTTTACTGATGACGGCGATTTGCCGttt                               <  1:227636/100‑1 (MQ=255)
                                                               gtCGGCTCTTCGAATCCCGTCTCAAGCGACAACGTTCGCGTTGCGGCGACGTCAGCATTTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGc                             <  2:5227194/99‑1 (MQ=255)
                                                                tCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCt                            <  2:697762/100‑1 (MQ=255)
                                                                tCGGCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCt                            >  2:2443703/1‑100 (MQ=255)
                                                                  ggCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGa                          >  1:3129793/1‑100 (MQ=255)
                                                                   gccctTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGCCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGACTTGCCGTTTGCTGAc                         >  2:4097183/4‑100 (MQ=255)
                                                                   gCTCTTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGAc                         <  1:2569454/100‑1 (MQ=255)
                                                                    ctctTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACg                        <  2:4493265/100‑1 (MQ=255)
                                                                     tctTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGc                       <  2:2151031/100‑1 (MQ=255)
                                                                      ctTCGAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGcc                      >  1:2004406/1‑100 (MQ=255)
                                                                          gAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAata                  <  1:4622404/100‑1 (MQ=255)
                                                                          gAAACCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAata                  >  2:4844969/1‑100 (MQ=255)
                                                                          gAAACCCGTCTCAAGCAATAACGTGCGCGGTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATATGCCGTTTGCTGACGCCAata                  >  1:5193942/1‑100 (MQ=255)
                                                                            aaCCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATc                >  2:3980889/1‑100 (MQ=255)
                                                                             aCCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATcc               <  1:4724489/100‑1 (MQ=255)
                                                                             aCCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATcc               <  2:528011/100‑1 (MQ=255)
                                                                             aCCCGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATcc               <  1:4725661/100‑1 (MQ=255)
                                                                              cccGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCa              >  1:4306156/1‑100 (MQ=255)
                                                                               ccGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAg             <  1:4799083/100‑1 (MQ=255)
                                                                                cGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGc            <  1:2368193/100‑1 (MQ=255)
                                                                                cGTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGc            <  2:3740274/100‑1 (MQ=255)
                                                                                 gTCTCAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCa           >  2:3179893/1‑100 (MQ=255)
                                                                                    tcAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAAcc        <  2:3507559/100‑1 (MQ=255)
                                                                                     cAAGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAAccc       >  2:435196/1‑100 (MQ=255)
                                                                                      aaGCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCg      >  1:3877642/1‑100 (MQ=255)
                                                                                        gCAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCggc    >  2:5016274/1‑100 (MQ=255)
                                                                                         cAATAACGTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCggca   >  2:3113998/1‑100 (MQ=255)
                                                                                          aaTAACGTCCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCggcag  <  2:1954974/100‑1 (MQ=255)
                                                                                                |                                                                                             
AGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCGGCAG  >  NC_000913/2575024‑2575213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: