Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,594 T→G A10A (GCA→GCC yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,5940TG99.0% 717.2 / NA 205A10A (GCA→GCCyggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base G (132/69);  minor base A (0/2);  total (133/72)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT  >  NC_000913/3101500‑3101690
                                                                                              |                                                                                                
tCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggctgc                                                                                             >  1:341498/1‑100 (MQ=255)
  aCCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccg                                                                                           >  2:1919470/1‑100 (MQ=255)
   ccTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgc                                                                                          >  1:4728192/1‑100 (MQ=255)
   ccTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgc                                                                                          >  2:3041843/1‑100 (MQ=255)
   ccTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgc                                                                                          >  2:2798210/1‑100 (MQ=255)
   ccTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGGCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgc                                                                                          >  1:437886/1‑100 (MQ=255)
    cTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgcc                                                                                         >  2:494122/1‑100 (MQ=255)
    cTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgcc                                                                                         <  1:2685773/100‑1 (MQ=255)
    cTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgcc                                                                                         >  2:2062258/1‑100 (MQ=255)
     tGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCca                                                                                        <  2:2425868/100‑1 (MQ=255)
      gCACGGTTTGCGGGCTGACAATCACATCGTCCCGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcag                                                                                       <  2:2256029/100‑1 (MQ=255)
      gCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcag                                                                                       >  2:1320274/1‑100 (MQ=255)
       cACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagc                                                                                      <  2:1264669/100‑1 (MQ=255)
        aCGGTTTTCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagca                                                                                     >  2:2197135/1‑100 (MQ=255)
        aCGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagca                                                                                     >  2:1492365/1‑100 (MQ=255)
         tggTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagcag                                                                                    >  1:5064933/2‑100 (MQ=255)
         cGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagcag                                                                                    >  1:589063/1‑100 (MQ=255)
         cGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagcag                                                                                    <  2:420396/100‑1 (MQ=255)
          ggTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCagcagc                                                                                   >  1:4760339/1‑100 (MQ=255)
          ggTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCagcagc                                                                                   >  2:412652/1‑100 (MQ=255)
           gTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAgcagca                                                                                  >  1:4223251/1‑100 (MQ=255)
                cGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTg                                                                             <  2:3171109/100‑1 (MQ=255)
                 gggCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgc                                                                            >  1:1140313/1‑100 (MQ=255)
                  ggCTGAGAATCACATCGTCACGCGGCGTGAGGCTGCACTGGTAGTAGGCGAGAGCGGTCATTGCCGACACTGAAAGGGCTGCCGCCAGCAGCAGTCTgcg                                                                           <  2:5171599/100‑1 (MQ=255)
                  ggCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcg                                                                           >  1:163714/1‑100 (MQ=255)
                   gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcgc                                                                          <  2:4756662/100‑1 (MQ=255)
                    cTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGca                                                                         <  1:1267585/100‑1 (MQ=255)
                    cTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGca                                                                         <  2:3834221/100‑1 (MQ=255)
                     tGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcat                                                                        >  1:3439535/1‑100 (MQ=255)
                     tGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGACAGCAGCATTTTGCGcat                                                                        <  1:205139/100‑1 (MQ=255)
                      gACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatc                                                                       >  1:5263137/1‑100 (MQ=255)
                        cAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCTGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatcat                                                                     >  2:5375137/1‑100 (MQ=255)
                        cAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatcat                                                                     >  2:755876/1‑100 (MQ=255)
                        cAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatcat                                                                     >  1:2606188/1‑100 (MQ=255)
                           tCACATTGTGACGCGGCGGGACGCTGCACTGGTAATCGGCGTGAGCGGTCATTGCCGTCACTTAAAGGGCTGCCGCCAGCAGCATTTTGCACATCATAGt                                                                  >  2:2532270/1‑100 (MQ=255)
                           tCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGt                                                                  <  1:4304365/100‑1 (MQ=255)
                             acaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCt                                                                <  1:2825168/100‑1 (MQ=255)
                              cagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCtt                                                               >  1:1920312/4‑100 (MQ=255)
                              caTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCtt                                                               >  1:3290513/1‑100 (MQ=255)
                               aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTc                                                              <  2:4658197/100‑1 (MQ=255)
                                  gTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCt                                                           >  1:2738883/1‑100 (MQ=255)
                                    cACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCa                                                         <  2:3676514/100‑1 (MQ=255)
                                      cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                       >  1:902072/1‑100 (MQ=255)
                                      cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                       <  2:5088839/100‑1 (MQ=255)
                                         ggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaa                                                    <  2:2933670/100‑1 (MQ=255)
                                          gcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaa                                                   >  2:3764138/1‑100 (MQ=255)
                                           cgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaa                                                  >  1:557977/1‑100 (MQ=255)
                                            gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaaa                                                 >  1:692235/1‑100 (MQ=255)
                                            gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaaa                                                 <  1:3777648/100‑1 (MQ=255)
                                             tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAAt                                                >  2:924425/1‑100 (MQ=255)
                                             tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAAt                                                >  1:3549529/1‑100 (MQ=255)
                                                cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAg                                             >  1:2131527/1‑100 (MQ=255)
                                                  cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                           >  1:5545487/1‑100 (MQ=255)
                                                   tgtaCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGcc                                          >  2:193334/4‑100 (MQ=255)
                                                     naCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAg                                        >  1:4416407/2‑100 (MQ=255)
                                                     cACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAg                                        <  1:1183726/100‑1 (MQ=255)
                                                       cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                      >  2:1310104/1‑100 (MQ=255)
                                                       cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCGGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                      >  2:5425982/1‑100 (MQ=255)
                                                         ggTCGTCGTCTTAAGCGGCCATTGCCGCCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGCCTTCCCTGAAGCAAAACTCAGGCCAGCAtt                                    >  2:2032610/1‑100 (MQ=255)
                                                         ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAtt                                    >  2:608434/1‑100 (MQ=255)
                                                           tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTg                                  >  1:3157713/1‑100 (MQ=255)
                                                            aGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                 >  1:4145294/1‑100 (MQ=255)
                                                             gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                >  2:1855378/1‑100 (MQ=255)
                                                             gTCGGCATGAGCGGTCATCGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                >  1:4640876/1‑100 (MQ=255)
                                                              tCGGCGTGAGCGGTCATTGCCGTCACTGAAGGGGCTGCCGACAGCAGCATTTTGCGCATCATAGTAAAAAAAAAAAAAAAAATCAGGCCAGCATTTGCTg                               <  2:2076642/100‑1 (MQ=255)
                                                               cGGCGTGAGCGGTCATTGCCGTCACAGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTACTgg                              >  1:4991193/1‑100 (MQ=255)
                                                                ggCGTAAACGGTCCTTGCCGTCACTGAAACGGCTGCCGCCAGCAGCACTTTGCGCATCATAGTCTTCCCTCAACACAAAACCAGGCCCGCCTTAGCTGGc                             >  2:4531006/1‑100 (MQ=255)
                                                                 gCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcc                            <  2:356005/100‑1 (MQ=255)
                                                                  cGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGccc                           >  2:1727483/1‑100 (MQ=255)
                                                                   gTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCTCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCATGCCAGCAAATGCTGGcctg                          <  2:1127341/100‑3 (MQ=255)
                                                                   gTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcccc                          >  1:1383709/1‑100 (MQ=255)
                                                                    tGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctga                         <  2:433133/100‑4 (MQ=255)
                                                                      aGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                       >  1:3723646/1‑100 (MQ=255)
                                                                      aGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctgatt                       <  2:3788926/100‑6 (MQ=255)
                                                                      aGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCAGTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                       <  2:4586605/100‑1 (MQ=255)
                                                                         ggTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg                    >  1:215593/1‑100 (MQ=255)
                                                                         ggTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg                    <  2:348306/100‑1 (MQ=255)
                                                                         ggTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg                    <  2:2639530/100‑1 (MQ=255)
                                                                           tCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAt                  <  1:1240820/100‑1 (MQ=255)
                                                                            cATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa                 >  1:3602941/1‑100 (MQ=255)
                                                                            cATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa                 >  1:2948915/1‑100 (MQ=255)
                                                                               ctccGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACa              <  2:2840789/98‑1 (MQ=255)
                                                                                 ccGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa            >  1:1416494/1‑100 (MQ=255)
                                                                                  cGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg           >  1:3050356/1‑100 (MQ=255)
                                                                                  cGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg           >  2:1489910/1‑100 (MQ=255)
                                                                                   gTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt          <  1:102161/100‑1 (MQ=255)
                                                                                   gTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATAATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGCTTGATAACACAGt          >  1:4253215/1‑100 (MQ=255)
                                                                                    gatcTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGGTAACAAAGTa         <  2:5070276/97‑1 (MQ=255)
                                                                                     cACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc        >  2:4914434/1‑100 (MQ=255)
                                                                                     cACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc        <  2:3595960/100‑1 (MQ=255)
                                                                                      aCTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg       >  2:4481352/1‑100 (MQ=255)
                                                                                       cTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc      >  1:5409347/1‑100 (MQ=255)
                                                                                       cTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc      >  1:938604/1‑100 (MQ=255)
                                                                                        tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg     >  1:176366/1‑100 (MQ=255)
                                                                                        tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg     >  1:887937/1‑100 (MQ=255)
                                                                                        tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg     <  2:852307/100‑1 (MQ=255)
                                                                                        tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg     >  2:3355805/1‑100 (MQ=255)
                                                                                         gAAAGGGCTGCCGCCGGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCGCAGATTGATAACAGAGTACGCGt    >  1:4849554/1‑100 (MQ=255)
                                                                                         gAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAGAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt    >  2:1537342/1‑100 (MQ=255)
                                                                                         gAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTaagatc    >  1:5141032/1‑95 (MQ=255)
                                                                                              |                                                                                                
TCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT  >  NC_000913/3101500‑3101690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: