BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,934,721	T→G	S163A (TCG→GCG)	fucP →	L‑fucose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,934,721	0	T	G	98.0%	694.1 / ‑0.4	197	S163A (TCG→GCG)	fucP	L‑fucose transporter
Reads supporting (aligned to +/- strand): ref base T (3/1); new base G (100/93); total (103/94)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TAGGTTGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACTCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGTCTCCAG  >  NC_000913/2934626‑2934817

  ggTTGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTg                                                                                            >  1:3512754/1‑100 (MQ=255)

   gTTGTCTGGAAACCGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTgg                                                                                           >  2:3598240/1‑100 (MQ=255)

    ttGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGgc                                                                                          >  2:4117206/1‑100 (MQ=255)

    gtGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGgc                                                                                          <  1:4526456/99‑1 (MQ=255)

     tGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGgcg                                                                                         >  2:292573/1‑100 (MQ=255)

     tGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGgcg                                                                                         >  2:2010471/1‑100 (MQ=255)

       tCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCa                                                                                       >  1:5039183/1‑100 (MQ=255)

        cTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCaa                                                                                      <  1:5059687/100‑1 (MQ=255)

        cTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCaa                                                                                      >  2:1620686/1‑100 (MQ=255)

        cTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCaa                                                                                      >  2:1821168/1‑100 (MQ=255)

           gAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCGCTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTa                                                                                   <  2:4266239/100‑1 (MQ=255)

             aaCTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATc                                                                                 <  1:112244/100‑1 (MQ=255)

              aCTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATcg                                                                                <  1:4182499/100‑1 (MQ=255)

               cTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATcgc                                                                               <  2:1349026/100‑1 (MQ=255)

               cTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATcgc                                                                               <  2:3958315/100‑1 (MQ=255)

               cTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATcgc                                                                               >  2:557942/1‑100 (MQ=255)

               cTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATcgc                                                                               <  2:2385970/100‑1 (MQ=255)

                  ccGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGt                                                                            >  1:5422380/1‑100 (MQ=255)

                  ccGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGt                                                                            <  1:4884177/100‑1 (MQ=255)

                   cGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGtt                                                                           >  2:861844/1‑100 (MQ=255)

                      aaaCCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTc                                                                        <  2:3896038/100‑1 (MQ=255)

                        aCCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCTCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCtt                                                                      <  2:5255779/100‑1 (MQ=255)

                         cccTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCttt                                                                     >  2:2651791/1‑100 (MQ=255)

                         cccTTTTGTTACGGTATTAGGGCCGAAAAGTAGTGGTCACTTCCGCTTAAATCTAGCGCAAACATTTAACGCGTTTGGCCCAATTATCGCGGTTGTCttt                                                                     >  2:510394/1‑100 (MQ=255)

                         cccTTTTGTTACGGTATTAGAGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCttt                                                                     >  1:4349338/1‑100 (MQ=255)

                          ccTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTg                                                                    >  2:4526392/1‑100 (MQ=255)

                           nttttGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTgg                                                                   >  1:4432360/2‑100 (MQ=255)

                           cTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTgg                                                                   >  2:2617536/1‑100 (MQ=255)

                           cTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTgg                                                                   >  1:17573/1‑100 (MQ=255)

                             tttGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGc                                                                 <  2:5382297/100‑1 (MQ=255)

                              ttGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCa                                                                <  1:3754381/100‑1 (MQ=255)

                              ttGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCa                                                                <  2:493161/100‑1 (MQ=255)

                                gTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCaaa                                                              >  1:5500210/1‑100 (MQ=255)

                                gTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCaaa                                                              >  1:2180511/1‑100 (MQ=255)

                                gTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGAGCAAACATTTAACGCGTTTGGCGCCATTATCGCGGTTGTCTTTGGGCaaa                                                              >  1:4717555/1‑100 (MQ=255)

                                 ttACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAg                                                             >  1:1996172/1‑100 (MQ=255)

                                  tACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGt                                                            >  1:5274087/1‑100 (MQ=255)

                                   aCGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTc                                                           >  2:1296264/1‑100 (MQ=255)

                                   aCGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTc                                                           <  1:2941420/100‑1 (MQ=255)

                                      gTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTa                                                        >  2:91743/1‑100 (MQ=255)

                                       tATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTAt                                                       <  1:5177070/100‑1 (MQ=255)

                                       tATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTAt                                                       <  1:3248549/100‑1 (MQ=255)

                                          tAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATAGCTGTTGTCTCTGGGCAAATTCTTCtttt                                                    >  2:4756492/1‑100 (MQ=255)

                                            gggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACTCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGt                                                  >  2:4584238/1‑100 (MQ=255)

                                            gggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGt                                                  >  1:3022458/1‑100 (MQ=255)

                                            gggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGt                                                  <  1:4249813/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  1:985379/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  2:2581189/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  1:2899363/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  1:815359/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  1:1474840/100‑1 (MQ=255)

                                             ggCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTc                                                 <  1:163780/100‑1 (MQ=255)

                                              gCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCt                                                <  1:850741/100‑1 (MQ=255)

                                                cGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTaa                                              <  1:1313634/100‑1 (MQ=255)

                                                cGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTaa                                              >  1:1519182/1‑100 (MQ=255)

                                                 ggAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAAc                                             >  1:4109438/1‑100 (MQ=255)

                                                  gAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACg                                            <  1:3902956/100‑1 (MQ=255)

                                                   aaaGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGt                                           >  1:2054419/1‑100 (MQ=255)

                                                      gtagtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGcc                                        <  1:3820977/100‑1 (MQ=255)

                                                      gtagtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGcc                                        <  2:3127245/100‑1 (MQ=255)

                                                      gtagtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGcc                                        <  1:3278511/100‑1 (MQ=255)

                                                      gtagtGGTCACGTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTCAGCAAAGTCTTATTATGTCTAACGTGcc                                        <  1:4906103/100‑1 (MQ=255)

                                                        agtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCcac                                      <  2:4814724/100‑1 (MQ=255)

                                                        agtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCcac                                      <  1:49904/100‑1 (MQ=255)

                                                        agtGGTCACTTCCGCTTAAATCTTGCGCAAACAGTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAGCGTGCcac                                      <  2:5125800/100‑1 (MQ=255)

                                                         gtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCcaca                                     >  1:2749851/1‑100 (MQ=255)

                                                         gtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCcaca                                     >  1:1080401/1‑100 (MQ=255)

                                                         gtGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCcaca                                     <  1:4042066/100‑1 (MQ=255)

                                                           ngTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATc                                   >  1:570751/2‑100 (MQ=255)

                                                           ggTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATc                                   >  2:3769022/1‑100 (MQ=255)

                                                           ggTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATc                                   <  2:3509450/100‑1 (MQ=255)

                                                            gTCACTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCa                                  <  1:920372/100‑1 (MQ=255)

                                                                cTTCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCg                              >  2:656696/1‑100 (MQ=255)

                                                                  tCCGCTTAAATCTTGCGCAAACATTTAACTCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCa                            >  2:2708606/1‑100 (MQ=255)

                                                                  tCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCa                            <  1:3920644/100‑1 (MQ=255)

                                                                  tCCGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCa                            <  2:3432631/100‑1 (MQ=255)

                                                                    cGCTTAAATCTTGCGCAAACATTTAACTCGTTTGGCGCCACTATCGCGGTTGTCTTCGCGCACAGCCCTATTTTGTCAAACGTGCCACATCACTCGCAAg                          >  2:1119910/1‑100 (MQ=255)

                                                                    cGCTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAg                          >  1:311203/1‑100 (MQ=255)

                                                                     gCTTAAATCGTGCGCAAACATTTAACGCGTATGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTGATGTTCTCTAACGTGCCACATCAATCGcccga                         >  2:3348940/1‑96 (MQ=255)

                                                                      cTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGAc                        >  2:3207346/1‑100 (MQ=255)

                                                                      cTTAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGAc                        <  1:2435602/100‑1 (MQ=255)

                                                                       ttAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACg                       <  2:2021790/100‑1 (MQ=255)

                                                                        tAAATCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGt                      >  2:1283678/1‑100 (MQ=255)

                                                                           aTCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtct                   <  1:2643268/100‑1 (MQ=255)

                                                                            tCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtctc                  >  2:5306914/1‑100 (MQ=255)

                                                                            tCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtctc                  <  1:624038/100‑1 (MQ=255)

                                                                            tCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtctc                  <  2:2536700/100‑1 (MQ=255)

                                                                            tCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtctc                  <  1:3661000/100‑1 (MQ=255)

                                                                            tCTTGCGCAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTtctc                  <  2:2278199/100‑1 (MQ=255)

                                                                                gcgcAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATa              <  2:1265152/100‑1 (MQ=255)

                                                                                  gcAAACATTTAACTCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATaaa            <  2:725310/100‑1 (MQ=255)

                                                                                  gcAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATaaa            <  1:2589193/100‑1 (MQ=255)

                                                                                   cAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATaaaa           >  2:412932/1‑100 (MQ=255)

                                                                                   cAAACATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATaaaa           <  1:2788662/100‑1 (MQ=255)

                                                                                       cATTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGtc       >  1:4251467/1‑100 (MQ=255)

                                                                                        aTTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGtct      <  1:1868618/100‑1 (MQ=255)

                                                                                        aTTTAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGtct      <  1:184219/100‑1 (MQ=255)

                                                                                          ttAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGTCTcc    <  1:3422411/100‑1 (MQ=255)

                                                                                          ttAACGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGTCTcc    >  2:615413/1‑100 (MQ=255)

                                                                                            aaCGCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTACCGCGCCACATCAATCGCAAGACGTTCTCGATAAAATGTCTCCag  >  2:2878315/1‑100 (MQ=255)

TAGGTTGTCTGGAAACTGCCGCAAACCCTTTTGTTACGGTATTAGGGCCGGAAAGTAGTGGTCACTTCCGCTTAAATCTTGCGCAAACATTTAACTCGTTTGGCGCAATTATCGCGGTTGTCTTTGGGCAAAGTCTTATTTTGTCTAACGTGCCACATCAATCGCAAGACGTTCTCGATAAAATGTCTCCAG  >  NC_000913/2934626‑2934817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`