BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,996	A→C	L89* (TTA→TGA)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,996	0	A	C	99.1%	826.0 / ‑4.6	233	L89* (TTA→TGA)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (122/109); total (123/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTAAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTT  >  NC_000913/3689899‑3690092

cATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTcaa                                                                                                <  1:1020816/100‑1 (MQ=255)

 aTCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAg                                                                                               <  2:4082934/100‑1 (MQ=255)

 aTCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAg                                                                                               <  2:4082401/100‑1 (MQ=255)

 aTCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAg                                                                                               >  2:2122673/1‑100 (MQ=255)

 aTCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAg                                                                                               >  1:4813842/1‑100 (MQ=255)

   ccAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGaa                                                                                             <  2:5346388/100‑1 (MQ=255)

   ccAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGaa                                                                                             >  2:1457219/1‑100 (MQ=255)

     aGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGCAcc                                                                                           >  1:4398629/1‑100 (MQ=255)

     aGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAAcc                                                                                           <  1:3378633/100‑1 (MQ=255)

      gACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAAccc                                                                                          <  2:1230748/100‑1 (MQ=255)

      gACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAAccc                                                                                          >  2:851997/1‑100 (MQ=255)

        cATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTg                                                                                        >  2:4446882/1‑100 (MQ=255)

        cATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTg                                                                                        >  1:3047041/1‑100 (MQ=255)

         aTCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGa                                                                                       >  1:3106627/1‑100 (MQ=255)

          tCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACGTACTGTTCTCTTTCTGGCCCCACAGCCAGGCGGGCAAACGTTCTTTGCAAGAACCCTGAg                                                                                      >  2:1625560/1‑100 (MQ=255)

           cACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGc                                                                                     >  2:3393190/1‑100 (MQ=255)

           cACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGc                                                                                     >  2:2228880/1‑100 (MQ=255)

           cACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGc                                                                                     <  2:5248103/100‑1 (MQ=255)

           cACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGc                                                                                     >  1:3983246/1‑100 (MQ=255)

            aCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCg                                                                                    >  1:196543/1‑100 (MQ=255)

             ccATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCga                                                                                   <  1:3690806/100‑1 (MQ=255)

              cATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGTGCAAACGTTCTTTTCAAGAACCCTGAGCgag                                                                                  <  1:4464757/100‑1 (MQ=255)

              cATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCgag                                                                                  >  2:3093206/1‑100 (MQ=255)

                tCGGATGCCGAATTGCTGTCCAGGACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGAt                                                                                <  2:1891297/100‑1 (MQ=255)

                  ggAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTg                                                                              <  1:1819885/100‑1 (MQ=255)

                   gAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGt                                                                             <  2:3957066/100‑1 (MQ=255)

                      gCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCt                                                                          >  1:651972/1‑100 (MQ=255)

                       ccGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTg                                                                         >  2:2545886/1‑100 (MQ=255)

                       ccGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTg                                                                         <  1:2571149/100‑1 (MQ=255)

                       ccGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTg                                                                         >  1:5120934/1‑100 (MQ=255)

                        cGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGc                                                                        >  1:2202077/1‑100 (MQ=255)

                        cGAATTGCTGTCCAGCACTTCCAACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGc                                                                        >  1:4657780/1‑100 (MQ=255)

                         gAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCg                                                                       <  2:3853831/100‑1 (MQ=255)

                           aTTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTc                                                                     <  1:2165086/100‑1 (MQ=255)

                            ttGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTcc                                                                    <  1:1388407/100‑1 (MQ=255)

                              gCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAg                                                                  >  1:4729610/1‑100 (MQ=255)

                              gCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAg                                                                  >  1:5066160/1‑100 (MQ=255)

                               cTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGt                                                                 >  1:2952028/1‑100 (MQ=255)

                               cTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGt                                                                 <  2:2926629/100‑1 (MQ=255)

                                tGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTc                                                                <  2:1515666/100‑1 (MQ=255)

                                tGTCCAGCACTTCCCACTTACTGCTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTTAGCGAGATTGTTCTGCGTCCAGTc                                                                <  2:2044573/100‑1 (MQ=255)

                                 gTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGCGATTGTTCTGCGTCCAGTCg                                                               >  2:2100565/1‑100 (MQ=255)

                                 gTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCg                                                               <  2:4202637/100‑1 (MQ=255)

                                 gTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCg                                                               >  1:5530242/1‑100 (MQ=255)

                                    cAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCgaga                                                            >  1:2890956/1‑100 (MQ=255)

                                     aGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGaa                                                           <  2:4961158/100‑1 (MQ=255)

                                     aGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGaa                                                           <  2:1395505/100‑1 (MQ=255)

                                      gCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAat                                                          >  2:4865401/1‑100 (MQ=255)

                                      gCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAat                                                          <  2:4046224/100‑1 (MQ=255)

                                       cACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAata                                                         <  2:3450929/100‑1 (MQ=255)

                                        aCTTCCCACTTACTGTTCTCTTTCTGGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAatat                                                        <  2:2608681/100‑1 (MQ=255)

                                         cTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAAtatt                                                       >  1:1539959/1‑100 (MQ=255)

                                         cTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAAtatt                                                       <  2:77732/100‑1 (MQ=255)

                                          ttCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAAtatta                                                      <  1:5332631/100‑1 (MQ=255)

                                            cccACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATc                                                    >  1:695911/1‑100 (MQ=255)

                                            cccACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATc                                                    >  2:3697498/1‑100 (MQ=255)

                                             ccACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCg                                                   >  2:2473370/1‑100 (MQ=255)

                                                cTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGaaa                                                <  2:2281239/100‑1 (MQ=255)

                                                    cTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTg                                            <  2:4501867/100‑1 (MQ=255)

                                                    cTGTTCTCTTTCTTACCCCACCGCCAGGCGGGCAAACGTTCTTTACAAGACCCTTAAGCGAGATTGTTCTGCGTCCAGTCGTGAATATTATCGAAAGCTg                                            <  2:545500/100‑1 (MQ=255)

                                                      gTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCa                                          <  1:4314017/100‑1 (MQ=255)

                                                       ttCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCAc                                         <  1:4266948/100‑1 (MQ=255)

                                                         ctctTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACgg                                       <  1:4883521/100‑1 (MQ=255)

                                                           ctttcttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTc                                     >  2:2963658/1‑100 (MQ=255)

                                                           ctttcttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTc                                     >  2:3548986/1‑100 (MQ=255)

                                                            tttcttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCg                                    >  1:3747483/1‑100 (MQ=255)

                                                             ttcttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGt                                   <  2:402639/100‑1 (MQ=255)

                                                               cttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                 >  1:1150402/1‑100 (MQ=255)

                                                               cttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                 >  1:2344624/1‑100 (MQ=255)

                                                                ttGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAg                                <  1:4862411/100‑1 (MQ=255)

                                                                 tGCCCCTCAGCCAGGTGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgc                               <  2:1113872/100‑1 (MQ=255)

                                                                 tGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgc                               <  2:4357516/100‑1 (MQ=255)

                                                                  gCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgcc                              >  2:2837490/1‑100 (MQ=255)

                                                                  gCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgcc                              >  1:3884247/1‑100 (MQ=255)

                                                                  gCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgcc                              >  2:1871840/1‑100 (MQ=255)

                                                                   ccccACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgccg                             <  1:1066826/100‑1 (MQ=255)

                                                                    cccACAGCCAGGCGGGCAAACGTTCTTTTCAAGCACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgccgc                            <  1:1860458/100‑1 (MQ=255)

                                                                    cccACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATCTTATCCAAAGCTGCACGGTCGTTAgccgc                            >  1:1597106/1‑100 (MQ=255)

                                                                    cccACAGCCAGGCGGGCAAACGTTCTTTTAAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAgccgc                            >  2:3759640/1‑100 (MQ=255)

                                                                      cacaGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCa                          >  1:5557973/1‑100 (MQ=255)

                                                                       acaGCCAGGCGGGCAAACGTTCTTTTCAAGCACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAg                         <  2:3143500/100‑1 (MQ=255)

                                                                       acaGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAg                         <  2:2660441/100‑1 (MQ=255)

                                                                       acaGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAg                         >  1:3327270/1‑100 (MQ=255)

                                                                       acaGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAg                         <  2:1343559/100‑1 (MQ=255)

                                                                        caGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAgg                        <  2:4699287/100‑1 (MQ=255)

                                                                        caGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAgg                        >  1:5391026/1‑100 (MQ=255)

                                                                          gCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGc                      >  1:4757590/1‑100 (MQ=255)

                                                                          gCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGc                      <  1:2274715/100‑1 (MQ=255)

                                                                             aGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCaga                   <  1:4092821/100‑1 (MQ=255)

                                                                              ngCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCagag                  >  1:2299476/2‑100 (MQ=255)

                                                                              ggCGTGCAAACGTTCTTTTCAAGAACGCTGAGCGAGATTGTTCTGCGTCCAGTCAAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCagag                  <  2:2179274/100‑1 (MQ=255)

                                                                              ggCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCagag                  <  1:948285/100‑1 (MQ=255)

                                                                                  ggCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTCCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACa              <  2:4996030/100‑1 (MQ=255)

                                                                                   tcAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACAt             <  2:1766361/99‑1 (MQ=255)

                                                                                   gCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACAt             <  2:5398265/100‑1 (MQ=255)

                                                                                   gCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACAt             <  1:2612638/100‑1 (MQ=255)

                                                                                    cAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACAta            >  1:1786726/1‑99 (MQ=255)

                                                                                     aaaCGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGc           <  2:4746061/100‑1 (MQ=255)

                                                                                       aCGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCg         <  2:1028474/100‑1 (MQ=255)

                                                                                         gTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTa       >  1:2668859/1‑100 (MQ=255)

CATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTAAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTT  >  NC_000913/3689899‑3690092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`