Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,271,880 T→G E291A (GAA→GCA)  garR ← tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,8800TG98.2% 778.7 / ‑1.1 224E291A (GAA→GCA) garRtartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (105/115);  total (107/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 224 total aligned reads displayed.

TACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCG  >  NC_000913/3271783‑3271976
                                                                                                 |                                                                                                
tACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCg                                                                                                <  1:4692210/100‑1 (MQ=255)
 aCCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGa                                                                                               >  1:1426781/1‑100 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATTCGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                              <  2:4524727/100‑1 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACTCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                              <  2:5363130/100‑1 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                              <  1:239024/100‑1 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                              >  1:1877615/1‑100 (MQ=255)
    acaTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACtt                                                                                            >  1:3682875/1‑100 (MQ=255)
     caTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACttt                                                                                           <  2:4038223/100‑1 (MQ=255)
      aTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTc                                                                                          <  1:2986585/100‑1 (MQ=255)
      aTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTc                                                                                          >  1:1942172/1‑100 (MQ=255)
        gACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGc                                                                                        <  2:4727478/100‑1 (MQ=255)
          cGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCa                                                                                      >  2:2274406/1‑100 (MQ=255)
           gCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAg                                                                                     <  1:424066/100‑1 (MQ=255)
             cGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGtt                                                                                   <  1:3429894/100‑1 (MQ=255)
             cGGAGGGCGTTTCTCTTATTCGCCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAgat                                                                                   >  1:4664500/1‑98 (MQ=255)
              ggAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGttt                                                                                  <  1:3933603/100‑1 (MQ=255)
                 gggCGTTCCTATTATTCTGCCTGGCGTCACGGCCCGGATTGCGATACGCCATCGGGGAACGACGTAATTAACGAGTAACTTCGACTTTTGCCAGttttac                                                                               <  2:79769/100‑3 (MQ=255)
                 aggCGTTTCGCTTATTCGGCCTGGATTCCATGCACGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCCACTATCGCCGGTATTTc                                                                               >  2:4139088/2‑100 (MQ=255)
                  ggCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCTCCAGTTTTTCg                                                                              >  2:2236821/1‑100 (MQ=255)
                  ggCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCg                                                                              <  2:2168571/100‑1 (MQ=255)
                  ggCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCg                                                                              >  1:461432/1‑100 (MQ=255)
                    cGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgta                                                                            >  1:4340585/1‑100 (MQ=255)
                    cGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgta                                                                            >  1:3181869/1‑100 (MQ=255)
                     gTTTCTCTTATACGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGACACGACGTCATTAACTAGTAACTGCGACTTTCGCCATTTTTTCgtag                                                                           <  2:1272868/100‑1 (MQ=255)
                      tttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagt                                                                          <  1:4015296/100‑1 (MQ=255)
                      tttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagt                                                                          <  2:741212/100‑1 (MQ=255)
                      ttactctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagt                                                                          <  1:3372488/97‑1 (MQ=255)
                       ttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagta                                                                         <  1:5044383/100‑1 (MQ=255)
                         ctctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagtaag                                                                       >  1:3083301/1‑98 (MQ=255)
                         ctctTATTCGGCCGGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGc                                                                       <  1:1368111/100‑1 (MQ=255)
                          tctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCa                                                                      >  2:2730840/1‑100 (MQ=255)
                           ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCAc                                                                     <  1:117933/100‑1 (MQ=255)
                           ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCAc                                                                     >  2:609078/1‑100 (MQ=255)
                            ttATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACg                                                                    <  1:1060969/100‑1 (MQ=255)
                             tATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGc                                                                   >  2:551067/1‑100 (MQ=255)
                             atccgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGc                                                                   >  1:5361988/6‑100 (MQ=255)
                             atccgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGc                                                                   >  1:4498483/6‑100 (MQ=255)
                              aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACGTTCGCCAGGTTGTCGTAGTAGCACGcc                                                                  >  2:4972830/1‑100 (MQ=255)
                               ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                 >  1:176444/1‑100 (MQ=255)
                                tCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                                <  1:1537135/100‑1 (MQ=255)
                                ngggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTCTTCGTAGTAGCACGCCAg                                                                >  2:5069710/3‑100 (MQ=255)
                                cgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                                >  1:3298226/3‑100 (MQ=255)
                                cgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                                >  2:1700253/3‑100 (MQ=255)
                                  ggCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                              <  2:1856743/100‑1 (MQ=255)
                                  ggCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                              <  1:1735530/100‑1 (MQ=255)
                                    ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg                                                            <  2:3819489/100‑1 (MQ=255)
                                        gATTCCAGGCCCGGATTGCAATACGCCATGCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTAt                                                        <  1:5084521/100‑1 (MQ=255)
                                         aTTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATg                                                       >  1:653454/1‑100 (MQ=255)
                                             cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATcc                                                   <  2:456377/100‑1 (MQ=255)
                                             cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATcc                                                   <  2:3015407/100‑1 (MQ=255)
                                             cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGCAGCACGCCAGGGCGCTATGATcc                                                   >  2:2466287/1‑100 (MQ=255)
                                               ggCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgc                                                 <  1:5148699/100‑1 (MQ=255)
                                               ggCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgc                                                 >  1:1259783/1‑100 (MQ=255)
                                                 cccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgccg                                               >  1:210146/1‑100 (MQ=255)
                                                  ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTCTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt                                              >  1:2942621/1‑100 (MQ=255)
                                                  ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGTGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt                                              <  2:4975414/100‑1 (MQ=255)
                                                  ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt                                              <  1:2574039/100‑1 (MQ=255)
                                                    ggATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTc                                            <  1:2176496/100‑1 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                           >  1:1655097/1‑100 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                           <  1:159009/100‑1 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                           <  1:3643697/100‑1 (MQ=255)
                                                           aaTACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAcc                                     <  1:2556701/100‑1 (MQ=255)
                                                            aTACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa                                    >  1:2467884/1‑100 (MQ=255)
                                                              aCGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATc                                  >  2:3264527/1‑100 (MQ=255)
                                                              aCGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATc                                  <  2:5299667/100‑1 (MQ=255)
                                                               cGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCt                                 <  2:1507829/100‑1 (MQ=255)
                                                               cGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCt                                 <  1:5495860/100‑1 (MQ=255)
                                                                 ccATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGc                               >  1:3239105/1‑100 (MQ=255)
                                                                   aTCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTc                             <  1:3710070/100‑1 (MQ=255)
                                                                   aTCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTc                             >  2:5222141/1‑100 (MQ=255)
                                                                      cGGGCACTACGTCATTAACGAGTAACTGCGACTGTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                          <  1:2260024/100‑1 (MQ=255)
                                                                       gggCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAg                         <  2:2954357/100‑1 (MQ=255)
                                                                        ggCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGt                        <  1:5562102/100‑1 (MQ=255)
                                                                           acgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                     <  1:4101845/100‑1 (MQ=255)
                                                                           acgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                     <  1:4800113/100‑1 (MQ=255)
                                                                            cgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                    >  2:207016/1‑100 (MQ=255)
                                                                             gacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTg                   <  2:138403/100‑1 (MQ=255)
                                                                               cgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGca                 <  2:1115936/100‑1 (MQ=255)
                                                                                gTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat                <  1:2262698/100‑1 (MQ=255)
                                                                                gTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat                >  1:287287/1‑100 (MQ=255)
                                                                                 tCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc               >  2:3512627/1‑100 (MQ=255)
                                                                                 tCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc               <  1:1803553/100‑1 (MQ=255)
                                                                                 tCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc               >  1:2716217/1‑100 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca              >  1:4484471/1‑100 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca              >  1:354498/1‑100 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca              <  1:4040627/100‑1 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca              <  2:1008191/100‑1 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTAGGATCCGCCGTTCCTAAACCAGCCGCTCGCAGTGCCTGcatca              <  1:4747619/100‑1 (MQ=255)
                                                                                   aTTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat             >  2:3920697/1‑100 (MQ=255)
                                                                                   aTTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGACAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat             >  2:2574423/1‑100 (MQ=255)
                                                                                    ttAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCatcatc            <  2:2680869/100‑1 (MQ=255)
                                                                                    ttAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCatcatc            >  1:1560289/1‑100 (MQ=255)
                                                                                     tAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCTGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtct           <  2:3140093/100‑1 (MQ=255)
                                                                                      aaCGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc          >  1:5186366/1‑100 (MQ=255)
                                                                                       aCGAGTAACTGCGAGTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCTCAGTGCCTGCATCATCTcc         <  2:5027007/100‑1 (MQ=255)
                                                                                        cGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCa        <  2:5115268/100‑1 (MQ=255)
                                                                                        cGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCa        >  1:1985745/1‑100 (MQ=255)
                                                                                           gTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCGGCATAATCTCCATaa     >  2:4731574/1‑100 (MQ=255)
                                                                                            tAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAAc    >  1:3369471/1‑100 (MQ=255)
                                                                                             aaCTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAAcc   >  1:925708/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCG  >  NC_000913/3271783‑3271976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: