BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,196	T→G	M133L (ATG→CTG)	gntK ←	gluconate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,196	0	T	G	97.4%	660.8 / ‑0.2	190	M133L (ATG→CTG)	gntK	gluconate kinase 2
Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (97/88); total (98/92)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

GCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGATTAC  >  NC_000913/3577099‑3577291

gCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAgtt                                                                                               >  2:774702/1‑100 (MQ=255)

 ccACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTg                                                                                              >  1:5537887/1‑100 (MQ=255)

 ccACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTg                                                                                              <  2:2573295/100‑1 (MQ=255)

 ccACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTg                                                                                              >  2:3350658/1‑100 (MQ=255)

  cacaACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTgg                                                                                             >  2:1824977/1‑100 (MQ=255)

   acaacaCCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTggg                                                                                            <  2:4880644/100‑1 (MQ=255)

    caacaCCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGt                                                                                           >  2:2232245/1‑100 (MQ=255)

    caacaCCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGt                                                                                           >  1:3639991/1‑100 (MQ=255)

      acacCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCGAACTGCGTCACCAACAGTTGGGttt                                                                                         <  1:4978136/100‑1 (MQ=255)

         ccTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTaa                                                                                      >  2:2467445/1‑100 (MQ=255)

          cTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTaaa                                                                                     >  1:4716070/1‑100 (MQ=255)

          cTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTaaa                                                                                     >  2:1545880/1‑100 (MQ=255)

          cTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTaaa                                                                                     <  1:155508/100‑1 (MQ=255)

           ttCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAg                                                                                    >  1:4182581/1‑100 (MQ=255)

           ttCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAg                                                                                    <  2:4338478/100‑1 (MQ=255)

            tCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGa                                                                                   >  2:2676013/1‑100 (MQ=255)

               aGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAAt                                                                                <  1:1532486/100‑1 (MQ=255)

               aGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAAt                                                                                >  1:3100842/1‑100 (MQ=255)

                 cGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATgg                                                                              <  1:3956596/100‑1 (MQ=255)

                  ggTTGATCGATATCCACCACCAGTAC‑TCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGcc                                                                            >  2:1985281/1‑100 (MQ=255)

                    ttGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCt                                                                           <  1:3131846/100‑1 (MQ=255)

                      gATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCACACTGCGTCACCAGCAGTTGGGTGTTAAAGAAATGGCCttg                                                                         >  1:4769084/1‑99 (MQ=255)

                      gATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCttt                                                                         >  1:5055855/1‑100 (MQ=255)

                      gATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCttt                                                                         >  1:1717159/1‑100 (MQ=255)

                       aTCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTg                                                                        >  2:1739712/1‑100 (MQ=255)

                         cGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTgcg                                                                      >  1:3279603/1‑100 (MQ=255)

                         cGATATCCACCACCAGTACATCGGTTTCGGCCGCACCCGGCTCCTGCAGCGTTCCAAACTGCGTCACCAACTGTTGGGTTTTAAACACATGGCCTTTgcg                                                                      <  2:2769625/100‑1 (MQ=255)

                          gATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTgcgc                                                                     <  1:2296625/100‑1 (MQ=255)

                             atCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCt                                                                  <  1:1792156/100‑1 (MQ=255)

                               ncaccaccaGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCttt                                                                >  1:1035935/2‑100 (MQ=255)

                                    accaGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAggc                                                           >  1:617079/1‑100 (MQ=255)

                                    accaGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAggc                                                           >  1:932596/1‑100 (MQ=255)

                                     ccaGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAggcg                                                          >  1:1399838/1‑100 (MQ=255)

                                     ccaGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAggcg                                                          <  2:810589/100‑1 (MQ=255)

                                       agaaCATCGGTAACGTCAACACCCGGAAACTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAggcggc                                                        <  2:1065907/97‑1 (MQ=255)

                                          aCATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCttt                                                     >  2:316663/1‑100 (MQ=255)

                                          aCATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGCTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCttt                                                     >  2:317019/1‑100 (MQ=255)

                                           cATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTc                                                    <  2:1381204/100‑1 (MQ=255)

                                           cATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTc                                                    <  2:43841/100‑1 (MQ=255)

                                            aTCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCa                                                   >  1:1845957/1‑100 (MQ=255)

                                             tCGTTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCaa                                                  <  1:4193100/100‑1 (MQ=255)

                                              cGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGGGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAAt                                                 <  2:5031725/100‑1 (MQ=255)

                                              cGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAAt                                                 <  1:1265761/100‑1 (MQ=255)

                                               ggTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATc                                                >  2:4592687/1‑100 (MQ=255)

                                               ggTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATc                                                <  1:1003902/100‑1 (MQ=255)

                                               ggTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATc                                                <  1:886182/100‑1 (MQ=255)

                                                gTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATca                                               >  2:4857610/1‑100 (MQ=255)

                                                gTTTCGGCCGCCGCCGGCTCCGGCAGGGTTTCAAGCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCCGGCGGCTTTCAATca                                               <  2:4180890/100‑1 (MQ=255)

                                                 tttCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATcac                                              <  1:862484/100‑1 (MQ=255)

                                                    cGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATc                                           >  2:3625822/1‑100 (MQ=255)

                                                    cGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATc                                           >  2:3103547/1‑100 (MQ=255)

                                                     gTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCa                                          <  1:4776885/100‑1 (MQ=255)

                                                      tCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCaa                                         >  1:972286/1‑100 (MQ=255)

                                                           aCCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACCGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCg                                    <  1:1357325/100‑1 (MQ=255)

                                                           aCCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCg                                    <  1:3545290/100‑1 (MQ=255)

                                                            cccGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGCTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATTAAAATCGc                                   >  2:2168203/1‑100 (MQ=255)

                                                              cGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCt                                 <  1:3734476/100‑1 (MQ=255)

                                                              cGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCt                                 <  2:3431750/100‑1 (MQ=255)

                                                              cGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCt                                 <  1:2567971/100‑1 (MQ=255)

                                                              cGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCt                                 >  1:2650678/1‑100 (MQ=255)

                                                                gCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCttt                               <  2:1580968/100‑1 (MQ=255)

                                                                 cTCCTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTc                              <  1:1845679/100‑1 (MQ=255)

                                                                   ccTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCaa                            <  2:2873098/100‑1 (MQ=255)

                                                                    cTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCATAATCGCCTTTCaaa                           >  2:3313883/1‑100 (MQ=255)

                                                                    cTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCaaa                           >  2:1946887/1‑100 (MQ=255)

                                                                    cTGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCaaa                           >  2:2360029/1‑100 (MQ=255)

                                                                     tGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAAt                          >  1:2055179/1‑100 (MQ=255)

                                                                     tGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAAt                          <  2:1266141/100‑1 (MQ=255)

                                                                     tGCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAAt                          <  2:2710252/100‑1 (MQ=255)

                                                                      gCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATa                         >  1:1044845/1‑100 (MQ=255)

                                                                      gCAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATa                         <  1:2491034/100‑1 (MQ=255)

                                                                       cAGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAg                        <  1:4939542/100‑1 (MQ=255)

                                                                        aGCGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCGTTCAAATAGa                       <  1:4868904/100‑1 (MQ=255)

                                                                          cGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATg                     <  2:5027486/100‑1 (MQ=255)

                                                                          cGTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATg                     <  1:1956600/100‑1 (MQ=255)

                                                                           gTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGa                    >  1:1884687/1‑100 (MQ=255)

                                                                           gTTTCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGa                    >  2:1714110/1‑100 (MQ=255)

                                                                             ttCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGaaa                  <  2:14546/100‑1 (MQ=255)

                                                                             ttCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGaaa                  >  2:5339970/1‑100 (MQ=255)

                                                                              tCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAag                 >  2:3746235/1‑100 (MQ=255)

                                                                              tCAAACTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAag                 <  2:920330/100‑1 (MQ=255)

                                                                                aaaCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATAACATCAAAATCGCCTTTCAAATAGATGAAagag               >  1:3660623/1‑100 (MQ=255)

                                                                                 aaCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGTCGGCTTTCAATCACATCAAAATCGCCTTTCAAATGGATGAAagata              <  2:5102417/100‑3 (MQ=255)

                                                                                 aaCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAagaga              >  1:3781860/1‑100 (MQ=255)

                                                                                 aaCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAagaga              >  1:4043600/1‑100 (MQ=255)

                                                                                 aaCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAagaga              <  1:1473854/100‑1 (MQ=255)

                                                                                  aCTGCGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAagagag             >  1:3603009/1‑100 (MQ=255)

                                                                                      cGTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTc         >  1:4823175/1‑100 (MQ=255)

                                                                                       gTCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCg        >  1:2821382/1‑100 (MQ=255)

                                                                                        tCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAGAGAGAGATTCgg       <  2:748954/100‑1 (MQ=255)

                                                                                        tCACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCgg       >  2:3915930/1‑100 (MQ=255)

                                                                                         cACCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGa      >  2:3761331/1‑100 (MQ=255)

                                                                                          aCCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAt     >  1:788174/1‑100 (MQ=255)

                                                                                          aCCAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAt     >  1:1114770/1‑100 (MQ=255)

                                                                                           ccAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAtt    >  2:4726033/1‑100 (MQ=255)

                                                                                           ccAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAtt    <  2:5271356/100‑1 (MQ=255)

                                                                                           ccAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAtt    >  2:2043828/1‑100 (MQ=255)

                                                                                           ccAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAtt    >  1:5343628/1‑100 (MQ=255)

                                                                                           ccAACAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGAtn    <  1:4450194/100‑2 (MQ=255)

                                                                                             aaCAGTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGATTAc  <  1:662848/100‑1 (MQ=255)

GCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGGCGGCTTTCAATCACATCAAAATCGCCTTTCAAATAGATGAAAGAGAGATTCGGATTAC  >  NC_000913/3577099‑3577291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`