Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,869,491 A→C L10R (CTG→CGG)  pcm ← L‑isoaspartate protein carboxylmethyltransferase type II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,869,4910AC99.1% 768.7 / NA 214L10R (CTG→CGG) pcmL‑isoaspartate protein carboxylmethyltransferase type II
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (120/91);  total (123/91)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TTGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAATC  >  NC_000913/2869394‑2869587
                                                                                                 |                                                                                                
ttGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGa                                                                                                <  2:662382/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              >  1:1372341/1‑100 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  2:489276/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              >  2:2690630/1‑100 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              >  1:3570176/1‑100 (MQ=255)
   ttCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGt                                                                                             <  1:803901/100‑1 (MQ=255)
     cAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGc                                                                                           >  1:4073771/1‑100 (MQ=255)
       aaCGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCtt                                                                                         <  2:3404332/100‑1 (MQ=255)
         cGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGt                                                                                       <  1:2027329/100‑1 (MQ=255)
         cGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGt                                                                                       <  2:3850606/100‑1 (MQ=255)
            ttCATCAACGAATTTTTCAGGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTAcg                                                                                    >  2:5021983/1‑100 (MQ=255)
             tcatcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAcgc                                                                                   >  1:5270457/1‑100 (MQ=255)
              catcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAcgcg                                                                                  >  2:1457583/1‑100 (MQ=255)
              catcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAcgcg                                                                                  >  1:4877636/1‑100 (MQ=255)
              catcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAcgcg                                                                                  >  1:4810813/1‑100 (MQ=255)
               atcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGt                                                                                 >  2:1510808/1‑100 (MQ=255)
               atcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGt                                                                                 >  2:4052188/1‑100 (MQ=255)
                tcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTc                                                                                >  2:3835260/1‑100 (MQ=255)
                tcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTc                                                                                <  2:3685301/100‑1 (MQ=255)
                tcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTc                                                                                <  2:4743167/100‑1 (MQ=255)
                 caACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCt                                                                               <  1:5126475/100‑1 (MQ=255)
                 caACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCt                                                                               >  2:4576758/1‑100 (MQ=255)
                  aaCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTg                                                                              >  2:1935105/1‑100 (MQ=255)
                  aaCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTg                                                                              >  2:3557213/1‑100 (MQ=255)
                   aCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGc                                                                             >  2:1177314/1‑100 (MQ=255)
                   aCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGc                                                                             <  1:2552984/100‑1 (MQ=255)
                    cGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCt                                                                            >  2:5290651/1‑100 (MQ=255)
                    cGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCt                                                                            <  1:5055893/100‑1 (MQ=255)
                      aaTTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCtga                                                                          >  2:5466054/1‑98 (MQ=255)
                      aaTTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTa                                                                          >  1:3904758/1‑100 (MQ=255)
                       aTTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAc                                                                         >  1:3331668/1‑100 (MQ=255)
                        tttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAcc                                                                        <  1:157842/100‑1 (MQ=255)
                        tttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAcc                                                                        <  2:5351258/100‑1 (MQ=255)
                         ttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTTAATACCTTGCGCACGTAATTGATCCCGAAGAGCTTGTACGCGTCTGCTTACCa                                                                       <  1:4659932/100‑1 (MQ=255)
                          tttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCAt                                                                      <  2:3323563/100‑1 (MQ=255)
                          tttAACGCGGCACTGCGGCAAGCGCATTCAGCACCTGCTTATCCTGAATCCCTTACGCACGTAATTCATCCATAAGTGCTTGTACGCGTCTGCTTCCCAt                                                                      >  2:743304/1‑100 (MQ=255)
                             cACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGc                                                                   <  2:4688477/100‑1 (MQ=255)
                               cgcgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGt                                                                 >  1:3337417/1‑100 (MQ=255)
                               cgcgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGt                                                                 <  1:924658/100‑1 (MQ=255)
                                 cgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGc                                                               >  2:4942766/1‑100 (MQ=255)
                                   gCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCa                                                             >  2:2404661/1‑100 (MQ=255)
                                     aCGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAAc                                                           <  1:2441513/100‑1 (MQ=255)
                                      cggcggCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACt                                                          <  2:1094038/100‑1 (MQ=255)
                                         cggcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTccc                                                       <  2:1738725/100‑1 (MQ=255)
                                         cggcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTccc                                                       >  1:309499/1‑100 (MQ=255)
                                          ggcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCa                                                      <  1:2728843/100‑1 (MQ=255)
                                           gcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCAc                                                     <  2:4520204/100‑1 (MQ=255)
                                           gcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCAc                                                     <  1:2097297/100‑1 (MQ=255)
                                            cAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACg                                                    >  1:1017194/1‑100 (MQ=255)
                                            cAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACg                                                    >  1:2470753/1‑100 (MQ=255)
                                                tGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGt                                                <  2:3159994/100‑1 (MQ=255)
                                                tGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGt                                                <  1:1784133/100‑1 (MQ=255)
                                                tGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGt                                                >  1:992324/1‑100 (MQ=255)
                                                  cATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGttt                                              >  1:3822264/1‑100 (MQ=255)
                                                   aTTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTa                                             <  1:867531/100‑1 (MQ=255)
                                                     tCAGCACCTGCTCATCGTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAAc                                           <  1:4589855/100‑1 (MQ=255)
                                                     tCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAAc                                           <  1:4325597/100‑1 (MQ=255)
                                                      cAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAAcc                                          <  2:2155602/100‑1 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        <  2:4703874/100‑1 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        <  2:3290679/100‑1 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        >  1:499270/1‑100 (MQ=255)
                                                          aCCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTc                                      <  2:2226818/100‑1 (MQ=255)
                                                          aCCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTc                                      <  1:77299/100‑1 (MQ=255)
                                                           ccTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCt                                     >  1:4122651/1‑100 (MQ=255)
                                                             tGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGTGTGCCAACTCCCACGCTGTTTAACCAGTCTGa                                   >  2:1813486/1‑100 (MQ=255)
                                                             tGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGa                                   >  1:5351741/1‑100 (MQ=255)
                                                              gCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGaa                                  >  1:3398795/1‑100 (MQ=255)
                                                              gCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGaa                                  >  1:4243487/1‑100 (MQ=255)
                                                               cTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGaaa                                 >  1:2812426/1‑100 (MQ=255)
                                                                tCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAAc                                <  2:1319269/100‑1 (MQ=255)
                                                                 cATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTACCAAGCTGTTTAACCAGTCTGACAcc                               >  2:3467747/1‑100 (MQ=255)
                                                                  aTCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACca                              >  1:3239188/1‑100 (MQ=255)
                                                                   tCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcac                             >  2:2960920/1‑100 (MQ=255)
                                                                   tCCTGAATACCGTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcac                             <  2:2508494/100‑1 (MQ=255)
                                                                    ccTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcaca                            <  2:2555282/100‑1 (MQ=255)
                                                                    ccTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcaca                            <  1:2660894/100‑1 (MQ=255)
                                                                    ccTGAATACCTAGCGCACGTAATTCATCCTGAAGTGCTTGTACGCGTCTGCTTAGCATTGAGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcaca                            >  1:5201067/1‑100 (MQ=255)
                                                                      tGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATc                          >  2:1522323/1‑100 (MQ=255)
                                                                      tGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATc                          >  1:949558/1‑100 (MQ=255)
                                                                      tGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATc                          <  1:5492757/100‑1 (MQ=255)
                                                                       gAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCt                         >  2:523076/1‑100 (MQ=255)
                                                                       gAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCt                         <  1:4630185/100‑1 (MQ=255)
                                                                         aTACGTTGCGAACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTg                       <  2:2771967/100‑1 (MQ=255)
                                                                             cTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgcg                   <  2:3930255/100‑1 (MQ=255)
                                                                             cTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgcg                   <  1:4391717/100‑1 (MQ=255)
                                                                                 cgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTAt               <  2:306593/100‑1 (MQ=255)
                                                                                  gcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATg              >  2:1754592/1‑100 (MQ=255)
                                                                                   cACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATgc             >  2:437884/1‑100 (MQ=255)
                                                                                       tAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAg         >  2:868151/1‑100 (MQ=255)
                                                                                       tAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAg         <  1:1791050/100‑1 (MQ=255)
                                                                                       tAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAg         >  2:3097854/1‑100 (MQ=255)
                                                                                       tAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAg         >  2:1705208/1‑100 (MQ=255)
                                                                                        aaTTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGt        >  2:4438813/1‑100 (MQ=255)
                                                                                         cttGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGtt       <  2:4185104/99‑1 (MQ=255)
                                                                                         aTTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGtt       >  2:4352849/1‑100 (MQ=255)
                                                                                          ttGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACACCTTGCGCGCTATGCGCAGTTa      >  1:313769/1‑100 (MQ=255)
                                                                                           tGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTaa     >  1:2177665/1‑100 (MQ=255)
                                                                                           tGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGAGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTaa     >  1:2837494/1‑100 (MQ=255)
                                                                                             aTCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAAt   <  2:4791338/100‑1 (MQ=255)
                                                                                              tCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAATc  >  2:2123618/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAATC  >  NC_000913/2869394‑2869587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑