Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,568,664 T→G Y222S (TAT→TCT)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,568,6640TG98.3% 590.9 / ‑1.7 174Y222S (TAT→TCT) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (84/86);  total (86/88)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

GCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCG  >  NC_000913/3568568‑3568759
                                                                                                |                                                                                               
gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGt                                                                                              >  2:5418684/1‑100 (MQ=255)
    gACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGc                                                                                          >  2:3794210/1‑100 (MQ=255)
     aCCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCg                                                                                         <  1:2063991/100‑1 (MQ=255)
      ccGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGt                                                                                        >  2:5309609/1‑100 (MQ=255)
      ccGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGt                                                                                        >  2:3368973/1‑100 (MQ=255)
      ccGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGt                                                                                        >  2:1331241/1‑100 (MQ=255)
      ccGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGt                                                                                        >  2:1066442/1‑100 (MQ=255)
       cGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTc                                                                                       <  2:76448/100‑1 (MQ=255)
       cGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTc                                                                                       >  1:116903/1‑100 (MQ=255)
        ggCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCa                                                                                      >  2:1863215/1‑100 (MQ=255)
         gCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCaa                                                                                     >  1:379088/1‑100 (MQ=255)
            tCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCCTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGa                                                                                  <  2:5185514/100‑1 (MQ=255)
             cGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGAc                                                                                 >  2:2470301/1‑100 (MQ=255)
               gTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGt                                                                               >  1:3623862/1‑100 (MQ=255)
               gTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGt                                                                               >  1:1592111/1‑100 (MQ=255)
                tGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTa                                                                              <  2:243854/100‑1 (MQ=255)
                 gATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGTGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAg                                                                             >  1:4686879/1‑100 (MQ=255)
                 gATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAg                                                                             <  2:4476590/100‑1 (MQ=255)
                  aTCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGa                                                                            <  2:3883829/100‑1 (MQ=255)
                  aTCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGa                                                                            <  2:58522/100‑1 (MQ=255)
                   tCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGAt                                                                           >  1:2796257/1‑100 (MQ=255)
                   tCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGAt                                                                           <  2:2322717/100‑1 (MQ=255)
                      tGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCCAAGACGTAGATAcc                                                                        >  2:3812799/1‑100 (MQ=255)
                      tGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATAcc                                                                        <  1:2262213/100‑1 (MQ=255)
                      tGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATAcc                                                                        <  1:1855688/100‑1 (MQ=255)
                      tGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATAcc                                                                        >  1:1527365/1‑100 (MQ=255)
                      tGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCCTCTTCCAGCAGTTCATACAGAGAGTCGGAGTCACAGACGTAGATatc                                                                        >  1:4190783/1‑98 (MQ=255)
                          aaTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATa                                                                    >  1:2642769/1‑100 (MQ=255)
                           aTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATAc                                                                   <  1:4077058/100‑1 (MQ=255)
                           aTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATAc                                                                   >  1:378024/1‑100 (MQ=255)
                            tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACt                                                                  <  1:1705647/100‑1 (MQ=255)
                              aaaTCTGTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACCGAGAGTCGGCGTCAAAGCCGTAGATACCCATACTCg                                                                >  2:304825/1‑100 (MQ=255)
                                aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGcc                                                              <  2:1898436/100‑1 (MQ=255)
                                 tCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCa                                                             <  1:1437760/100‑1 (MQ=255)
                                  cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCag                                                            >  1:1343232/1‑100 (MQ=255)
                                  cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTACAGCAGTTCATACAGAGAGTCGGCGTCAATGACGTAGATACCCATACTCGCCag                                                            <  2:2915478/100‑1 (MQ=255)
                                   tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTGCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCaga                                                           <  1:835217/100‑1 (MQ=255)
                                    ttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCagag                                                          <  2:2575762/100‑1 (MQ=255)
                                     tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAgaga                                                         >  1:2998065/1‑100 (MQ=255)
                                           aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCt                                                   <  2:3543475/100‑1 (MQ=255)
                                           aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCt                                                   <  2:3543411/100‑1 (MQ=255)
                                            gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTc                                                  <  2:2644677/100‑1 (MQ=255)
                                            gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTc                                                  <  2:1766625/100‑1 (MQ=255)
                                             tCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCg                                                 <  2:4631168/100‑1 (MQ=255)
                                             tCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCg                                                 <  2:5347896/100‑1 (MQ=255)
                                              cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCgg                                                <  1:170253/100‑1 (MQ=255)
                                              cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCgg                                                >  1:919691/1‑100 (MQ=255)
                                              cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCgg                                                >  1:1389408/1‑100 (MQ=255)
                                               gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGa                                               >  1:3584625/1‑100 (MQ=255)
                                               gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGa                                               <  2:1148760/100‑1 (MQ=255)
                                               gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCAGACCCGCCAGAGATTTGCTCGGa                                               >  1:5160153/1‑100 (MQ=255)
                                                tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGGGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGAt                                              <  1:3087962/100‑1 (MQ=255)
                                                tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGAt                                              <  1:2617111/100‑1 (MQ=255)
                                                tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGAt                                              <  1:3088574/100‑1 (MQ=255)
                                                 ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAATACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATc                                             >  2:2503763/1‑100 (MQ=255)
                                                 ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATc                                             >  1:1075323/1‑100 (MQ=255)
                                                  gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCg                                            >  2:504484/1‑100 (MQ=255)
                                                      gAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCg                                        <  2:408336/100‑1 (MQ=255)
                                                        gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGc                                      >  1:1222454/1‑100 (MQ=255)
                                                        gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGc                                      >  2:3120650/1‑100 (MQ=255)
                                                        gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGc                                      >  2:4428190/1‑100 (MQ=255)
                                                        gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGc                                      >  2:5138426/1‑100 (MQ=255)
                                                          tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCAt                                    <  2:2014586/100‑1 (MQ=255)
                                                          tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCAt                                    >  2:1387194/1‑100 (MQ=255)
                                                            tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCAttn                                  <  1:4426265/100‑2 (MQ=255)
                                                             cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGa                                 >  1:1917902/1‑100 (MQ=255)
                                                             cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGa                                 >  2:2729565/1‑100 (MQ=255)
                                                              aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAc                                <  2:1623196/100‑1 (MQ=255)
                                                                 gcgATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAcggc                             >  1:1012396/1‑100 (MQ=255)
                                                                  cgATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAcggcg                            >  1:3052401/1‑100 (MQ=255)
                                                                   gATCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCAGACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAcggcgg                           <  2:3260854/100‑1 (MQ=255)
                                                                    aTCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCggg                          <  2:209173/100‑1 (MQ=255)
                                                                    aTCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCggg                          <  1:3514121/100‑1 (MQ=255)
                                                                    aTCGTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCggg                          <  1:5551931/100‑1 (MQ=255)
                                                                    aTCGTCTCCTTCCAGCAGTGCATACGGAGAGTCGGCGGCAAAGAGGTTGGTGGTCATACGGGCCGGGGATTTGCTCGGATCGTTCGGCATTGACGGCggg                          <  2:4321794/100‑1 (MQ=255)
                                                                       gTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTa                       <  2:3011960/100‑1 (MQ=255)
                                                                       gTCTTCTTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTa                       >  1:2373143/1‑100 (MQ=255)
                                                                        tcttctTCCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAg                      >  1:3812038/1‑100 (MQ=255)
                                                                           tcttcCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATTTTCTTTATTTTTTTTTTTTGATCGTTCGGCATTGACGGCGGGTTAGCAg                   >  2:3193594/1‑100 (MQ=255)
                                                                           tcttcCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAg                   >  1:3787656/1‑100 (MQ=255)
                                                                            cttcCAGCAGTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAgg                  >  1:3331542/1‑100 (MQ=255)
                                                                                cagcagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt              >  1:2837373/1‑100 (MQ=255)
                                                                                cagcagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt              <  1:3563750/100‑1 (MQ=255)
                                                                                cagcagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt              <  2:3218790/100‑1 (MQ=255)
                                                                                 agcagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGttttt             <  1:3842241/100‑1 (MQ=255)
                                                                                   cagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCa           <  2:3711118/100‑1 (MQ=255)
                                                                                   cagTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCa           <  1:4896068/100‑1 (MQ=255)
                                                                                    agTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCaa          <  1:1449861/100‑1 (MQ=255)
                                                                                    agTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCaa          <  1:2987996/100‑1 (MQ=255)
                                                                                    agTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCaa          >  2:4008165/1‑100 (MQ=255)
                                                                                     gTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAAc         <  2:43942/100‑1 (MQ=255)
                                                                                     gTTCATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAAc         >  2:4121667/1‑100 (MQ=255)
                                                                                     gTTCATACAGAGAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCGCGGATCGCTCGGCATTGACGGCGGGTTAGCAGGTGTTTCAAc         >  2:3596060/1‑100 (MQ=255)
                                                                                      tccATACAGACAGCCGGCGTCAACGCCGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACg        <  1:253452/98‑1 (MQ=255)
                                                                                        cATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTATCAGGTTTTTCAACGaa      >  2:1373533/1‑100 (MQ=255)
                                                                                        cATACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGaa      <  2:1586599/100‑1 (MQ=255)
                                                                                         aTACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAAt     <  2:4319085/100‑1 (MQ=255)
                                                                                         aTACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAAt     >  1:1259755/1‑100 (MQ=255)
                                                                                          tACAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAAtt    >  1:5462985/1‑100 (MQ=255)
                                                                                            cAGAGAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCAGCATTGACGGCGGGTTAACAGGTTTTTCAACGAATTCg  >  2:2374491/1‑100 (MQ=255)
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GCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCG  >  NC_000913/3568568‑3568759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑