Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,290,794 A→C I661L (ATC→CTC)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,290,7940AC98.5% 734.2 / ‑2.0 208I661L (ATC→CTC) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (100/102);  total (101/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATC  >  NC_000913/3290697‑3290890
                                                                                                 |                                                                                                
cGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTAGTtctc                                                                                                <  1:4640823/100‑1 (MQ=255)
  gcAGTGACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGCTACCGGCGGCGTGGTTctcca                                                                                              <  1:4759679/99‑1 (MQ=255)
  ccAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcca                                                                                              <  1:3300501/100‑1 (MQ=255)
    aGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatc                                                                                            >  2:4367664/1‑100 (MQ=255)
    aGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatc                                                                                            <  1:173246/100‑1 (MQ=255)
    aGTTACCGTTAACCTTATGGCACCTTCAGTGCCGCATACAGCTACGGTAATGAAAGCAGCCAATACAATTACGGCGTTACCGGCGGGGTGGTtctcgcta                                                                                            >  2:5071113/1‑96 (MQ=255)
     gTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatcc                                                                                           <  2:2190112/100‑1 (MQ=255)
        aCCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCa                                                                                        <  2:545609/100‑1 (MQ=255)
         ccGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCAt                                                                                       >  2:1166673/1‑100 (MQ=255)
          cGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATGACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATg                                                                                      >  1:408542/1‑100 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgn                                                                                     <  2:204808/100‑2 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                     <  2:4002788/100‑1 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                     >  1:532105/1‑100 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                     >  2:4205999/1‑100 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                     <  2:4751435/100‑1 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                     >  1:3609032/1‑100 (MQ=255)
             tCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCg                                                                                   <  2:2947199/100‑1 (MQ=255)
             tCACCTTATGGCACCATCAGTGCCGTATACAGCTACGTTAATGACAGCAGCCAATACAAGTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCg                                                                                   <  2:1613172/100‑1 (MQ=255)
               aCCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTg                                                                                 >  1:4863530/1‑100 (MQ=255)
               aCCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTg                                                                                 <  1:1182539/100‑1 (MQ=255)
                ccTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                                >  1:2120161/1‑100 (MQ=255)
                ccTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                                <  1:2843911/100‑1 (MQ=255)
                 cTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGan                                                                               <  1:2393716/100‑2 (MQ=255)
                 cTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGAc                                                                               <  2:5222993/100‑1 (MQ=255)
                 cTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGAc                                                                               >  1:4285724/1‑100 (MQ=255)
                  ttATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACg                                                                              >  1:2978696/1‑100 (MQ=255)
                  ctATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACg                                                                              <  2:4393546/99‑1 (MQ=255)
                    ntGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACgag                                                                            >  1:4435463/2‑98 (MQ=255)
                     tGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGctc                                                                           <  1:1530487/100‑1 (MQ=255)
                      ggCAGCTTCCGTGCTCGATAGAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCAGCCTCATGGCGTGACGctct                                                                          <  2:146858/100‑1 (MQ=255)
                      ggCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGctct                                                                          >  1:922371/1‑100 (MQ=255)
                      ggCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGctct                                                                          >  2:2221775/1‑100 (MQ=255)
                       gCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCtctc                                                                         <  2:1749233/100‑1 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                        <  1:3584083/100‑1 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                        >  2:1082732/1‑100 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                        <  1:5046478/100‑1 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                        >  1:3859786/1‑100 (MQ=255)
                          ccTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCa                                                                      <  1:3805865/100‑1 (MQ=255)
                            ttCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAat                                                                    <  1:4121608/100‑1 (MQ=255)
                             tCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGCGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAata                                                                   <  2:2418881/100‑1 (MQ=255)
                                 tGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTg                                                               <  1:4686397/100‑1 (MQ=255)
                                  gCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTgg                                                              >  1:2416969/1‑100 (MQ=255)
                                   ccGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTggg                                                             >  2:4928282/1‑100 (MQ=255)
                                   ccGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTggg                                                             <  1:2901667/100‑1 (MQ=255)
                                   ccGGATACAGCGACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTggg                                                             <  2:3641259/100‑1 (MQ=255)
                                     ggATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCa                                                           >  2:1853212/1‑100 (MQ=255)
                                     ggATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCa                                                           <  2:1257098/100‑1 (MQ=255)
                                     gcatccAGCTACGGTAATGACAGCAGCCCATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCa                                                           <  2:4664212/95‑1 (MQ=255)
                                      gATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCaa                                                          >  1:786792/1‑100 (MQ=255)
                                      gATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCaa                                                          >  2:4692367/1‑100 (MQ=255)
                                       aTACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAAc                                                         <  1:5243148/100‑1 (MQ=255)
                                       aTACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAAc                                                         >  2:5373098/1‑100 (MQ=255)
                                            gCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCttt                                                    >  1:4605526/1‑100 (MQ=255)
                                            gCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCttt                                                    <  2:645356/100‑1 (MQ=255)
                                            gCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCttt                                                    <  2:1850513/100‑1 (MQ=255)
                                              tACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTg                                                  <  1:2064493/100‑1 (MQ=255)
                                               aCGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTgc                                                 >  1:1804979/1‑100 (MQ=255)
                                                  gTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCt                                              <  2:776653/100‑1 (MQ=255)
                                                  gTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCt                                              <  1:627696/100‑1 (MQ=255)
                                                   tAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCtt                                             <  2:4794908/100‑1 (MQ=255)
                                                     aTGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTAt                                           >  2:2093682/1‑100 (MQ=255)
                                                      tGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATc                                          >  1:3529108/1‑100 (MQ=255)
                                                      tGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATc                                          >  1:415948/1‑100 (MQ=255)
                                                       gACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCg                                         <  2:2714054/100‑1 (MQ=255)
                                                        aCAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGa                                        <  2:4299952/100‑1 (MQ=255)
                                                          agcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATg                                      <  2:2912441/100‑1 (MQ=255)
                                                          agcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATg                                      >  2:2830664/1‑100 (MQ=255)
                                                              gcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTaa                                  >  1:1860827/1‑100 (MQ=255)
                                                                 atctcAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                               <  1:69497/96‑1 (MQ=255)
                                                                 aaTACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                               <  2:1732029/100‑1 (MQ=255)
                                                                 aaTACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                               <  1:4722508/100‑1 (MQ=255)
                                                                 aaTACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                               <  2:1840047/100‑1 (MQ=255)
                                                                  aTACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGa                              >  1:5159653/1‑100 (MQ=255)
                                                                   tACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAg                             <  2:3175146/100‑1 (MQ=255)
                                                                     cAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCa                           >  2:4039585/1‑100 (MQ=255)
                                                                     cAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCa                           >  2:2995040/1‑100 (MQ=255)
                                                                      aaTTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCAGGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCan                          <  1:1597471/100‑2 (MQ=255)
                                                                       aTTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATc                         >  2:2555838/1‑100 (MQ=255)
                                                                       aTTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATc                         <  1:214181/100‑1 (MQ=255)
                                                                        ttACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCt                        >  1:5138039/1‑100 (MQ=255)
                                                                          aCGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTgg                      >  1:3962082/1‑100 (MQ=255)
                                                                           cGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGc                     >  1:2405072/1‑100 (MQ=255)
                                                                            ggCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCg                    <  2:2638027/100‑1 (MQ=255)
                                                                              cGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTg                  >  2:73827/1‑100 (MQ=255)
                                                                                ttACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAg                >  2:1140659/1‑100 (MQ=255)
                                                                                  aCCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGa              <  2:3553068/100‑1 (MQ=255)
                                                                                   ccGGCGGCGTGGTTCTCCATCCTCATGGCGTGATGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGAt             >  2:3971466/1‑100 (MQ=255)
                                                                                   ccGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGAt             <  1:2669136/100‑1 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa            >  1:5442966/1‑100 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa            <  2:698312/100‑1 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa            >  1:2880448/1‑100 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa            <  2:3833041/100‑1 (MQ=255)
                                                                                      gcggcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACa          <  1:139253/100‑1 (MQ=255)
                                                                                        ggcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaaa        >  1:1010970/1‑100 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc      <  2:1687276/100‑1 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc      <  2:2454290/100‑1 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc      <  2:349963/100‑1 (MQ=255)
                                                                                            tGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTa    >  2:5141802/1‑100 (MQ=255)
                                                                                            tGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTa    >  1:3706622/1‑100 (MQ=255)
                                                                                              gTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTAta  >  2:4502575/1‑99 (MQ=255)
                                                                                                 |                                                                                                
CGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATC  >  NC_000913/3290697‑3290890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: