Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,602,989 T→G I419L (ATT→CTT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,602,9890TG98.3% 634.4 / ‑3.1 178I419L (ATT→CTT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (91/83);  total (93/85)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

GTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCAG  >  NC_000913/3602892‑3603085
                                                                                                 |                                                                                                
gtgccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAagag                                                                                                <  1:2240846/100‑1 (MQ=255)
  gccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTc                                                                                              >  2:4760674/1‑100 (MQ=255)
  gccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTc                                                                                              >  1:151092/1‑100 (MQ=255)
  gccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTc                                                                                              <  2:104139/100‑1 (MQ=255)
    cgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAg                                                                                            <  2:2662979/100‑1 (MQ=255)
     gtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGc                                                                                           <  2:149616/100‑1 (MQ=255)
      tCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCa                                                                                          <  1:2138958/100‑1 (MQ=255)
         aGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATaa                                                                                       >  1:773750/1‑100 (MQ=255)
         aGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATaa                                                                                       >  2:4032092/1‑100 (MQ=255)
          gTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAAc                                                                                      >  2:47826/1‑100 (MQ=255)
           tttCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACt                                                                                     <  2:3844844/100‑1 (MQ=255)
              cGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCa                                                                                  <  2:3740669/100‑1 (MQ=255)
              cGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCa                                                                                  >  1:75000/1‑100 (MQ=255)
              cGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCa                                                                                  <  1:2345216/100‑1 (MQ=255)
              cGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCa                                                                                  >  1:2404194/1‑100 (MQ=255)
               gTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCAt                                                                                 >  1:607422/1‑100 (MQ=255)
               gTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCAt                                                                                 >  2:4418581/1‑100 (MQ=255)
                 tAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATgc                                                                               <  1:3189778/100‑1 (MQ=255)
                 tAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTAACCGCGTTCTGCCCGGTGCTGGCCTCAAGAGTCAGCATAACTTAATga                                                                               >  2:1355706/1‑99 (MQ=255)
                  aGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCGCGGTGCTGGCATCAAGCGTCAGCATAACTTCATgcg                                                                              <  2:3535100/100‑1 (MQ=255)
                    cGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATgcggc                                                                            <  2:3365924/100‑1 (MQ=255)
                    cGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATgcggc                                                                            <  2:2563273/100‑1 (MQ=255)
                      tGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGgcgc                                                                          >  1:2380644/1‑100 (MQ=255)
                       gATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCt                                                                         <  1:2507271/100‑1 (MQ=255)
                        aTGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCtt                                                                        <  2:3132330/100‑1 (MQ=255)
                            cGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAAc                                                                    >  1:4066158/1‑100 (MQ=255)
                            cGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAAc                                                                    <  1:3501842/100‑1 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACg                                                                   >  2:3993856/1‑100 (MQ=255)
                                tAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCg                                                                <  1:3273041/100‑1 (MQ=255)
                                tAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCg                                                                <  2:3766918/100‑1 (MQ=255)
                                   gCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGt                                                             >  2:1240519/1‑100 (MQ=255)
                                    ccAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGACGCTTCAACGTCGAGtt                                                            >  1:5128235/1‑100 (MQ=255)
                                      aaCGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                                                          >  1:3311410/1‑100 (MQ=255)
                                      aaCGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                                                          >  2:5201827/1‑100 (MQ=255)
                                       aCGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                                                         >  2:2056931/1‑100 (MQ=255)
                                       aCGGCTTCATGGAACAGTGTGGCCTGGCTTACCGCGTTCTGCCCGGTTCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                                                         <  2:1904831/100‑1 (MQ=255)
                                       aCGGCTTCACGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                                                         <  1:1741576/100‑1 (MQ=255)
                                        cGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                                                        <  1:1192605/100‑1 (MQ=255)
                                        cGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                                                        <  1:10437/100‑1 (MQ=255)
                                         ggCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                                                       <  1:5467424/100‑1 (MQ=255)
                                         ggCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                                                       <  2:1111276/100‑1 (MQ=255)
                                          gCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttag                                                      >  1:3830397/1‑98 (MQ=255)
                                           cTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcat                                                     >  2:343351/1‑100 (MQ=255)
                                           cTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcat                                                     >  1:3900031/1‑100 (MQ=255)
                                           cTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcat                                                     >  1:2806636/1‑100 (MQ=255)
                                            ttCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatc                                                    >  1:4014486/1‑100 (MQ=255)
                                             tCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatca                                                   >  2:41933/1‑100 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                >  2:270149/1‑100 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                <  2:1726798/100‑1 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                >  1:2744954/1‑100 (MQ=255)
                                                    aCAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGAc                                            <  1:1287210/100‑1 (MQ=255)
                                                    aCAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGAc                                            <  2:2011528/100‑1 (MQ=255)
                                                     cAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACg                                           >  1:1361323/1‑100 (MQ=255)
                                                      aGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATACCTTCATGCGGCGCTTCAACGTCGAGTCTCTTCATCACGCGGACGa                                          >  1:5214196/1‑100 (MQ=255)
                                                      aGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGa                                          <  2:5459433/100‑1 (MQ=255)
                                                       gTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt                                         >  1:845180/1‑100 (MQ=255)
                                                       gTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt                                         >  2:5378060/1‑100 (MQ=255)
                                                       gTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt                                         >  2:4243291/1‑100 (MQ=255)
                                                       gTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt                                         <  2:4266946/100‑1 (MQ=255)
                                                         ttGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAttt                                       <  2:4082766/100‑1 (MQ=255)
                                                          tGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtttt                                      >  1:4033004/1‑100 (MQ=255)
                                                            gCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct                                    >  2:5116946/1‑100 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  2:816330/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  >  2:222669/1‑100 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  2:632918/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  1:5533899/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCGGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  1:3577488/100‑1 (MQ=255)
                                                               tGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCa                                 <  1:392541/100‑1 (MQ=255)
                                                                 gCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAc                               <  1:3390374/100‑1 (MQ=255)
                                                                  cTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAct                              >  1:3209492/1‑100 (MQ=255)
                                                                  cTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAct                              >  1:2502386/1‑100 (MQ=255)
                                                                  cTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAct                              >  1:1423921/1‑100 (MQ=255)
                                                                    tACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAActct                            <  2:2154508/100‑1 (MQ=255)
                                                                     aCCGAGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCtt                           >  2:4642154/1‑100 (MQ=255)
                                                                      ccGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTc                          >  2:298577/1‑100 (MQ=255)
                                                                      ccGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTc                          >  2:358270/1‑100 (MQ=255)
                                                                       cgcgTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTcc                         >  1:4595533/1‑100 (MQ=255)
                                                                          gTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATc                      >  2:3778246/1‑100 (MQ=255)
                                                                            tCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAg                    <  1:3679687/100‑1 (MQ=255)
                                                                             cTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGCTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCggg                   >  1:2270425/1‑100 (MQ=255)
                                                                               gCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGGGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTg                 >  2:5075413/1‑100 (MQ=255)
                                                                                    gTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGAtt            <  1:5104484/100‑1 (MQ=255)
                                                                                    gTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGAtt            <  1:1688676/100‑1 (MQ=255)
                                                                                    gTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGAtt            <  2:2779775/100‑1 (MQ=255)
                                                                                       cTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGt         >  1:1541444/1‑100 (MQ=255)
                                                                                       cTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGt         >  1:5004605/1‑100 (MQ=255)
                                                                                       cTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGt         <  2:402284/100‑1 (MQ=255)
                                                                                         ggCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTc       <  1:4282520/100‑1 (MQ=255)
                                                                                         ggCATCAAGAGTCAGCATAACTTCATGAGGCGCTTCCACGTCGAGTTTCTTCATCACGCGTACGATTTTCTTCATATCTTCAATCAGGTAAGATATGTTc       >  2:2476278/1‑100 (MQ=255)
                                                                                          gCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCt      <  2:270778/100‑1 (MQ=255)
                                                                                           cATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTg     >  2:1651203/1‑100 (MQ=255)
                                                                                           cATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTg     <  1:5406268/100‑1 (MQ=255)
                                                                                            aTCAAGAGTCAGCATAACTTCATGCTGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGc    <  2:2316723/100‑1 (MQ=255)
                                                                                            aTCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTgn    <  1:2638060/100‑2 (MQ=255)
                                                                                            aTCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGc    >  2:496853/1‑100 (MQ=255)
                                                                                             tCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAATTCTTCCATCAGGTGCGATTTGTTCTGCa   >  2:2460236/1‑100 (MQ=255)
                                                                                             tCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTGTTCCATCAGGTGCGTTGGGTTCTGCa   >  1:5575815/1‑100 (MQ=255)
                                                                                             tCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCa   >  2:2444736/1‑100 (MQ=255)
                                                                                             tCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCa   <  1:4625337/100‑1 (MQ=255)
                                                                                              cAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCAg  >  1:3264027/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCAG  >  NC_000913/3602892‑3603085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑