BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,170	T→G	V296G (GTC→GGC)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,170	0	T	G	99.0%	700.4 / ‑4.8	196	V296G (GTC→GGC)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (91/102); total (94/102)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

TCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGG  >  NC_000913/3089074‑3089265

tCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCgg                                                                                              <  2:2666725/100‑1 (MQ=255)

 cACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTggcggc                                                                                             <  2:386968/100‑1 (MQ=255)

 cACCGGGATGAACCTCATCATGTATTACGCGACGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTggcggc                                                                                             <  2:4299283/100‑1 (MQ=255)

  aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                            <  1:1000274/100‑1 (MQ=255)

  aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                            <  2:3382055/100‑1 (MQ=255)

  aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                            <  1:3796085/100‑1 (MQ=255)

  aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                            <  1:3324735/100‑1 (MQ=255)

  aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAACGTTCGACCTGGCTTGTTATACCAACGCGACCGAGCAAATGTTAGGGATCGTCATTGGCgggc                                                                                            <  2:2143362/100‑3 (MQ=255)

      ggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGAc                                                                                        <  1:4144651/100‑1 (MQ=255)

       gATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTCTACCAACACTCCCGAGCAAATGTGGGGGGCCGTGATTGGCGGCCTGAcc                                                                                       >  1:4519848/1‑100 (MQ=255)

        aTGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGACGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCa                                                                                      >  1:4737464/1‑100 (MQ=255)

         tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCaa                                                                                     >  1:3733357/1‑100 (MQ=255)

         tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCaa                                                                                     >  2:2515337/1‑100 (MQ=255)

         tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCaa                                                                                     >  1:3404459/1‑100 (MQ=255)

         tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCaa                                                                                     >  1:1536498/1‑100 (MQ=255)

           aaCGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACg                                                                                   >  1:1280698/1‑100 (MQ=255)

            aCGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGt                                                                                  >  1:5296257/1‑100 (MQ=255)

             cGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTa                                                                                 >  2:1705073/1‑100 (MQ=255)

              gTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAAAGTAc                                                                                <  2:4674372/100‑1 (MQ=255)

                 atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACttn                                                                             <  1:512684/100‑2 (MQ=255)

                 atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                             >  1:280076/1‑100 (MQ=255)

                 atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                             >  2:3320234/1‑100 (MQ=255)

                 atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                             <  1:3270406/100‑1 (MQ=255)

                 atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                             <  1:3745413/100‑1 (MQ=255)

                     tGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCAc                                                                         <  2:2296418/100‑1 (MQ=255)

                      gTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCAcc                                                                        <  2:2974340/100‑1 (MQ=255)

                      gTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGAGACCGTGATTGGCGGCCTGACCAACGTACTTGCCAcc                                                                        >  2:4997415/1‑100 (MQ=255)

                       tATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCt                                                                       <  1:4055402/100‑1 (MQ=255)

                         ttACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGCACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCttt                                                                     <  2:4223972/100‑1 (MQ=255)

                          tACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGGACTGGCGAGCTTTa                                                                    >  1:1648255/1‑100 (MQ=255)

                           aCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTAt                                                                   <  2:319594/100‑1 (MQ=255)

                            cgcgCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATc                                                                  >  2:1897174/1‑100 (MQ=255)

                            cgcgCCGAAAATCTTCGAACTGGAGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATc                                                                  >  2:4617484/1‑100 (MQ=255)

                             gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCg                                                                 >  2:2201499/1‑100 (MQ=255)

                             gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCg                                                                 <  2:4769585/100‑1 (MQ=255)

                                 ngAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                             >  1:4431364/2‑100 (MQ=255)

                                 cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                             >  1:700919/1‑100 (MQ=255)

                                    aaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCgg                                                          >  1:3151884/1‑100 (MQ=255)

                                    aaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCgg                                                          <  2:5259952/100‑1 (MQ=255)

                                    aaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCgg                                                          <  2:1936338/100‑1 (MQ=255)

                                       tCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCt                                                       <  1:299568/100‑1 (MQ=255)

                                        cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                      >  2:647008/1‑100 (MQ=255)

                                        cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                      <  2:1216379/100‑1 (MQ=255)

                                        cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                      >  1:3491037/1‑100 (MQ=255)

                                        cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                      <  2:2769424/100‑1 (MQ=255)

                                        cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                      >  1:28203/1‑100 (MQ=255)

                                         ttCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttg                                                     <  2:17640/100‑1 (MQ=255)

                                          tCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgt                                                    <  1:4225497/100‑1 (MQ=255)

                                           cGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgtt                                                   >  1:87861/1‑100 (MQ=255)

                                            gAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgttg                                                  >  1:4356030/1‑100 (MQ=255)

                                            gAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgttg                                                  >  1:4301260/1‑100 (MQ=255)

                                            gAACTGGCGCGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgttg                                                  >  1:5147512/1‑100 (MQ=255)

                                             aaCTGGCGGGTTATCCCAACACTACCGAGCAAATGTGGGGGACCGTGCTTGCCGGCATGACAAACATACTTGCCACCTTTATAGCAATCGACATTGTTGa                                                 >  2:5484760/1‑100 (MQ=255)

                                              aCTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAc                                                >  1:1722135/1‑100 (MQ=255)

                                               cTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAcc                                               >  1:1675346/1‑100 (MQ=255)

                                               cTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAcc                                               <  2:1274868/100‑1 (MQ=255)

                                                tGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCg                                              <  1:3649527/100‑1 (MQ=255)

                                                tGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCg                                              >  1:1917974/1‑100 (MQ=255)

                                                 ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGc                                             >  1:2406472/1‑100 (MQ=255)

                                                   cGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTg                                           <  2:1753489/100‑1 (MQ=255)

                                                    gggTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgg                                          >  1:118684/1‑100 (MQ=255)

                                                     ggTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTggg                                         <  1:3580889/100‑1 (MQ=255)

                                                     ggTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTggg                                         >  1:2691933/1‑100 (MQ=255)

                                                      gTTATACCAACCCTACCGAGAAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgggg                                        <  2:3632092/100‑1 (MQ=255)

                                                      gTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgggg                                        <  1:2431882/100‑1 (MQ=255)

                                                      gTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgggg                                        >  2:3371377/1‑100 (MQ=255)

                                                      gTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgggg                                        <  1:2078228/100‑1 (MQ=255)

                                                         ataCCAACACTACCGAGTAAATGTGGGGGAGCGTGATTGGCGACCTAACCAACGTACTTGCCACCTTTATCGTCATCGGCTTTTTTGAACGCGGGGGACg                                     <  2:837195/100‑1 (MQ=255)

                                                           aCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTa                                   <  1:2022597/100‑1 (MQ=255)

                                                           aCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTa                                   <  1:3386074/100‑1 (MQ=255)

                                                           aCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTa                                   <  2:3445620/100‑1 (MQ=255)

                                                               acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTCCTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                               <  2:3593678/100‑1 (MQ=255)

                                                               acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGTACGTAAAcc                               <  2:4546668/100‑1 (MQ=255)

                                                                  cTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaac                            <  1:4538906/100‑1 (MQ=255)

                                                                   tACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacg                           <  1:3781134/100‑1 (MQ=255)

                                                                   tACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCACTGGGGACGTAAACCaacg                           <  1:4284269/100‑1 (MQ=255)

                                                                    aCCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgc                          <  2:1655551/100‑1 (MQ=255)

                                                                     ccGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgct                         <  2:2255704/100‑1 (MQ=255)

                                                                     ccGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgct                         >  2:28879/1‑100 (MQ=255)

                                                                     ccGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgct                         >  2:446320/1‑100 (MQ=255)

                                                                       gAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaa                       >  2:2523090/1‑100 (MQ=255)

                                                                        aacAAATGTGGGCGACCGTGATTGGCGCCCTGACCAACGTACCTGCCAGCTTTATCGCAACCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                      <  1:5299765/98‑1 (MQ=255)

                                                                        aGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                      <  1:3994604/100‑1 (MQ=255)

                                                                         gCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacg                     <  2:1083038/100‑1 (MQ=255)

                                                                          cAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacgc                    >  1:5426867/1‑100 (MQ=255)

                                                                            aaTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTg                  >  1:2621115/1‑100 (MQ=255)

                                                                             aTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTgg                 <  2:425160/100‑1 (MQ=255)

                                                                             aTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTgg                 <  2:5063698/100‑1 (MQ=255)

                                                                                tgGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCt              >  2:2615179/1‑100 (MQ=255)

                                                                                   gggACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTcc           <  1:4308676/100‑1 (MQ=255)

                                                                                    ggACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCt          <  1:2640895/100‑1 (MQ=255)

                                                                                     gACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTg         >  2:3262017/1‑100 (MQ=255)

                                                                                      aCCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTgg        <  1:1013032/100‑1 (MQ=255)

                                                                                       ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt       <  2:3789607/100‑1 (MQ=255)

                                                                                       ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt       <  1:4712342/100‑1 (MQ=255)

                                                                                        cGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTg      <  2:2044772/100‑1 (MQ=255)

                                                                                        cGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTg      >  1:764794/1‑100 (MQ=255)

                                                                                          tGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt    <  1:2471711/100‑1 (MQ=255)

                                                                                          tGATTGGCGGACTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt    <  2:1146238/100‑1 (MQ=255)

                                                                                            aTTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATgg  >  2:4740687/1‑100 (MQ=255)

TCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGG  >  NC_000913/3089074‑3089265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`