Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,197,496 T→G T716P (ACT→CCT)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,197,4960TG98.8% 576.0 / NA 168T716P (ACT→CCT) glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  major base G (87/77);  minor base A (1/1);  total (90/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

AAAATGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGC  >  NC_000913/3197399‑3197592
                                                                                                 |                                                                                                
caaaTGTCGGAAGAGGTAGGCGTACGAAACAGTTGCATAATGCGTTTCGCCACACGCTGATAAAATTGCCGCCCGTCGATTTCCCGCTCACCgtcaggca                                                                                                <  2:5077288/99‑1 (MQ=255)
    tctcGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGc                                                                                            <  2:3969689/97‑1 (MQ=255)
     gCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCACTCACCGTCAGGCATCGCa                                                                                           >  2:3160824/1‑100 (MQ=255)
       cGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGgcatcgcatc                                                                                         <  2:1865548/100‑1 (MQ=255)
       cGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGgcatcgcatc                                                                                         >  2:1618965/1‑100 (MQ=255)
         gAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCa                                                                                       <  2:1956262/100‑1 (MQ=255)
            gagGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTg                                                                                    <  2:496093/100‑1 (MQ=255)
              ggTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTggg                                                                                  >  1:2204034/1‑100 (MQ=255)
              ggTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTggg                                                                                  >  2:1728375/1‑100 (MQ=255)
              ggTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCCTCGCTTTCCCGCTAACCGGCAGGCAGCGCCGCCAtgagg                                                                                  >  2:4701623/1‑96 (MQ=255)
               gTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGc                                                                                 <  2:1344807/100‑1 (MQ=255)
                tACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCa                                                                                >  2:5121849/1‑100 (MQ=255)
                 aCGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCaa                                                                               >  2:1299270/1‑100 (MQ=255)
                   gcgTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATc                                                                             >  2:1437442/1‑100 (MQ=255)
                      tACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATg                                                                          >  2:4808416/1‑100 (MQ=255)
                      tACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATg                                                                          >  1:2683814/1‑100 (MQ=255)
                       aCTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgg                                                                         <  2:2581037/100‑1 (MQ=255)
                       aCTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgg                                                                         >  2:3381299/1‑100 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgga                                                                        >  1:4852420/1‑100 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgga                                                                        >  2:783524/1‑100 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTCGGCAATCATgga                                                                        >  2:1400862/1‑100 (MQ=255)
                         tGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggag                                                                       <  1:4204426/100‑1 (MQ=255)
                          gAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggagg                                                                      >  2:2142406/1‑100 (MQ=255)
                            aCAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGaa                                                                    <  2:1820602/100‑1 (MQ=255)
                            aCAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGaa                                                                    <  1:585140/100‑1 (MQ=255)
                             cAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAg                                                                   >  1:3384530/1‑100 (MQ=255)
                              aGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGa                                                                  <  1:1027209/100‑1 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  1:1786017/1‑100 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  1:3254311/1‑100 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  1:1956098/1‑100 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  2:2729585/1‑100 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  2:446431/1‑100 (MQ=255)
                                aTGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATa                                                                <  1:4561683/100‑1 (MQ=255)
                                 tGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATaa                                                               >  2:2053181/1‑100 (MQ=255)
                                   cATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAgg                                                             >  1:104432/1‑100 (MQ=255)
                                   cATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAgg                                                             >  1:3237044/1‑100 (MQ=255)
                                    aTAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTTCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAACATAAGGt                                                            >  2:5106522/1‑100 (MQ=255)
                                    aTAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGt                                                            <  2:1197235/100‑1 (MQ=255)
                                    aTAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGt                                                            <  1:913168/100‑1 (MQ=255)
                                       aTGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCaa                                                         <  2:1590874/100‑1 (MQ=255)
                                         gCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGa                                                       >  1:1884046/1‑100 (MQ=255)
                                         gCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGGCGATTTCCCGCTCACCGTGAGGCATCGCATCCATTGGGCGATCATGGAGGAAGATAAGGTCAAGa                                                       >  1:5174382/1‑100 (MQ=255)
                                          cGTTGCGGCCGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGAt                                                      >  2:4530861/1‑100 (MQ=255)
                                          cGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGAt                                                      >  1:531058/1‑100 (MQ=255)
                                            ttGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCg                                                    <  2:3567294/100‑1 (MQ=255)
                                            ctGCGCGAGACGTACATAAACCTGCCTCCCATGGATTACCCGCCCACCGTCAGACATCGCATCCCTCGGGGAATCATGGAGGAAGATAAGGTCAGGATCg                                                    <  2:1082841/99‑1 (MQ=255)
                                             tGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGGCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCgg                                                   <  2:3402165/100‑1 (MQ=255)
                                              gcgcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGa                                                  >  1:3105910/1‑100 (MQ=255)
                                               cgcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGTCAATCATGGAGGAAGATAAGGTCAAGATCGGaa                                                 >  1:2211861/1‑100 (MQ=255)
                                               cgcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGaa                                                 >  1:2542561/1‑100 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  2:3353098/100‑1 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  1:1215417/100‑1 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  2:2629978/100‑1 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  2:4050237/100‑1 (MQ=255)
                                                  cAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTg                                              <  1:3923457/100‑1 (MQ=255)
                                                    gACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGCTCGGAACTGTa                                            >  2:3332434/1‑100 (MQ=255)
                                                    gACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTa                                            <  2:3139747/100‑1 (MQ=255)
                                                    gACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTa                                            >  1:217752/1‑100 (MQ=255)
                                                      cGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGc                                          <  2:3075250/100‑1 (MQ=255)
                                                       gCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGcc                                         <  2:3892974/100‑1 (MQ=255)
                                                       gCAAATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGcc                                         >  1:4294603/1‑100 (MQ=255)
                                                        cAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCt                                        <  2:2563416/100‑1 (MQ=255)
                                                          gATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTaa                                      >  2:4129441/1‑100 (MQ=255)
                                                           aTAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAAc                                     >  1:2941640/1‑100 (MQ=255)
                                                            tAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACt                                    >  1:2638853/1‑100 (MQ=255)
                                                            tAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACt                                    <  2:422118/100‑1 (MQ=255)
                                                            tAAAACTGCCGCACGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGCTAAGGTCAAGACCGGAACTGTAGCCTAACt                                    >  1:4802333/1‑100 (MQ=255)
                                                             aaaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTc                                   <  1:5097418/100‑1 (MQ=255)
                                                             aaaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTc                                   <  1:893808/100‑1 (MQ=255)
                                                              aaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTcc                                  >  2:1004277/1‑100 (MQ=255)
                                                              aaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTcc                                  >  1:1141419/1‑100 (MQ=255)
                                                               aaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCAACCATTGGGCAATCCTGGAGGCAGATAAGGTCAAGATCGGAACTGTAGCCTAACTccc                                 >  2:1792776/1‑100 (MQ=255)
                                                                 cTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAg                               <  1:551916/100‑1 (MQ=255)
                                                                  tGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgn                              <  1:5218412/100‑2 (MQ=255)
                                                                   gccgccCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgcc                             >  1:3819775/1‑100 (MQ=255)
                                                                   gccgccCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgcc                             >  1:4056454/1‑100 (MQ=255)
                                                                    ccgccCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgccg                            <  2:81819/100‑1 (MQ=255)
                                                                       cccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGccc                         <  1:986718/100‑1 (MQ=255)
                                                                       cccGTCGATTTCCCGCTCACCGTCAGGCATCGCATACATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGccc                         <  1:4798795/100‑1 (MQ=255)
                                                                        ccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCGATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCa                        <  1:2713964/100‑1 (MQ=255)
                                                                        ccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCa                        >  2:1475720/1‑100 (MQ=255)
                                                                        ccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCa                        >  2:4365769/1‑100 (MQ=255)
                                                                         gggcGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACGCCCAGCCGCCCAg                       <  2:1611820/97‑1 (MQ=255)
                                                                         cGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAg                       >  2:1136019/1‑100 (MQ=255)
                                                                         cGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAg                       <  1:4364328/100‑1 (MQ=255)
                                                                           tCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCt                     <  2:4816748/100‑1 (MQ=255)
                                                                              aTTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGc                  <  2:2177483/100‑1 (MQ=255)
                                                                              aTTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGc                  <  1:2308039/100‑1 (MQ=255)
                                                                              aTTTCCCGCTCACCGGCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGc                  <  1:1069897/100‑1 (MQ=255)
                                                                               tttCCGGCTCTCTGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGTCTAACTCCCAGCCGCCCAGCTTGcc                 >  2:2234357/1‑100 (MQ=255)
                                                                               tttCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGcc                 >  1:751132/1‑100 (MQ=255)
                                                                                  cccGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTa              >  1:3087545/1‑100 (MQ=255)
                                                                                    cccTCACGGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGCGCAAGATCGGAACTGTAGCCTAATTCCCAGCCCCCCAGCTTGCCGTAGc            <  2:120658/98‑1 (MQ=255)
                                                                                    cGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGc            >  2:5385173/1‑100 (MQ=255)
                                                                                       tCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGa         <  1:136777/100‑1 (MQ=255)
                                                                                          ccGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGacca      >  1:5522169/1‑100 (MQ=255)
                                                                                          ccGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGacca      >  1:4291173/1‑100 (MQ=255)
                                                                                            gtcaggcaTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGaccacc    >  1:3679458/1‑100 (MQ=255)
                                                                                            gtcaggcaTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGaccacc    >  1:1570627/1‑100 (MQ=255)
                                                                                            gtcaggcaTCGCATCCATTGGGCAAGCATGGAAGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGaccacc    >  1:538583/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAATGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGC  >  NC_000913/3197399‑3197592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑