Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,473,6120AC98.7% 842.1 / ‑0.6 235L157* (TTA→TGA) rpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (121/111);  total (122/113)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 235 total aligned reads displayed.

TTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAG  >  NC_000913/3473515‑3473708
                                                                                                 |                                                                                                
 tGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCAcc                                                                                               >  1:2821708/1‑100 (MQ=255)
  gTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCa                                                                                              >  1:5245085/1‑100 (MQ=255)
  gTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCa                                                                                              >  1:1003493/1‑100 (MQ=255)
   tttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCaa                                                                                             >  1:1114164/1‑100 (MQ=255)
    ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAAc                                                                                            >  1:1870727/1‑100 (MQ=255)
     tCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTTACTAAAACTCCGAAGGGATCACCAACg                                                                                           >  2:1200395/1‑100 (MQ=255)
     tCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACg                                                                                           <  1:2812349/100‑1 (MQ=255)
       cTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGt                                                                                         >  1:5569471/1‑100 (MQ=255)
          gTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgt                                                                                      >  2:3331449/1‑100 (MQ=255)
           tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtg                                                                                     >  2:786426/1‑100 (MQ=255)
            ttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtgt                                                                                    >  2:1337199/1‑100 (MQ=255)
             tATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGtgtg                                                                                   <  2:3833378/100‑1 (MQ=255)
              aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGc                                                                                  <  2:1666805/100‑1 (MQ=255)
                   ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACg                                                                             <  1:3528762/100‑1 (MQ=255)
                    tAGGCGTTCAATTTAAGTAGCCCAAAGCGGGATGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGc                                                                            >  1:5361561/1‑100 (MQ=255)
                     aGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGcc                                                                           >  1:3869856/1‑100 (MQ=255)
                       gCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCtt                                                                         <  2:3492571/100‑1 (MQ=255)
                        cGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttg                                                                        >  1:619178/1‑100 (MQ=255)
                        cGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttg                                                                        >  1:2208756/1‑100 (MQ=255)
                         nttCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  1:1181516/2‑100 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  2:3203209/1‑100 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  2:5333978/1‑100 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGACTGCCTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  2:5004894/1‑100 (MQ=255)
                           tCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                     <  1:2448237/100‑1 (MQ=255)
                           tCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                     <  1:1696830/100‑1 (MQ=255)
                           tCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                     <  1:4009779/100‑1 (MQ=255)
                           ccAATCTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                     <  2:5121777/99‑1 (MQ=255)
                            cAATTTAAGTAGCCCAAAGCGTGCTTCTTACTGCATGCGCCCGCCTGGTGACTAAAGCTCCGAAGGTATCACCAACGGTAGTGTGCGAACGCCTTGTTgg                                                                    >  2:1502521/1‑100 (MQ=255)
                             aaTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGc                                                                   >  2:4472395/1‑100 (MQ=255)
                             aaTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGc                                                                   <  1:963925/100‑1 (MQ=255)
                                  aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                              <  1:5100015/100‑1 (MQ=255)
                                  aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                              <  1:1332346/100‑1 (MQ=255)
                                   aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGc                                                             <  2:282516/100‑1 (MQ=255)
                                   aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGCTGGCTAAAACGCCGTAGGCACCACCAACGCGATTGGGCGAGCGCCTGGTTGTCTTcccc                                                             >  1:3057684/1‑97 (MQ=255)
                                    gTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            <  1:2183884/100‑1 (MQ=255)
                                    gTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            <  1:134779/100‑1 (MQ=255)
                                    gTAGCCCAAAGCGGGCCGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            <  1:4581886/100‑1 (MQ=255)
                                     tAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCa                                                           >  2:882935/1‑100 (MQ=255)
                                      aGCCCAAAGCTGGCTGCTTAGTTGAAGCGCCCGCCTAGTGACTAAAACTCCGAAGGCAGCACCAAGGCTAGTGTGCTAACGCCTTGTTGGCTTCGGCCac                                                          <  2:1960105/100‑2 (MQ=255)
                                       gCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATa                                                         >  2:1433534/1‑100 (MQ=255)
                                        cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATAc                                                        >  2:213458/1‑100 (MQ=255)
                                         ccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                       >  2:4110376/1‑100 (MQ=255)
                                         ccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                       >  1:746479/1‑100 (MQ=255)
                                           aaaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                     >  2:2554656/1‑100 (MQ=255)
                                           aaaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACAAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                     >  2:5369804/1‑100 (MQ=255)
                                            aaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                    >  2:1585436/1‑100 (MQ=255)
                                             aGCGTGCTGCTTACTGGGAGCGCCCGCCTTGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGa                                                   <  2:738671/100‑1 (MQ=255)
                                             aGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGa                                                   <  2:437094/100‑1 (MQ=255)
                                               cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                 >  2:648616/1‑100 (MQ=255)
                                               cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                 <  1:1559535/100‑1 (MQ=255)
                                               cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                 <  1:4735315/100‑1 (MQ=255)
                                                gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACg                                                <  2:574288/100‑1 (MQ=255)
                                                gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACg                                                <  1:1809376/100‑1 (MQ=255)
                                                 ggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGt                                               >  1:3595086/1‑100 (MQ=255)
                                                     gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTc                                           <  2:5173541/100‑1 (MQ=255)
                                                      ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                          <  2:2039609/100‑1 (MQ=255)
                                                      ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                          >  1:2331393/1‑100 (MQ=255)
                                                      ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                          <  1:58603/100‑1 (MQ=255)
                                                       ttACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCAc                                         <  1:3046191/100‑1 (MQ=255)
                                                         aCTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt                                       >  2:5151895/1‑100 (MQ=255)
                                                          cTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                      >  1:3330031/1‑100 (MQ=255)
                                                          cTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                      >  2:2276569/1‑100 (MQ=255)
                                                          cTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                      >  2:2717883/1‑100 (MQ=255)
                                                            ggAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTc                                    <  1:3682680/100‑1 (MQ=255)
                                                            ggAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTc                                    <  2:5443130/100‑1 (MQ=255)
                                                            ggAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTc                                    >  1:4500416/1‑100 (MQ=255)
                                                            ggAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTc                                    <  1:4844011/100‑1 (MQ=255)
                                                            ggAAGCGCCCGCCCGGTGACTAAAACTCGGAAGGGATCACCAACGGTAGTGTGCGAACGACTTGTTGGCTTCGGCCATACGGTGAACGGCTTCACGTTTc                                    >  1:4910332/1‑100 (MQ=255)
                                                             gAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCt                                   <  2:704960/100‑1 (MQ=255)
                                                               aGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTa                                 <  2:4437914/100‑1 (MQ=255)
                                                               aGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTa                                 >  2:2785776/1‑100 (MQ=255)
                                                               aGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTa                                 <  1:4305533/100‑1 (MQ=255)
                                                                  gcccgccTGGTGACTAAAACTCCGAAGGTATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                              >  1:4384808/1‑100 (MQ=255)
                                                                  gcccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                              <  2:1776006/100‑1 (MQ=255)
                                                                  gcccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                              <  1:5452075/100‑1 (MQ=255)
                                                                   cccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTg                             <  1:4406609/100‑1 (MQ=255)
                                                                     cgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa                           >  2:3094062/1‑100 (MQ=255)
                                                                        cTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTa                        <  1:2290417/100‑1 (MQ=255)
                                                                          ggTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAcc                      <  2:3730400/100‑1 (MQ=255)
                                                                           gTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCt                     >  2:4219636/1‑100 (MQ=255)
                                                                             gACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                   >  1:4163689/1‑100 (MQ=255)
                                                                              aCTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTg                  <  1:2077353/100‑1 (MQ=255)
                                                                              aCTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTg                  >  2:5455244/1‑100 (MQ=255)
                                                                              aCTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACAGTGAACGTCTTCACGGTTCTTAACTGCAGTACCTTTg                  >  1:5187386/1‑100 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTTCGAACGCCTTGTTGGCTCCGGCCATACGATGTACGTCTTCACGTTTCTTAACTGCCGTACCTTTGt                 >  2:4027555/1‑100 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                 <  2:3419436/100‑1 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                 >  1:2610105/1‑100 (MQ=255)
                                                                                 aaaaCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGttt               >  2:2810626/1‑100 (MQ=255)
                                                                                   aaCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTc             <  1:2056200/100‑1 (MQ=255)
                                                                                    aCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATATGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt            <  1:1460009/100‑1 (MQ=255)
                                                                                    aCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt            <  1:1492914/100‑1 (MQ=255)
                                                                                     cTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTg           >  2:2978660/1‑100 (MQ=255)
                                                                                      tCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACTTTTCTTAACTGCAGTACCTTTGTTTTCTgc          <  1:1592061/100‑1 (MQ=255)
                                                                                      tCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgc          <  2:1808306/100‑1 (MQ=255)
                                                                                       ccGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgca         <  2:2443820/100‑1 (MQ=255)
                                                                                       ccGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgca         <  1:5147063/100‑1 (MQ=255)
                                                                                         gAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagc       <  1:894748/100‑1 (MQ=255)
                                                                                            gggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc    <  1:2744164/100‑1 (MQ=255)
                                                                                             ggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCa   >  2:2301501/1‑100 (MQ=255)
                                                                                              gATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAg  <  2:2679504/100‑1 (MQ=255)
                                                                                                 |                                                                                                
TTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAG  >  NC_000913/3473515‑3473708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑