Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,437,278 A→C D254A (GAT→GCT)  trkA → NAD‑binding component of TrK potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,2780AC99.5% 730.1 / NA 205D254A (GAT→GCT) trkANAD‑binding component of TrK potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (99/104);  minor base T (0/1);  total (100/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGA  >  NC_000913/3437182‑3437374
                                                                                                |                                                                                                
tgaGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTa                                                                                               <  1:1080170/100‑1 (MQ=255)
 gaGTGAATTACAGCGACTGGAAAAACCGTATAAGTGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTAc                                                                                              <  1:4743716/100‑1 (MQ=255)
  aGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACa                                                                                             <  2:2548491/100‑1 (MQ=255)
      aaTTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGt                                                                                         >  1:3283667/1‑100 (MQ=255)
       aTTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGtt                                                                                        >  1:3779389/1‑100 (MQ=255)
        ttACAGCGACTGGAAAAACCGTATAAGCGGAGCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTa                                                                                       <  1:265692/100‑1 (MQ=255)
         tACAGCGACTGGAAAAACCGTATAAGCGGATTATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTaa                                                                                      <  2:3898958/100‑1 (MQ=255)
         tACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTaa                                                                                      >  1:3418925/1‑100 (MQ=255)
          aCAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTaaa                                                                                     >  2:4871094/1‑100 (MQ=255)
          aCAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTaaa                                                                                     >  1:3859537/1‑100 (MQ=255)
          aCAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTaaa                                                                                     >  2:4417866/1‑100 (MQ=255)
           cagaaaCTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    <  2:349152/95‑1 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGGGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    <  2:4952755/100‑1 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    >  2:3040250/1‑100 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    <  1:3657268/100‑1 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    >  1:4261256/1‑100 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    >  2:2239076/1‑100 (MQ=255)
           cAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAAc                                                                                    >  2:922439/1‑100 (MQ=255)
                aCTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTAGTCTGGAAAAAGCTTACAGCGTTAAACTCATc                                                                               <  1:5105677/100‑1 (MQ=255)
                 cTGGACAAACCGTAAAGGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCg                                                                              <  1:1644294/100‑1 (MQ=255)
                  tGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGa                                                                             >  2:2487956/1‑100 (MQ=255)
                   ggAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGaa                                                                            >  1:1321660/1‑100 (MQ=255)
                   ggAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGaa                                                                            >  2:3552112/1‑100 (MQ=255)
                    gAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAAc                                                                           >  2:3983945/1‑100 (MQ=255)
                          ccGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATc                                                                     >  1:2676867/1‑100 (MQ=255)
                           cGTATAAGCGGATCATGCTGGTTGGTGGCGATAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATca                                                                    <  2:1730069/100‑1 (MQ=255)
                               taAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATcagcag                                                                >  1:3165529/1‑100 (MQ=255)
                                aaGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCagcagc                                                               >  2:3617785/1‑100 (MQ=255)
                                aaGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCagcagc                                                               >  1:1997698/1‑100 (MQ=255)
                                 aGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAgcg                                                              <  1:869961/100‑1 (MQ=255)
                                  gCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCTGCAgcgc                                                             >  2:2703731/1‑100 (MQ=255)
                                     gATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCt                                                          >  1:4626958/1‑100 (MQ=255)
                                      aTCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTg                                                         >  2:3175102/1‑100 (MQ=255)
                                      aTCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTg                                                         <  1:3728003/100‑1 (MQ=255)
                                        cATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGcc                                                       <  2:1687762/100‑1 (MQ=255)
                                         aTGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCg                                                      <  2:3809959/100‑1 (MQ=255)
                                         aTGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCg                                                      >  1:2215767/1‑100 (MQ=255)
                                           gCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGTGCGCTGCCGaa                                                    >  2:4177015/1‑100 (MQ=255)
                                           gCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGaa                                                    <  1:4861503/100‑1 (MQ=255)
                                             tggttggtGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACt                                                  >  1:5466882/1‑100 (MQ=255)
                                               gttggtGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTgg                                                <  2:2064352/100‑1 (MQ=255)
                                                ttggtGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGc                                               >  2:4159120/1‑100 (MQ=255)
                                                ttggtGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGc                                               <  1:1553311/100‑1 (MQ=255)
                                                ttggtGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGc                                               <  2:3594874/100‑1 (MQ=255)
                                                 tggtggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCg                                              <  1:3068843/100‑1 (MQ=255)
                                                 tggtggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCg                                              <  1:2975141/100‑1 (MQ=255)
                                                  ggtggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCgg                                             >  2:1302847/1‑100 (MQ=255)
                                                     ggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGaaa                                          >  1:4102009/1‑100 (MQ=255)
                                                     ggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGaaa                                          >  1:3543012/1‑100 (MQ=255)
                                                     ggCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGaaa                                          >  1:800108/1‑100 (MQ=255)
                                                      gCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGaaaa                                         <  2:5169/100‑1 (MQ=255)
                                                      gCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGaaaa                                         <  2:819842/100‑1 (MQ=255)
                                                       cGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAg                                        >  1:2511832/1‑100 (MQ=255)
                                                       cGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAg                                        >  1:578825/1‑100 (MQ=255)
                                                        ggTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGt                                       <  1:4145106/100‑1 (MQ=255)
                                                        ggTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGt                                       >  2:1058531/1‑100 (MQ=255)
                                                         gtagtaTCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGtt                                      >  2:5043738/1‑100 (MQ=255)
                                                          tAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                     <  2:2609381/100‑1 (MQ=255)
                                                           aaTATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTAc                                    <  1:4393133/100‑1 (MQ=255)
                                                              atCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTTCAGa                                 <  2:4673690/100‑1 (MQ=255)
                                                              atCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGa                                 >  1:4213256/1‑100 (MQ=255)
                                                                cGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAAt                               >  2:440026/1‑100 (MQ=255)
                                                                 ggTGCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATa                              <  1:236072/100‑1 (MQ=255)
                                                                  gTGGAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATAc                             <  2:4706766/100‑1 (MQ=255)
                                                                    gCAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGa                           >  1:2552094/1‑100 (MQ=255)
                                                                     cAGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACGCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGAt                          <  2:5494191/100‑1 (MQ=255)
                                                                      aggattGGCGCGTCGACTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGGAATCAGCAGCGCGCTGCCGAACTGTCGGAAAAGTTACATAATTCGATc                         <  2:3305433/95‑1 (MQ=255)
                                                                      aGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATc                         <  2:4350796/100‑1 (MQ=255)
                                                                      aGGGCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATc                         >  2:5296224/1‑100 (MQ=255)
                                                                       gggCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCg                        >  1:1850959/1‑100 (MQ=255)
                                                                       gggCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCg                        <  2:582839/100‑1 (MQ=255)
                                                                       gggCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCg                        >  1:3955373/1‑100 (MQ=255)
                                                                         gCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTc                      >  2:1182764/1‑100 (MQ=255)
                                                                         gCTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTc                      <  1:3567443/100‑1 (MQ=255)
                                                                          cTGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCt                     >  1:5214712/1‑100 (MQ=255)
                                                                           tGGCGCGTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCtt                    >  1:2364815/1‑100 (MQ=255)
                                                                              cgcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGGAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCttttt                 >  1:5022930/1‑100 (MQ=255)
                                                                              cgcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCttttt                 <  1:2461577/100‑1 (MQ=255)
                                                                              cgcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCttttt                 <  1:1252064/100‑1 (MQ=255)
                                                                              cgcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCttttt                 >  1:2071622/1‑100 (MQ=255)
                                                                               gcgTCGTCTGGCAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCtttttt                <  2:4388158/100‑1 (MQ=255)
                                                                               gcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCtttttt                <  1:3699400/100‑1 (MQ=255)
                                                                               gcgTCGTCTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCtttttt                >  2:2892732/1‑100 (MQ=255)
                                                                                 gtcgtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTgg              <  1:2864710/100‑1 (MQ=255)
                                                                                 gtcgtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTgg              <  1:1893791/100‑1 (MQ=255)
                                                                                   cgtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTg            <  1:934752/100‑1 (MQ=255)
                                                                                    gtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAACAGTTACAGCATACGATCGTCTTTTTTGGTGa           >  2:1989679/1‑100 (MQ=255)
                                                                                    gtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGa           >  1:3244222/1‑100 (MQ=255)
                                                                                    gtcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGa           >  2:1888930/1‑100 (MQ=255)
                                                                                     tcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGAt          >  1:2201022/1‑100 (MQ=255)
                                                                                     tcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGAt          <  1:4233032/100‑1 (MQ=255)
                                                                                     tcTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGAt          >  1:3604301/1‑100 (MQ=255)
                                                                                     aaagaaaaaaGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGAt          <  2:4428269/95‑1 (MQ=255)
                                                                                      cTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATg         >  2:637017/1‑100 (MQ=255)
                                                                                      cTGGAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATg         <  1:5517639/100‑1 (MQ=255)
                                                                                        ggAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCg       <  1:1200259/100‑1 (MQ=255)
                                                                                        ggAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCg       >  1:2790885/1‑100 (MQ=255)
                                                                                         gAAAAAGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGt      >  2:4196464/1‑100 (MQ=255)
                                                                                           aaaaGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCg    <  2:264278/100‑1 (MQ=255)
                                                                                             aaGCTTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCgag  >  2:2791793/1‑98 (MQ=255)
                                                                                                |                                                                                                
TGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGA  >  NC_000913/3437182‑3437374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑