Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,906,363 T→G L218* (TTA→TGA)  ygcG → TPM domain protein, putative phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,906,3630TG98.6% 978.4 / 1.8 277L218* (TTA→TGA) ygcGTPM domain protein, putative phosphatase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base G (136/137);  total (137/140)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 284 total aligned reads displayed.

CAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCA  >  NC_000913/2906264‑2906459
                                                                                                   |                                                                                                
  gTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGAt                                                                                                >  1:4782045/1‑100 (MQ=255)
  gTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGAATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGAt                                                                                                <  2:1435440/100‑1 (MQ=255)
    gATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTc                                                                                              >  2:2229932/1‑100 (MQ=255)
     aTGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTcc                                                                                             >  1:1029430/1‑100 (MQ=255)
      tGTTCTTCCCTTTCTGGTTTTTTTTTTTTTTTTTTAATTTTAGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTCGATTGATTCCt                                                                                            >  2:3271240/1‑100 (MQ=255)
      tGTTCTTCCCTTTCTGCTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCt                                                                                            <  1:4584453/100‑1 (MQ=255)
       gTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTg                                                                                           >  1:5264127/1‑100 (MQ=255)
        ttcttcCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGt                                                                                          >  2:3621035/1‑100 (MQ=255)
        ttcttcCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGt                                                                                          <  1:2769980/100‑1 (MQ=255)
         tcttcCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGtt                                                                                         >  1:3465641/1‑100 (MQ=255)
         tcttcCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGtt                                                                                         >  1:4489929/1‑100 (MQ=255)
          cttcCCTTTCTGGTTTTTTTTTTTTTTTTGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTTGATTGATTCCTGTtc                                                                                        >  2:441275/1‑100 (MQ=255)
          cttcCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTtc                                                                                        <  1:875359/100‑1 (MQ=255)
             cccTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTcc                                                                                     <  2:2165089/100‑1 (MQ=255)
             cccTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTcc                                                                                     >  2:3426640/1‑100 (MQ=255)
             cccTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTcc                                                                                     <  1:2818927/100‑1 (MQ=255)
             cccTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTcc                                                                                     >  2:5145999/1‑100 (MQ=255)
               cTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGa                                                                                   >  1:2744427/1‑100 (MQ=255)
                tttCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGAt                                                                                  >  2:1174549/1‑100 (MQ=255)
                   cTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATaaa                                                                               >  1:1056496/1‑100 (MQ=255)
                     ggTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAt                                                                             >  2:38846/1‑100 (MQ=255)
                      gTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAtt                                                                            <  1:3122495/100‑1 (MQ=255)
                        tttttCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAtttt                                                                          <  1:668884/100‑1 (MQ=255)
                        tttttCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAtttt                                                                          <  2:2142154/100‑1 (MQ=255)
                         ttttCATCAAGGCAGTAATTTTTGTCGCGCACGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAttttt                                                                         <  2:4705211/100‑1 (MQ=255)
                         ttttCATAAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAttttt                                                                         <  2:5197639/100‑1 (MQ=255)
                          tttCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAAtttttt                                                                        <  2:3556919/100‑1 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     <  1:39219/100‑1 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     >  2:2968571/1‑100 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     >  2:1681359/1‑100 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     >  2:1533452/1‑100 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     <  1:5095838/100‑1 (MQ=255)
                             catcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGCGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCa                                                                     >  2:3102639/1‑100 (MQ=255)
                              atcaAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCAt                                                                    <  1:1910288/100‑1 (MQ=255)
                                caAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTg                                                                  >  2:2809124/1‑100 (MQ=255)
                                caAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTg                                                                  >  2:15811/1‑100 (MQ=255)
                                caAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTg                                                                  >  2:3531340/1‑100 (MQ=255)
                                   ggCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTg                                                               <  1:1812612/100‑1 (MQ=255)
                                     cAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTa                                                             <  1:3294571/100‑1 (MQ=255)
                                     cAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTa                                                             >  1:3545677/1‑100 (MQ=255)
                                      aGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGttt                                                            >  2:648033/1‑98 (MQ=255)
                                      aGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTAt                                                            >  1:4095166/1‑100 (MQ=255)
                                           tttttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTcc                                                       <  2:4626947/100‑1 (MQ=255)
                                           tttttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTcc                                                       <  1:5349955/100‑1 (MQ=255)
                                            ttttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTGTTTTCct                                                      <  1:4370724/100‑1 (MQ=255)
                                            ttttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCct                                                      <  1:4692317/100‑1 (MQ=255)
                                             tttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCctt                                                     >  1:1404578/1‑100 (MQ=255)
                                              ttGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCcttc                                                    <  2:4954919/100‑1 (MQ=255)
                                                gTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCcttctt                                                  >  1:2025667/1‑100 (MQ=255)
                                                  cgcgCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCtttt                                                <  2:5413505/100‑1 (MQ=255)
                                                   gcgcATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCttttt                                               >  1:1603089/1‑100 (MQ=255)
                                                    cgcATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCtttttt                                              <  2:2659660/100‑1 (MQ=255)
                                                      cATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTAc                                            <  2:374834/100‑1 (MQ=255)
                                                      cATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTAc                                            >  2:3920210/1‑100 (MQ=255)
                                                      cATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTAc                                            <  1:3341969/100‑1 (MQ=255)
                                                      cATGTAAAAGTGGAGTCTGTATTTCAGCAATCCATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTAc                                            <  2:5497530/100‑1 (MQ=255)
                                                       aTGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACt                                           <  2:2790844/100‑1 (MQ=255)
                                                         gTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACttt                                         >  2:5340135/1‑100 (MQ=255)
                                                          tAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTc                                        >  1:2965824/1‑100 (MQ=255)
                                                           aaaaGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCa                                       <  1:4478346/100‑1 (MQ=255)
                                                           aaaaGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCa                                       >  1:1953823/1‑100 (MQ=255)
                                                           aaaaGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCa                                       <  2:2267087/100‑1 (MQ=255)
                                                             aaGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCAcc                                     <  2:3832312/100‑1 (MQ=255)
                                                             aaGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCAcc                                     <  1:784603/100‑1 (MQ=255)
                                                              aGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCCGTCTTTTCCTTCTTTTTTACTTTCACCa                                    >  1:2432364/1‑100 (MQ=255)
                                                               gTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCat                                   <  2:5026660/100‑1 (MQ=255)
                                                                tGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTTTTTTTTTTTATTTTCCTTCTTTTTTACTTTCACCata                                  >  2:1442074/1‑100 (MQ=255)
                                                                 ggAGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCatat                                 <  2:4475781/100‑1 (MQ=255)
                                                                   aGTCTGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATAttt                               >  2:3241892/1‑100 (MQ=255)
                                                                       tGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCTCCATATTTATgg                           >  2:2053642/1‑100 (MQ=255)
                                                                       tGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATgg                           >  2:3664263/1‑100 (MQ=255)
                                                                       tGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATgg                           <  2:949604/100‑1 (MQ=255)
                                                                       tGTATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATgg                           <  2:2542941/100‑1 (MQ=255)
                                                                         tATTTCAGCGATCTATCTTTTAGCTTGATTCCTGTTCTCCGATAAAATTTTTTCTATTGCAGTATTTTCCTTCTTTTTTACTTTGACCATATTTATGGTc                         <  1:4851590/100‑1 (MQ=255)
                                                                         tATTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATGTTTTCCATGGCTGTATTTTCCTTCTTTTTTACTTTCACCAtatgtatgtgc                         >  2:1362837/1‑97 (MQ=255)
                                                                          aTTTCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCt                        <  1:5157970/100‑1 (MQ=255)
                                                                            ttCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCttt                      >  1:2300138/1‑100 (MQ=255)
                                                                             tCAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTCTCCATTGCTGTATTTTCCATCTTTTTTACTTTCACCATATTTATGGTCTTTAc                    <  2:5083071/100‑1 (MQ=255)
                                                                             tCAGCAATCTATCGTTTAGATTGATTACTGTCCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTa                     <  2:4307031/100‑1 (MQ=255)
                                                                              cAGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTAc                    >  1:3741217/1‑100 (MQ=255)
                                                                               aGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTAcc                   <  1:3209210/100‑1 (MQ=255)
                                                                               aGCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTAcc                   <  1:2627538/100‑1 (MQ=255)
                                                                                gCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTCTTTACTTTCACCATATTGATGGTCTTTACCt                  >  2:4315548/1‑100 (MQ=255)
                                                                                gCAATCTATCTTTTAGATTGATTCCTGTTCTCCGATAAAATGTTTTCCATTGCTGTATGTTCCTTCGTTGTTTCTTTCACCTGATTTTTGGTGTTGACCt                  >  2:249254/1‑100 (MQ=255)
                                                                                   atctatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGtt               >  1:2176817/1‑100 (MQ=255)
                                                                                    tctatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGttt              <  2:434726/100‑1 (MQ=255)
                                                                                    tctatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGttt              <  2:4204107/100‑1 (MQ=255)
                                                                                    tctatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGttt              >  1:1196719/1‑100 (MQ=255)
                                                                                     ctatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTa             >  1:2594633/1‑100 (MQ=255)
                                                                                      tatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTAt            >  2:491391/1‑100 (MQ=255)
                                                                                      tatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTAt            <  1:3860204/100‑1 (MQ=255)
                                                                                      tatctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTAt            <  1:4008002/100‑1 (MQ=255)
                                                                                       atctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTAtg           <  2:3710376/100‑1 (MQ=255)
                                                                                         ctTTTAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCGTTCTTTTTTACTTTCACCATCTTCATGGTCTTTAGCTGTTTAtgtg         >  2:4567144/1‑100 (MQ=255)
                                                                                           tttAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTa       >  2:2742168/1‑100 (MQ=255)
                                                                                           tttAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTa       >  1:552125/1‑100 (MQ=255)
                                                                                            ttAGATTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTAc      <  2:1023812/100‑1 (MQ=255)
                                                                                            ttAGATTGATTCCTGTTCTCCGATAAAATTTTATCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTAc      <  1:5110129/100‑1 (MQ=255)
                                                                                               gattgattCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTc   >  1:659952/1‑100 (MQ=255)
                                                                                                aTTGATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCa  >  1:3048391/1‑100 (MQ=255)
                                                                                                   |                                                                                                
CAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCA  >  NC_000913/2906264‑2906459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: