BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,086,195	A→C	I3I (ATA→ATC)	yqgC →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,086,195	0	A	C	99.0%	672.1 / ‑2.3	192	I3I (ATA→ATC)	yqgC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (99/90); total (100/92)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 195 total aligned reads displayed.

AACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTC  >  NC_000913/3086098‑3086294

 aCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATcaca                                                                                                  <  1:97577/100‑1 (MQ=255)

   cACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATc                                                                                                >  2:1582383/1‑100 (MQ=255)

   cACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATc                                                                                                >  1:894595/1‑100 (MQ=255)

   cACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATc                                                                                                <  1:1509352/100‑1 (MQ=255)

            aCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTAt                                                                                       <  1:751684/100‑1 (MQ=255)

             ctctTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATg                                                                                      >  2:2589788/1‑100 (MQ=255)

              tctTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGc                                                                                     <  2:2288070/100‑1 (MQ=255)

               ctTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCa                                                                                    >  2:1218649/1‑100 (MQ=255)

                  gTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAg                                                                                 >  1:2148300/1‑100 (MQ=255)

                  gTTTCTACAAGAGCGCCCTTGTTCAGTACTCAGTAACTGTAACAAGTTCTTGAATCATGAGAAGCGACGAGATGTGTATCACATGGGGAGGTCTGCgaaa                                                                                 >  2:4642993/1‑99 (MQ=255)

                   tttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGc                                                                                <  1:3651214/100‑1 (MQ=255)

                   tttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGc                                                                                >  1:186174/1‑100 (MQ=255)

                   tttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGc                                                                                >  2:1319596/1‑100 (MQ=255)

                   tttttACAAAAGCGCCCTTGTTCAGTCCTCAGTAACTGTACCCAGCCCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGc                                                                                >  2:1509521/1‑100 (MQ=255)

                       tACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCagag                                                                            <  2:3095809/100‑1 (MQ=255)

                        aCAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGTGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAgaga                                                                           <  2:5115880/100‑1 (MQ=255)

                         cAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATGACATCGGCAGGTATGCAAAGCAGAGAt                                                                          >  2:537140/1‑100 (MQ=255)

                         cAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGAt                                                                          <  1:3016072/100‑1 (MQ=255)

                           agagCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGAtcg                                                                        >  2:460737/1‑98 (MQ=255)

                             agCGCCCTTGTTCGGGCCTCACTAACGGTAACCAGCTCGAGAATCCGGAGAAGAGCCGAGATGGGTATCACCTCGGCAGGTATGCAAAGCAGAGATGCag                                                                      <  2:5145962/100‑1 (MQ=255)

                              gcgcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCaga                                                                     >  1:3942674/1‑100 (MQ=255)

                               ngcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCagag                                                                    >  1:4417247/2‑100 (MQ=255)

                               cgcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCagag                                                                    >  2:2034117/1‑100 (MQ=255)

                               cgcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCagag                                                                    <  1:5160991/100‑1 (MQ=255)

                               cgcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCagag                                                                    <  1:4060567/100‑1 (MQ=255)

                                gcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGGTGGGTATCACATCGGCAGCTATGCAAAGCAGATATGCAGAGt                                                                   >  1:4851001/1‑100 (MQ=255)

                                gcCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGt                                                                   <  1:4543676/100‑1 (MQ=255)

                                 cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTg                                                                  >  2:3419167/1‑100 (MQ=255)

                                 cccTTGTTCAGGCCTCAGTAACTGTAACCAGTTCTTGAATCCTGAGACGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTg                                                                  <  2:1147644/100‑1 (MQ=255)

                                 cccTCGTTCAGTCCTCAGTAACCGTAACCAGCTCCTGAATCATGAGAAGCGCCGAGAAGGGCATCACATCGGAAGGTATGCCAAGCAGAGATGCagaccg                                                                  >  2:1103075/1‑97 (MQ=255)

                                  ccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGc                                                                 >  1:1980051/1‑100 (MQ=255)

                                   cTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCg                                                                >  2:1770423/1‑100 (MQ=255)

                                     tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCggg                                                              <  2:4823065/100‑1 (MQ=255)

                                     tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCggg                                                              <  2:1865391/100‑1 (MQ=255)

                                      gTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCgggg                                                             <  1:4955752/100‑1 (MQ=255)

                                      gTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCgggg                                                             <  1:2203420/100‑1 (MQ=255)

                                       ttCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGa                                                            >  2:4874533/1‑100 (MQ=255)

                                       ttCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGa                                                            >  2:2510881/1‑100 (MQ=255)

                                         cAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAAc                                                          <  1:816103/100‑1 (MQ=255)

                                         cAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAAc                                                          <  1:2521553/100‑1 (MQ=255)

                                         cAGTCCTCAGAAACTGTAACCAGCTCTTGAATCCGGAGAAGCGCCGAGCTGGGTATCACATAGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGAGGAAc                                                          >  2:2503846/1‑100 (MQ=255)

                                          actCCTCAGTAACTGTAACCAGCTCTTGAATACTGAGAAGCTCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACg                                                         <  2:525253/98‑1 (MQ=255)

                                          aGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACg                                                         >  2:1448983/1‑100 (MQ=255)

                                            tCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGaa                                                       <  1:2717693/100‑1 (MQ=255)

                                             ccTCAGTGACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAAt                                                      >  2:440794/1‑100 (MQ=255)

                                               tCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCt                                                    >  1:1813859/1‑100 (MQ=255)

                                               tCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCt                                                    >  1:3694675/1‑100 (MQ=255)

                                                 aGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTc                                                  <  1:1313665/100‑1 (MQ=255)

                                                  gTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCa                                                 >  2:2972628/1‑100 (MQ=255)

                                                  gTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCa                                                 >  1:340432/1‑100 (MQ=255)

                                                   gatcTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCAc                                                <  2:694320/97‑1 (MQ=255)

                                                    aaCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCAcc                                               >  2:4017541/1‑100 (MQ=255)

                                                    aaCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCAcc                                               <  2:4952659/100‑1 (MQ=255)

                                                     aCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCa                                              >  2:1437060/1‑100 (MQ=255)

                                                     aCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCa                                              >  1:1031001/1‑100 (MQ=255)

                                                     aCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCa                                              >  2:852563/1‑100 (MQ=255)

                                                     aCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGAAGAGATGCAGAGTGAGGGGAACGAATATTCACCa                                              >  1:1031000/1‑100 (MQ=255)

                                                      cTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAg                                             >  1:4281668/1‑100 (MQ=255)

                                                       tGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGa                                            >  2:630805/1‑100 (MQ=255)

                                                       tGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGa                                            >  1:552315/1‑100 (MQ=255)

                                                        gTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCAcagatc                                           >  1:702937/1‑95 (MQ=255)

                                                         tAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAAc                                          <  2:1442764/100‑1 (MQ=255)

                                                         tAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAAc                                          >  1:4092316/1‑100 (MQ=255)

                                                           aCCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACgg                                        >  1:5406490/1‑100 (MQ=255)

                                                             cAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTg                                      >  1:3120172/1‑100 (MQ=255)

                                                              aGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTga                                     <  1:1044723/100‑1 (MQ=255)

                                                                  ctTGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACa                                 >  2:3153495/1‑100 (MQ=255)

                                                                    tGAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAgg                               <  1:5014587/100‑1 (MQ=255)

                                                                     gAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGt                              <  2:1021387/100‑1 (MQ=255)

                                                                     gAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGt                              <  1:4710596/100‑1 (MQ=255)

                                                                     gAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGt                              >  1:2190879/1‑100 (MQ=255)

                                                                     gAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGt                              >  2:1311027/1‑100 (MQ=255)

                                                                     gAATCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGt                              >  1:3222435/1‑100 (MQ=255)

                                                                       aTCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTa                            >  1:1373537/1‑100 (MQ=255)

                                                                       aTCCTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTa                            <  2:3002795/100‑1 (MQ=255)

                                                                         ccTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAag                          <  1:365820/100‑1 (MQ=255)

                                                                          cTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCTGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAagc                         <  1:3307654/100‑1 (MQ=255)

                                                                          cTGAGAAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAagc                         >  1:1617683/1‑100 (MQ=255)

                                                                          cTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAagc                         <  1:379787/100‑1 (MQ=255)

                                                                          cTGAGAAGAGCCGAGACGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAagc                         >  2:5314341/1‑100 (MQ=255)

                                                                             agaAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAagcagc                      <  2:4042448/100‑1 (MQ=255)

                                                                              gaAGCGCCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAgcagca                     <  1:4152976/100‑1 (MQ=255)

                                                                                   gcCGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACaa                >  1:289055/1‑100 (MQ=255)

                                                                                    ccGAGATGGGTACCCCATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCGGACCGGTGAGAAAGGTTAAGCAGCAGACAAc               >  2:2961579/1‑100 (MQ=255)

                                                                                     cGAGATGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACg              >  1:138666/1‑100 (MQ=255)

                                                                                      gagaTGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACgg             <  2:3660650/100‑1 (MQ=255)

                                                                                      gagaTGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACgg             >  1:3495193/1‑100 (MQ=255)

                                                                                      atcttGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGGAGCAGACAACgg             <  1:4783679/96‑1 (MQ=255)

                                                                                          tGGGTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCa         >  2:3219153/1‑100 (MQ=255)

                                                                                          tGGGTATCACATCAGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCa         >  2:4987008/1‑100 (MQ=255)

                                                                                           gggTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCat        <  1:1337228/100‑1 (MQ=255)

                                                                                           gggTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCat        >  2:2678687/1‑100 (MQ=255)

                                                                                           gggTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCat        >  2:2136688/1‑100 (MQ=255)

                                                                                           gggTATCACAACGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCat        >  2:3805748/1‑100 (MQ=255)

                                                                                            ggTATCACATCGGCAGGTATGCAAGGCAGCGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTGAGCAGCAGACAACGGTTCatt       >  1:5195738/1‑100 (MQ=255)

                                                                                            ggTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCatt       >  2:937893/1‑100 (MQ=255)

                                                                                             gTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCatta      >  1:1176793/1‑100 (MQ=255)

                                                                                             gTATCACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCatta      >  1:1748380/1‑100 (MQ=255)

                                                                                                taACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTAttt   <  2:3373636/100‑1 (MQ=255)

                                                                                                 aaCATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTc  <  1:1266407/100‑1 (MQ=255)

AACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTC  >  NC_000913/3086098‑3086294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`