BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,422	A→C	V223V (GTA→GTC)	ung →	uracil‑DNA‑glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,422	0	A	C	98.0%	858.9 / 1.0	244	V223V (GTA→GTC)	ung	uracil‑DNA‑glycosylase
Reads supporting (aligned to +/- strand): ref base A (2/3); new base C (116/123); total (118/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCA  >  NC_000913/2717326‑2717518

ttCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTa                                                                                               >  1:1920412/1‑100 (MQ=255)

  cGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTAcc                                                                                             <  1:1703026/100‑1 (MQ=255)

   ggCGCATCGTTGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCg                                                                                            >  2:403234/1‑100 (MQ=255)

   ggCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCg                                                                                            <  2:1367354/100‑1 (MQ=255)

   ggCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCg                                                                                            <  2:2734332/100‑1 (MQ=255)

     cgcATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGc                                                                                          <  2:2250383/100‑1 (MQ=255)

        aTCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCaga                                                                                       >  2:318872/1‑100 (MQ=255)

        aTCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCaga                                                                                       <  1:339394/100‑1 (MQ=255)

        aTCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCaga                                                                                       >  2:4189091/1‑100 (MQ=255)

          cGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCagaga                                                                                     >  2:4833152/1‑100 (MQ=255)

          cGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCagaga                                                                                     >  2:3962555/1‑100 (MQ=255)

           gTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCagagag                                                                                    <  2:1614557/100‑1 (MQ=255)

           gTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCagagag                                                                                    >  1:2892239/1‑100 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  <  2:2637656/100‑1 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGGGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAgagtg                                                                                  <  2:1395889/100‑1 (MQ=255)

               aTTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgag                                                                                <  2:2305998/100‑1 (MQ=255)

               aTTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgag                                                                                <  1:2065948/100‑1 (MQ=255)

               aTTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgag                                                                                <  2:357252/100‑1 (MQ=255)

                   tttGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTaaa                                                                            >  2:2927085/1‑100 (MQ=255)

                     tGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAAtt                                                                          <  1:1485618/100‑1 (MQ=255)

                      ggCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAAttt                                                                         >  1:1116540/1‑100 (MQ=255)

                         tGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      <  1:1352507/100‑1 (MQ=255)

                          gCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgg                                                                     <  2:2583725/100‑1 (MQ=255)

                          gCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgg                                                                     <  2:2359908/100‑1 (MQ=255)

                           cAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCggg                                                                    <  1:2490960/100‑1 (MQ=255)

                           cAACCATTTTGTGCTGGCAAATAAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCggg                                                                    <  1:666664/100‑1 (MQ=255)

                            aaCCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgggg                                                                   <  2:113392/100‑1 (MQ=255)

                            aaCCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgggg                                                                   >  1:1730300/1‑100 (MQ=255)

                            aaCCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGAGTGTCAATTTGCgggg                                                                   <  1:4885295/100‑1 (MQ=255)

                             aCCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGa                                                                  <  1:3768038/100‑1 (MQ=255)

                               taTTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGaaa                                                                <  1:4362373/99‑1 (MQ=255)

                                aTTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGAAGCCGAATGACTGGATGCCAGTCTTACCGGCAGAGAGCGAGTATAGTAGCGCGCAAAt                                                               >  2:518206/1‑100 (MQ=255)

                                    tgtgCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCg                                                           <  2:4168989/100‑1 (MQ=255)

                                    tgtgCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCg                                                           <  1:2764908/100‑1 (MQ=255)

                                     gtgCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCgg                                                          <  1:4822169/100‑1 (MQ=255)

                                      tgCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGa                                                         >  1:4706759/1‑100 (MQ=255)

                                       gCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGAt                                                        <  1:1505998/100‑1 (MQ=255)

                                       gCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGAt                                                        >  1:743936/1‑100 (MQ=255)

                                        cTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATg                                                       >  2:1709034/1‑100 (MQ=255)

                                        cTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATg                                                       >  2:3512959/1‑100 (MQ=255)

                                         tGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGAGGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATgg                                                      <  2:842682/100‑1 (MQ=255)

                                          ggCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGc                                                     <  1:4280723/100‑1 (MQ=255)

                                          ggCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGc                                                     <  1:4397627/100‑1 (MQ=255)

                                           gCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCa                                                    <  2:3669865/100‑1 (MQ=255)

                                            cAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCag                                                   <  1:321881/100‑1 (MQ=255)

                                            cAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCag                                                   >  1:4564265/1‑100 (MQ=255)

                                            cAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGGCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCag                                                   >  2:4979163/1‑100 (MQ=255)

                                             aaaTCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCaga                                                  >  2:4894932/1‑100 (MQ=255)

                                               aTCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGt                                                >  2:2445631/1‑100 (MQ=255)

                                                 cAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTg                                              <  2:5396923/100‑1 (MQ=255)

                                                  aGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGc                                             >  1:3051147/1‑100 (MQ=255)

                                                  aGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGc                                             >  1:3452658/1‑100 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  1:3151122/1‑100 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  1:421033/1‑100 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  2:1544783/1‑100 (MQ=255)

                                                      gCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAcc                                         >  2:3042612/1‑100 (MQ=255)

                                                       cTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGCGAGGGAGTAAATTTGCGGGGAAGTGCGGGATGGAAGAGTTGACAccg                                        >  2:5121490/1‑99 (MQ=255)

                                                        tGGAACAATGTGGCGTGACGCTTATTGGCTGGGGGCCAGTCTTAGCGGTAGAGAGTGAGTAAATTTGCGGGGGAATGCCGGATGGCAGAGTTGGCTCCCg                                       <  2:1888084/100‑1 (MQ=255)

                                                          gAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGc                                     >  2:215772/1‑100 (MQ=255)

                                                          gAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGGGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGc                                     >  1:5078569/1‑100 (MQ=255)

                                                           aacaacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCt                                    >  2:5306697/1‑100 (MQ=255)

                                                             caacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGa                                  <  1:4964353/100‑1 (MQ=255)

                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAt                                 >  2:3692747/1‑100 (MQ=255)

                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAt                                 >  1:5019331/1‑100 (MQ=255)

                                                               acGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAACGGCCGGATGGCAGAGTTGTCACCCGGCTGAtt                                >  2:4299689/1‑100 (MQ=255)

                                                               acGTGGCGAGACGCCGATTGACTGGATGCCAGCCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAtt                                <  1:5102926/100‑1 (MQ=255)

                                                                cGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAttt                               >  2:445929/1‑100 (MQ=255)

                                                                cGTGGCGAGACGCCGATTGACTGGACGCCAGTCTTACCGGCAGAGAGTGTGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAttt                               <  2:1793463/100‑1 (MQ=255)

                                                                 gTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTa                              <  1:29756/100‑1 (MQ=255)

                                                                 gTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTa                              <  1:1262778/100‑1 (MQ=255)

                                                                 gTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTa                              >  2:1009183/1‑100 (MQ=255)

                                                                  tGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTAt                             <  2:4119401/100‑1 (MQ=255)

                                                                    gCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCa                           <  2:3191575/100‑1 (MQ=255)

                                                                     cGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAg                          <  2:3364396/100‑1 (MQ=255)

                                                                       agaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGc                        <  1:2150887/100‑1 (MQ=255)

                                                                        gaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCt                       >  1:5560923/1‑100 (MQ=255)

                                                                        gaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCt                       >  2:2009502/1‑100 (MQ=255)

                                                                          cGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCttt                     >  2:5029718/1‑100 (MQ=255)

                                                                           gCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTa                    <  2:1662024/100‑1 (MQ=255)

                                                                            ccGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTAt                   <  1:5391174/100‑1 (MQ=255)

                                                                             cGATTGACTGGATGCCAGTCTTACCGGCAGAGCGTGAGTAAATTTGCGGAGAAATGCCGGATGGCAGAGTTGCAACCCGGCTGATTTCTCAGGATTTAtt                  >  2:529035/1‑100 (MQ=255)

                                                                             cGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTAtt                  >  1:3290488/1‑100 (MQ=255)

                                                                             cGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTAtt                  <  1:3535325/100‑1 (MQ=255)

                                                                               aTTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCt                <  1:1038925/100‑1 (MQ=255)

                                                                                  gACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGAc             >  1:2452192/1‑100 (MQ=255)

                                                                                  gACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGAc             <  1:407188/100‑1 (MQ=255)

                                                                                    cTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGc           >  1:3033457/1‑100 (MQ=255)

                                                                                    cTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGc           >  1:3559162/1‑100 (MQ=255)

                                                                                     tGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGcc          <  1:2710285/100‑1 (MQ=255)

                                                                                      ggATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGcca         >  2:2727699/1‑100 (MQ=255)

                                                                                      ggATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGcca         >  1:927830/1‑100 (MQ=255)

                                                                                      ggATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGcca         >  1:4931523/1‑100 (MQ=255)

                                                                                        aTGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCTGCTGATTTATCAGGCTTTATTCTGACGccacc       <  2:4948612/100‑1 (MQ=255)

                                                                                        aTGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacc       >  1:3220202/1‑100 (MQ=255)

                                                                                        aTGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacc       >  1:1132495/1‑100 (MQ=255)

                                                                                         tGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacca      >  1:3976302/1‑100 (MQ=255)

                                                                                          gCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCAt     >  1:2948693/1‑100 (MQ=255)

                                                                                          gCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCAt     >  2:3488260/1‑100 (MQ=255)

                                                                                            cAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTc   >  1:3154080/1‑100 (MQ=255)

                                                                                             aGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCa  <  2:3425658/100‑1 (MQ=255)

TTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCA  >  NC_000913/2717326‑2717518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`