BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,624	A→C	K287N (AAA→AAC)	ygeY →	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,624	0	A	C	97.7%	760.8 / 1.0	213	K287N (AAA→AAC)	ygeY	putative peptidase
Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (92/116); total (95/118)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAA  >  NC_000913/3009527‑3009720

 aGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCt                                                                                               <  1:1072034/100‑1 (MQ=255)

  gACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTa                                                                                              <  1:5037841/100‑1 (MQ=255)

   aCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTaa                                                                                             <  1:2112378/100‑1 (MQ=255)

    cAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAAc                                                                                            >  1:1844566/1‑100 (MQ=255)

     aGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAAcg                                                                                           <  2:4920197/100‑1 (MQ=255)

      gCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAAcgc                                                                                          >  1:3626830/1‑100 (MQ=255)

       cTGCGCAGTTTCAATCGACCGTCGTCTGACGTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAAcgcg                                                                                         >  2:215066/1‑100 (MQ=255)

        tGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCgg                                                                                        >  1:1186992/1‑100 (MQ=255)

        tGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCgg                                                                                        >  1:1485379/1‑100 (MQ=255)

         gcgcAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGgt                                                                                       <  1:3528314/100‑1 (MQ=255)

           gcAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGgttg                                                                                     <  2:2598524/100‑1 (MQ=255)

           gcAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGgttg                                                                                     <  2:1773897/100‑1 (MQ=255)

           gatctttCAATCGACCGTCGTCTGACCTGGCGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGgttg                                                                                     <  2:2503585/96‑1 (MQ=255)

              gTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGttt                                                                                  <  1:1415135/100‑1 (MQ=255)

               tttCAGTCGACCTTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCACTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTc                                                                                 <  2:4401285/100‑1 (MQ=255)

               tttCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTc                                                                                 <  1:4544166/100‑1 (MQ=255)

               tttCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTc                                                                                 >  1:2471565/1‑100 (MQ=255)

                ttCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCt                                                                                >  1:1048701/1‑100 (MQ=255)

                ttCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCt                                                                                >  1:3856764/1‑100 (MQ=255)

                ttCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGATTGTTTCt                                                                                >  1:5309645/1‑100 (MQ=255)

                ttCAATCGACCGTCCTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGgttgagatc                                                                                >  1:2528572/1‑95 (MQ=255)

                 tCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCtn                                                                               <  1:4415335/100‑2 (MQ=255)

                 tCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTa                                                                               <  1:1566992/100‑1 (MQ=255)

                   aaTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATg                                                                             <  2:1794227/100‑1 (MQ=255)

                   aaTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATg                                                                             <  2:2663620/100‑1 (MQ=255)

                   aaTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATg                                                                             >  2:4162766/1‑100 (MQ=255)

                   aaTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGAGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATg                                                                             <  1:4735579/100‑1 (MQ=255)

                    aTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGt                                                                            >  1:2620985/1‑100 (MQ=255)

                    aTCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGt                                                                            >  1:4023997/1‑100 (MQ=255)

                     tCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTa                                                                           <  1:4632578/100‑1 (MQ=255)

                           gtcgtcTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTa                                                                     <  2:1674292/100‑1 (MQ=255)

                           gtcgtcTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTa                                                                     <  1:5522145/100‑1 (MQ=255)

                            tcgtcTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTAc                                                                    <  1:4067548/100‑1 (MQ=255)

                             agtcTAACCTTGGTCGAAACCTGGGAAGGCGCGATTGACGAAATCCGCGCGCTGACTGCAGTACAGAACGCTATCTCGTTTGTTTCTATGTACAACTACg                                                                   <  2:4946299/99‑1 (MQ=255)

                                cTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAcc                                                                >  2:245870/1‑100 (MQ=255)

                                cTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAcc                                                                <  2:5222591/100‑1 (MQ=255)

                                  gACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAccgt                                                              >  1:830311/1‑100 (MQ=255)

                                    ccTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAccgtcc                                                            >  2:2008691/1‑100 (MQ=255)

                                     cTGGGGCGAAACCTGGGAAGGCGCGCTGGACGTAATCCACGCCCTGTCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAccgtccg                                                           >  1:4950967/1‑100 (MQ=255)

                                     cTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGAccgtccg                                                           >  2:3928201/1‑100 (MQ=255)

                                         tgCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGTTTGTTTCTATGTACACCTACGACCGTCCGTCCt                                                       >  2:2311802/2‑100 (MQ=255)

                                         ggagAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCt                                                       >  1:1703028/4‑100 (MQ=255)

                                         ggCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCt                                                       >  2:2387720/1‑100 (MQ=255)

                                          gCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTg                                                      >  2:1841460/1‑100 (MQ=255)

                                          gCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTg                                                      <  1:672464/100‑1 (MQ=255)

                                          gCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTg                                                      <  2:1350208/100‑1 (MQ=255)

                                           cGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTgg                                                     <  1:2567618/100‑1 (MQ=255)

                                             aaaCCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                   >  1:2493744/1‑100 (MQ=255)

                                             aaaCCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                   <  1:1858170/100‑1 (MQ=255)

                                                 cTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGc                                               >  1:1262746/1‑100 (MQ=255)

                                                  tGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGcc                                              <  1:4783853/100‑1 (MQ=255)

                                                   gggAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCt                                             >  2:1133871/1‑100 (MQ=255)

                                                      aaGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGt                                          <  1:1319152/100‑1 (MQ=255)

                                                       aGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGtt                                         >  1:3561304/1‑100 (MQ=255)

                                                       aGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGtt                                         >  2:4676182/1‑100 (MQ=255)

                                                        ggCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGttt                                        >  2:3730762/1‑100 (MQ=255)

                                                          cgcgcTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTAc                                      <  1:5389062/100‑1 (MQ=255)

                                                          cgcgcTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTAc                                      >  1:87270/1‑100 (MQ=255)

                                                          cgcgcTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTAc                                      <  2:1551434/100‑1 (MQ=255)

                                                               tGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAc                                 <  2:1474055/100‑1 (MQ=255)

                                                               tGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAc                                 <  2:5394279/100‑1 (MQ=255)

                                                               tGGACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAc                                 <  1:2315396/100‑1 (MQ=255)

                                                                ggACGAAATCCGCGCCGTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                <  2:3989225/100‑1 (MQ=255)

                                                                ggACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                >  2:4625005/1‑100 (MQ=255)

                                                                ggACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                >  2:3120043/1‑100 (MQ=255)

                                                                ggACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                <  2:819510/100‑1 (MQ=255)

                                                                ggACGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                >  1:1632717/1‑100 (MQ=255)

                                                                ggACGAAATCCGCACCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                >  1:3713414/1‑100 (MQ=255)

                                                                  aCGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGa                              >  1:2942173/1‑100 (MQ=255)

                                                                   cGAAATCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGaa                             >  2:2085158/1‑100 (MQ=255)

                                                                     taaTCTGCGCCCTTCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATg                           <  2:5121199/99‑1 (MQ=255)

                                                                     aaaTCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATg                           <  2:4086908/100‑1 (MQ=255)

                                                                      aaTCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGc                          >  2:332740/1‑100 (MQ=255)

                                                                        tCCGCGCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTa                        <  2:3677564/100‑1 (MQ=255)

                                                                          cgcgCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACt                      >  2:4765827/1‑100 (MQ=255)

                                                                          cgcgCCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACt                      >  1:4820969/1‑100 (MQ=255)

                                                                             gcCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTcc                   <  1:2411849/100‑1 (MQ=255)

                                                                             gcCCTGCCTGCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTcc                   <  2:2542829/100‑1 (MQ=255)

                                                                               cctgcctgCAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCg                 <  2:122812/100‑1 (MQ=255)

                                                                                 tgcctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTGCTCCCCGAc               >  1:5446331/1‑100 (MQ=255)

                                                                                 tgcctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGAc               <  2:2263140/100‑1 (MQ=255)

                                                                                 tgcctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGAc               >  1:3332995/1‑100 (MQ=255)

                                                                                 tgcctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGAc               <  1:4146665/100‑1 (MQ=255)

                                                                                   cctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCt             <  2:1018600/100‑1 (MQ=255)

                                                                                    ctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTg            >  2:2897833/1‑100 (MQ=255)

                                                                                    ctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTg            <  1:4224670/100‑1 (MQ=255)

                                                                                    ctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTg            <  2:3537690/100‑1 (MQ=255)

                                                                                    ctgcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTg            <  2:3034436/100‑1 (MQ=255)

                                                                                    ctgcAGTACAGAACGCTAAAGCGGTTGTTACTATATACCACTACGCCCGTCCGTCCTGGACTGGAATGGTATAACAAACAGAATGCTACTTCCCGACCTg            <  2:534856/100‑1 (MQ=255)

                                                                                      gcAGTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGa          >  2:4251175/1‑100 (MQ=255)

                                                                                         gTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAg       >  1:4885011/1‑100 (MQ=255)

                                                                                         gTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAg       <  2:4842519/100‑1 (MQ=255)

                                                                                         gTACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAg       >  1:1996336/1‑100 (MQ=255)

                                                                                          tACAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGt      <  1:3974295/100‑1 (MQ=255)

                                                                                           aCAGAACGCTAACGTGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTCCTTCCCGACCTGGAAAGTg     <  2:5038489/100‑1 (MQ=255)

                                                                                           aCAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTg     >  2:3275784/1‑100 (MQ=255)

                                                                                           aCAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTg     >  2:3153010/1‑100 (MQ=255)

                                                                                           aCAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCATGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTg     <  2:5342015/100‑1 (MQ=255)

                                                                                            cAGAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTgg    >  1:330544/1‑100 (MQ=255)

                                                                                              gAACGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCATGAATTACCCAACCGATTGCTACTTCCCGACCTGGAAAGTGgaa  >  2:5249911/1‑100 (MQ=255)

CAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAA  >  NC_000913/3009527‑3009720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`