BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,612	A→C	L262R (CTG→CGG)	parE ←	DNA topoisomerase IV, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,612	0	A	C	100.0%	729.5 / NA	200	L262R (CTG→CGG)	parE	DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (104/96); total (104/96)

Only 100 of 206 total aligned reads displayed.

GAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCA  >  NC_000913/3174515‑3174708

gAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAATAACCCGAGTACCGCCCTAAACCGCCGGGACAGGCTTAACGTAGCTTTCGGTcagcact                                                                                                >  2:1060063/1‑98 (MQ=255)

 aaCTCACGCATCGCGTCCAACAGGCCCTGACGTAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGtt                                                                                               <  1:5097300/100‑1 (MQ=255)

 aaCTCACGCATCGCGTCCAACAGGCCCTGACGCAGGCCATTAACATGGGTAACGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGtt                                                                                               <  1:2101116/100‑1 (MQ=255)

 aaCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGtt                                                                                               <  1:3258671/100‑1 (MQ=255)

   cTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCa                                                                                             >  2:2212099/1‑100 (MQ=255)

    tCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAc                                                                                            <  1:920936/100‑1 (MQ=255)

     cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                           >  2:2501218/1‑100 (MQ=255)

     cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                           >  2:3905050/1‑100 (MQ=255)

     cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                           >  2:3215872/1‑100 (MQ=255)

      aCGCATCGCGTCCAACAGGCCCTGGCGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTGGACGTAGCTTTCGGTCAGCCGTTCACCg                                                                                          <  1:1209907/100‑1 (MQ=255)

       cGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGc                                                                                         >  1:4228442/1‑100 (MQ=255)

       cGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGc                                                                                         >  1:1125824/1‑100 (MQ=255)

        gCATCACGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGcc                                                                                        <  2:1774548/100‑1 (MQ=255)

          aTCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTTCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACGGCCtt                                                                                      <  1:4997193/100‑1 (MQ=255)

          aTCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCtt                                                                                      >  2:4458315/1‑100 (MQ=255)

          aTCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCtt                                                                                      >  1:2563949/1‑100 (MQ=255)

          aTCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCtt                                                                                      >  2:877896/1‑100 (MQ=255)

            cgcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTcc                                                                                    <  1:377292/100‑1 (MQ=255)

             gcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCg                                                                                   <  1:3576823/100‑1 (MQ=255)

               gTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                 <  2:141737/100‑1 (MQ=255)

                tCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCa                                                                                >  2:2024140/1‑100 (MQ=255)

                 ccAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAg                                                                               >  1:2141215/1‑100 (MQ=255)

                  cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                              >  2:498522/1‑100 (MQ=255)

                  cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                              >  2:912945/1‑100 (MQ=255)

                  cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                              <  1:1057422/100‑1 (MQ=255)

                   aaCAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGcc                                                                             <  2:944316/100‑1 (MQ=255)

                   aaCAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGcc                                                                             <  1:181805/100‑1 (MQ=255)

                     cAGTCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcac                                                                           <  2:4613736/100‑1 (MQ=255)

                      aGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcaca                                                                          >  1:5460762/1‑100 (MQ=255)

                       ggCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAg                                                                         >  2:66304/1‑100 (MQ=255)

                          ccTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTAAGCCGTTCACCGCCGTCCGGCAGCCACAGTAg                                                                      >  1:5117324/1‑100 (MQ=255)

                           cTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgc                                                                     >  2:2069104/1‑100 (MQ=255)

                            tGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgcg                                                                    >  1:3983642/1‑100 (MQ=255)

                            tGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgcg                                                                    <  1:3692374/100‑1 (MQ=255)

                               cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                 <  2:1214385/100‑1 (MQ=255)

                               cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                 >  2:3960507/1‑100 (MQ=255)

                               cGCAGACCATTAACATGGGTACCGCCCTGCATCGGTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                 >  2:5075829/1‑100 (MQ=255)

                                 cAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAg                                                               >  2:3248609/1‑100 (MQ=255)

                                 cAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAg                                                               <  1:198618/100‑1 (MQ=255)

                                   gACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTc                                                             >  1:2635616/1‑100 (MQ=255)

                                    aCCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTcc                                                            <  2:3535445/100‑1 (MQ=255)

                                     ccATTAAGACGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTGAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                           >  2:4527558/1‑100 (MQ=255)

                                        ttAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAg                                                        <  2:2567306/100‑1 (MQ=255)

                                        ttAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAg                                                        <  2:730254/100‑1 (MQ=255)

                                         tAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                       <  2:4946601/100‑1 (MQ=255)

                                         tAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                       <  1:5328602/100‑1 (MQ=255)

                                          aaCATGGGTACCGCCCTGCGTAGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCt                                                      >  1:4750429/1‑100 (MQ=255)

                                          aaCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCt                                                      >  2:1456544/1‑100 (MQ=255)

                                           aCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCtt                                                     <  1:745585/100‑1 (MQ=255)

                                           aCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCtt                                                     >  2:340794/1‑100 (MQ=255)

                                            cATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTc                                                    >  1:1280509/1‑100 (MQ=255)

                                              tGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAg                                                  >  2:591717/1‑100 (MQ=255)

                                              tGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAg                                                  <  1:47263/100‑1 (MQ=255)

                                                    ccGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAc                                            >  2:2405559/1‑100 (MQ=255)

                                                     cGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAcc                                           >  2:2645006/1‑100 (MQ=255)

                                                     cGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAcc                                           >  2:1000913/1‑100 (MQ=255)

                                                      gCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCATTATCACCa                                          <  2:5276791/100‑1 (MQ=255)

                                                       cccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCTTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAg                                         <  1:785619/100‑1 (MQ=255)

                                                         ntGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCg                                       >  1:4447555/2‑100 (MQ=255)

                                                         cTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCg                                       <  1:1698539/100‑1 (MQ=255)

                                                          tGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGa                                      >  2:929845/1‑100 (MQ=255)

                                                           gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGaa                                     >  1:3723075/1‑100 (MQ=255)

                                                           gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGaa                                     <  2:1110807/100‑1 (MQ=255)

                                                            cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGaaa                                    >  2:1822698/1‑100 (MQ=255)

                                                                gTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTAc                                >  2:1964319/1‑100 (MQ=255)

                                                                gTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTAc                                <  1:942325/100‑1 (MQ=255)

                                                                 ttGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTAcc                               <  2:466743/100‑1 (MQ=255)

                                                                 ttGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTAcc                               <  1:1455723/100‑1 (MQ=255)

                                                                  tGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCg                              <  1:4552072/100‑1 (MQ=255)

                                                                  tGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCg                              <  2:5387612/100‑1 (MQ=255)

                                                                    ggATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCCCAGCTTCAGTATCACCACCGAAATTACCGAt                            >  2:4306415/1‑100 (MQ=255)

                                                                    ggATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGAt                            >  1:3313031/1‑100 (MQ=255)

                                                                     gATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATa                           <  2:3069117/100‑1 (MQ=255)

                                                                      aTAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATaa                          >  1:5243654/1‑100 (MQ=255)

                                                                      aTAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATaa                          >  1:490394/1‑100 (MQ=255)

                                                                         aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACg                       <  1:2249323/100‑1 (MQ=255)

                                                                         aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACg                       <  1:2361012/100‑1 (MQ=255)

                                                                         aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACg                       >  1:3319763/1‑100 (MQ=255)

                                                                          ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATagatcg                      >  2:3642992/1‑95 (MQ=255)

                                                                          ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                      >  2:1318282/1‑100 (MQ=255)

                                                                          ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                      >  2:2795009/1‑100 (MQ=255)

                                                                          ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                      <  2:2157021/100‑1 (MQ=255)

                                                                          ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                      >  2:1397462/1‑100 (MQ=255)

                                                                               aCGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                 <  2:1736987/100‑1 (MQ=255)

                                                                               aCGTAGCTGTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                 <  2:235316/100‑1 (MQ=255)

                                                                                 gTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTcc               <  1:4603419/100‑1 (MQ=255)

                                                                                  tAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCg              <  2:2191653/100‑1 (MQ=255)

                                                                                    gCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGc            <  1:3428670/100‑1 (MQ=255)

                                                                                    gCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGc            <  1:4705642/100‑1 (MQ=255)

                                                                                    gCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGc            <  1:2688314/100‑1 (MQ=255)

                                                                                      tttCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAg          <  2:1724497/100‑1 (MQ=255)

                                                                                          ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTc      <  2:5163509/100‑1 (MQ=255)

                                                                                          ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTc      >  2:5341073/1‑100 (MQ=255)

                                                                                          ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTc      >  1:1609712/1‑100 (MQ=255)

                                                                                           gTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCg     >  1:1863201/1‑100 (MQ=255)

                                                                                           gTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCg     >  1:3901500/1‑100 (MQ=255)

                                                                                             cagccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGc   <  1:5216691/100‑1 (MQ=255)

                                                                                             cagccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGc   <  2:1734137/100‑1 (MQ=255)

                                                                                             cagccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGc   <  2:1567714/100‑1 (MQ=255)

                                                                                              agccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCa  >  1:961954/1‑100 (MQ=255)

GAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCA  >  NC_000913/3174515‑3174708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`