Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 400,871 T→G E18A (GAA→GCA)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,8710TG99.6% 849.4 / ‑4.6 241E18A (GAA→GCA) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (111/129);  total (112/129)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 241 total aligned reads displayed.

GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967
                                                                                                  |                                                                                                
gTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCa                                                                                                <  1:450614/100‑1 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               >  1:3120679/1‑100 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               <  1:2742537/100‑1 (MQ=255)
    cATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGtt                                                                                            >  1:3599321/1‑100 (MQ=255)
    cATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGtt                                                                                            >  1:2015913/1‑100 (MQ=255)
      ttGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTcc                                                                                          <  1:3483340/100‑1 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        <  1:113650/100‑1 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        >  2:4786169/1‑100 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        >  2:3042733/1‑100 (MQ=255)
        gCCCTTGTCTATCAATGCCCAGCAGCACAACGGCGAAGCGACTTTTATCAATGGCATCGACAATGTTTGTTGCGGATTGCAGAGTTACTGCATGTTCCGc                                                                                        >  2:4508980/1‑100 (MQ=255)
         cccTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCt                                                                                       >  1:580726/1‑100 (MQ=255)
           cTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGa                                                                                     >  2:5429786/1‑100 (MQ=255)
           cTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGa                                                                                     >  1:3704799/1‑100 (MQ=255)
             tGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtt                                                                                   >  2:941231/1‑100 (MQ=255)
             tGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtn                                                                                   <  1:4422681/100‑2 (MQ=255)
              gTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAttt                                                                                  >  1:392969/1‑100 (MQ=255)
               tttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTa                                                                                 >  1:4172835/1‑100 (MQ=255)
               cttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtttn                                                                                 <  2:5496081/99‑2 (MQ=255)
                ttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTac                                                                                >  2:10365/1‑100 (MQ=255)
                ttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTac                                                                                >  2:2602705/1‑100 (MQ=255)
                 tATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTacc                                                                               <  2:2006827/100‑1 (MQ=255)
                 tATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTacc                                                                               >  2:5039373/1‑100 (MQ=255)
                 naTCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaag                                                                               >  1:5570281/2‑98 (MQ=255)
                   tCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccac                                                                             >  2:217442/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            >  2:1539620/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            <  2:90630/100‑1 (MQ=255)
                     aaTGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTAccacca                                                                           <  2:1098689/100‑1 (MQ=255)
                        gCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaa                                                                        >  2:3465241/1‑100 (MQ=255)
                         cccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCTATTTCTGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                       >  2:1347333/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      >  2:2657869/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAACGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      <  1:2643493/100‑1 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGGTTTACCACCAAAAACg                                                                     >  2:442448/1‑100 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACg                                                                     <  2:2434146/100‑1 (MQ=255)
                             gcagcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAt                                                                   <  2:1936915/100‑1 (MQ=255)
                               agcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTc                                                                 <  2:3578264/100‑1 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                <  2:1813858/100‑1 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                <  1:2339969/100‑1 (MQ=255)
                                  acaACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTa                                                              <  1:1943039/100‑1 (MQ=255)
                                  acaACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTa                                                              <  1:4829539/100‑1 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             >  2:2770330/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             <  2:3351425/100‑1 (MQ=255)
                                    aaCGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGTAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                            <  1:1226415/100‑1 (MQ=255)
                                    aaCGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                            <  1:1018042/100‑1 (MQ=255)
                                      cGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCg                                                          <  1:2605079/100‑1 (MQ=255)
                                       gTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGc                                                         >  1:2912010/1‑100 (MQ=255)
                                         cGAAGCGACTTTTAGCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAg                                                       <  2:1478251/100‑1 (MQ=255)
                                               gACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGTATGATCCGCTGATTTACCACCAAAAACGATTTCTACCCGCAGTTTTTc                                                 <  1:4886763/100‑1 (MQ=255)
                                               gACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTc                                                 <  1:4718887/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTcc                                                <  2:1291463/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  2:3554076/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  1:5257525/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  2:3542529/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  1:2461606/1‑100 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  1:2536801/1‑100 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  2:1415155/1‑100 (MQ=255)
                                                   tttATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATc                                             <  1:2373686/100‑1 (MQ=255)
                                                    ttATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                            <  2:3233096/100‑1 (MQ=255)
                                                     tATCAATGGCATCGACAAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                            <  2:4620064/100‑1 (MQ=255)
                                                     tATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCtt                                           <  1:4930957/100‑1 (MQ=255)
                                                       tCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaa                                         <  2:3670701/100‑1 (MQ=255)
                                                       tCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaa                                         <  1:2753764/100‑1 (MQ=255)
                                                         aaTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaga                                       >  2:5353477/1‑98 (MQ=255)
                                                         aaTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaa                                       >  1:5454658/1‑100 (MQ=255)
                                                         aaTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaa                                       >  2:3149410/1‑100 (MQ=255)
                                                         aaTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGATTTTCCATCTTaaaa                                       >  1:698739/1‑100 (MQ=255)
                                                          aTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaaa                                      >  2:4283166/1‑100 (MQ=255)
                                                           tGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAc                                     <  1:4205141/100‑1 (MQ=255)
                                                            ggCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  1:678232/100‑1 (MQ=255)
                                                            ggCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    >  2:2300782/1‑100 (MQ=255)
                                                            ggCATCGACAATGTTTTTTGCCGATTGCAGAGACACGGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  1:3386538/100‑1 (MQ=255)
                                                              cATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTa                                  >  1:3435586/1‑100 (MQ=255)
                                                               aTCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTAt                                 <  1:120102/100‑1 (MQ=255)
                                                                 cGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               <  1:4107430/100‑1 (MQ=255)
                                                                 cGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               <  1:404707/100‑1 (MQ=255)
                                                                  gACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATccc                              >  1:271366/1‑100 (MQ=255)
                                                                   aCAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCg                             <  1:1407334/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  2:2163990/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  1:1775811/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  2:3783812/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  1:2428526/100‑1 (MQ=255)
                                                                        tGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTa                         <  1:3060650/100‑1 (MQ=255)
                                                                          ttttttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAac                       <  2:2054434/100‑1 (MQ=255)
                                                                               tGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACaaa                  >  1:508789/1‑100 (MQ=255)
                                                                                tctgATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAg                 <  2:3963599/97‑1 (MQ=255)
                                                                                gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTGCTACCCGCAGTTTTTCCCTCTTAAAAACCTATCCCATCTAACACAAAg                 >  1:5303330/1‑100 (MQ=255)
                                                                                gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAg                 <  2:623724/100‑1 (MQ=255)
                                                                                 ccGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGt                <  1:4002513/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  1:3654470/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  2:4243511/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  1:3475737/100‑1 (MQ=255)
                                                                                      tGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATa           <  1:2797142/100‑1 (MQ=255)
                                                                                      tGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATa           <  2:2548869/100‑1 (MQ=255)
                                                                                       ncAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTNCCATCTCAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  1:103988/2‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTACAAACCTATCCCGTCTAACACAAAGTGCATAc          >  1:1585139/1‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  2:2902765/1‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  2:122034/1‑100 (MQ=255)
                                                                                        cAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACa         >  1:3312173/1‑100 (MQ=255)
                                                                                          gagaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACAtt       >  2:692521/1‑100 (MQ=255)
                                                                                           agaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTa      <  1:3280602/100‑1 (MQ=255)
                                                                                             acacTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc    <  1:3021437/100‑1 (MQ=255)
                                                                                                  |                                                                                                
GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: