Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,479,233 A→C S459A (TCT→GCT)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,479,2330AC97.6% 727.2 / 3.9 212S459A (TCT→GCT) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base C (122/85);  total (123/89)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 218 total aligned reads displayed.

GCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGT  >  NC_000913/3479137‑3479329
                                                                                                |                                                                                                
gCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACg                                                                                               <  1:4202680/100‑1 (MQ=255)
  ggAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTa                                                                                             >  1:1507418/1‑100 (MQ=255)
       gTGCTGTTGGCATATTTCTACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGa                                                                                        >  2:1549067/1‑100 (MQ=255)
       gTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCCGCACGTAAAAGa                                                                                        >  2:4783936/1‑100 (MQ=255)
       gTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGa                                                                                        >  2:503864/1‑100 (MQ=255)
        tGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGAt                                                                                       >  1:1567288/1‑100 (MQ=255)
         gCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATc                                                                                      >  2:3069806/1‑100 (MQ=255)
          cTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCg                                                                                     <  2:3442216/100‑1 (MQ=255)
          cTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACCCAGCGGCCTCTGCACCCGCAGCTCGTAAAAGATCg                                                                                     >  1:1429370/1‑100 (MQ=255)
          cTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACCATATACTCAGCGGCCTCTGCACGCGCAGCACGTAAACGATCg                                                                                     >  2:1194744/1‑100 (MQ=255)
           tGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTACTGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGa                                                                                    >  2:4847892/1‑100 (MQ=255)
           tGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGa                                                                                    <  1:5159999/100‑1 (MQ=255)
           tGTTGGCACATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCCGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGa                                                                                    <  2:4417688/100‑1 (MQ=255)
             ttGGCATATTTCCGCCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGAcc                                                                                  <  2:3175972/100‑1 (MQ=255)
              tGGCATACTACCACTCGCTTCATGTTGTCTTGCGGCACGTTGCACGTAATGATCATAGAGTCAGCGGCCTCTTCACCCGCAGCACGTAAAAGATCGACCt                                                                                 >  2:603720/1‑100 (MQ=255)
                gCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGc                                                                               >  1:4306538/1‑100 (MQ=255)
                 cATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCg                                                                              <  2:2612321/100‑1 (MQ=255)
                 cATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCg                                                                              <  1:3678926/100‑1 (MQ=255)
                 cATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCg                                                                              >  1:4330464/1‑100 (MQ=255)
                   tatTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTg                                                                            >  1:5006095/1‑100 (MQ=255)
                    atTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTgg                                                                           >  1:2835493/1‑100 (MQ=255)
                    atTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTgg                                                                           >  2:157330/1‑100 (MQ=255)
                    atTTCCACAAACTTCATGGTGCATTCCGCCTCATTACAGGTAATAACGAGACACTCAGCGGTCACTGCACAACCAGCACGTAAAAGATCGACCTGCGTgg                                                                           <  2:1237579/100‑1 (MQ=255)
                       tCCGCCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGt                                                                        >  1:4282572/1‑100 (MQ=255)
                       tCCACCAGCATCATGGTGTCTTCTGGCTCGTTACAGGTAATGCCGATAGACTCAGCGGCCGCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGt                                                                        <  1:4852456/100‑1 (MQ=255)
                        ccaccaGCTTCATGGTGTCTTCCGGCTCTTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAAATCGACCTGCGTGGCGTc                                                                       <  2:842307/100‑1 (MQ=255)
                          accaGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGc                                                                     >  1:5467624/1‑100 (MQ=255)
                           ccaGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGcc                                                                    >  1:2919887/1‑100 (MQ=255)
                            caGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCg                                                                   <  2:1284882/100‑1 (MQ=255)
                                ttCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGttaa                                                               >  2:1406819/1‑97 (MQ=255)
                                 tCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGtaata                                                              >  2:92559/1‑100 (MQ=255)
                                    tGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAAc                                                           >  1:1761438/1‑100 (MQ=255)
                                    tGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAAc                                                           >  2:2143225/1‑100 (MQ=255)
                                     ggTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGTACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACt                                                          >  2:4256785/1‑100 (MQ=255)
                                       tgtCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACttt                                                        <  1:4595088/100‑1 (MQ=255)
                                        gtCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTg                                                       <  2:2096871/100‑1 (MQ=255)
                                        gtCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTg                                                       <  2:2892355/100‑1 (MQ=255)
                                         tCTTCCGGCTCGTTACAGGTAGTGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGt                                                      >  1:2647864/1‑100 (MQ=255)
                                         tCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGt                                                      >  1:1033531/1‑100 (MQ=255)
                                          cTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTa                                                     >  1:1823829/1‑100 (MQ=255)
                                           ttCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAg                                                    >  1:2499941/1‑100 (MQ=255)
                                           ttCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAg                                                    <  1:1672627/100‑1 (MQ=255)
                                           ttCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAg                                                    <  2:3764160/100‑1 (MQ=255)
                                            tCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGc                                                   >  2:1716766/1‑100 (MQ=255)
                                            tCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGc                                                   <  2:1001641/100‑1 (MQ=255)
                                             ccGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGcc                                                  <  1:1913601/100‑1 (MQ=255)
                                               ggCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGt                                                >  1:4410119/1‑100 (MQ=255)
                                               ggCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGt                                                >  1:3735755/1‑100 (MQ=255)
                                                gCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTa                                               <  2:3979664/100‑1 (MQ=255)
                                                gCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTa                                               <  2:1488314/100‑1 (MQ=255)
                                                  tCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTAtt                                             <  1:4042653/100‑1 (MQ=255)
                                                   cGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTAttt                                            >  1:112097/1‑100 (MQ=255)
                                                    gTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTa                                           >  2:2290236/1‑100 (MQ=255)
                                                     ttACAGGTAATGACGATAGACTCAGAGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTAc                                          >  2:4382670/1‑100 (MQ=255)
                                                      tACAGGTAATGACGATGGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACg                                         <  2:1860109/100‑1 (MQ=255)
                                                      tACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACg                                         <  1:520235/100‑1 (MQ=255)
                                                       caaGGTAAGGGCGATAGACTCAGCGGCCTCTGCACCCGCAGCACTTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGc                                        <  2:4647465/98‑1 (MQ=255)
                                                       aCAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGc                                        <  2:2350483/100‑1 (MQ=255)
                                                        cAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCa                                       <  2:2809631/100‑1 (MQ=255)
                                                          ggTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATc                                     >  1:3374248/1‑100 (MQ=255)
                                                          ggTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATc                                     >  2:4060359/1‑100 (MQ=255)
                                                          ggTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATc                                     >  1:3598355/1‑100 (MQ=255)
                                                          ggTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATc                                     >  1:261880/1‑100 (MQ=255)
                                                            tAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAg                                   <  1:3526532/100‑1 (MQ=255)
                                                               tGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGtt                                <  1:711827/100‑1 (MQ=255)
                                                               tGACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGtt                                >  1:5550186/1‑100 (MQ=255)
                                                                gACGATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTa                               >  1:2209477/1‑100 (MQ=255)
                                                                   gATAGACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACg                            <  2:4936363/100‑1 (MQ=255)
                                                                       gACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                        >  1:1014705/1‑100 (MQ=255)
                                                                       gACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                        >  1:5024159/1‑100 (MQ=255)
                                                                       gACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                        >  1:5435173/1‑100 (MQ=255)
                                                                       gACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                        >  1:663240/1‑100 (MQ=255)
                                                                       gACTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                        <  1:4890532/100‑1 (MQ=255)
                                                                        aCTCAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCt                       <  2:3664017/100‑1 (MQ=255)
                                                                         cTCATCGGCCGCTGCACCCGCAGCACGTAAAAGATCGATCTGCGTGGCGTCGCCGTAGTAAACATTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTg                      <  2:4556695/100‑1 (MQ=255)
                                                                           cAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGat                    <  1:3066976/100‑1 (MQ=255)
                                                                           cAGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAAATCTGTAGCCGTAATTGCGCATCAGGTTAACGGCGCTGat                    >  2:4103923/1‑100 (MQ=255)
                                                                           cAGCGGCCTCTGCACCCGCAGCACGGAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTCTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGat                    >  2:2178465/1‑100 (MQ=255)
                                                                            aGCGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGata                   >  1:3181637/1‑100 (MQ=255)
                                                                              cGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATc                 >  1:1259460/1‑100 (MQ=255)
                                                                              cGGCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATc                 >  1:3017446/1‑100 (MQ=255)
                                                                                gCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATccc               >  1:2000395/1‑100 (MQ=255)
                                                                                gCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATccc               <  2:3210370/100‑1 (MQ=255)
                                                                                gCCTCTGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATccc               <  2:523981/100‑1 (MQ=255)
                                                                                 ccTCTGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCg              >  1:2368450/1‑100 (MQ=255)
                                                                                  ctctGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGc             <  2:2698641/100‑1 (MQ=255)
                                                                                   tctGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCt            >  1:3262204/1‑100 (MQ=255)
                                                                                   tctGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCt            <  1:3957803/100‑1 (MQ=255)
                                                                                    ctGCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTc           <  2:1915433/100‑1 (MQ=255)
                                                                                      gCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCa         >  1:241732/1‑100 (MQ=255)
                                                                                      gCACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCa         <  1:1656756/100‑1 (MQ=255)
                                                                                       cagccGCAGAACGTAAAAGATCGACCTGCGGGGAGTCGCTGTAATAAAATTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAg        <  2:2037901/97‑1 (MQ=255)
                                                                                       cACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAg        >  2:3338966/1‑100 (MQ=255)
                                                                                       cACCCGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAg        >  2:3522282/1‑100 (MQ=255)
                                                                                         cccGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCa      <  1:2772021/100‑1 (MQ=255)
                                                                                           cGCAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACg    <  2:1432194/100‑1 (MQ=255)
                                                                                            gcagcaTGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACgg   <  2:3001920/100‑1 (MQ=255)
                                                                                            gcagcaCGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACgg   <  1:4816018/100‑1 (MQ=255)
                                                                                             cAGCACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGt  <  2:358293/100‑1 (MQ=255)
                                                                                             cAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGt  <  1:1364473/100‑1 (MQ=255)
                                                                                                |                                                                                                
GCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGT  >  NC_000913/3479137‑3479329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: