Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,515,115 A→C L267V (TTG→GTG)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,515,1150AC99.0% 695.7 / ‑3.3 192L267V (TTG→GTG) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (95/95);  total (96/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 196 total aligned reads displayed.

TCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACTC  >  NC_000913/3515019‑3515211
                                                                                                |                                                                                                
tCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGc                                                                                               <  2:1927646/100‑1 (MQ=255)
tCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGc                                                                                               <  2:208649/100‑1 (MQ=255)
  aGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCca                                                                                             >  2:2253197/1‑100 (MQ=255)
   gCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcag                                                                                            <  2:4300010/100‑1 (MQ=255)
    ccGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAcagccagc                                                                                           <  1:3097407/100‑1 (MQ=255)
    ccGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAcagccagc                                                                                           <  1:2718127/100‑1 (MQ=255)
     cGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagca                                                                                          <  1:199986/100‑1 (MQ=255)
     cGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagca                                                                                          >  2:1001877/1‑100 (MQ=255)
      gACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagcan                                                                                         <  1:1860331/100‑2 (MQ=255)
      gACAGAATTGAGGAGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagcag                                                                                         <  1:4202450/100‑1 (MQ=255)
       aCAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGt                                                                                        <  2:4458723/100‑1 (MQ=255)
        cAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGtt                                                                                       <  1:1910978/100‑1 (MQ=255)
         aGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTc                                                                                      <  2:89104/100‑1 (MQ=255)
          gAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCg                                                                                     <  1:3473469/100‑1 (MQ=255)
          gAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCg                                                                                     >  1:4371313/1‑100 (MQ=255)
            aTTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTc                                                                                   >  2:3499352/1‑100 (MQ=255)
                aGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCAcc                                                                               >  1:321507/1‑100 (MQ=255)
                aGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCAcc                                                                               <  1:4584174/100‑1 (MQ=255)
                 gggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCt                                                                              >  1:1541815/1‑100 (MQ=255)
                 gggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCt                                                                              >  1:1424564/1‑100 (MQ=255)
                 gggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCt                                                                              >  1:4305350/1‑100 (MQ=255)
                      cAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTa                                                                         >  2:334825/1‑100 (MQ=255)
                       aaTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTAc                                                                        <  2:1172990/100‑1 (MQ=255)
                       aaTCAAATACTGATTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCtttttcc                                                                        >  1:4797835/1‑98 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       >  2:493490/1‑100 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       >  1:1073224/1‑100 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       >  1:2978847/1‑100 (MQ=255)
                          cAAATGCTGTTTCGTCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTGCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtg                                                                     <  2:1346058/100‑1 (MQ=255)
                          cAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtg                                                                     >  1:900290/1‑100 (MQ=255)
                          cAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtg                                                                     <  1:4919457/100‑1 (MQ=255)
                          cAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtg                                                                     <  2:4101942/100‑1 (MQ=255)
                           aaaTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtgt                                                                    >  1:2880461/1‑100 (MQ=255)
                            aaTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGtgtg                                                                   <  1:2292490/100‑1 (MQ=255)
                            aaTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGtgtg                                                                   >  2:722509/1‑100 (MQ=255)
                              tACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTgcc                                                                 >  1:3642931/1‑100 (MQ=255)
                              tACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTgcc                                                                 >  1:5092214/1‑100 (MQ=255)
                               aCTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTgccg                                                                >  2:128016/1‑100 (MQ=255)
                                  gTTTCATCCGCTTCTCCTTGAGAATTATTTTTTTTTTTTTTTTTCGACTCCTGGTTTGTACACAGCCATCAGTTTGTCCACCTTTTTACGTGTGccgccg                                                             >  2:5343101/1‑100 (MQ=255)
                                   tttCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGt                                                            <  1:3826670/100‑1 (MQ=255)
                                      cATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgc                                                         >  2:5128640/1‑100 (MQ=255)
                                       aTCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgct                                                        >  1:2040371/1‑100 (MQ=255)
                                        tCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgctg                                                       <  2:3718296/100‑1 (MQ=255)
                                         ccGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGGAACGACTCCTGGTTTGTAAACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgctgc                                                      <  2:1572089/100‑1 (MQ=255)
                                         ccGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgctgc                                                      >  1:2643180/1‑100 (MQ=255)
                                           gCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCtt                                                    >  1:3993877/1‑100 (MQ=255)
                                            cTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTta                                                   <  1:2373568/100‑1 (MQ=255)
                                            cTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACAACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTta                                                   <  1:1945383/100‑1 (MQ=255)
                                            cTTCTCCTTGAGAATTATTTTTTCGCGGGTAAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTta                                                   <  2:602673/100‑1 (MQ=255)
                                               ctcCTTGAGAATTATTTTTTCGCGGATGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATAc                                                <  2:5389170/100‑1 (MQ=255)
                                                tcCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACt                                               >  2:3120020/1‑100 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              <  1:252766/100‑1 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              <  1:3163834/100‑1 (MQ=255)
                                                  cTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGc                                             >  1:100790/1‑100 (MQ=255)
                                                   ttGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCg                                            <  1:5343907/100‑1 (MQ=255)
                                                    tGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGt                                           >  1:3576822/1‑100 (MQ=255)
                                                    tGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGt                                           >  2:1743493/1‑100 (MQ=255)
                                                      tgaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCg                                         <  2:1386493/99‑1 (MQ=255)
                                                       gaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGa                                        >  2:4418803/1‑100 (MQ=255)
                                                       gaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGa                                        >  2:3295071/1‑100 (MQ=255)
                                                       gaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTCTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGa                                        >  2:5152703/1‑100 (MQ=255)
                                                           tattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACtt                                    <  1:614518/100‑1 (MQ=255)
                                                            attTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACttt                                   >  1:4516600/1‑100 (MQ=255)
                                                            attTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACttt                                   >  1:495066/1‑100 (MQ=255)
                                                            attTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACttt                                   >  1:4105959/1‑100 (MQ=255)
                                                             ttttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTg                                  <  2:160128/100‑1 (MQ=255)
                                                             ttttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTg                                  <  1:525570/100‑1 (MQ=255)
                                                              tttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTTTGCCGCCATTGCTGCTGATCCTGCGTCGAACTTTga                                 >  2:5026656/1‑100 (MQ=255)
                                                              tttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTga                                 <  1:1989211/100‑1 (MQ=255)
                                                              tttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTga                                 <  1:1897163/100‑1 (MQ=255)
                                                               ttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgac                                >  1:3727199/1‑100 (MQ=255)
                                                                tttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacg                               <  2:187064/100‑1 (MQ=255)
                                                                tttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacg                               <  1:2248948/100‑1 (MQ=255)
                                                                   cgcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACa                            >  1:1103420/1‑100 (MQ=255)
                                                                   cgcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACa                            <  2:1026204/100‑1 (MQ=255)
                                                                    gcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACAt                           >  2:70042/1‑100 (MQ=255)
                                                                      gggTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGc                         <  1:1372056/100‑1 (MQ=255)
                                                                        gTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCaa                       >  2:33296/1‑100 (MQ=255)
                                                                         tGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAt                      >  2:3140530/1‑100 (MQ=255)
                                                                          gAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAtt                     >  1:3064817/1‑100 (MQ=255)
                                                                           aaaCGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAttt                    >  1:5553322/1‑100 (MQ=255)
                                                                             aCGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTg                  <  2:5353437/100‑1 (MQ=255)
                                                                                aCTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgc               <  1:1217333/100‑1 (MQ=255)
                                                                                 cTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgcg              >  1:2200835/1‑100 (MQ=255)
                                                                                 cTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgcg              <  1:3894878/100‑1 (MQ=255)
                                                                                    cTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTg           >  2:3760200/1‑100 (MQ=255)
                                                                                     tGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGa          <  2:612356/100‑1 (MQ=255)
                                                                                     tGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGa          <  2:3473158/100‑1 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGac         <  2:4039120/100‑2 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGAt         <  2:3832879/100‑1 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGAt         >  2:39365/1‑100 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGAt         <  1:1780618/100‑1 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGAt         <  1:2355555/100‑1 (MQ=255)
                                                                                       gTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATa        <  1:5557969/100‑1 (MQ=255)
                                                                                       gTTCGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATa        >  2:3605697/1‑100 (MQ=255)
                                                                                         ttGTACACAGCCAGCAGTTCGTCCACCTTTTTTTTTTTTTTTCCTTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATAcc      >  2:2457820/1‑100 (MQ=255)
                                                                                            tACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACt   <  2:1114476/100‑1 (MQ=255)
                                                                                            tACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACt   <  1:2215783/100‑1 (MQ=255)
                                                                                            tACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACt   >  1:772913/1‑100 (MQ=255)
                                                                                            tACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACt   <  2:4822812/100‑1 (MQ=255)
                                                                                             acacAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACTc  >  1:4392373/1‑100 (MQ=255)
                                                                                                |                                                                                                
TCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACTC  >  NC_000913/3515019‑3515211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: